Clematichinenoside protects blood brain barrier against ischemic stroke superimposed on systemic inflammatory challenges through up-regulating A20

Suppression of excessive inflammation can ameliorate blood brain barrier （BBB） injury, which shows therapeutic potential for clinical treatment of brain injury induced by stroke superimposed on systemic inflammatory diseases. In this study, we investigated whether and how clematichinenoside （AR）, an anti-inflammatory triter- pene saponin, protects brain injury from stroke superimposed on systemic inflammation. Lipopolysaccharide （LPS） was intraperitoneally injected immediately after middle cerebral artery occlusion （MCAO） in rats. Rat microvessel endothelial cells （rBMECs） were exposed to hypoxia/reoxygenation （H/R） coexisting with LPS. The results re- vealed that AR suppressed the excessive inflammation, restored BBB dysfunction, alleviated brain edema, de- lessened neurological dysfunction, and decreased infarct rate. Further study demon- creased neutrophil infiltration, inducible nitric oxide synthase （iNOS） , strated that the expression of nucleus nuclear factor kappa B （NF-KB）, intercellular adhesion molecule-1 （ ICAM-1 ）, tumor necrosis factor-α （TNF-α） and interlukin-1β （ IL-1β） were suppressed by AR via zinc finger protein A20. Besides, AR increased in vitro BBB integrity through A20. In con- clusion, AR alleviated cerebral inflammatory injury through A20-NF-KB signal pathway, offering an alternative medication for stroke associated with systemic inflammatory diseases.

东北铁线莲中皂苷类化学成分研究

目的研究东北铁线莲Clematis manshurica的化学成分。方法采用硅胶柱色谱、凝胶色谱及高压制备色谱等方法对东北铁线莲的三萜皂苷类成分进行分离纯化,并通过其理化性质及波谱数据对分离得到的化合物进行结构表征。结果从东北铁线莲中共分离得到10个齐墩果烷型三萜皂苷类化合物,分别鉴定为威灵仙皂苷L(1)、clematichinenoside A(2)、clematochinenoside F(3)、clematernoside A(4)、clematichinenoside B(5)、clematichinenoside C(6)、clematomandshurica saponin B(7)、clematomandshurica saponin D(8)、clematomandshurica saponin C(9)、huzhangoside B(10)。结论化合物1为新的三萜皂苷类化合物,2~6为首次从该植物中分离得到。