Non-coding transcription is present in all eukaryotic genomes,but we lack fundamental knowledge about its importance for an organism’s ability to develop properly.In plants,emerging evidence highlights the essential ...Non-coding transcription is present in all eukaryotic genomes,but we lack fundamental knowledge about its importance for an organism’s ability to develop properly.In plants,emerging evidence highlights the essential biological role of non-coding transcription in the regulation of coding transcription.However,we have few molecular insights into this regulation.Here,we show that a long isoform of the long noncoding RNA SVALKA-L(SVK-L)forms a natural antisense transcript to the host gene CBF1 and negatively regulates CBF1 mRNA levels at normal temperatures in the model plant Arabidopsis thaliana.Furthermore,we show detailed evidence for the specific mode of action of SVK-L.This pathway includes the formation of double-stranded RNA that is recognized by the DICER proteins and subsequent downregulation of CBF1 mRNA levels.Thus,the CBF1-SVK regulatory circuit is not only important for its previously known role in cold temperature acclimation but also for biomass production at normal temperatures.Our study characterizes the developmental role of SVK-L and offers mechanistic insight into how biologically important overlapping natural antisense transcripts can act on and fine-tune the steady-state levels of their host gene’s mRNA.展开更多
基金funded by the Swedish Research Council(2018-03926)Carl Trygger Foundation(20:224).
文摘Non-coding transcription is present in all eukaryotic genomes,but we lack fundamental knowledge about its importance for an organism’s ability to develop properly.In plants,emerging evidence highlights the essential biological role of non-coding transcription in the regulation of coding transcription.However,we have few molecular insights into this regulation.Here,we show that a long isoform of the long noncoding RNA SVALKA-L(SVK-L)forms a natural antisense transcript to the host gene CBF1 and negatively regulates CBF1 mRNA levels at normal temperatures in the model plant Arabidopsis thaliana.Furthermore,we show detailed evidence for the specific mode of action of SVK-L.This pathway includes the formation of double-stranded RNA that is recognized by the DICER proteins and subsequent downregulation of CBF1 mRNA levels.Thus,the CBF1-SVK regulatory circuit is not only important for its previously known role in cold temperature acclimation but also for biomass production at normal temperatures.Our study characterizes the developmental role of SVK-L and offers mechanistic insight into how biologically important overlapping natural antisense transcripts can act on and fine-tune the steady-state levels of their host gene’s mRNA.
