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红系祖细胞在造血缺陷斑马鱼体内的移植 被引量:6
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作者 李志操 黄波 +5 位作者 向文碧 孙琮杰 吴西军 周艳华 何志旭 舒莉萍 《中国比较医学杂志》 CAS 北大核心 2019年第5期84-89,共6页
目的评估红系祖细胞在先天原始造血缺陷cloche^(-/-)突变体斑马鱼体内的移植效果。方法收集绿色荧光标记红系祖细胞的转基因系zTg(gata1:EGFP)斑马鱼胚胎,制备成单细胞悬液,经流式细胞仪分选出携带绿色荧光的gata1^+细胞,利用显微注射... 目的评估红系祖细胞在先天原始造血缺陷cloche^(-/-)突变体斑马鱼体内的移植效果。方法收集绿色荧光标记红系祖细胞的转基因系zTg(gata1:EGFP)斑马鱼胚胎,制备成单细胞悬液,经流式细胞仪分选出携带绿色荧光的gata1^+细胞,利用显微注射技术将gata1^+细胞移植到42hpf的cloche^(-/-)突变体斑马鱼心脏中,采用体视荧光显微镜追踪观察移植后的红系祖细胞在cloche^(-/-)突变体斑马鱼中的表达情况。结果成功分选出携带绿色荧光的gata1^+细胞,并在移植后2 h可观察到携带绿色荧光的gata1^+细胞逐渐增殖扩散且16 h持续有绿色荧光表达。结论红系祖细胞有重建造血的潜力,为进一步研究红系祖细胞移植后的功能鉴定提供实验依据。 展开更多
关键词 红系祖细胞 斑马鱼 移植 cloche^-/-突变
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African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs 被引量:10
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作者 Dan Li Yinguang Liu +6 位作者 Xiaolan Qi Yuan Wen Pan Li Zhao Ma Yongjie Liu Haixue Zheng Zhijie Liu 《Virologica Sinica》 SCIE CAS CSCD 2021年第2期187-195,共9页
African swine fever virus(ASFV)is the etiological agent of African swine fever(ASF),an often lethal disease in domestic and wild pigs.ASF represents a major threat to the swine industry worldwide.Currently,no commerci... African swine fever virus(ASFV)is the etiological agent of African swine fever(ASF),an often lethal disease in domestic and wild pigs.ASF represents a major threat to the swine industry worldwide.Currently,no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns.Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge.In the present study,a mutant ASFV strain with the deletion of ASFV MGF-110-9L(ASFV-D9L)was generated from a highly virulent ASFV CN/GS/2018 parental strain,a genotypeⅡASFV.Relative to the parental ASFV isolate,deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-D9L to replicate in vitro in primary swine macrophage cell cultures.The majority of animals inoculated intramuscularly with a low dose of ASFV-D9L(10 HAD50)remained clinically normal during the 21-day observational period.Three of five ASFV-D9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response,indicating partial attenuation of the ASFV-D9L strain in pigs.The findings imply the potential usefulness of the ASFV-D9L strain for further development of ASF control measures. 展开更多
关键词 African swine fever virus(ASFV) MGF-110-9L mutant Attenuated virulence PIG
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Crystallization of Nitrogenase MoFe Protein (NifB Av1) from a nifB Mutated Strain UW45 of Azotobacter vinelandii
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作者 赵颖 吕玉兵 +4 位作者 赵剑峰 周军贤 潜忠兴 王耀萍 黄巨富 《Acta Botanica Sinica》 CSCD 2003年第7期820-824,共5页
Six hundred and 28 mg of NifB(-) Av1 was obtained by a chromatography twice on DE 52 columns and Sephacryl S-300 column from the crude extract (37 677 mg) of a nifB mutated strain UW45 of Azotobacter vinelandii Lipman... Six hundred and 28 mg of NifB(-) Av1 was obtained by a chromatography twice on DE 52 columns and Sephacryl S-300 column from the crude extract (37 677 mg) of a nifB mutated strain UW45 of Azotobacter vinelandii Lipmann. The protein was almost homogeneous as determined by Coomassie staining of SDS gels. The analysis by SDS-PAGE showed that NifB(-)Av1 was similar to Av1 from wild-type strain of A. vinelandii (OP) in the kinds of subunits (alpha and beta subunit). When complemented with Av2, NifB(-)Av1 had hardly any H-reducing activity, but could be significantly activated by FeMoco extracted from Av1. Under a suitable condition for crystallization, short dark-brown rhombohedral crystals could be obtained from NifB(-)Av1. Both of the longest sides of the biggest crystal were 0.1 mm. The time of the formation of crystals and number, size, quality and shape of crystals obviously depended not only on the kinds and concentrations of the components in the precipitant solution, but also on the methods for crystallization and technical bias, etc. The preliminary results showed that the crystal seemed to be formed from NifB(-)Av1. 展开更多
关键词 mutant strain UW45 of Azotobacter vinelandii nitrogenase NifB(-)Av1 CRYSTALLIZATION
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Cancer-derived C-terminus-extended p53 mutation confers dominant-negative effect on its wild-type counterpart
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作者 Shibo Huang Bo Cao +6 位作者 Jieqiong Wang Yiwei Zhang Elisa Ledet Oliver Sartor Yuqin Xiong Shelya X.Zeng Hua Lu 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2022年第1期11-20,共10页
The vast majority of p53 missense mutants lose the wild-type (wt) function and/or exert ‘dominant-negative’ effects on their wt counterpart. Here, we identify a novel form of p53 mutation with an extended C-terminus... The vast majority of p53 missense mutants lose the wild-type (wt) function and/or exert ‘dominant-negative’ effects on their wt counterpart. Here, we identify a novel form of p53 mutation with an extended C-terminus (p53 long C-terminus, p53LC) in a variety of human cancers. Interestingly, the two representative mutants (named ‘p53-374*48’ and ‘p53-393*78’) as tested in this study show both loss-of-function and dominant-negative phenotypes in cell proliferation and colony formation assays. Mechanistically, p53LCs interact with and retain wt p53 in the cytoplasm and prevent it from binding to the promoters of target genes, consequently inhibiting its transcriptional activity. Also, p53LCs are very stable, though not acetylated in cells. Remarkably, the p53LCs can desensitize wt p53-containing cancer cells to p53-activating agents. Together, our results unveil a longer form of p53 mutant that possesses a dominant-negative effect on its wt counterpart, besides losing its wt activity. 展开更多
关键词 mutant p53-393*78 mutant p53-374*48 P53 longer C-terminus p53 dominant-negative effect drug resistance ACETYLATION
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Chromatin architectural alterations due to null mutation of a major CG methylase in rice
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作者 Jinbin Wang Xiaochong Li +13 位作者 Qianli Dong Changping Li Juzuo Li Ning Li Baoxu Ding Xiaofei Wang Yanan Yu Tianya Wang Zhibin Zhang Yiyang Yu Man Lang Zixian Zeng Bao Liu Lei Gong 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2022年第12期2396-2410,共15页
Associations between 3D chromatin architectures and epigenetic modifications have been characterized in animals.However,any impact of DNA methylation on chromatin architecture in plants is understudied,which is confin... Associations between 3D chromatin architectures and epigenetic modifications have been characterized in animals.However,any impact of DNA methylation on chromatin architecture in plants is understudied,which is confined to Arabidopsis thaliana.Because plant species differ in genome size,composition,and overall chromatin packing,it is unclear to what extent findings from A.thaliana hold in other species.Moreover,the incomplete chromatin architectural profiles and the low-resolution high-throughput chromosome conformation capture(Hi-C)data from A.thaliana have hampered characterizing its subtle chromatin structures and their associations with DNA methylation.We constructed a high-resolution Hi-C interaction map for the null OsMET1-2(the major CG methyltransferase in rice)mutant(osmet1-2)and isogenic wild-type rice(WT).Chromatin structural changes occurred in osmetl-2,including intra-/inter-chromosomal interactions,compartment transition,and topologically associated domains(TAD)variations.Our findings provide novel insights into the potential function of DNA methylation in TAD formation in rice and confirmed DNA methylation plays similar essential roles in chromatin packing in A,thaliana and rice. 展开更多
关键词 3D chromatin architectures DNA methylation Hi-C osmet1-2 mutant
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