Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

Application of Opening and Closing Morphology in Deep Learning-Based Brain Image Registration

In order to improve the registration accuracy of brain magnetic resonance images(MRI),some deep learning registration methods use segmentation images for training model.How-ever,the segmentation values are constant for each label,which leads to the gradient variation con-centrating on the boundary.Thus,the dense deformation field(DDF)is gathered on the boundary and there even appears folding phenomenon.In order to fully leverage the label information,the morphological opening and closing information maps are introduced to enlarge the non-zero gradi-ent regions and improve the accuracy of DDF estimation.The opening information maps supervise the registration model to focus on smaller,narrow brain regions.The closing information maps supervise the registration model to pay more attention to the complex boundary region.Then,opening and closing morphology networks(OC_Net)are designed to automatically generate open-ing and closing information maps to realize the end-to-end training process.Finally,a new registra-tion architecture,VM_(seg+oc),is proposed by combining OC_Net and VoxelMorph.Experimental results show that the registration accuracy of VM_(seg+oc) is significantly improved on LPBA40 and OASIS1 datasets.Especially,VM_(seg+oc) can well improve registration accuracy in smaller brain regions and narrow regions.

Is the Cape Stone Forest in Shantou City formed by wave erosion? A comparison of the pedestal rocks on the coasts and the mountains of Queshi in eastern Guangdong, China

Cape Stone Forest is a group of granite rock pillars(pedestal rocks) towering over Shilin Lake, on the southern shore of Shantou Bay in eastern Guangdong, China. The rock pillars were previously identified as sea stacks because they have marine notch-like concave sidewalls at their base, and more importantly, the lake is immediately adjacent to the bay, which is exposed to the open sea. However, rock pillars similar in shape and size can also be found at the top of Queshi Mountain, which is only about 300 meters northwest of the lake and about 85 meters above sea level. Therefore, the marine origin of Cape Stone Forest is seriously questioned. In this study, 3D imagery and drone technology were used to collect data in the investigations without direct manual measurements in the water or on the mountain. It shows that the concave sidewalls of the rock pillars in the lake and on the mountains occur at different heights and are exposed to different directions, while a natural sea stack on Mayu Island at the mouth of Shantou Bay has a horizontal notch parallel to the sea level, although the granite rock of the sea stack is the same as that of the lake and the mountains. The eastern side of the island, where the sea stack is located, is exposed to the open sea but blocks large waves for the rock pillars in the lake. Therefore, the origin of Cape Stone Forest cannot be explained by wave-based mechanisms. The only satisfactory explanation that takes into account all the field evidence is that the narrow rock pillars of the lake and mountain were formed by chemical weathering that penetrated closely the spaced joints of the granite rock, and the notch-like concave sidewalls were formed by more effective chemical weathering at the base of the pillars.

Free radicals trigger the closure of open pores in lignin-derived hard carbons toward improved sodium-storage capacity

The chemical activation of various precursors is effective for creating additional closed pores in hard carbons for sodium storage.However,the formation mechanism of closed pores under the influence of pore-forming agents is not well understood.Herein,an effective chemical activation followed by a high-temperature self-healing strategy is employed to generate interconnected closed pores in lignin-derived hard carbon(HCs).By systematic experimental design combined with electron paramagnetic res-onance spectroscopy,it can be found that the content of free radicals in the carbon matrix influences the closure of open pores at high temperatures.Excessively high activation temperature(>700 C)leads to a low free radical concentration,making it difficult to achieve self-healing of open pores at high tempera-tures.By activation at 700°C,a balance between pore making and self-healing is achieved in the final hard carbon.A large number of free radicals triggers rapid growth and aggregation of carbon microcrys-tals,blocking pre-formed open micropores and creating additional interconnected closed pores in as-obtained hard carbons.As a result,the optimized carbon anode(LK-700-1300)delivers a high reversible capacity of 330.8 mA h g^(-1) at 0.03 A g^(-1),which is an increase of 86 mA h g^(-1) compared to the pristine lignin-derived carbon anode(L-700-1300),and exhibits a good rate performance(202.1 mA h g^(-1) at 1 A g^(-1)).This work provides a universal and effective guidance for tuning closed pores of hard carbons from otherprecursors.

Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and repair.We previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological recovery.However,the mechanism of CXCR4 upregulation in induced neural stem cells remains unclear.In this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 expression.However,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial activation.In agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury mice.Notably,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head injury.In conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neural stem cells on microglial activation via upregulation of CXCR4 and Crry,thereby promoting induced neural stem cell–mediated improvement of neuronal injury,cerebral edema,and neurological disorders following closed head injury.

SOME PROPERTIES OF THE INTEGRATION OPERATORS ON THE SPACES F(p,q,s)

We study the closed range property and the strict singularity of integration operators acting on the spaces F(p,pα-2,s).We completely characterize the closed range property of the Volterra companion operator I_(g)on F(p,pα-2,s),which generalizes the existing results and answers a question raised in[A.Anderson,Integral Equations Operator Theory,69(2011),no.1,87-99].For the Volterra operator J_(g),we show that,for 0<α≤1,J_(g)never has a closed range on F(p,pα-2,s).We then prove that the notions of compactness,weak compactness and strict singularity coincide in the case of J_(g)acting on F(p,p-2,s).

Formation of a Rapidly Rotating Classical Be-star in a Massive Close Binary System

This paper investigates the spin-up of a mass-accreting star in a close binary system passing through the first stage of mass exchange in the Hertzsprung gap. Inside an accreting star, angular momentum is carried by meridional circulation and shear turbulence. The circulation carries part of the angular momentum of the accreted layers to the accretor's surface. The greater the rate of arrival of angular momentum in the accretor is, the greater this part. It is assumed that this part of the angular momentum can be removed by the disk further from the accretor. If the angular momentum in the matter entering the accretor is more than half the Keplerian value, then the angular momentum obtained by the accretor during mass exchange stage does not depend on the rate of arrival of angular momentum. The accretor may have the characteristics of a Be-star immediately after the end of mass exchange.

Periodic Variation Studies of the Two Short Period W UMa-type Eclipsing Binaries: LX Lyn and V0853 Aur

In this paper,new light curves(LCs) of contact eclipsing binary(CEB) systems LX Lyn and V0853 Aur are presented and analyzed by using the 2015 version of the Wilson-Devinney(W-D) code.In order to explain their asymmetric LCs,cool starspots on the components were employed.It is suggested that their fill-out degrees are f=12.0%(LX Lyn) and f=26.3%(V0853 Aur).At the same time,we found that LX Lyn is a W-type eclipsing binary(EB) with an orbital inclination of i=84°.88 and a mass ratio of q=2.31.V0853 Aur is also a W-type CEB with a mass ratio of q=2.77 and an orbital inclination of i= 79°.26.Based on all available times of light minimum,their orbital period changes are studied by using the O-C method.The O-C diagram of LX Lyn reveals a cyclic oscillation with a period of about 14.84 yr and an amplitude of 0.0019 days,which can be explained by the light-travel time effect(LTTE) due to the presence of a third body with a minimum mass of0.06M_⊙.For V0853 Aur,it is discovered that the O-C diagram of the system also shows a cyclic oscillation with a period of 9.64 yr and an amplitude of 0.03365 days.The cyclic oscillation of V0853 Aur can be attributed to the LTTE by means of a third body with a mass no less than 3.77M_⊙.The third body may play an important role in the formation and evolution of these systems.

Value of negative pressure sealing drainage on wound healing of Gustilo IIIB and IIIC open fractures

BACKGROUND Gustilo III fractures have a high incidence and are difficult to treat.Patients often experience difficulty in wound healing.Negative pressure drainage technology can help shorten wound healing time and has positive value in improving patient prognosis.AIM To explore the clinical value of the negative pressure sealing drainage technique in wound healing of Gustilo IIIB and IIIC open fractures.METHODS Eighty patients with Gustilo IIIB and IIIC open fractures with skin and soft tissue injuries who were treated in the Second People’s Hospital of Dalian from March 2019 to December 2021 were selected as the research subjects.They were divided into a study group(n=40,healed with negative pressure closed drainage)and a control group(n=40,healed with conventional dressing changes)according to the variation in the healing they received.The efficacy of the clinical interventions,the variations in the regression indicators(time to wound healing,time to fracture healing,time to hospitalization),and the conversion and healing of bacterial wounds were compared 1-3 mo after the intervention.RESULTS The total effective rate of patients among the study group was 95.00%(38/40),which was notably higher than 75.00%(30/40)among the control group(P<0.05).The wound healing time,fracture healing time,and hospital stay of the patients in the study group was shorter than the control group(P<0.05).After the intervention,the negative bacterial culture at the wound site rate and wound healing rate of the patients among the study group increased compared to the control group(P<0.05).CONCLUSION Negative pressure sealing and drainage technology has a good therapeutic effect on patients with Gustilo IIIB and IIIC open fracture wounds with skin and soft tissue injury.It can notably enhance the wound healing rate and the negative rate of bacteria on the wound surface and help to speed up the recovery process of patients.

Point-symmetry in SNR G1.9+0.3:A Supernova that Destroyed its Planetary Nebula Progenitor

I analyze a new X-ray image of the youngest supernova remnant(SNR)in the Galaxy,which is the type Ia SNR G1.9+0.3,and reveal a very clear point-symmetrical structure.Since explosion models of type Ia supernovae(SNe Ia)do not form such morphologies,the point-symmetrical morphology must come from the circumstellar material(CSM)into which the ejecta expands.The large-scale point-symmetry that I identify and the known substantial deceleration of the ejecta of SNR G1.9+0.3 suggest a relatively massive CSM of■1M⊙.I argue that the most likely explanation is the explosion of this SN Ia into a planetary nebula.The scenario that predicts a large fraction of SN Ia inside PNe(SNIPs)is the core degenerate scenario.Other SN Ia scenarios might lead to only a very small fraction of SNIPs or none at all.

V0405 Dra:A New Deep and Low Mass Ratio Contact Binary with Extremely Fast Decrease in the Orbital Period

V0405 Dra is a W UMa-type binary star.Based on the TESS data,we have conducted an orbital period study and performed a light curve analysis for the system.The orbital period study reveals that the O-C curve for V0405 Dra exhibits secular decrease at an extremely high rate of d P/dt=-2.71×10^(-6)day year^(-1),along with periodic variations characterized by an amplitude of A_(3)=0.0032 day and a period of P_(3)=1.413 years.The orbital periodic change is possibly due to the light-travel time effect resulting from an additional third body in the system,for which we estimate a minimum mass of M_(3)=0.77M_(⊙).By employing the 2013 version of the Wilson-Devinney(W-D)method to synthesize a light curve,we derived photometric solutions indicating that V0405 Dra is a new deep(f=68.7%)and low-mass ratio(q=0.175)contact binary.The fast decrease in its orbital period is likely caused by mass transfer from the more massive primary star to the less massive secondary star,or due to angular momentum loss.With further mass transfer and loss of angular momentum,the binary will gradually evolve into a tighter contact configuration,eventually leading to a merger into a single star,following the evolutionary paths suggested for such deep and low mass ratio contact binaries.

Artificial intelligence powered glucose monitoring and controlling system:Pumping module

BACKGROUND Diabetes,a globally escalating health concern,necessitates innovative solutions for efficient detection and management.Blood glucose control is an essential aspect of managing diabetes and finding the most effective ways to control it.The latest findings suggest that a basal insulin administration rate and a single,highconcentration injection before a meal may not be sufficient to maintain healthy blood glucose levels.While the basal insulin rate treatment can stabilize blood glucose levels over the long term,it may not be enough to bring the levels below the post-meal limit after 60 min.The short-term impacts of meals can be greatly reduced by high-concentration injections,which can help stabilize blood glucose levels.Unfortunately,they cannot provide long-term stability to satisfy the postmeal or pre-meal restrictions.However,proportional-integral-derivative(PID)control with basal dose maintains the blood glucose levels within the range for a longer period.AIM To develop a closed-loop electronic system to pump required insulin into the patient's body automatically in synchronization with glucose sensor readings.METHODS The proposed system integrates a glucose sensor,decision unit,and pumping module to specifically address the pumping of insulin and enhance system effectiveness.Serving as the intelligence hub,the decision unit analyzes data from the glucose sensor to determine the optimal insulin dosage,guided by a pre-existing glucose and insulin level table.The artificial intelligence detection block processes this information,providing decision instructions to the pumping module.Equipped with communication antennas,the glucose sensor and micropump operate in a feedback loop,creating a closed-loop system that eliminates the need for manual intervention.RESULTS The incorporation of a PID controller to assess and regulate blood glucose and insulin levels in individuals with diabetes introduces a sophisticated and dynamic element to diabetes management.The simulation not only allows visualization of how the body responds to different inputs but also offers a valuable tool for predicting and testing the effects of various interventions over time.The PID controller's role in adjusting insulin dosage based on the discrepancy between desired setpoints and actual measurements showcases a proactive strategy for maintaining blood glucose levels within a healthy range.This dynamic feedback loop not only delays the onset of steady-state conditions but also effectively counteracts post-meal spikes in blood glucose.CONCLUSION The WiFi-controlled voltage controller and the PID controller simulation collectively underscore the ongoing efforts to enhance efficiency,safety,and personalized care within the realm of diabetes management.These technological advancements not only contribute to the optimization of insulin delivery systems but also have the potential to reshape our understanding of glucose and insulin dynamics,fostering a new era of precision medicine in the treatment of diabetes.

Embedding an FIR in a Body

We have shown that certain non-commutative integral rings can be immersed in a field by a method which is not that of the construction of the field of fractions on the right ℝ ℝ ∗−1 , or left ℝ ∗−1 ℝ , of the ring ℝ under consideration.

Discussion on Dust Removal(Suppression)System of Crushing Station in Open-Pit Coal Mine

This paper introduces the method of coal dust treatment in crushing station and the present situation of dust removal system in typical open-pit coal mine crushing station in China,and expounds the research idea of determining comprehensive dust removal(suppression)system in crushing station inspired by the working principle of"range hood".Based on the design example and link optimization of the crush-ing station of open-pit coal mine I of Thar coalfield,this paper finally draws some conclusions on the key technologies of dust removal(suppression)system of open-pit coal mine crushing station.This study has certain reference value for the technical innovation of dust removal(suppression)system in crushing station,the realization of green mining in"crushing link",and the reduction and avoidance of ecological environment pollution in mining area.

Evaluation of the Impact of Driver Behavior on Back of Queues Events in Work Zones Using the SHRP2 Naturalistic Driving Study Data

The SHRP2 Naturalistic Driving Study was used to evaluate the impact of various work zone and driver characteristics on back of queue safety critical events (crash, near-crash, or conflicts) The model included 43 SCE and 209 “normal” events which were used as controls. The traces included representing 209 unique drivers. A Mixed-Effects Logistic Regression model was developed with probability of a SCE as the response variable and driver and work zone characteristics as predictor variables. The final model indicated glances over 1 second away from the driving task and following closely increased risk of an SCE by 3.8 times and 2.9 times, respectively. Average speed was negatively correlated to crash risk. This is counterintuitive since in most cases, it is expected that higher speeds are related to back of queue crashes. However, most queues form under congested conditions. As a result, vehicles encountering a back of queue would be more likely to be traveling at lower speeds.

Factors Associated with Closely Spaced Pregnancies at the Departmental Hospital Centre of Zou-Collines (Benin) in 2022

Introduction: Birth spacing to prevent closely-spaced pregnancies, which are a source of maternal and neonatal morbidity and mortality, is not always respected by our women, as recommended by the World Health Organization (WHO). The objective was to study the factors associated with closely-spaced pregnancies among women followed at the Departmental Hospital Center du Zou et du Colline (DHC/Z-C) in 2022. Study Method: This was a descriptive, analytical cross-sectional study with prospective data collection from 20 May to 30 June 2022. Results: At the end of the study, 83 of the 117 women surveyed had close pregnancies, a frequency of 70.94%. They had an average age of 27.0 ± 6.3 years and were of Fon ethnicity and related in 91.57% of cases. The majority were married (96.60%). The factors significantly associated with these closely spaced pregnancies were age between 25 and 30 years, household occupation, age at first birth, age at marriage, women’s perception of contraceptive method (CM), women’s knowledge of the existence of a center providing family planning services and non-use of MC. Conclusion: Close pregnancies are still very common in the commune of Abomey. It is therefore important to increase the use of contraceptive methods in this part of the country.

Study on the Effect of Behavioral Interventions Based on the Theory of“Knowledge,Attitude,Practice”on Tuberculosis Cognitive Level of Close Contacts

Objective:Based on the theory of“Knowledge,Attitude,Practice,”health education was carried out for close contact with tuberculosis(TB)at a university in Beijing,and its impact on the students’awareness of TB was studied.This study could provide a reference for effective health education interventions among close contacts of TB in undergraduates.Methods:102 undergraduates who had close contact with one confirmed case of pulmonary TB in April 2021 at a university were randomly divided into a control group and an intervention group.The intervention group was given the“Knowledge,Attitude,Practice”health education intervention by professionally trained doctors,and were asked to complete a self-designed TB-related questionnaire before and after the intervention.Both groups performed the Tuberculin Skin Test(TST)and chest X-ray(CR)examination.Results:The rate of the TST test was 18.6%,and CR examination showed 0 cases of pulmonary TB.The survey results showed that the TB awareness rate(96.1%,49/51)in the intervention group was significantly higher than that of the control group(51.0%,26/51),and the differences in awareness rate between the two groups were statistically significant(P<0.05).Regarding the attitude/skills toward TB,the prevalence rate of tuberculosis-related attitude/behavior in the intervention group(94.1%,47/51)was significantly higher than that of the control group(60.8%,31/51),and the differences between the two groups were statistically significant(P<0.05).Conclusion:Both self-controlled and parallel-controlled studies showed that the health education intervention of“Knowledge,Attitude,Practice”improved the awareness of TB among close contacts of TB in undergraduates.

如何理解“closing the barn door after the horse have escaped”?

然而,由教育部考试中心组织编写、高等教育出版社出版的《全国硕士研究生入学英语考试分析(2001年版)》对这道题的分析是:“……并且打个比喻说,马逃走了,关上马棚的大门,人们却发现骑在马上的正是这些受过教育的人他们自己。”显然,这样的分析对理解原文并不能起多少作用,读后考生仍然感到云里雾里。

Nonlinear model characterizing stress-strain relationship and permeability change of contact compression fracture at closing stage

Understanding the stress-strain relationship and permeability change for contact compression fracture at closing stage has been a hot issue for a long time.Previous investigations of this topic were mainly focused on experimental tests;however,theoretical approaches were rarely reported.Based on this,this paper focuses on the contact fracture at closing stage when rock is uniaxially loaded,and then a theoretical model is proposed.Based on the change of fracture elasticity modulus,it shows that as crack apertures are gradually reduced in the loading process,the permeability of rock sample will decrease progressively.This scenario shows that theoretical computation matches well with the experimental results.Finally,the effects of ratio of sample size to fracture aperture(n).pore pressure(P),and initial aperture(b) on stress-strain relationship and permeability change for contact compression fracture at closing stage are analyzed.

Experimental Study of the Multi-gap Multi-channel Gas Spark Closing Switch

A coaxial multi-gap multi-channel spark switch with stainless-steel-spring ring gap electrodes is designed and investigated. The switch is triggered by a pulse applied to the cylindrical electrode outside the discharging channel through a parasitic capacitance coupling. The jitter of the switch is reduced by several short-distance gas gaps in series, and its inductance is reduced by a multi-channel discharge on account of the inductance isolation between the coils of the spring ring electrode. The experimental results indicate that the switch is of low inductance (15-30 nH), low jitter (-3 ns), and stable breakdown performance