Barley chymotrypsin inhibitor-2 （CI-2） was considered to be a promising candidate for enhancing the nutritional value of other cereals by increasing its concentration as it is rich in lysine than any other storage p...Barley chymotrypsin inhibitor-2 （CI-2） was considered to be a promising candidate for enhancing the nutritional value of other cereals by increasing its concentration as it is rich in lysine than any other storage protein. Also, it was proposed that CI-2 might play an important role in the inhibition of proteolytic enzymes from pests or pathogens as CI-2 can strongly inhibit chymotrypsin and subtilisin. In this study, a total of 93 CI-2 gene sequences were isolated from wild and cultivated barley. 48 SNPs and 4 indels were detected across the entire sequences. The frequency of SNPs in the noncoding region （1 out of 9 bases） was slightly higher than that in the coding region （1 out of 10.7 bases）. In all, 33.3% of the candidate cSNPs resulted in amino acid changes. As a total, the 24 cSNPs resulted in 15 amino acid changes. Ten distinguishable haplotypes were detected, among which 3 haplotypes were shared in the most barley accessions, whereas the rest of the haplotypes appeared at a lower frequency. In addition, three haplotypes （haplotype 4, 8, and 9） were unique for single accessions. These results suggested that low diversity at the CI-2 locus was detected among the cultivated and wild barley.展开更多
The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed t...The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.展开更多
蛋白酶抑制剂在鳞翅目昆虫的生长、发育以及免疫过程中发挥了重要作用.该文采用非变性聚丙烯酰胺凝胶电泳（Native-polyacrylamide gel electrophoresis Native-PAGE）结合胰凝乳蛋白酶抑制剂（Chymotrypsin inhibitor,CI）和胰蛋白酶...蛋白酶抑制剂在鳞翅目昆虫的生长、发育以及免疫过程中发挥了重要作用.该文采用非变性聚丙烯酰胺凝胶电泳（Native-polyacrylamide gel electrophoresis Native-PAGE）结合胰凝乳蛋白酶抑制剂（Chymotrypsin inhibitor,CI）和胰蛋白酶抑制剂（Trypsin inhibitor,TI）活性染色的方法,调查了棉铃虫部分组织中胰凝乳蛋白酶抑制剂和胰蛋白酶抑制剂的分布情况.结果表明：在碱性非变性聚丙烯酰胺凝胶电泳下,血液中可检测到5条CI活性带,分别命名为H-CI1~H-CI5.同样能检测到几条迁移率很近且难以分开的TI活性带,而在头、丝腺、脂肪体、体壁、中肠和生殖腺等组织器官中没有检测到CI和TI活性带.在酸性非变性聚丙烯酰胺凝胶电泳下,血液中检测到一条CI活性带,命名为H-CIb1;两条TI活性带,命名为H-TI1和H-TI2.其他组织与碱性电泳的结果一致,未检测到CI和TI的存在.展开更多
基金supported by the National Key Technology R&D Program of China (2006BAD01A02-23,2006 BAD13B02-06)the National High Technology Research and Development Program of China (863 Program 2006AA10Z179,2006AA10Z1F8)the Foundation for the Author of National Excellent Doctoral Dissertation of China (200357)
文摘Barley chymotrypsin inhibitor-2 （CI-2） was considered to be a promising candidate for enhancing the nutritional value of other cereals by increasing its concentration as it is rich in lysine than any other storage protein. Also, it was proposed that CI-2 might play an important role in the inhibition of proteolytic enzymes from pests or pathogens as CI-2 can strongly inhibit chymotrypsin and subtilisin. In this study, a total of 93 CI-2 gene sequences were isolated from wild and cultivated barley. 48 SNPs and 4 indels were detected across the entire sequences. The frequency of SNPs in the noncoding region （1 out of 9 bases） was slightly higher than that in the coding region （1 out of 10.7 bases）. In all, 33.3% of the candidate cSNPs resulted in amino acid changes. As a total, the 24 cSNPs resulted in 15 amino acid changes. Ten distinguishable haplotypes were detected, among which 3 haplotypes were shared in the most barley accessions, whereas the rest of the haplotypes appeared at a lower frequency. In addition, three haplotypes （haplotype 4, 8, and 9） were unique for single accessions. These results suggested that low diversity at the CI-2 locus was detected among the cultivated and wild barley.
基金the National Natural Science Foundation of China (Grant Nos. 39730730 and 39770574)Foundation for University Leading Teacher by the Ministry of Education of China (Grant No.GG-230-10625-1065)+1 种基金the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,The Ministry of Education of Chinathe Science Research Foundation of Ministry of Education of Japan (Grant No. 09045069)
文摘The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.
