Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and repair.We previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological recovery.However,the mechanism of CXCR4 upregulation in induced neural stem cells remains unclear.In this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 expression.However,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial activation.In agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury mice.Notably,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head injury.In conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neural stem cells on microglial activation via upregulation of CXCR4 and Crry,thereby promoting induced neural stem cell–mediated improvement of neuronal injury,cerebral edema,and neurological disorders following closed head injury.

Sensing-Communication-Computing-Control Closed Loop for NOMA-UAV Systems

In the areas without terrestrial communication infrastructures,unmanned aerial vehicles(UAVs)can be utilized to serve field robots for mission-critical tasks.For this purpose,UAVs can be equipped with sensing,communication,and computing modules to support various requirements of robots.In the task process,different modules assist the robots to perform tasks in a closed-loop way,which is referred to as a sensing-communication-computing-control(SC3)loop.In this work,we investigate a UAV-aided system containing multiple SC^(3)loops,which leverages non-orthogonal multiple access(NOMA)for efficient resource sharing.We describe and compare three different modelling levels for the SC^(3)loop.Based on the entropy SC^(3)loop model,a sum linear quadratic regulator(LQR)control cost minimization problem is formulated by optimizing the communication power.Further for the assure-to-be-stable case,we show that the original problem can be approximated by a modified user fairness problem,and accordingly gain more insights into the optimal solutions.Simulation results demonstrate the performance gain of using NOMA in such task-oriented systems,as well as the superiority of our proposed closed-loop-oriented design.

Advancing neuroscience through real-time processing of big data:Transition from open-loop to closed-loop paradigms

The brain functions as a closed-loop system that continuously generates behavior in response to the external environment and adjusts actions based on the outcomes.Traditional research methodologies in neuroscience,especially those employed in brain imaging experiments,have mainly adopted an open-loop paradigm(Grosenick et al.,2015).Functional neural circuits are analyzed offline and subsequently tested through manipulation of neuronal activities within specific regions or with genetic markers.By establishing a closed-loop research paradigm,functional ensembles can be detected and tested in real time with temporal sequences.These functional ensembles,rather than brain regions or genetically labeled neural populations,serve as fundamental units of neural networks,offering valuable insights into the dissection of neural circuits.The closed-loop research paradigm also enables the capture of high-dimensional activities of internal brain dynamics and precise elucidation of physiological processes such as learning,decision-making,and sleep.

Design and implementation of digital closed-loop drive control system of a MEMS gyroscope

In order to effectively control the working state of the gyroscope in drive mode, the drive characteristics of the micro electromechanical system （MEMS） gyroscope are analyzed in principle. A novel drive circuit for the MEMS gyroscope in digital closed-loop control is proposed, which utilizes a digital phase-locked loop （PLL） in frequency control and an automatic gain control （AGC） method in amplitude control. A digital processing circuit with a field programmable gate array （FPGA） is designed and the experiments are carried out. The results indicate that when the temperature changes, the drive frequency can automatically track the resonant frequency of gyroscope in drive mode and that of the oscillating amplitude holds at a set value. And at room temperature, the relative deviation of the drive frequency is 0.624 ×10^-6 and the oscillating amplitude is 8.0 ×10^-6, which are 0. 094% and 18. 39% of the analog control program, respectively. Therefore, the control solution of the digital PLL in frequency and the AGC in amplitude is feasible.

Closed-Loop MIMO系统线性预编码技术

本文讨论了Closed-Loop MIMO系统在有限反馈资源下如何进行反馈的方法,即两种发射端线性预编码的思路,特别是其中的反馈码本设计问题。由于需要接收端向发射端反馈信道信息,而上行反馈资源又非常有限,所以发射端线性预编码技术成为解决把MIMO技术应用于新一代蜂窝系统或无线局域网的关键问题之一,具有无限广阔的发展前景。

Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

Phase-dependent inversionless gain in a four-level atomic system with a closed interaction loop

A four-level atomic system with a closed interaction loop connected by two coherent driving fields and a microwave field is investigated. The results show that inversionless gain can be achieved on a higher frequency transition outside the closed interaction loop, and the gain behaviour can be modulated by the phase of the closed loop as well as the amplitude of the microwave field. The phase sensitivity property in such a scheme is similar to that in an analogous configuration with spontaneously generated coherence, but it is beyond the rigorous condition of near-degenerate levels with non-orthogonal dipole moments. Therefore this scheme is much more convenient in experimental realization.

Accuracy Compensation Technology of Closed⁃Loop Feedback of Industrial Robot Joints

The existing kinematic parameter calibration method cannot further improve the absolute positioning accuracy of the robot due to the uncertainty of positioning error caused by robot joint backlash.In view of this problem,a closed‑loop feedback accuracy compensation method for robot joints was proposed.Firstly,a Chebyshev polynomial error estimation model was established which took geometric error and non‑geometric error into account.In addition,the absolute linear grating scale was installed at each joint of the robot and the positioning error of the robot end was mapped to the joint angle.And the joint angle corrected value was obtained.Furthermore,the closed‑loop feedback of robot joints was established to realize the online correction of the positioning error.Finally,an experiment on the KUKA KR210 industrial robot was conducted to demonstrate the effectiveness of the method.The result shows that the maximum absolute positioning error of the robot is reduced by 75%from 0.76 mm to 0.19 mm.This method can compensate the robot joint backlash effectively and further improve the absolute positioning accuracy of the robot.

Method of closed loop springback compensation for incremental sheet forming process

The closed loop control model was built up for compensating the springback and enhancing the work piece precision.A coupled closed loop algorithm and a finite element method were developed to simulate and correct the springback of incremental sheet forming.A three-dimensional finite element model was established for simulation of springback in incremental sheet forming process.The closed loop algorithm of trajectory profile for the incremental sheet forming based on the wavelet transform combined with fast Fourier transform was constructed.The profile of processing tool path of shallow dishing with spherical surface was designed on the basis of the profile correction algorithm.The result shows that the algorithm can predict an ideal profile of processing track,and the springback error of incremental sheet forming is eliminated effectively.It has good convergence efficiency,and can improve the workpiece dimensional accuracy greatly.

Visual Study on Flow and Operational Characteristics of Flat Plate Closed Loop Pulsating Heat Pipes

This paper presents an experimental study including visualization on a flat plate closed loop pulsating heat pipes.It consists of a total of 40 channels with square cross section(2 mm×2 mm,165 mm long) machined directly on an aluminum plate(180 mm×120 mm×3 mm) covered by a transparent plate.The working fluid employed is ethanol.As a result,various flow patterns and their transitions are observed and found to be related to the fluid fill ratio,input heat load and the device orientation.Also the operational characteristics and working mechanism are discussed.

Electrical crosstalk-coupling measurement and analysis for digital closed loop fibre optic gyro

The phase modulation and the closed-loop controller can generate electrical crosstalk-coupling in digital closed- loop fibre optic gyro. Four electrical cross-coupling paths are verified by the open-loop testing approach. It is found the variation of ramp amplitude will lead to the alternation of gyro bias. The amplitude and the phase parameters of the electrical crosstalk signal are measured by lock-in amplifier, and the variation of gyro bias is confirmed to be caused by the alternation of phase according to the amplitude of the ramp. A digital closed-loop fibre optic gyro electrical crosstalk-coupling model is built by approximating the electrical cross-coupling paths as a proportion and integration segment. The results of simulation and experiment show that the modulation signal electrical crosstalk-coupling can cause the dead zone of the gyro when a small angular velocity is inputted, and it could also lead to a periodic vibration of the bias error of the gyro when a large angular velocity is inputted.

Parametric Influence on Thermal Performance of Flat Plate Closed Loop Pulsating Heat Pipes

This paper presents an experimental study on a flat plate closed loop pulsating heat pipes. It consisted of total 40 channels with square cross section （2 × 2 mm^2, 165 mm long） machined directly on an aluminum plate（180×120×3 nm^2）, which was covered by a transparent plate. The working fluid employed was ethanol. As the results, the influence parameters of thermal performance were investigated, such as filling ratio, heat load and operational orientations etc. Filling ratio was found to be a critical parameter, and its effect was rather complicated. According to its values the PHP plate could have four distinct working zones with different operational characteristics and heat transfer performance. The effect of heat load on thermal performance was found to be positive, and in general, iucrcasing the heat load would improve heat transfer performance. In order to analyze the effect of gravity on thermal performance, three different heat modes and total seven tilt angles were tested and compared. Successful operation at all orientations with respect to gravity was also achieved.

Dynamics and adaptive control of a dual-arm space robot with closed-loop constraints and uncertain inertial parameters

A dynamics-based adaptive control approach is proposed for a planar dual-arm space robot in the presence of closed-loop constraints and uncertain inertial parameters of the payload. The controller is capable of controlling the po- sition and attitude of both the satellite base and the payload grasped by the manipulator end effectors. The equations of motion in reduced-order form for the constrained system are derived by incorporating the constraint equations in terms of accelerations into Kane＇s equations of the unconstrained system. Model analysis shows that the resulting equations perfectly meet the requirement of adaptive controller design. Consequently, by using an indirect approach, an adaptive control scheme is proposed to accomplish position/attitude trajectory tracking control with the uncertain parameters be- ing estimated on-line. The actuator redundancy due to the closed-loop constraints is utilized to minimize a weighted norm of the joint torques. Global asymptotic stability is proven by using Lyapunov＇s method, and simulation results are also presented to demonstrate the effectiveness of the proposed approach.

An Integrated Multi-Echelon Model for a Sustainable Closed Loop Supply Chain Network Design

The integration of entire supply and value chain into a closed loop network is gaining more importance in recent times in order to ensure a business to be economically and environmentally sustainable with the changing trends in business and social environments, growing environmental consciousness in the society and government legislations to protect the environment as well as the business. In this context, this paper considers a multi-echelon closed loop supply chain network design with forward and reverse logistics components. An attempt has been made to develop a mixed integer non-linear programming model for this problem with different costs so that the sum of the total cost is minimized subject to different constraints pertaining to capacities of the entities of the system, demands of first customers and second customers. A generalized model is presented and then its application is illustrated using an example problem by solving the model using LINGO14. This model forms as a tool to compare future meta-heuristics to check the closeness of their solutions with corresponding optimal solutions.

Development and Comparison of Hybrid Genetic Algorithms for Network Design Problem in Closed Loop Supply Chain

This paper presents four different hybrid genetic algorithms for network design problem in closed loop supply chain. They are compared using a complete factorial experiment with two factors, viz. problem size and algorithm. Based on the significance of the factor “algorithm”, the best algorithm is identified using Duncan’s multiple range test. Then it is compared with a mathematical model in terms of total cost. It is found that the best hybrid genetic algorithm identified gives results on par with the mathematical model in statistical terms. So, the best algorithm out of four algorithm proposed in this paper is proved to be superior to all other algorithms for all sizes of problems and its performance is equal to that of the mathematical model for small size and medium size problems.

Experiment investigation on visualization and operating characteristics of closed loop plate oscillating heat pipe with parallel channels

Using ethanol or acetone as the working fluid, visualization of oscillations in steady state was observed visually by high-speed cameras, and temperature oscillating and heat transfer characteristics of closed-loop plate oscillating heat pipe with parallel channels(POHP-PC) were experimentally investigated by varying liquid filled ratios(50%, 70%, 85%), section scales(1 mm×1 mm and 1 mm×1.5 mm), inclination angles, working fluids and heating inputs. It was found that during operating there was mixed flow consisting of plug flow and annular flow in channels of oscillating heat pipe at steady-state. There was an equilibrium position for working fluid of condenser during oscillating, and periodic oscillations occurred up and down in the vicinity of equilibrium position. With heat input increasing, equilibrium position rose slowly as a result of vapor pressure of evaporation.Evaporation temperature oscillating amplitude possessed a trend of small-large-small and frequency trend was of small-large during steady-state. It may be generally concluded that temperature, whether evaporator or condenser, fluctuated sharply or rose continuously when oscillating heat pipe coming to dry burning state. Simultaneously, it was found that temperature difference of cooling water possibly dropped with heat input rising during dry burning state. Thermal resistance of No. 2 with acetone was lower than that of No. 1 during experiments, but No. 2 achieving heat transfer limit was earlier than No. 1. However, with ethanol, thermal resistance of No. 1 and No. 2 were similar with the heating input less than 110-120 W and filling ratios of 50% and 70%. And with filling ratio of 85%, heating transfer performance of No. 2 was better compared to No. 1 during all the experiments.

Research on perturbation of neutron fluence rate in a closed thermal neutron field due to medium materials

The neutron radiation field has vital applications in areas such as biomedicine,geology,radiation safety,and many others for neutron detection and neutron metrology.Correcting neutron fluence rate perturbation accurately is an important yet challenging problem.This study proposes a correction method that analyzes three physical processes.This method,which transforms the detection process from point detection to area detection,is based on a novel physical model and has been validated through theoretical analyses,experiments,and simulations.According to the average differences between the calculated and experimental results,the new method(1.67%)demonstrated better accuracy than the traditional simulation(2.17%).In a closed thermal neutron radiation field,the detector or strong neutron absorption material significantly perturbs the neutron fluence rate,whereas its impact on the energy spectrum shape and neutron directionality is relatively minor.Furthermore,based on the calculation results of the perturbation rate formula for medium materials with different compositions and sizes,the larger the volume and capture cross section of the medium,the higher the perturbation rate generated in the closed radiation field.

Closed Loop Hydraulic System and Its Effect on Actuator Design

Hydraulic systems provide a clean and stable supply of hydraulic fluid for subsea valves and actuators installed on the subsea bed in subsea production systems.Subsea control systems are used for contemporary subsea fields instead of installing the control system on topside.Although all-electric subsea systems are state-of-the-art with benefits such as health,safety,and environment improvement,as well as efficiency and lower cost,hydraulic systems are still used for the development of many subsea fields.One of the main questions in the selection of a subsea hydraulic field is whether to choose an open or closed loop hydraulic system.The main characteristic of an open loop hydraulic system is that the hydraulic fluid is discharged into the marine environment during the actuation of the subsea valves.Conversely,the hydraulic fluid is returned to the topside facilities through an umbilical system in a closed loop system.Given that closed loop systems are more eco-friendly,the main question in this research is to examine the effect of the actuator connection of the closed loop system on actuator design.Two cases of actuated valves connected to a closed loop system are analyzed in this paper.The first is a 71/16-in.subsea slab gate valve in the pressure class of 517 bar with a linear spring return fail-safe close(FSC)actuator located on a manifold branch.The data indicates that the piston rod and cylinder diameter of the FSC linear actuator should be increased by some millimeters due to the accumulation of hydraulic oil at the bottom of the actuator.The hydraulic oil in the closed loop system helps in closing the actuator and spring force,so the spring constant and torque should be reduced as a result.The second case involves a 16-in.subsea ball valve in the pressure class of 517 bar with a double-acting fail-as-is rack and pinion actuator.The conclusion in this case is to avoid making any change in the design of double-acting actuator in connection to the closed loop system.

On Fuzzy Regularly Closed Filters in Michálek Fuzzy Topological Space

C.L. Chang’s introduction of fuzzy topology in 1981 opened up new avenues for parallel theories in topology. However, Chang’s work appears to focus more on the topology of fuzzy sets rather than fuzzy topology itself. In 1975, Michálek presented a functional definition of ordinary topology and later developed fuzzy topology as a distinct extension of this idea, setting it apart from Chang’s approach. While there has been significant research on Chang’s fuzzy topology, Michálek’s version has not received as much attention. This paper introduces the concept of fuzzy regularly closed filters, or FRCM filters, within Michálek’s fuzzy topological space and explores some properties of FRCM ultrafilters.