[Objective] The research aimed to construct maize cDNA library under the co-stress of drought,salt and alkali,and initially analyze the expression of gene which related to the cellular function.[Method] The total RNA ...[Objective] The research aimed to construct maize cDNA library under the co-stress of drought,salt and alkali,and initially analyze the expression of gene which related to the cellular function.[Method] The total RNA of mixed tissue（leaf,stem and blossom bud） of maize YQ7-96 in the male and female differentiation period（12-leaf age） was extracted.By using SMART technology,cDNA library of pDNR-LIB vector was constructed.BlastX contrast and MIPS classification analysis of EST sequence were carried out by randomly picking the clone.[Result] 3 027 cDNA clones were picked out to sequence.The length of 94.45% EST was bigger than 400 bp,and 1 861 single gene clusters were obtained.The gene which maintained the normal physiological activity occupied 65.36%.The genes which involved in the intracellular transportation,signal transduction,cell defense and cycle,DNA metabolic process were respectively 9.26%,6.58%,2.63% and 3.16%.[Conclusion] Based on the successful construction of maize cDNA library under the co-stress of drought,salt and alkali,EST was sequenced,analyzed and classified.EST which related to the cellular function was screened.It laid the foundation for the following research.展开更多
Objectives To investigate the effect of co-exposure of myocardial ischemia and cold stress on myocardial injury in rats and the relative mechanism.Methods Myocardial ischemia model was established by ligation of left ...Objectives To investigate the effect of co-exposure of myocardial ischemia and cold stress on myocardial injury in rats and the relative mechanism.Methods Myocardial ischemia model was established by ligation of left coronary artery.SD rats were randomly allocated to 4 groups; sham+normal temperature(S group),sham+cold stress(SC group),myocardial ischemia+ normal temperature(Ⅰgroup), myocardial ischemia+cold stress(IC group).On the condition of 26℃,SC and IC groups were keeped in a 4℃artificial chamber for 8h(8;00-16:00) for 4 consecu- tive days.Car diac function was assessed by echocardiography;pathological change was analyzed by HE staining;myocardial infarct size was determined by TTC staining;Bim,Caspase-3 expression in myocardium was determined by western blotting.Results It was demonstrated that co-exposure of myocardial ischemia and cold stress could significantly make the cardiac muscle in abnormal shape,increase the infarct size and the expression of Bim and Caspase-3.Conclusions Co-exposure of myocardial ischemia and cold stress may aggravate the cardiac injury,pro- apoptosis protein Bim is involved.展开更多
文摘[Objective] The research aimed to construct maize cDNA library under the co-stress of drought,salt and alkali,and initially analyze the expression of gene which related to the cellular function.[Method] The total RNA of mixed tissue（leaf,stem and blossom bud） of maize YQ7-96 in the male and female differentiation period（12-leaf age） was extracted.By using SMART technology,cDNA library of pDNR-LIB vector was constructed.BlastX contrast and MIPS classification analysis of EST sequence were carried out by randomly picking the clone.[Result] 3 027 cDNA clones were picked out to sequence.The length of 94.45% EST was bigger than 400 bp,and 1 861 single gene clusters were obtained.The gene which maintained the normal physiological activity occupied 65.36%.The genes which involved in the intracellular transportation,signal transduction,cell defense and cycle,DNA metabolic process were respectively 9.26%,6.58%,2.63% and 3.16%.[Conclusion] Based on the successful construction of maize cDNA library under the co-stress of drought,salt and alkali,EST was sequenced,analyzed and classified.EST which related to the cellular function was screened.It laid the foundation for the following research.
文摘Objectives To investigate the effect of co-exposure of myocardial ischemia and cold stress on myocardial injury in rats and the relative mechanism.Methods Myocardial ischemia model was established by ligation of left coronary artery.SD rats were randomly allocated to 4 groups; sham+normal temperature(S group),sham+cold stress(SC group),myocardial ischemia+ normal temperature(Ⅰgroup), myocardial ischemia+cold stress(IC group).On the condition of 26℃,SC and IC groups were keeped in a 4℃artificial chamber for 8h(8;00-16:00) for 4 consecu- tive days.Car diac function was assessed by echocardiography;pathological change was analyzed by HE staining;myocardial infarct size was determined by TTC staining;Bim,Caspase-3 expression in myocardium was determined by western blotting.Results It was demonstrated that co-exposure of myocardial ischemia and cold stress could significantly make the cardiac muscle in abnormal shape,increase the infarct size and the expression of Bim and Caspase-3.Conclusions Co-exposure of myocardial ischemia and cold stress may aggravate the cardiac injury,pro- apoptosis protein Bim is involved.
基金国家重点研发计划项目(2021YFD160014101-02)湖北省重点研发计划项目(2020BBB061)资助+1 种基金supported by the National Key Research and Development Program of China(2021YFD160014101-02)the National Key Research and Development Program of Hubei Province(2020BBB061)。
