Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packag...Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packaging of circLIFR expression plasmids to construct stable circLIFR high expression HCC cells.The lentivirus infected with circLIFR high expression sequence was used as the circLIFR high expression group,the lentivirus infected with circLIFR high expression empty vector sequence was used as the negative control group,and the uninfected group was used as the blank control group.After that,circLIFR expression levels were detected by qPCR,and the back splice sites were identified by Sanger sequencing.The cell viability was examined by cell proliferation kit and invasive ability was determined by Transwell assay.Results:The qPCR and Sanger sequencing showed that the stable circLIFR expression of Hep3B cells was successfully established.The circLIFR high expression group had better cell proliferation viability than the negative control and blank control groups,and the differences were statistically significant(P<0.05).The number of cells crossing Matrigel gel in the negative control and blank control groups was(270.8±18.9)and(266.2±17.6),respectively,while the number of cells crossing Matrigel gel in the circLIFR high expression group was(396.6±32.9),and the differences were statistically significant(P<0.05).Conclusion:High circLIFR expression considerably promotes the proliferation and invasive ability of Hep3B cells.展开更多
基金Research and Development Key Project of Hainan Province(No.ZDYF2020134,ZDYF2022SHFZ283)the National Natural Science Foundation of China(No.81660489)+1 种基金the Special Research Fund of The Innovation Platform for Academicians of Hannan Province(No.YSPTZX202005)Innovative Research Project for Postgraduates of Hainan Province(No.Hys2020-355)。
文摘Objective:To investigate the effect of lentiviral stable high expression of circLIFR on the biological behavior of Hep3B hepatocellular carcinoma(HCC)cells.Methods:Hep3B cell lines were infected with lentiviral packaging of circLIFR expression plasmids to construct stable circLIFR high expression HCC cells.The lentivirus infected with circLIFR high expression sequence was used as the circLIFR high expression group,the lentivirus infected with circLIFR high expression empty vector sequence was used as the negative control group,and the uninfected group was used as the blank control group.After that,circLIFR expression levels were detected by qPCR,and the back splice sites were identified by Sanger sequencing.The cell viability was examined by cell proliferation kit and invasive ability was determined by Transwell assay.Results:The qPCR and Sanger sequencing showed that the stable circLIFR expression of Hep3B cells was successfully established.The circLIFR high expression group had better cell proliferation viability than the negative control and blank control groups,and the differences were statistically significant(P<0.05).The number of cells crossing Matrigel gel in the negative control and blank control groups was(270.8±18.9)and(266.2±17.6),respectively,while the number of cells crossing Matrigel gel in the circLIFR high expression group was(396.6±32.9),and the differences were statistically significant(P<0.05).Conclusion:High circLIFR expression considerably promotes the proliferation and invasive ability of Hep3B cells.
