为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,...为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.展开更多
The series of biradicals with m phenylene coupling unit and hetero spin centers were calculated compared with those possessing homo spin centers using AM1 CI method. A simple rule was proposed to design high s...The series of biradicals with m phenylene coupling unit and hetero spin centers were calculated compared with those possessing homo spin centers using AM1 CI method. A simple rule was proposed to design high spin molecules with ferromagnetic coupling unit and hetero spin centers. Two neutral (or charged) hetero spin centers resulted in high spin ground state, one neutral and another charged hetero spin centers correspond to low spin ground state. The latter was ascribed to the huge splitting of two partially occupied molecular orbitals.展开更多
Environmentally-friendly magnetic metallic absorbers with high-performing antioxidant property,thermal stability,and anticorrosion capability have attracted great attention in real-world applications.A surface modific...Environmentally-friendly magnetic metallic absorbers with high-performing antioxidant property,thermal stability,and anticorrosion capability have attracted great attention in real-world applications.A surface modification technology of magnetic metallic absorbers with dense and inert materials has been an effective strategy to solve the aforesaid problem.Herein,fluorinefree core–shell carbonyl iron-organic silicon absorbers(CI@SiO_(2)/1,1,1,3,3,3-hexamethyl disilazane(HMDS))were fabricated via a facile one-pot synthesis using tetraethyl orthosilicate(TEOS)and HMDS as the precursor of protective layer(SiO_(2)/HMDS),and CI@SiO_(2)/HMDS hybrid reveals its long-term corrosion resistance and excellent microwave absorption performance with a minimum reflection loss value of−44.3 dB and an effective absorption bandwidth of 5.3 GHz at a thin thickness of 2.0 mm after immersion in 5.0 wt.%NaCl acidic solutions for 2,160 h.Meanwhile,CI@SiO_(2)/HMDS hybrid can still achieve the maximum radar cross-sectional(RCS)reduction values about 16.5 dB·m^(2) at the detectionθof 0°.The exceptional microwave absorption performance and structural stability are largely due to the extraordinary wave-transparent property and shielding ability against corrosive medium of SiO_(2)/HMDS hydrophobic protective layer with a contact angle of 132.5°.The research paves the way for the large-scale and batch production of high-performance magnetic metallic absorbers and increases their survivability and reliability in the harsh environments.展开更多
文摘为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.
文摘The series of biradicals with m phenylene coupling unit and hetero spin centers were calculated compared with those possessing homo spin centers using AM1 CI method. A simple rule was proposed to design high spin molecules with ferromagnetic coupling unit and hetero spin centers. Two neutral (or charged) hetero spin centers resulted in high spin ground state, one neutral and another charged hetero spin centers correspond to low spin ground state. The latter was ascribed to the huge splitting of two partially occupied molecular orbitals.
基金supported by the National Natural Science Foundation of China(Nos.51972045 and 5197021414)the Fundamental Research Funds for the Chinese Central Universities,China(No.ZYGX2019J025)+2 种基金Sichuan Science and Technology Program(Nos.2020JDRC0015 and 2020JDRC0045)the Natural Science Foundation of Sichuan(No.2022NSFSC0347)Sichuan Science and Technology Innovation Talent Project(No.2021JDRC0021).
文摘Environmentally-friendly magnetic metallic absorbers with high-performing antioxidant property,thermal stability,and anticorrosion capability have attracted great attention in real-world applications.A surface modification technology of magnetic metallic absorbers with dense and inert materials has been an effective strategy to solve the aforesaid problem.Herein,fluorinefree core–shell carbonyl iron-organic silicon absorbers(CI@SiO_(2)/1,1,1,3,3,3-hexamethyl disilazane(HMDS))were fabricated via a facile one-pot synthesis using tetraethyl orthosilicate(TEOS)and HMDS as the precursor of protective layer(SiO_(2)/HMDS),and CI@SiO_(2)/HMDS hybrid reveals its long-term corrosion resistance and excellent microwave absorption performance with a minimum reflection loss value of−44.3 dB and an effective absorption bandwidth of 5.3 GHz at a thin thickness of 2.0 mm after immersion in 5.0 wt.%NaCl acidic solutions for 2,160 h.Meanwhile,CI@SiO_(2)/HMDS hybrid can still achieve the maximum radar cross-sectional(RCS)reduction values about 16.5 dB·m^(2) at the detectionθof 0°.The exceptional microwave absorption performance and structural stability are largely due to the extraordinary wave-transparent property and shielding ability against corrosive medium of SiO_(2)/HMDS hydrophobic protective layer with a contact angle of 132.5°.The research paves the way for the large-scale and batch production of high-performance magnetic metallic absorbers and increases their survivability and reliability in the harsh environments.
