Circular RNA_0015278与甲状腺乳头状癌及临床特征的相关性

目的探讨Circular RNA_0015278(circ_0015278)与甲状腺乳头状癌(PTC)及其临床病理特征的相关性。方法回顾分析手术切除的甲状腺乳头状癌患者200例,采用逆转录定量聚合酶链反应(RT-qPCR)检测Circular RNA_0015278在甲状腺乳头状癌(PTC)及癌旁组织的表达。结果与癌旁组织比较,甲状腺乳头状癌(PTC)中Circular RNA_0015278表达显著降低(Z=-12.122,P<0.001),ROC分析显示,以相对表达量0.743为截断值,可较好的鉴别PTC肿瘤及癌旁组织(AUC:0.903,95%CI:0.873~0.933)。而PTC肿瘤中Circular RNA_0015278的高表达与患者年龄<45岁(Z=-2.319,P=0.020)、无甲状腺外侵犯(Z=-2.042,P=0.041)、无淋巴结转移(Z=-2.494,P=0.013)及低pTNM分期(Z=-3.719,P=0.001)相关(P<0.05)。而未发现与性别(Z=-1.323,P=0.186)、肿瘤大小(Z=-1.273,P=0.203)具有相关性(P>0.05)。结论PTC肿瘤中Circular RNA_0015278的表达显著降低,而其在PTC肿瘤中高表达与患者年龄<45岁、无甲状腺外侵犯、无淋巴结转移及低pTNM分期具有相关性。

Increased circulating circular RNA103516 is a novel biomarker for inflammatory bowel disease in adult patients

BACKGROUND Increasing evidence demonstrates that by acting as microRNA sponges modulating gene expression at the transcriptional or post-transcriptional level,circular RNAs(circRNAs)participate in the pathogenesis of a variety of diseases and are considered ideal biomarkers of human disease.AIM To examine the expression of circRNA_103516 in inflammatory bowel disease(IBD)and its associations with clinical phenotypes and inflammatory cytokines.METHODS Peripheral blood mononuclear cells(PBMCs)were obtained from patients with IBD,healthy controls(HCs),and patient controls(PCs).Expression of circRNA_103516 and hsa-miR-19b-1-5p was assessed by quantitative reverse transcription-polymerase chain reaction.Crohn's disease activity index(CDAI),Mayo score,C-reactive protein(CRP)level,and erythrocyte sedimentation rate(ESR)were measured.To assess the inflammatory cytokines tumour necrosis factorα(TNF-α),interferon-γ(IFN-γ),and interleukin-10(IL-10),blood samples were analysed by flow cytometry.RESULTS Ninety Crohn’s disease(CD)and 90 ulcerative colitis(UC)patients,80 HCs,and 35 PCs were included in the study.CircRNA_103516 was upregulated in CD and UC patients compared with HCs and PCs(P<0.05).The area under the curve of circRNA_103516 for diagnosing CD and UC was 0.790 and 0.687,respectively.In addition,circRNA_103516 levels were increased in active CD and UC compared with remittent groups(P=0.027,P=0.045).Furthermore,in CD,circRNA_103516 correlated positively with CDAI(P<0.001),CRP(P<0.001),ESR(P<0.001),TNFα(P<0.001),and IFN-γ(P<0.001)and negatively correlated with IL-10(P=0.006).In UC patients,circRNA_103516 correlated with Mayo score(P<0.001),CRP(P<0.001),ESR(P<0.001),TNFα(P<0.001),IFN-γ(P=0.011),and IL-10(P=0.002).Additionally,circRNA_103516 correlated positively with stricturing(P=0.018)and penetrating(P=0.031)behaviour.Moreover,hsamiR-19b-1-5p correlated negatively with circRNA_103516 in CD.CONCLUSION CircRNA_103516 levels in PBMCs can be considered an ideal candidate biomarker for diagnosing IBD.Dysregulation of circRNA_103516 may participate in the molecular mechanism of IBD through hsa-miR-19b-1-5p sponging.

circ_0009910对结直肠癌细胞增殖、凋亡的影响及机制

目的探讨环状RNA_0009910(circ_0009910)对结直肠癌细胞(SW620)增殖、凋亡的影响及机制。方法常规培养SW620细胞,将细胞随机分组并进行转染,si-NC组转染si-NC、si-circ_0009910组转染si-circ_0009910、pcDNA组转染pcDNA、pcDNA-circ_0009910组转染pcDNA-circ_0009910、miR-NC组转染miR-NC、miR-198组转染miR-198 mimic、si-circ_0009910+anti-miR-NC组共转染si-circ_0009910和anti-miR-NC、si-circ_0009910+anti-miR-198组共转染si-circ_0009910和miR-198 inhibitor。转染48 h,用qRT-PCR检测细胞中circ_0009910、miR-198表达,用MTT法检测细胞增殖能力,用Annexin V-FITC/PI双染法检测细胞凋亡能力,用Western blotting法检测细胞核相关抗原Ki-67(Ki-67)、B细胞淋巴瘤/白血病-2(Bcl-2)蛋白、Bcl-2相关X蛋白(Bax)表达,采用双荧光素酶报告实验验证circ_0009910的靶基因。结果与si-NC组比较,si-circ_0009910组细胞增殖能力低,细胞凋亡率高,Ki-67、Bcl-2蛋白相对表达量低,Bax蛋白相对表达量高(P均<0.05)。Circular RNA Interactome软件预测出circ_0009910的部分碱基序列可与miR-198形成互补配对。与miR-NC组比较,miR-198组转染WT-circ_0009910后荧光素酶活性低(P<0.05);而两组转染MUT-circ_0009910后荧光素酶活性比较差异无统计学意义(P>0.05)。与pcDNA组比较,pcDNA-circ_0009910组miR-198相对表达量低(P<0.05);与si-NC组比较,si-circ_0009910组miR-198相对表达量高(P<0.05);与miR-NC组比较,miR-198组SW620细胞增殖能力低,细胞凋亡率高,Ki-67、Bcl-2蛋白相对表达量低,Bax蛋白相对表达量高(P均<0.05);与si-circ_0009910+anti-miR-NC组比较,si-circ_0009910+anti-miR-198组细胞增殖能力高,细胞凋亡率低,Ki-67、Bcl-2蛋白相对表达量高,Bax蛋白相对表达量低(P均<0.05)。结论circ_0009910低表达通过靶向调控miR-198的表达抑制SW620细胞增殖,促进其促凋亡。