The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rat...The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0, 451±0, 056, and 0. 390±0. 040 in pellets, the former was significantly higher than the later （n=20, p〈0, 01）, The average acrosomic integrity rate of the sperms was 0, 613±0. 049 in straws, and 0. 476±0. 057 in pellets. The former was significantly higher than the later （n= 20, p〈0.05）. The fertility rates （%） of sperms in pellets, straws and freshly colleered semen at the third day after artificial insemination （AI） were 85.32±2. 32, 87. 73±1.00 and 90.77±1.68, respectively. The fertility rate,s of the three types of semen at the fourth and fifth days were lower than the third day＇s. Variance analysts shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen （n=3, p〈0.01）, and the fertilities of the spermatozoa in straws were significantly higher than those in pellets （n= 3, p〈0.01）.展开更多
AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm c...AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.展开更多
文摘The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0, 451±0, 056, and 0. 390±0. 040 in pellets, the former was significantly higher than the later （n=20, p〈0, 01）, The average acrosomic integrity rate of the sperms was 0, 613±0. 049 in straws, and 0. 476±0. 057 in pellets. The former was significantly higher than the later （n= 20, p〈0.05）. The fertility rates （%） of sperms in pellets, straws and freshly colleered semen at the third day after artificial insemination （AI） were 85.32±2. 32, 87. 73±1.00 and 90.77±1.68, respectively. The fertility rate,s of the three types of semen at the fourth and fifth days were lower than the third day＇s. Variance analysts shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen （n=3, p〈0.01）, and the fertilities of the spermatozoa in straws were significantly higher than those in pellets （n= 3, p〈0.01）.
基金the Natural Science Foundation of Guangdong Province,No.940567the National Natural Science Foundation of China,No.39970374
文摘AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.
