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Circulating tumor cells(CTCs)in breast cancer:a diagnostic tool for prognosis and molecular analysis 被引量:5
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作者 Xiaoshen Dong R.Katherine Alpaugh Massimo Cristofanilli 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2012年第4期388-398,共11页
Metastatic breast cancer (MBC) is characterized by a combination of tumor growth, proliferation and metastatic progression and is typically managed with palliative intent. The benefit of standard systemic therapies ... Metastatic breast cancer (MBC) is characterized by a combination of tumor growth, proliferation and metastatic progression and is typically managed with palliative intent. The benefit of standard systemic therapies is relatively limited and the disease is considered incurable suggesting the need to investigate the biological drivers of the various phases of the metastatic process in order to improve the selection of molecularly driven therapies. The detection, enumeration and molecular analysis of circulating tumor cells (CTCs) provide an intriguing opportunity to advance this knowledge. CTCs enumerated by the Food and Drugs Administration-cleared CellSearchTM system are an independent prognostic factor of progression- free survival (PFS) and overall survival (OS) in MBC patients. Several published papers demonstrated the poor prognosis for MBC patients that presented basal CTC count _〉5 in 7.5 mL of blood. Therefore, the enumeration of CTCs during treatment for MBC provides a tool with the ability to predict progression of disease earlier than standard timing of anatomical assessment using conventional radiological tests. During the metastatic process cancer cells exhibit morphological and phenotypic plasticity undergoing epithelial- mesenchymal transition (EMT). This important phenomenon is associated with down regulation of epithelial marker (e.g., EpCAM) with potential limitations in the applicability of current CTCs enrichment methods. Such observations translated in a number of investigations aimed at improving our capabilities to enumerate and perform molecular characterization of CTCs. Theoretically, the phenotypic analysis of CTCs can represent a "liquid" biopsy of breast tumor that is able to identify a new potential target against the metastatic disease and advance the development and monitoring of personalized therapies. 展开更多
关键词 circulating tumor cells (ctcs metastatic breast cancer (MBC) epithelial-mesenchymal transition(EMT) cancer stem cells
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Circulating tumor cell isolation:the assets of filtration methods with polycarbonate track-etched filters 被引量:5
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作者 Claire Dolfus Nicolas Piton +1 位作者 Emmanuel Toure Jean-Christophe Sabourin 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期479-487,共9页
Circulating tumor cells (CTCs) arise from primary or secondary tumors and enter the bloodstream by active or passive intravasation. Given the low number of CTCs, enrichment is necessary for detection. Filtration met... Circulating tumor cells (CTCs) arise from primary or secondary tumors and enter the bloodstream by active or passive intravasation. Given the low number of CTCs, enrichment is necessary for detection. Filtration methods are based on selection of CTCs by size using a filter with 6.5 to 8 pm pores. After coloration, collected CTCs are evaluated according to morphological criteria. Immunophenotyping and fluorescence in situ hybridization techniques may be used. Selected CTCs can also be cultivated in vitro to provide more material. Analysis of genomic mutations is difficult because it requires adapted techniques due to limited DNA materials. Filtration-selected CTCs have shown prognostic value in many studies but multicentric validating trials are mandatory to strengthen this assessment. Other clinical applications are promising such as follow-up, therapy response prediction and diagnosis. Microfluidic emerging systems could optimize filtration-selected CTCs by increasing selection accuracy. 展开更多
关键词 circulating tumor ceils (ctcs biological markers FILTRATION
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Single-cell analyses of circulating tumor cells 被引量:4
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作者 Xi-Xi Chen Fan Bai 《Cancer Biology & Medicine》 SCIE CAS CSCD 2015年第3期184-192,共9页
Circulating tumor cells(CTCs) are a population of tumor cells mediating metastasis, which results in most of the cancer related deaths. The number of CTCs in the peripheral blood of patients is rare, and many platform... Circulating tumor cells(CTCs) are a population of tumor cells mediating metastasis, which results in most of the cancer related deaths. The number of CTCs in the peripheral blood of patients is rare, and many platforms have been launched for detection and enrichment of CTCs. Enumeration of CTCs has already been used as a prognosis marker predicting the survival rate of cancer patients. Yet CTCs should be more potential. Studies on CTCs at single cell level may help revealing the underlying mechanism of tumorigenesis and metastasis. Though far from developed, this area of study holds much promise in providing new clinical application and deep understanding towards metastasis and cancer development. 展开更多
关键词 circulating tumor cells (ctcs single cell sequencing METASTASIS
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Circulating tumor cells isolation:the "post-EpCAM era" 被引量:4
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作者 Cristina Raimondi Chiara Nicolazzo Angela Gradilone 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期461-470,共10页
Circulating tumor cells (CTCs) represent a submicroscopic fraction detached from a primary tumor and in transit to a secondary site. The prognostic significance of CTCs in metastatic cancer patients was demonstrated... Circulating tumor cells (CTCs) represent a submicroscopic fraction detached from a primary tumor and in transit to a secondary site. The prognostic significance of CTCs in metastatic cancer patients was demonstrated for the first time more than ten years ago. To date, it seems clear enough that CTCs are highly heterogeneous and dynamically change their shape. Thus, the inadequacy of epithelial cell adhesion molecule (EpCAM) as universal marker for CTCs detection seems unquestionable and alternative methods able to recognize a broader spectrum of phenotypes are definitely needed. In this review the pleiotropic functions of EpCAM are discussed in detail and the role of the molecule in the biology of CTCs is critically dissected. 展开更多
关键词 Epithelial cell adhesion molecule(EpCAM) circulating tumor cells(ctcs) epithelial-mesenchymal transition EpICD
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Impact of chronic exposure to bevacizumab on EpCAM-based detection of circulating tumor cells 被引量:2
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作者 Chiara Nicolazzo Isabella Massimi +5 位作者 Lavinia V.Lotti Simone Vespa Cristina Raimondi Fabio Maria Pulcinelli Angela Gradilone Paola Gazzaniga 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期491-496,共6页
Background: Circulating tumor cells (CTCs) are often undetected through the immunomagnetic epithelial cell adhesion molecule (EpCAM)-based CellSearch~ System in breast and colorectal cancer (CRC) patients treat... Background: Circulating tumor cells (CTCs) are often undetected through the immunomagnetic epithelial cell adhesion molecule (EpCAM)-based CellSearch~ System in breast and colorectal cancer (CRC) patients treated with bevacizumab (BEV), where low CTC numbers have been reported even in patients with evidence of progression of disease. To date, the reasons for this discrepancy have not been clarified. This study was carried out to investigate the molecular and phenotypic changes in CRC cells after chronic exposure to BEV in vitro. Methods: The human CRC cell line WiDr was exposed to a clinically relevant dose of BEV for 3 months in vitro. The expression of epithelial and mesenchymal markers and EpCAM isoforms was determined by western blotting and immunofluorescence. To evaluate the impact of EpCAM variant isoforms expression on CTC enumeration by CellSearch, untreated and treated colon cancer cells were spiked into 7.5 mL of blood from a healthy donor and enumerated by CellSearch. Results: Chronic exposure of CRC cell line to BEV induced decreased expression of EpCAM 40 kDa isoform and increased expression EpCAM 42 kDa isoform, together with a decreased expression of cytokeratins (CK), while no evidence of epithelial to mesenchymal transition (EMT) in treated cells was observed. The recovery rate of cells through CellSearch was gradually reduced in course of treatment with BEV, being 84% , 70% and 40% at l, 2 and 3 months, respectively. Conclusions: We hypothesize that BEV may prevent CellSearch from capturing CTCs through altering EpCAM isoforms. 展开更多
关键词 circulating tumor cells (ctcs epithelial cell adhesion molecule (EpCAM) isoform bevacizumab(BEV) colorectal cancer (CRC)
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Growth of Circulating Tumor Cell-Derived Colonies from Peripheral Blood of Melanoma Patients: Preliminary Characterization of Colony Composition 被引量:1
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作者 Wolfram E. Samlowski John R. McGregor +3 位作者 Suzanne T. Samlowski Shweta Tharkar Shirley Shen Joel S. Bentz 《Health》 2014年第12期1467-1481,共15页
Circulating tumor cells (CTC) are rarely detected in the blood of cancer patients, even though they are a direct harbinger of eventual patient demise. We developed an innovative CTC culture technology to allow more se... Circulating tumor cells (CTC) are rarely detected in the blood of cancer patients, even though they are a direct harbinger of eventual patient demise. We developed an innovative CTC culture technology to allow more sensitive isolation, expansion, and characterization of viable colonies from patient blood. In this assay, the entire leukocyte fraction from 10 ml of anticoagulated patient blood is placed into culture medium without any pre-selection. After 16 days in culture, CTC derived colonies are counted. As a proof-of-principle, blood samples from 58 Stage IIa-IV melanoma patients were tested. Ninety percent of these samples grew colonies. The colony numbers ranged from 0 - 308 (mean 63 ± 9.5 SEM). Ten normal volunteers had virtually no growth (mean 0.5 ± 1.4 colonies). Colonies were harvested using a micropipette for characterization. Tumor-cell containing spheroids were embedded in paraffin, sectioned, and stained with melanoma-specific mAb for histologic characterization. MITF proved to be the most consistent immunostain that identified melanoma cells in these colonies. A host-cell component in colonies was also identified using CD68 and CD43 mAb staining. Following enzymatic dissociation of colonies, a variety of immunostains were tested. Papanicolau staining proved most useful for identifying the abnormal nuclei of tumor cells. Flow cytometry could readily distinguish host and tumor cell populations based on DNA content and forward/side scatter in dissociated colonies. The stem cell marker ALDH1A1 associated with the aneuploid population, but CD45 was expressed on both diploid and aneuploid cells. The ability to repeatedly isolate CTC derived colonies from cancer patient blood samples opens the door to a novel type of long-term clinical monitoring. This novel CTC culture technology may prove useful to perform molecular characterization, assessment of treatment response, and testing of drug sensitivity and resistance in patients during treatment. 展开更多
关键词 circulating tumor Cells ctc COLONIES ctc Cultures MELANOMA Flow CYTOMETRY ctc DERIVED COLONIES
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Circulating Tumor Cell Cultures as a Predictive Marker during Salvage Therapy of Refractory Merkel Cell Carcinoma with Chemotherapy and Electron Beam Radiation 被引量:1
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作者 Sreekanth Donepudi Susan A. Reisinger +5 位作者 John R. McGregor Shweta Tharkar Suzanne Samlowski Daniel Ostler Shirley Shen Wolfram E. Samlowski 《Journal of Cancer Therapy》 2013年第7期1162-1166,共5页
Metastatic Merkel Cell carcinoma (MCC) is a highly unusual and aggressive skin cancer that presents as a small, pink to violet skin lesion and metastasizes early in its growth. Metastatic MCC is generally treated with... Metastatic Merkel Cell carcinoma (MCC) is a highly unusual and aggressive skin cancer that presents as a small, pink to violet skin lesion and metastasizes early in its growth. Metastatic MCC is generally treated with small cell lung cancer chemotherapy regimens, because the tumor consists of neuroendocrine cells, but patients generally do not have durable responses. The pathogenesis of MCC has recently been attributed to the Merkel Cell polyoma virus. This virus activates the cellular retinoblastoma oncoprotein and cell cycle machinery, triggering continual cellular proliferation. A 77-year-old man developed extensive MCC metastases, involving more than one fourth of his scalp and numerous cervical lymph nodes. Following failure of initial chemotherapy and radiation, effective palliation was achieved by using a sequence of electron-beam radiotherapy, low dose gemcitabine, and etoposide, resulting in significant periods of tumor regression and prolonged survival. A novel circulating tumor cell (CTC) culture assay was performed on four separate clinic visits during the treatment period. Tumor colonies were cultured from the patient’s peripheral blood and CTC colony counts were correlated with clinical treatment response. Not only did the patient respond to palliative cell cycle directed chemotherapy and electron beam radiation, but we demonstrated that CTC can be cultured from peripheral blood of MCC patients and serve as a predictive marker to monitor treatment response. 展开更多
关键词 MERKEL Cell Carcinoma CHEMOTHERAPY Radiotherapy circulating tumor Cells ctc Culture
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Notes for developing a molecular test for the full characterization of circulating tumor cells
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作者 Elisabetta Rossi Antonella Facchinetti Rita Zamarchi 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期471-478,共8页
The proved association between the circulating tumor cell (CTC) levels and the patients' survival parameters has been growing interest to investigate the molecular profile of these neoplastic cells among which hide... The proved association between the circulating tumor cell (CTC) levels and the patients' survival parameters has been growing interest to investigate the molecular profile of these neoplastic cells among which hide out precursors capable of initiating a new distant metastatic lesion. The full characterization of the tumor cells in peripheral blood of cancer patients is expected to be of help for understanding and (prospectively) for counteracting the metastatic process. The major hitch that is hampering the successful gaining of this result is the lack of a consensus onto standard operating procedures (SOPs) for performing what we generally define as the "liquid biopsy". Here we review the more recent acquisitions in the analysis of CTCs and tumor related nucleic acids, looking to the main open questions that are hampering their definitive employ in the routine clinical practice. 展开更多
关键词 circulating tumor cells (ctcs single-cell analysis liquid biopsy
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Circulating tumor cells in lung cancer:Detection methods and clinical impact
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作者 Minghui Zhang Decai Chi +3 位作者 Shu Zhao Yan Wang Maopeng Yang Yan Wan 《The Chinese-German Journal of Clinical Oncology》 CAS 2014年第10期476-482,共7页
Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinica... Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis. 展开更多
关键词 circulating tumor cells(ctcs lung cancer enrichment method detection method
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Research progress on circulating tumor cell detection in brain gliomas
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作者 Xiaodong Wang Gang Yang 《Journal of Translational Neuroscience》 2021年第1期1-6,共6页
Glioma,the most common primary intracranial tumor,has high morbidity and mortality.The detection of circulating tumor cells(CTCs)is an important part of the liquid biopsy of gliomas.CTCs,carrying the genetic and biolo... Glioma,the most common primary intracranial tumor,has high morbidity and mortality.The detection of circulating tumor cells(CTCs)is an important part of the liquid biopsy of gliomas.CTCs,carrying the genetic and biological information of tumor tissue,provide a new perspective and dimension for the study of tumor metastasis,progression,chemotherapy sensitivity and drug resistance.Cerebrospinal fluid(CSF)circulates through the ventricle and spinal cord cistern,which can better maintain the original information of tumor cells compared with the complicated environments of tissues and plasma.Study on the dynamic changes of CTCs in the CSF of the central nervous system(CNS)is relatively rare.However,the analysis of CTCs in CSF can be used to guide the treatment of gliomas and reveal the patho-physiological and genetic mechanisms of tumor cell metastasis to the CSF.This paper reviews the progress in the research on CTC detection in gliomas. 展开更多
关键词 glioma liquid biopsy circulating tumor cells(ctcs) cerebrospinal fluid(CSF) central nervous system(CNS) computed tomography(CT) positron emission computed tomography(PET-CT) magnetic resonance imaging(MRI) circulating tumor DNA(ctDNA) extracellular vesicles(EV) epithelial surface tumor marker(EpCAM) epithelial mesenchymal transformation(EMT)
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免疫磁珠负性富集结合免疫荧光抗体技术检测卵巢癌患者外周血CTCs的方法建立和应用 被引量:8
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作者 赵枰 洪煜婧 +2 位作者 陈相 鲁晓燕 洪宏 《现代检验医学杂志》 CAS 2017年第3期56-58,62,共4页
目的建立基于细胞学水平的循环肿瘤细胞负性富集技术来检测卵巢上皮性癌(卵巢癌)患者外周血循环肿瘤细胞。方法体外培养卵巢癌SKOV-3细胞,按一定比例掺入到2 ml健康人外周静脉血中,应用免疫磁珠为基础的负性富集结合免疫荧光抗体技术对... 目的建立基于细胞学水平的循环肿瘤细胞负性富集技术来检测卵巢上皮性癌(卵巢癌)患者外周血循环肿瘤细胞。方法体外培养卵巢癌SKOV-3细胞,按一定比例掺入到2 ml健康人外周静脉血中,应用免疫磁珠为基础的负性富集结合免疫荧光抗体技术对循环肿瘤细胞进行定量检测。评价该方法富集靶细胞的回收率,并用上述方法检测32例卵巢癌患者和10例对照者外周血中的循环肿瘤细胞。结果①在健康志愿者2 ml外周血掺入不同数目SKOV-3细胞的条件下,肿瘤细胞的回收率在64%~80%之间,掺入细胞间回归方程为Y=0.782X-1.408,相关系数为0.998。②10例对照者外周血中未检测到CK8/18+,18例卵巢癌患者的外周血中检测到CK8/18+,阳性率为56.3%(18/32,X^2=7.681,P<0.01)。③CTCs阳性与远处转移密切相关(X^2=5.776,P<0.05)。结论免疫磁珠负性富集结合免疫荧光抗体技术检测卵巢癌患者外周血循环肿瘤细胞的方法可能具有一定的临床应用价值。 展开更多
关键词 循环肿瘤细胞 卵巢上皮性癌 负性富集技术 免疫荧光抗体技术
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立体定向精准放射治疗对老年非小细胞肺癌的临床疗效及对外周血CTCs和TK1的影响 被引量:5
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作者 高玉伟 王杰 赵丽娟 《中国医学装备》 2021年第10期110-115,共6页
目的:探讨立体定向精准放射治疗(SBRT)对老年非小细胞肺癌(NSCLC)的临床疗效及对外周血循环肿瘤细胞(CTCs)和胸苷激酶1(TK1)的影响。方法:选取医院收治的60例NSCLC患者,依据随机数表法将其分为观察组和对照组,每组30例。观察组采用SBRT... 目的:探讨立体定向精准放射治疗(SBRT)对老年非小细胞肺癌(NSCLC)的临床疗效及对外周血循环肿瘤细胞(CTCs)和胸苷激酶1(TK1)的影响。方法:选取医院收治的60例NSCLC患者,依据随机数表法将其分为观察组和对照组,每组30例。观察组采用SBRT,对照组采用常规放射治疗,记录治疗期间两组不良反应发生情况,随访3年记录生存期。评估治疗前后肺功能,以及治疗前后、治疗后1年和末次随访检测外周血CTCs数目和TK1水平。结果:观察组治疗总有效率和疾病控制率分别为63.3%和86.7%,显著高于对照组的36.7%和63.3%,差异有统计学意义(x^(2)=4.267,x^(2)=4.356;P<0.05)。治疗后,观察组肺通气阻抗中的中心阻力(Rc)、周边阻力(Rp)、5 Hz的总气道阻力(R5)、20 Hz的总气道阻力(R20)和呼吸总阻抗(Zrs)指标均低于对照组,差异有统计学意义(t=4.076,t=3.429,t=3.942,t=3.083,t=3.545;P<0.05);观察组不良反应中恶心呕吐、食欲下降、皮肤瘙痒和湿疹均低于对照组,差异有统计学意义(x^(2)=4.800,x^(2)=4.444,x^(2)=4.022,x^(2)=4.356;P<0.05);观察组放射性肺炎、放射性食管炎发生率低于对照组,差异有统计学意义(x^(2)=19.631,x^(2)=10.814;P<0.05);观察组各时间点外周血CTCs数目、TK1水平均低于对照组,差异有统计学意义(F=19.834,F=15.023;P<0.05)。观察组中位生存期显著高于对照组,差异有统计学意义(x^(2)=21.280,P<0.05)。结论:SBRT对老年NSCLC安全性高,对肺损伤小,不良反应少,并能降低外周血CTCs数目和TK1水平,改善肺功能,提高近期疗效,延长生存期。 展开更多
关键词 立体定向精准放射治疗(SBRT) 非小细胞肺癌(NSCLC) 肺功能 循环肿瘤细胞(ctcs) 胸苷激酶1(TK1)
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血清VEGF、TLR4水平及CTCs在肺癌、乳腺癌和前列腺癌骨转移中的临床意义 被引量:13
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作者 宁尚福 杨勇 +1 位作者 凌振雄 林才华 《海南医学》 CAS 2021年第19期2469-2472,共4页
目的探讨血管调控因子血管内皮生长因子(VEGF)、Toll样受体4 (TLR4)联合循环肿瘤细胞(CTCs)在肺癌、乳腺癌和前列腺癌骨转移中的临床意义。方法回顾性分析吴川市人民医院肿瘤科2019年1~12月收治的100例初诊肺癌、乳腺癌及前列腺癌骨转... 目的探讨血管调控因子血管内皮生长因子(VEGF)、Toll样受体4 (TLR4)联合循环肿瘤细胞(CTCs)在肺癌、乳腺癌和前列腺癌骨转移中的临床意义。方法回顾性分析吴川市人民医院肿瘤科2019年1~12月收治的100例初诊肺癌、乳腺癌及前列腺癌骨转移患者(骨转移组)的临床资料,观察不同骨转移灶数目、临床疗效及有无骨相关发生事件发生患者的VEGF、TLR4、CTCs表达水平;另选择同期我院收治的94例初诊肺癌、乳腺癌及前列腺癌无骨转移患者纳入对照组,比较骨转移组与对照组患者的VEGF、TLR4、CTCs表达水平。结果骨转移组患者的VEGF、TLR4、CTCs阳性率分别为(341.85±40.68) pg/mL、14.19±1.37、67.00%,明显高于对照组的(236.97±28.75) pg/mL、10.15±1.12、14.89%,差异均具有统计学意义(P<0.05);骨转移灶数目≥3处组患者的VEGF、TLR4、CTCs阳性率分别为(355.09±43.29) pg/mL、15.03±1.48、82.35%,明显高于骨转移灶数目<3处组的313.72±1.33、62.03%,明显低于无效组的(371.14±44.27) pg/mL、16.28±1.53、85.71%,差异均具有统计学意义(P<0.05);有骨相关事件发生组患者的VEGF、TLR4、CTCs阳性率分别为(363.03±42.26) pg/mL、15.79±1.41、79.49%,明显高于无骨相关事件发生组的(328.31±39.67) pg/mL、13.17±1.34、59.02%,差异均有统计学意义(P<0.05)。结论血管调控因子VEGF、TLR4及CTCs可用于肺癌、乳腺癌及前列腺癌骨转移的诊断、疗效和骨相关事件的评估。 展开更多
关键词 肺癌 乳腺癌 前列腺癌 骨转移 血管内皮生长因子 Toll样受体4 循环肿瘤细胞
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Circulating cancer stem cells:the importance to select 被引量:10
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作者 Ming-Hsin Yang Ahmet Imrali Christopher Heeschen 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期437-449,共13页
It has been demonstrated that even localized tumors withottt chnically apparent metastasis give rise to circulating tumor cells (CTCs). A growing number of technically diverse platforms are being developed for detec... It has been demonstrated that even localized tumors withottt chnically apparent metastasis give rise to circulating tumor cells (CTCs). A growing number of technically diverse platforms are being developed for detecting/isolating CTCs in the circulating blood. Despite the technical challenges of isolating rare CTCs from blood, recent studies have already shown the predictive value of CTCs enumeration. Thus, it is becoming increasingly accepted that CTC numbers are linked to patients' outcome and may also be used to monitor treatment response and disease relapse, respectively. Further CTCs provide a non-invasive source for tumor material, 'liquid biopsy', which is particularly important for patients, where no biopsy material can be obtained or where serial biopsies of the tumor, e.g., following treatment, are practically impossible. On the other hand the molecular and biological characterization of CTCs has still remained at a rather experimental stage. Future studies are necessary to define CTC heterogeneity to establish the crucial role of circulating cancer stem cells for driving metastasis, which represent a distinct subpopulation of CTCs that bear metastasis-initiating capabilities based on their sternness properties and invasiveness and thus are critical for the patients' clinical outcome. As compared to non-tumorigenic/metastatic bulk CTCs, circulating cancer stem cells may not only be capable of evading from the primary tumor, but also escape from immune surveillance, survive in the circulating blood and subsequently form metastases in distant organs. Thus, circulating cancer stem cells represent a subset of exclusively tumorigenic cancer stem cells characterized by their invasive characteristics and are potential therapeutic targets for preventing disease progression. To date, only a few original reports and reviews have been published focusing on circulating cancer stem cells. This review discusses the potential importance of isolating and characterizing these circulating cancer stem cells, but also highlights current technological limitations. 展开更多
关键词 Cancer stem cells circulating tumor cells (ctcs drug resistance
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Interaction between circulating cancer cells and platelets:clinical implication 被引量:3
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作者 Xiao-Liang Lou Jian Sun +3 位作者 Shu-Qi Gong Xue-Feng Yu Rui Gong Huan Deng 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期450-460,共11页
Metastasis is the main cause of cancer-associated mortality. During this complicated process, some cancer cells, also called circulating tumor cells (CTCs), detach from primary sites, enter bloodstream and extravasa... Metastasis is the main cause of cancer-associated mortality. During this complicated process, some cancer cells, also called circulating tumor cells (CTCs), detach from primary sites, enter bloodstream and extravasate at metastatic site. Thrombocytosis is frequently observed in patients with metastatic cancers suggesting the important role of platelets in metastasis. Therefore this review focuses on how platelets facilitate the generation of CTCs, protect them from various host attacks, such as immune assaults, apoptosis and shear stress, and regulate CTCs intravasation/extravasation. Platelet-derived cytokines and receptors are involved in this cascade. Identification the mechanisms underlie platelet-CTCs interactions could lead to the development of new platelet-targeted therapeutic strategy to reduce metastasis. 展开更多
关键词 circulating tumor cells (ctcs PLATELET epithelial-mesenchymal transition (EMT) immunesurveillance METASTASIS
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乳腺癌患者CTCs、ctDNA检测及其诊断价值研究 被引量:3
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作者 季福庆 李梦婓 +1 位作者 印玉龙 吕勇刚 《海南医学》 CAS 2020年第7期838-841,共4页
目的探讨乳腺癌患者的循环肿瘤细胞(CTCs)、循环肿瘤细胞DNA (ctDNA)检测水平及其诊断价值,为患者的临床诊断提供指导。方法选取2017年1月至2019年1月西北大学附属医院收治的60例乳腺癌患者作为观察组,同期体检健康者50例作为对照组。... 目的探讨乳腺癌患者的循环肿瘤细胞(CTCs)、循环肿瘤细胞DNA (ctDNA)检测水平及其诊断价值,为患者的临床诊断提供指导。方法选取2017年1月至2019年1月西北大学附属医院收治的60例乳腺癌患者作为观察组,同期体检健康者50例作为对照组。采集两组受检者的外周血实施CTCs及ctDNA检测,比较观察组不同特征患者及对照组的CTCs阳性率与ctDNA水平。结果观察组患者的CTCs阳性率为38.33%(23/60),ctDNA水平为(152.6±12.4) ng/mL;对照组CTCs均为阴性,ctDNA水平为(28.1±4.3) ng/mL,观察组CTCs阳性率与ctDNA水平明显高于对照组,差异均有统计学意义(P<0.05);不同年龄与病理类型乳腺癌患者的CTCs阳性率与ctDNA水平比较差异均无统计学意义(P>0.05);TNM分期Ⅰ期患者的CTCs阳性率为0,ctDNA水平为(110.2±8.6) ng/mL,TNM分期Ⅱ期患者的CTCs阳性率为25.00%,ctDNA水平为(143.6±10.2) ng/mL,TNM分期Ⅲ期患者的CTCs阳性率为51.43%,ctDNA水平为(168.9±8.5) ng/mL,不同乳腺癌TNM分期患者CTCs阳性率与ctDNA水平比较差异均有统计学意义(P<0.05);M0期患者的CTCs阳性率为12.50%,ctDNA水平为(125.8±8.9) ng/mL,M1期患者的CTCs阳性率为90.00%,ctDNA水平为(176.5±10.8) ng/mL,不同乳腺癌M分期患者CTCs阳性率与ctDNA水平比较差异均具有统计学意义(P<0.05)。结论乳腺癌患者CTCs、ctDNA检测可为患者的临床诊断提供指导。 展开更多
关键词 乳腺癌 循环肿瘤细胞 循环肿瘤细胞DNA 诊断价值
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检测循环肿瘤细胞(CTC)在临床肿瘤诊断和治疗中的作用 被引量:8
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作者 周小昀 李龙芸 《癌症进展》 2006年第3期240-243,195,共5页
有关循环肿瘤细胞的理论已有很长时间。肿瘤转移及复发的过程均是一个循环肿瘤细胞定植生长的级连放大的瀑布反应。目前肿瘤的发现和诊断仍高度依赖于影像学及肿瘤特异性血清标志物的方法,不能及时发现肿瘤的转移和复发。基于肿瘤细胞... 有关循环肿瘤细胞的理论已有很长时间。肿瘤转移及复发的过程均是一个循环肿瘤细胞定植生长的级连放大的瀑布反应。目前肿瘤的发现和诊断仍高度依赖于影像学及肿瘤特异性血清标志物的方法,不能及时发现肿瘤的转移和复发。基于肿瘤细胞播散理论,检测循环肿瘤细胞可更早期有效发现肿瘤的早期转移和复发。本文对文献报道的检测循环肿瘤细胞在临床肿瘤诊断和治疗中的作用进行了总结,并与目前经典的标志物和影像学进行了比较。 展开更多
关键词 循环肿瘤细胞(ctc) 实体瘤
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循环肿瘤细胞联合血清整合素β1对病理性乳头溢液患者乳管内恶性病变的诊断价值
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作者 张腾 张超 +4 位作者 李智 于满 任欣玲 田梓儒 史立晖 《中国临床新医学》 2024年第5期543-547,共5页
目的探讨循环肿瘤细胞(CTCs)联合整合素β1(ITGβ1)对病理性乳头溢液(PND)患者乳管内恶性病变的诊断价值。方法回顾性分析2022年1月至2023年9月北京市通州区妇幼保健院及首都医科大学附属北京朝阳医院收治的212例PND患者的临床资料,根... 目的探讨循环肿瘤细胞(CTCs)联合整合素β1(ITGβ1)对病理性乳头溢液(PND)患者乳管内恶性病变的诊断价值。方法回顾性分析2022年1月至2023年9月北京市通州区妇幼保健院及首都医科大学附属北京朝阳医院收治的212例PND患者的临床资料,根据乳管病理检查结果将研究对象分为良性病变组(187例)和恶性病变组(25例)。采用差异富集-荧光免疫染色原位杂交技术(SE-iFISH)检测PND患者CTCs数量,采用酶联免疫吸附试验检测PND患者血清ITGβ1水平。采用多因素logistic回归分析PND患者发生乳管内恶性病变的影响因素。通过受试者工作特征(ROC)曲线分析CTCs数量、血清ITGβ1水平对PND患者乳管内恶性病变的诊断效能。结果两组年龄、体质量指数(BMI)、月经状态、溢液类型、乳腺肿块比较差异有统计学意义(P<0.05)。恶性病变组CTCs数量多于良性病变组,血清ITGβ1水平高于良性病变组,差异有统计学意义(P<0.05)。多因素logistic回归分析结果显示,年龄>50岁、BMI>24 kg/m^(2)、血样溢液、较多的CTCs数量、较高的血清ITGβ1水平是PND患者发生乳管内恶性病变的独立危险因素(P<0.05)。CTCs数量、血清ITGβ1水平均具有诊断PND患者乳管内恶性病变的应用价值(P<0.05),且两项指标联合检测的诊断效能更优[AUC(95%CI)=0.961(0.925~0.983),P<0.001],灵敏度和特异度分别为96.00%、84.49%。结论CTCs数量、血清ITGβ1水平在乳管内恶性病变的PND患者体内显著升高,二者联合对PND患者乳管内恶性病变具有较好的诊断价值。 展开更多
关键词 病理性乳头溢液 乳管内恶性病变 循环肿瘤细胞 整合素Β1 诊断价值
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Envita’s Precision Cancer Care: 35-Fold Improvement in Response Rates
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作者 Sheba Goklany John C. Oertle III +19 位作者 Ronald Matthias Jr. Daniel Warren David Medina Rory Sears Robert Zieve Kendra Quart Christopher Aussems Jon Moma Shannon Miller Zach Poteet Conner Coffin Courtney Middleton Erika Ware Phylicia Zarnosky Julie Nowak Winlove Suasin Daniel Conway Chad Burk Ruth Tan-Lim Dino Prato 《Journal of Cancer Therapy》 2024年第4期99-120,共22页
New clinical approaches are imperative beyond the widely adopted National Comprehensive Cancer Network (NCCN) guidelines, utilized by prominent cancer institutions. Cancer is the leading cause of death among individua... New clinical approaches are imperative beyond the widely adopted National Comprehensive Cancer Network (NCCN) guidelines, utilized by prominent cancer institutions. Cancer is the leading cause of death among individuals younger than 85 years within the United States. Despite significant technological advances, including the expenditure of hundreds of billions, treatment outcomes and overall survival have not notably improved for most types of advanced cancer over the last several decades. Over the past 24 years, Envita Medical Centers has pioneered a unique form of personalized treatment approach for late-stage and refractory cancer patients, introducing groundbreaking innovations in the field. Our integrated algorithm utilizes advanced genomics, transcriptomics, and highly tailored immunotherapy, resulting in remarkable outcome improvements. This study presents Envita’s innovative personalized treatment algorithms and examines the response outcomes of 199 late-stage cancer patients treated at Envita Medical Centers over a two-year period. Compared to standard of care and palliative chemotherapy, Envita’s treatment demonstrated a remarkable 35-fold improvement in overall response rates (Figure 1). Moreover, 88% of the patients, the majority presenting with Stage 3 or 4 cancer, experienced a 43-fold improvement in quality of life with minimal side effects, as compared to standard of care chemotherapy and palliative care. This revolutionary success is attributed to Envita’s personalized therapeutic algorithms, which incorporate customized immunotherapy. Envita’s precision care approach has also achieved a 100% better response rate compared to over 65 global chemotherapy clinical trials with more than 2700 patients. The results from this study suggest that a wider utilization of Envita’s personalized approach can significantly benefit patients with late-stage and refractory cancer. 展开更多
关键词 Envita Medical Centers Late-stage Cancer Overall Response Rate Quality of Life circulating tumor Cells (ctcs) Mutant Allele Frequency (MAF) Precision Care
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非小细胞肺癌循环肿瘤细胞的检测和临床意义的研究
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作者 周碧兰 易宏辉 余艳宏 《贵州医药》 CAS 2024年第11期1691-1693,1698,共4页
目的对非小细胞肺癌(NSCLC)患者中循环肿瘤细胞(CTCs)的表达情况进行检测,并探讨其与临床病理特征、肿瘤复发转移的关系。方法采集37例NSCLC患者和10例健康对照者外周血后,通过CanPatrol TM CTCs富集技术和多重RNA原位分析技术对外周血... 目的对非小细胞肺癌(NSCLC)患者中循环肿瘤细胞(CTCs)的表达情况进行检测,并探讨其与临床病理特征、肿瘤复发转移的关系。方法采集37例NSCLC患者和10例健康对照者外周血后,通过CanPatrol TM CTCs富集技术和多重RNA原位分析技术对外周血中的CTCs进行分离和检测,并探讨NSCLC患者在手术前的临床病理参数和CTCs阳性率之间的关系,进一步分析NSCLC患者术后的CTCs表达情况与肿瘤复发转移的关系。结果健康者的外周血中未检出CTCs,而NSCLC患者的CTCs阳性率高达75.7%(28/37),并且CTCs的阳性率随NSCLC患者临床分期的增加而呈现一定的上升趋势。CTCs表达情况与患者自身的性别、年龄、分化程度无显著相关性,但与病理亚型有相关性,腺癌型NSCLC患者中的CTCs低于鳞癌型NSCLC患者。32例复查患者中有13例患者出现了复发转移,其中复发转移与未复发转移患者的CTCs阳性率分别为92.3%(12/13)和57.9%(11/19),该差异具有明显的统计学意义(P<0.05)。结论检测NSCLC患者中CTCs的表达具有一定的临床参考意义,有望成为NSCLC诊断及预后评估的辅助手段。 展开更多
关键词 循环肿瘤细胞(ctcs) 非小细胞肺癌(NSCLC) 阳性率
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