Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent t...Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent the penetration of chemotherapeutic agents through the blood-brain barrier(BBB).To overcome this limitation,nanoparticles are taken up and transported bymacrophage and then delivered directly into the CNs.In this study,we used macrophage touptake the folate-targeted bifunctional miclles loaded with near-infrared(NIR)dye ICG-Der-01and investigate the dynamic bio-distributions of macrophage after intravenous injection intotumor-bearing mice.In vitro cellular experiments by confocal microscopy indicated that theuptake of micelles in macrophage was greatly enhanced due to the folate receptor overexpression.Dynamic bio-distributions of macrophage showed a rapid clearing rate through the liver int estine path way.In conclusion,macrophage could potentially be used as nanoparticle drug carriers andrequire further investigation.展开更多
基金grateful to Natural Science Foundation Committee of China(NSFC 81220108012,81171395,81071194,81000666,30970776,30672015,30800257and 31050110123)the Ministry of Science and Technology(2009ZX09310-004)the Priority Academic Program Development of Jiangsu Higher Education Institutions for their financial support.
文摘Treatment of malignant brain tumors continues to challenge scientists and clinicians alike.Location of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent the penetration of chemotherapeutic agents through the blood-brain barrier(BBB).To overcome this limitation,nanoparticles are taken up and transported bymacrophage and then delivered directly into the CNs.In this study,we used macrophage touptake the folate-targeted bifunctional miclles loaded with near-infrared(NIR)dye ICG-Der-01and investigate the dynamic bio-distributions of macrophage after intravenous injection intotumor-bearing mice.In vitro cellular experiments by confocal microscopy indicated that theuptake of micelles in macrophage was greatly enhanced due to the folate receptor overexpression.Dynamic bio-distributions of macrophage showed a rapid clearing rate through the liver int estine path way.In conclusion,macrophage could potentially be used as nanoparticle drug carriers andrequire further investigation.
