AIM: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats. METHODS: Twenty Wistar rats were divided ...AIM: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats. METHODS: Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol(5-12 g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis. RESULTS: Plasma endotoxin levels were increased more significantly in group E(129+/-21) ng x L(-1) and (187+/-35) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53+/-11) ng x L(-1), respectively (P【0.05). Mean values of plasma ALT levels were (1867+/-250) nkat x L(-1) and (2450+/-367) nkat x L(-1) in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (P【0.05). CONCLUSION: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.展开更多
目的:研究分化抗原簇14(cluster of differentiation 14,CD14)启动子基因多态性与青藏高原藏族人群胃癌易感性的相关性。方法:采用聚合酶链反应-连接酶反应(PCR-LDR)技术对186例藏族胃癌患者和216例藏族正常对照人群CD14启动子基因多态...目的:研究分化抗原簇14(cluster of differentiation 14,CD14)启动子基因多态性与青藏高原藏族人群胃癌易感性的相关性。方法:采用聚合酶链反应-连接酶反应(PCR-LDR)技术对186例藏族胃癌患者和216例藏族正常对照人群CD14启动子基因多态性进行分析,并就特定基因型与胃癌发病风险及生存进行分析。结果:在胃癌组和对照组中,CD14 rs5744455C>T位点的等位基因T的频率分别是40.8%和20.0%,基因型TT的频率分别是20.0%和1.8%,等位基T和基因型TT增加了胃癌的发病风险;而rs59568848 C>T位点的基因型CT频率分别是34.5%和74.5%,CT明显降低了胃癌的发病风险,胃癌组基因型TT和等位基因C、T的频率与对照组之间无明显差异。结论:CD14启动子区rs5744455 C/T等位基因T和基因型TT明显增加藏族人群胃癌的风险,而rs59568848C>T中基因型CT则明显减少胃癌的发生。展开更多
AIM: Crohn's disease(CD)and ulcerative colitis(UC)are multifactorial diseases with a significant genetic background.Apart from CARD15/NOD2 gene, evidence is accumulating that molecules related to the innate immune...AIM: Crohn's disease(CD)and ulcerative colitis(UC)are multifactorial diseases with a significant genetic background.Apart from CARD15/NOD2 gene, evidence is accumulating that molecules related to the innate immune response such as CD14 or Toll-like receptor 4 (TLR4), are involved in their pathogenesis. In further exploring the genetic background of these diseases, we investigated the variations in the CARD15/NOD2 gene (Arg702Trp,Gly908Arg and Leu1007fsinsC), and polymorphisms in the TLR4 gene (Asp299Gly and Thr399Ile) as well as in the promoter of the CD14 gene (T/C at position -159) in Greek patients with CD and UC.METHODS: DNA was obtained from 120 patients with CD,85 with UC and 100 healthy individuals. Genotyping was performed by allele specific PCR or by PCR-RFLP analysis.RESULTS: The 299Gly allele frequency of the TLR4 gene and the T allele and TT genotype frequendes of the CD14 promoter were significantly higher in CD patients only compared to healthy individuals (P = 0.026＜0.05; P = 0.0048＜0.01 and P= 0.047＜0.05 respectively). Concerning the NOD2/CARD15mutations the overall presence in CD patients was significantly higher than that in UC patients or in controls.Additionally, 51.67% of the CD patients were carriers of a TLR4 and/or CD14 polymorphic allele and at least one variant of the NOD2/CARD15, compared to 27% of the UC patients. It should be pointed out that both frequencies significantly increased as compared with the 10% frequency of multiple carriers found in healthy controls. A possible interaction of the NOD2/CARD15 with TLR4 and especially CD14, increased the risk of developing inflammatory bowel disease (IBD).CONCLUSION: Our results indicate that co-existence of a mutation in either the TLR4 or CD14 gene, and in NOD2/CARD15is associated with an increased susceptibility to developing CD compared to UC, and to developing either CD or UC compared to healthy individuals.展开更多
目的研究可溶性白细胞分化抗原14（soluble cluster of differentiation antigen14，sCD14）与损伤严重程度评分（injuryseverityscore，ISS）对急诊多发伤患者预后的相关性及判断价值。方法选择北京朝阳医院急诊外科2009年10月至2010年...目的研究可溶性白细胞分化抗原14（soluble cluster of differentiation antigen14，sCD14）与损伤严重程度评分（injuryseverityscore，ISS）对急诊多发伤患者预后的相关性及判断价值。方法选择北京朝阳医院急诊外科2009年10月至2010年3月首次就诊并且住院治疗的多发伤患者86例作为多发伤组，随访28d预后分为存活组和死亡组，另选20名健康体检者作为健康对照组。检测多发伤组就诊24h内以及第3、5、7天和健康对照组血清sCD14质量浓度并进行APACHEII评分，并在急诊24h内进行损伤严重程度评分并且根据预后计算与sCD14的相关性，并利用ROC曲线判断sCD14与两种不同评分方法对多发伤患者死亡的预测价值。结果多发伤组患者入院后sCD14质量浓度进行性上升，各时间点质量浓度均明显高于健康对照组（P〈0．01）；死亡组sCD14质量浓度各个时间点明显高于存活组（P〈0．05）；多发伤组患者sCD14质量浓度与损伤严重度评分呈正相关（r=0．469，P〈0．01）；ROC曲线分析，sCD14与ISS评分在预测多发伤患者死亡风险程度上均具有预测价值，但二者曲线下面积（sCD14=0．820，ISS评分0．705）差异无统计学意义（P〉0．05）。结论多发伤患者sCD14质量浓度与ISS评分具有正相关性，对多发伤患者病情预后具有预测意义。展开更多
基金Supported by the National Natural Science Foundation of China(No.39970719).
文摘AIM: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats. METHODS: Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). Group E was fed with ethanol(5-12 g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis. RESULTS: Plasma endotoxin levels were increased more significantly in group E(129+/-21) ng x L(-1) and (187+/-35) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53+/-11) ng x L(-1), respectively (P【0.05). Mean values of plasma ALT levels were (1867+/-250) nkat x L(-1) and (2450+/-367) nkat x L(-1) in Group E. The values were increased more dramatically in ethanol-fed rats than in Group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (P【0.05). CONCLUSION: Ethanol administration led to a significant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.
基金Supported by the EU Project "Sacrohn" N. QLK2-CT-2000-00928.
文摘AIM: Crohn's disease(CD)and ulcerative colitis(UC)are multifactorial diseases with a significant genetic background.Apart from CARD15/NOD2 gene, evidence is accumulating that molecules related to the innate immune response such as CD14 or Toll-like receptor 4 (TLR4), are involved in their pathogenesis. In further exploring the genetic background of these diseases, we investigated the variations in the CARD15/NOD2 gene (Arg702Trp,Gly908Arg and Leu1007fsinsC), and polymorphisms in the TLR4 gene (Asp299Gly and Thr399Ile) as well as in the promoter of the CD14 gene (T/C at position -159) in Greek patients with CD and UC.METHODS: DNA was obtained from 120 patients with CD,85 with UC and 100 healthy individuals. Genotyping was performed by allele specific PCR or by PCR-RFLP analysis.RESULTS: The 299Gly allele frequency of the TLR4 gene and the T allele and TT genotype frequendes of the CD14 promoter were significantly higher in CD patients only compared to healthy individuals (P = 0.026＜0.05; P = 0.0048＜0.01 and P= 0.047＜0.05 respectively). Concerning the NOD2/CARD15mutations the overall presence in CD patients was significantly higher than that in UC patients or in controls.Additionally, 51.67% of the CD patients were carriers of a TLR4 and/or CD14 polymorphic allele and at least one variant of the NOD2/CARD15, compared to 27% of the UC patients. It should be pointed out that both frequencies significantly increased as compared with the 10% frequency of multiple carriers found in healthy controls. A possible interaction of the NOD2/CARD15 with TLR4 and especially CD14, increased the risk of developing inflammatory bowel disease (IBD).CONCLUSION: Our results indicate that co-existence of a mutation in either the TLR4 or CD14 gene, and in NOD2/CARD15is associated with an increased susceptibility to developing CD compared to UC, and to developing either CD or UC compared to healthy individuals.
文摘目的研究可溶性白细胞分化抗原14（soluble cluster of differentiation antigen14，sCD14）与损伤严重程度评分（injuryseverityscore，ISS）对急诊多发伤患者预后的相关性及判断价值。方法选择北京朝阳医院急诊外科2009年10月至2010年3月首次就诊并且住院治疗的多发伤患者86例作为多发伤组，随访28d预后分为存活组和死亡组，另选20名健康体检者作为健康对照组。检测多发伤组就诊24h内以及第3、5、7天和健康对照组血清sCD14质量浓度并进行APACHEII评分，并在急诊24h内进行损伤严重程度评分并且根据预后计算与sCD14的相关性，并利用ROC曲线判断sCD14与两种不同评分方法对多发伤患者死亡的预测价值。结果多发伤组患者入院后sCD14质量浓度进行性上升，各时间点质量浓度均明显高于健康对照组（P〈0．01）；死亡组sCD14质量浓度各个时间点明显高于存活组（P〈0．05）；多发伤组患者sCD14质量浓度与损伤严重度评分呈正相关（r=0．469，P〈0．01）；ROC曲线分析，sCD14与ISS评分在预测多发伤患者死亡风险程度上均具有预测价值，但二者曲线下面积（sCD14=0．820，ISS评分0．705）差异无统计学意义（P〉0．05）。结论多发伤患者sCD14质量浓度与ISS评分具有正相关性，对多发伤患者病情预后具有预测意义。
