Abstract:Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted (SAD) rats. Methods Rats were divided randomly into Sham group, SAD group, SAD + hydrogen-rich saline...Abstract:Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted (SAD) rats. Methods Rats were divided randomly into Sham group, SAD group, SAD + hydrogen-rich saline (5 mL · kg^-1 · d^-1) group and SAD + hydrogen-rich saline (10 mL · kg^-1 · d^-1) group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde (MDA) in plasma and Ca2+ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2+ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.展开更多
文摘Abstract:Aim To investigate the effect of hydrogen-rich saline on arterial thrombosis in sinoaortic denervatted (SAD) rats. Methods Rats were divided randomly into Sham group, SAD group, SAD + hydrogen-rich saline (5 mL · kg^-1 · d^-1) group and SAD + hydrogen-rich saline (10 mL · kg^-1 · d^-1) group. Four weeks after Sham or SAD operation, rats were given intraperitoneally normal saline or hydrogen-rich saline for 4 weeks. Carotid artery thrombosis was induced by 30% ferric chloride. Time till occlusion was measured by Laser Doppler Flowme- try. Results When compared with the Sham group, time till occlusion was significantly shorter in SAD group. Moreover, platelet aggregation, platelet adhesion to collagen and the level of ROS and malondialdehyde (MDA) in plasma and Ca2+ in platelets were significantly raised. The level of NO and SOD in plasma and NO in platelet were significantly reduced. When compared with the SAD group, time till occlusion in hydrogen-rich saline group group was significantly prolonged. Platelet aggregation, adhesion, the level of ROS, MDA and Ca2+ were significantly re- duced, whereas the level of NO and SOD in plasma and NO in platelets were significantly higher. Conclusion Hydrogen-rich saline might improve arterial thrombosis in SAD rats through inhibition of platelet activation and oxi- dative stress.