The objective of this study was to evaluate the inclusion of increasing levels of citrus pulp in horse diets by investigating nutrient digestibility and the diet carbohydrate fractions, as well as to evaluate the corr...The objective of this study was to evaluate the inclusion of increasing levels of citrus pulp in horse diets by investigating nutrient digestibility and the diet carbohydrate fractions, as well as to evaluate the correlation observed between the studied digestibility coefficients. Five adult horses were used. The horses were housed in individual stalls and had approximate ages of 3.5 years and live weights of 492.5 ± 44.5 kg. The experimental diets were formulated to meet the animals’ requirements for maintenance, establishing a roughage-to-concentrate ratio of 60:40 with the coast-cross hay as the roughage. The formulated concentrates contained increasing inclusion levels of citrus pulp (0%, 7%, 14%, 21%, and 28%). No effect of the diets was observed (p> 0.05) on the coefficients of digestibility of dry matter, organic matter, crude protein, ether extract, nitrogen-free extract, and non-fibrous carbohydrates;however, there was an effect (p< 0.05) on the soluble carbohydrates,y= 66.298 + 0.3724x. Citrus pulp may be used in horse diets at up to 28% concentrate. Citrus pulp is a safe energy source and benefits the digestibility of the nutrients and the carbohydrate fraction (both the fibrous and non-fibrous fractions) of the diet.展开更多
A cDNA library was constructed end characterized from the pulp of Cera Care navel orange （Citrus sinensis Osbeck） at different stages of ripening. Tittering results revealed that approximately 5.086×10^5 indepe...A cDNA library was constructed end characterized from the pulp of Cera Care navel orange （Citrus sinensis Osbeck） at different stages of ripening. Tittering results revealed that approximately 5.086×10^5 independent clones were included in this library. Electrophoresls gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified end the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBenk, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type Ⅲ metallothlonein-llke （MT） gene was observed to occur 13 tlmes, Indlcatlng that the protein may play an important role In frult development and rlpenlng.展开更多
基金the support of the Faculty of Animal Science and Food Engineering,University of Sao Paulo and the financial support of the Coordination of Improvement of Higher Education Personnel(CAPES).
文摘The objective of this study was to evaluate the inclusion of increasing levels of citrus pulp in horse diets by investigating nutrient digestibility and the diet carbohydrate fractions, as well as to evaluate the correlation observed between the studied digestibility coefficients. Five adult horses were used. The horses were housed in individual stalls and had approximate ages of 3.5 years and live weights of 492.5 ± 44.5 kg. The experimental diets were formulated to meet the animals’ requirements for maintenance, establishing a roughage-to-concentrate ratio of 60:40 with the coast-cross hay as the roughage. The formulated concentrates contained increasing inclusion levels of citrus pulp (0%, 7%, 14%, 21%, and 28%). No effect of the diets was observed (p> 0.05) on the coefficients of digestibility of dry matter, organic matter, crude protein, ether extract, nitrogen-free extract, and non-fibrous carbohydrates;however, there was an effect (p< 0.05) on the soluble carbohydrates,y= 66.298 + 0.3724x. Citrus pulp may be used in horse diets at up to 28% concentrate. Citrus pulp is a safe energy source and benefits the digestibility of the nutrients and the carbohydrate fraction (both the fibrous and non-fibrous fractions) of the diet.
基金Supported by the National Natural Science Foundation of China （30123001, 30300241, and 30471201）, the High-Tech Research and Development （863） Program of China, and Hubei Provincial Natural Science Foundation of China （2003ABA107）.
文摘A cDNA library was constructed end characterized from the pulp of Cera Care navel orange （Citrus sinensis Osbeck） at different stages of ripening. Tittering results revealed that approximately 5.086×10^5 independent clones were included in this library. Electrophoresls gel results of 15 randomly selected clones revealed that the size of the insertion fragments ranged from 400 bp to 2 kb, with an average size of 900 bp. Sequencing results of 150 randomly picked clones showed that the recombination rate was 94%. During subsequent sequence analysis, 41 of 139 clones failed to be identified end the amino sequence of 71 clones shared less than 30% identity with related plants in GenBank. Of 27 clones whose amino sequences shared more than 60% identity with other related plants in GenBenk, 17 clones showed an 80% identity with the corresponding candidate genes of citrus. The clone recognized as the type Ⅲ metallothlonein-llke （MT） gene was observed to occur 13 tlmes, Indlcatlng that the protein may play an important role In frult development and rlpenlng.
