BACKGROUND The prevalence of nonalcoholic fatty liver disease(NAFLD)in patients with chronic hepatitis B(CHB)has increased in recent clinical practice;however,the relationship between CHB and hepatic steatosis(HS)rema...BACKGROUND The prevalence of nonalcoholic fatty liver disease(NAFLD)in patients with chronic hepatitis B(CHB)has increased in recent clinical practice;however,the relationship between CHB and hepatic steatosis(HS)remains controversial.AIM To shed light on the potential association between NAFLD and hepatitis B virus(HBV)infection.METHODS We conducted a systematic literature search using multiple databases,including PubMed,the Cochrane Library,Web of Science,and EMBASE,to identify relevant studies.Predefined inclusion criteria were used to determine the eligibility of the studies for further analysis.RESULTS Comprehensive meta-analysis software was used for statistical analysis,which covered 20 studies.The results indicated a lower NAFLD susceptibility in HBVinfected individuals(pooled OR=0.87;95%CI=0.69-1.08;I2=91.1%),with diabetes(P=0.015),body mass index(BMI;P=0.010),and possibly age(P=0.061)as heterogeneity sources.Of note,in four studies(6197 HBV patients),HBV-infected individuals had a reduced NAFLD risk(OR=0.68,95%CI=0.51-0.89,P=0.006).A positive link between hyperlipidemia and metabolic syndrome emerged in hepatitis B patients,along with specific biochemical indicators,including BMI,creatinine,uric acid,fasting blood glucose,and homeostasis model assessment of insulin resistance.CONCLUSION HBV infection may provide protection against HS;however,the occurrence of HS in patients with HBV infection is associated with metabolic syndrome and specific biochemical parameters.展开更多
BACKGROUND:Non-alcoholic fatty liver disease(NAFLD)is one of the most frequent causes of liver diseases,with markedly increased prevalence.However,its mechanisms are not clear.The present study was undertaken to illus...BACKGROUND:Non-alcoholic fatty liver disease(NAFLD)is one of the most frequent causes of liver diseases,with markedly increased prevalence.However,its mechanisms are not clear.The present study was undertaken to illustrate the role of caveolin-1(cav1)and the scavenger receptor class B type 1(SR-B1)in NAFLD.METHODS:Adult male C57BL/6 mice were fed with a normal diet or high fat and cholesterol(HFC)diet for 14 weeks.The mice were sacrificed to collect plasma and harvest the liver;their plasma lipid concentration was measured.Hepatic cav1and SR-B1 mRNA and protein expression were determined by real-time quantitative polymerase chain reaction(qPCR)and Western blotting,respectively.In order to study cav1 and SR-B1distribution and change in hepatocytes,immunohistochemical analysis was performed.RESULTS:HFC diet increased plasma lipids,induced NAFLD and increased the liver/body weight ratio.Compared to the control mice(n=6),the mRNA and protein levels of cav1 and SR-B1 in liver tissue of the NAFLD mice(n=12)increased significantly(cav1 mRNA:1.536±0.226 vs 0.980±0.272,P【0.05;protein:0.643±0.240 vs 0.100±0.130,P【0.01;SR-B1 mRNA:1.377±0.125 vs 0.956±0.151,P【0.01;protein:2.156±0.507vs 0.211±0.211,P【0.01).Furthermore,both cav1 and SR-B1immunoreactivity increased and their distribution was also changed,mainly in the plasma membrane of hepatocytes,cytoplasm and membrane of lipid droplets and around.CONCLUSION:NAFLD is associated with increased concentration of plasma lipids and upregulation of hepatic cav1 and SR-B1 gene and protein expressions,which indicate that cav1 and SR-B1 might play crucial roles in the pathogenesis of NAFLD.展开更多
Atherosclerosis is a leading underlying factor in cardiovascular disease and stroke,important causes of morbidity and mortality across the globe.Abundant epidemiological studies demonstrate that high levels of high de...Atherosclerosis is a leading underlying factor in cardiovascular disease and stroke,important causes of morbidity and mortality across the globe.Abundant epidemiological studies demonstrate that high levels of high density lipoprotein（HDL） are associated with reduced risk of atherosclerosis and preclinical,animal model studies demonstrate that this association is causative.Understanding the molecular mechanisms underlying the protective effects of HDL will allow more strategic approaches to development of HDL based therapeutics.Recent evidence suggests that an important aspect of the ability of HDL to protect against atherosclerosis is its ability to trigger signaling responses in a variety of target cells including endothelial cells and macrophages in the vessel wall.These signaling responses require the HDL receptor,scavenger receptor class B type 1（SR-B1）,an adaptor protein（PDZK1） that binds to the cytosolic C terminus of SR-B1,Akt1 activation and（at least in endothelial cells） activation of endothelial NO synthase（eNOS）.Mouse models of atherosclerosis,exemplified by apolipoprotein E or low density lipoprotein receptor gene inactivated mice（apoE or LDLR KO） develop atherosclerosis in their aortas but appear generally resistant to coronary artery atherosclerosis.On the other hand,inactivation of each of the components of HDL signaling（above）in either apoE or LDLR KO mice renders them susceptible to extensive coronary artery atherosclerosis suggesting that HDL signaling may play an important role in protection against coronary artery disease.展开更多
ntroduction: Due to widespread incidence and prevalence, human immunodeficiency virus (HIV), hepatitis B (HBV) and C (HCV), syphilis and malaria are the most common viral infections transmitted via blood transfusions....ntroduction: Due to widespread incidence and prevalence, human immunodeficiency virus (HIV), hepatitis B (HBV) and C (HCV), syphilis and malaria are the most common viral infections transmitted via blood transfusions. Yet there is insufficient information available about the exact prevalence of these infections among blood donors in Pakistan. The objectives of current study are to highlight the importance of blood screening among blood donors by finding the prevalence of high-risk transmissible diseases such as hepatitis B, hepatitis C, HIV, syphilis and malaria in the population of Nawabshah (Sindh) and to compare it with other provinces. Methods: A descriptive type of cross-sectional study was conducted on 37,845 blood donor volunteers at the blood bank of a tertiary care hospital, Peoples University of Medical and Health Sciences Hospital, in Nawabshah Sindh from 1st January 2018 to 31st December 2018. The data was taken from the well-maintained yearly record of the hospital blood bank. Analysis of all the data regarding blood donors was done using Statistical Program of Special Sciences (SPSS) version 20. Results: The most common age group was between 18 - 40 years. Donors were predominantly male (90%). HBV is the most prevalent disease amongst the blood donors of Peoples Medical College with a prevalence of 6.52% while HIV, HCV and malaria were 6.33%, 5.83% and 1.24% respectively whereas Syphilis is the least prevalent disease with a prevalence of 1.11%. Comparing with other provinces, the highest prevalence of hepatitis B was seen in Peoples Medical College, Nawabshah, Sindh whereas for hepatitis C it was the highest in Isra University Hospital, Hyderabad, Sindh. Moreover, Hayatabad Medical Complex in Peshawar was the least burdened with Hepatitis B (1.46%) and C (1.34%). Conclusion: Accidental diagnosis of blood-borne infections including Hepatitis B, C, HIV, Syphilis and malaria highlights importance of blood screening among the blood donors. There is a need of public education regarding infection prevention and transmission.展开更多
基金Supported by Program of Zhejiang Provincial TCM Sci-tech Plan,No.2024ZL039.
文摘BACKGROUND The prevalence of nonalcoholic fatty liver disease(NAFLD)in patients with chronic hepatitis B(CHB)has increased in recent clinical practice;however,the relationship between CHB and hepatic steatosis(HS)remains controversial.AIM To shed light on the potential association between NAFLD and hepatitis B virus(HBV)infection.METHODS We conducted a systematic literature search using multiple databases,including PubMed,the Cochrane Library,Web of Science,and EMBASE,to identify relevant studies.Predefined inclusion criteria were used to determine the eligibility of the studies for further analysis.RESULTS Comprehensive meta-analysis software was used for statistical analysis,which covered 20 studies.The results indicated a lower NAFLD susceptibility in HBVinfected individuals(pooled OR=0.87;95%CI=0.69-1.08;I2=91.1%),with diabetes(P=0.015),body mass index(BMI;P=0.010),and possibly age(P=0.061)as heterogeneity sources.Of note,in four studies(6197 HBV patients),HBV-infected individuals had a reduced NAFLD risk(OR=0.68,95%CI=0.51-0.89,P=0.006).A positive link between hyperlipidemia and metabolic syndrome emerged in hepatitis B patients,along with specific biochemical indicators,including BMI,creatinine,uric acid,fasting blood glucose,and homeostasis model assessment of insulin resistance.CONCLUSION HBV infection may provide protection against HS;however,the occurrence of HS in patients with HBV infection is associated with metabolic syndrome and specific biochemical parameters.
基金supported by a grant from the National Natural Science Foundation of China(491010-N11026)
文摘BACKGROUND:Non-alcoholic fatty liver disease(NAFLD)is one of the most frequent causes of liver diseases,with markedly increased prevalence.However,its mechanisms are not clear.The present study was undertaken to illustrate the role of caveolin-1(cav1)and the scavenger receptor class B type 1(SR-B1)in NAFLD.METHODS:Adult male C57BL/6 mice were fed with a normal diet or high fat and cholesterol(HFC)diet for 14 weeks.The mice were sacrificed to collect plasma and harvest the liver;their plasma lipid concentration was measured.Hepatic cav1and SR-B1 mRNA and protein expression were determined by real-time quantitative polymerase chain reaction(qPCR)and Western blotting,respectively.In order to study cav1 and SR-B1distribution and change in hepatocytes,immunohistochemical analysis was performed.RESULTS:HFC diet increased plasma lipids,induced NAFLD and increased the liver/body weight ratio.Compared to the control mice(n=6),the mRNA and protein levels of cav1 and SR-B1 in liver tissue of the NAFLD mice(n=12)increased significantly(cav1 mRNA:1.536±0.226 vs 0.980±0.272,P【0.05;protein:0.643±0.240 vs 0.100±0.130,P【0.01;SR-B1 mRNA:1.377±0.125 vs 0.956±0.151,P【0.01;protein:2.156±0.507vs 0.211±0.211,P【0.01).Furthermore,both cav1 and SR-B1immunoreactivity increased and their distribution was also changed,mainly in the plasma membrane of hepatocytes,cytoplasm and membrane of lipid droplets and around.CONCLUSION:NAFLD is associated with increased concentration of plasma lipids and upregulation of hepatic cav1 and SR-B1 gene and protein expressions,which indicate that cav1 and SR-B1 might play crucial roles in the pathogenesis of NAFLD.
基金supported by funds from the Canadian Institutes of Health Research (MOP74765)the Heart and Stroke Foundation of Canada(G-13-0002833 and G-15-0009016)
文摘Atherosclerosis is a leading underlying factor in cardiovascular disease and stroke,important causes of morbidity and mortality across the globe.Abundant epidemiological studies demonstrate that high levels of high density lipoprotein（HDL） are associated with reduced risk of atherosclerosis and preclinical,animal model studies demonstrate that this association is causative.Understanding the molecular mechanisms underlying the protective effects of HDL will allow more strategic approaches to development of HDL based therapeutics.Recent evidence suggests that an important aspect of the ability of HDL to protect against atherosclerosis is its ability to trigger signaling responses in a variety of target cells including endothelial cells and macrophages in the vessel wall.These signaling responses require the HDL receptor,scavenger receptor class B type 1（SR-B1）,an adaptor protein（PDZK1） that binds to the cytosolic C terminus of SR-B1,Akt1 activation and（at least in endothelial cells） activation of endothelial NO synthase（eNOS）.Mouse models of atherosclerosis,exemplified by apolipoprotein E or low density lipoprotein receptor gene inactivated mice（apoE or LDLR KO） develop atherosclerosis in their aortas but appear generally resistant to coronary artery atherosclerosis.On the other hand,inactivation of each of the components of HDL signaling（above）in either apoE or LDLR KO mice renders them susceptible to extensive coronary artery atherosclerosis suggesting that HDL signaling may play an important role in protection against coronary artery disease.
文摘ntroduction: Due to widespread incidence and prevalence, human immunodeficiency virus (HIV), hepatitis B (HBV) and C (HCV), syphilis and malaria are the most common viral infections transmitted via blood transfusions. Yet there is insufficient information available about the exact prevalence of these infections among blood donors in Pakistan. The objectives of current study are to highlight the importance of blood screening among blood donors by finding the prevalence of high-risk transmissible diseases such as hepatitis B, hepatitis C, HIV, syphilis and malaria in the population of Nawabshah (Sindh) and to compare it with other provinces. Methods: A descriptive type of cross-sectional study was conducted on 37,845 blood donor volunteers at the blood bank of a tertiary care hospital, Peoples University of Medical and Health Sciences Hospital, in Nawabshah Sindh from 1st January 2018 to 31st December 2018. The data was taken from the well-maintained yearly record of the hospital blood bank. Analysis of all the data regarding blood donors was done using Statistical Program of Special Sciences (SPSS) version 20. Results: The most common age group was between 18 - 40 years. Donors were predominantly male (90%). HBV is the most prevalent disease amongst the blood donors of Peoples Medical College with a prevalence of 6.52% while HIV, HCV and malaria were 6.33%, 5.83% and 1.24% respectively whereas Syphilis is the least prevalent disease with a prevalence of 1.11%. Comparing with other provinces, the highest prevalence of hepatitis B was seen in Peoples Medical College, Nawabshah, Sindh whereas for hepatitis C it was the highest in Isra University Hospital, Hyderabad, Sindh. Moreover, Hayatabad Medical Complex in Peshawar was the least burdened with Hepatitis B (1.46%) and C (1.34%). Conclusion: Accidental diagnosis of blood-borne infections including Hepatitis B, C, HIV, Syphilis and malaria highlights importance of blood screening among the blood donors. There is a need of public education regarding infection prevention and transmission.
