According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was...According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced. The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437.6×103. The precisely sequencing of 5′ and 3′ termini is undertaking.展开更多
Classical swine fever （CSF）, a list A disease of Office International des Epizooties, is caused by classical swine fever virus （CSFV） belonging to the Flaviviridae family. The well-known lapinized Chinese strain o...Classical swine fever （CSF）, a list A disease of Office International des Epizooties, is caused by classical swine fever virus （CSFV） belonging to the Flaviviridae family. The well-known lapinized Chinese strain of CSFV, also known as C-strain, was developed in China in the mid-1950s. In the past half a century, the vaccine has been proved to be safe and immunogenic in pigs of essentially any age. It is of high efficacy, providing immunized animals with broad-spectrum, sometimes lifelong, protection, which is contributed by both cell-mediated immunity and humoral immunity, against essentially all genotypes or subgenotypes of the virus. The maternal antibodies derived from immunized sows can confer solid protection of their offspring from disease; however, they have been proved to inhibit the successful active immunization of C-strain vaccine. The complete genomes of C-strain and dozens of established or field strains have been sequenced and annotated. Recently, the reverse genetics system of C-strain has been developed, resulting in several C- strain-derived candidate marker vaccines. Many countries manage to control or even eradicate CSF with the aid of mass vaccination with C-strain. in spite of these efforts, the eradication of the disease worldwide remains a big challenge and needs to go a long way, and provably still resorts to genetically modified C-strain vaccine. The authors present an overview of the characteristics of the vaccine, which has stood the test of half a century.展开更多
Effects of attenuated highly pathogenic pig reproductive and respiratory syndrome（HP-PRRS）TJM-F92 strain vaccine on immune antibody level against classical swine fever（CSF）and foot-and-mouth disease（FMD）were stu...Effects of attenuated highly pathogenic pig reproductive and respiratory syndrome（HP-PRRS）TJM-F92 strain vaccine on immune antibody level against classical swine fever（CSF）and foot-and-mouth disease（FMD）were studied from October 8 to November 12 in 2014,in order to optimize vaccination program of CSF,HP-PRRS and FMD and to provide scientific guidance for animal disease control and prevention work.The results showed that attenuated HP-PRRS（TJMF92 strain）vaccine had no significant effect on immune antibody level of hog cholera lapinized virus（HCLV,ST passage cell vaccine）attenuated vaccine and FMD-O inactivated vaccines（OZK/93 strain）,and single or combined use of three vaccines received good immunization effects.展开更多
The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the...The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% between the isolate and the classical strains (VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains (JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.展开更多
This paper presents the mathematical model consisting of conservation and balance laws (CBL) of classical continuum mechanics (CCM) and the constitutive theories derived using entropy inequality and representation the...This paper presents the mathematical model consisting of conservation and balance laws (CBL) of classical continuum mechanics (CCM) and the constitutive theories derived using entropy inequality and representation theorem for thermoviscoelastic solids (TVES) matter without memory. The CBL and the constitutive theories take into account finite deformation and finite strain deformation physics. This mathematical model is thermodynamically and mathematically consistent and is ideally suited to study nonlinear dynamics of TVES and dynamic bifurcation and is used in the work presented in this paper. The finite element formulations are constructed for obtaining the solution of the initial value problems (IVPs) described by the mathematical models. Both space-time coupled as well as space-time decoupled finite element methods are considered for obtaining solutions of the IVPs. Space-time coupled finite element formulations based on space-time residual functional (STRF) that yield space-time variationally consistent space-time integral forms are considered. This approach ensures unconditional stability of the computations during the entire evolution. In the space-time decoupled finite element method based on Galerkin method with weak form for spatial discretization, the solutions of nonlinear ODEs in time resulting from the decoupling of space and time are obtained using Newmark linear acceleration method. Newton’s linear method is used to obtain converged solution for the nonlinear system of algebraic equations at each time step in the Newmark method. The different aspects of the deformation physics leading to the factors that influence nonlinear dynamic response and dynamic bifurcation are established using the proposed mathematical model, the solution method and their validity is demonstrated through model problem studies presented in this paper. Energy methods and superposition techniques in any form including those used in obtaining solutions are neither advocated nor used in the present work as these are not supported by calculus of variations and mathematical classification of differential operators appearing in nonlinear dynamics. The primary focus of the paper is to address various aspects of the deformation physics in nonlinear dynamics and their influence on dynamic bifurcation phenomenon using mathematical models strictly based on CBL of CCM using reliable unconditionally stable space-time coupled solution methods, which ensure solution accuracy or errors in the calculated solution are always identified. Many model problem studies are presented to further substantiate the concepts presented and discussed in the paper. Investigations presented in this paper are also compared with published works when appropriate.展开更多
文摘According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced. The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437.6×103. The precisely sequencing of 5′ and 3′ termini is undertaking.
文摘Classical swine fever （CSF）, a list A disease of Office International des Epizooties, is caused by classical swine fever virus （CSFV） belonging to the Flaviviridae family. The well-known lapinized Chinese strain of CSFV, also known as C-strain, was developed in China in the mid-1950s. In the past half a century, the vaccine has been proved to be safe and immunogenic in pigs of essentially any age. It is of high efficacy, providing immunized animals with broad-spectrum, sometimes lifelong, protection, which is contributed by both cell-mediated immunity and humoral immunity, against essentially all genotypes or subgenotypes of the virus. The maternal antibodies derived from immunized sows can confer solid protection of their offspring from disease; however, they have been proved to inhibit the successful active immunization of C-strain vaccine. The complete genomes of C-strain and dozens of established or field strains have been sequenced and annotated. Recently, the reverse genetics system of C-strain has been developed, resulting in several C- strain-derived candidate marker vaccines. Many countries manage to control or even eradicate CSF with the aid of mass vaccination with C-strain. in spite of these efforts, the eradication of the disease worldwide remains a big challenge and needs to go a long way, and provably still resorts to genetically modified C-strain vaccine. The authors present an overview of the characteristics of the vaccine, which has stood the test of half a century.
文摘Effects of attenuated highly pathogenic pig reproductive and respiratory syndrome（HP-PRRS）TJM-F92 strain vaccine on immune antibody level against classical swine fever（CSF）and foot-and-mouth disease（FMD）were studied from October 8 to November 12 in 2014,in order to optimize vaccination program of CSF,HP-PRRS and FMD and to provide scientific guidance for animal disease control and prevention work.The results showed that attenuated HP-PRRS（TJMF92 strain）vaccine had no significant effect on immune antibody level of hog cholera lapinized virus（HCLV,ST passage cell vaccine）attenuated vaccine and FMD-O inactivated vaccines（OZK/93 strain）,and single or combined use of three vaccines received good immunization effects.
基金Supported by National Natural Science Foundation of China(31572550)Shandong Provincial Mod-ern Agricultural Industry and Technology System(SDAIT)+1 种基金Agricultural Science and Technology In-novation Projects of Shandong Academy of Agricultural Sciences(CXGC2016B14,CXGC2018E11,CXGC2018E10)Taishan Scholar Project
文摘The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% between the isolate and the classical strains (VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains (JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.
文摘This paper presents the mathematical model consisting of conservation and balance laws (CBL) of classical continuum mechanics (CCM) and the constitutive theories derived using entropy inequality and representation theorem for thermoviscoelastic solids (TVES) matter without memory. The CBL and the constitutive theories take into account finite deformation and finite strain deformation physics. This mathematical model is thermodynamically and mathematically consistent and is ideally suited to study nonlinear dynamics of TVES and dynamic bifurcation and is used in the work presented in this paper. The finite element formulations are constructed for obtaining the solution of the initial value problems (IVPs) described by the mathematical models. Both space-time coupled as well as space-time decoupled finite element methods are considered for obtaining solutions of the IVPs. Space-time coupled finite element formulations based on space-time residual functional (STRF) that yield space-time variationally consistent space-time integral forms are considered. This approach ensures unconditional stability of the computations during the entire evolution. In the space-time decoupled finite element method based on Galerkin method with weak form for spatial discretization, the solutions of nonlinear ODEs in time resulting from the decoupling of space and time are obtained using Newmark linear acceleration method. Newton’s linear method is used to obtain converged solution for the nonlinear system of algebraic equations at each time step in the Newmark method. The different aspects of the deformation physics leading to the factors that influence nonlinear dynamic response and dynamic bifurcation are established using the proposed mathematical model, the solution method and their validity is demonstrated through model problem studies presented in this paper. Energy methods and superposition techniques in any form including those used in obtaining solutions are neither advocated nor used in the present work as these are not supported by calculus of variations and mathematical classification of differential operators appearing in nonlinear dynamics. The primary focus of the paper is to address various aspects of the deformation physics in nonlinear dynamics and their influence on dynamic bifurcation phenomenon using mathematical models strictly based on CBL of CCM using reliable unconditionally stable space-time coupled solution methods, which ensure solution accuracy or errors in the calculated solution are always identified. Many model problem studies are presented to further substantiate the concepts presented and discussed in the paper. Investigations presented in this paper are also compared with published works when appropriate.
