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Application of Polar Code-Based Scheme in Cloud Secure Storage
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作者 Zhe Li Yiliang Han Yu Li 《国际计算机前沿大会会议论文集》 2020年第1期676-689,共14页
In view of the fact that the quantum computer attack is not considered in the cloud storage environment,this paper selects the code-based public key encryption scheme as the security protection measure in the cloud st... In view of the fact that the quantum computer attack is not considered in the cloud storage environment,this paper selects the code-based public key encryption scheme as the security protection measure in the cloud storage.Based on random linear code encryption scheme,it employs the structure of the RLCE scheme and Polar code polarization properties,using the Polar code as underlying encoding scheme,through the method of RLCEspad,putting forward a kind of improved public key encryption scheme which considers semantic security and is resistant to adaptively chosen ciphertext attacks.The improved scheme is applied to cloud storage to ensure that the storage environment will not be attacked by quantum computer while ensuring the confidentiality,availability and reliability. 展开更多
关键词 Post-quantum cryptography Polar code Random linear code encryption code-based scheme Cloud storage
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Structural Properties of the RNA Synthesized by Glutamate Dehydrogenase for the Degradation of Total RNA 被引量:3
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作者 Godson O. Osuji Paul M. Johnson 《Advances in Enzyme Research》 2018年第3期29-52,共24页
Glutamate dehydrogenase (GDH)-synthesized RNA, a nongenetic code-based RNA is suitable for unraveling the structural constraints imposed on the regulation (transcription, translation, siRNA etc.) of metabolism by gene... Glutamate dehydrogenase (GDH)-synthesized RNA, a nongenetic code-based RNA is suitable for unraveling the structural constraints imposed on the regulation (transcription, translation, siRNA etc.) of metabolism by genetic code. GDH-synthesized RNAs have been induced in whole plants to knock out target mRNA populations thereby producing plant phenotypes that are allergen-free;enriched in fatty acids, essential amino acids, shikimic acid, resveratrol etc. Methods applied hereunder for investigating the structural properties of GDH-synthesized RNA included purification of GDH isoenzymes, synthesis of RNA by the isoenzymes, reverse transcription of the RNA to cDNA, sequencing of the cDNA, computation of the G+C-contents, profiling the stability through PCR amplification compared with genetic code-based DNA;and biochemical characterization of the RNAs synthesized by individual hexameric isoenzymes of GDH. Single product bands resulted from the PCR amplification of the cDNAs of GDH-synthesized RNA, whereas several bands resulted from the amplification of genetic code-based DNA. The cDNAs have wide G+C-contents (35% to 59%), whereas genetic code-based DNA has narrower G+C-contents (50% to 60%). The GDH β6 homo-hexameric isoenzyme synthesized the A+U-rich RNAs, whereas the a6, and α6 homo-hexameric isoenzymes synthesized the G+C-rich RNAs. Therefore, the RNA synthesized by GDH is different from genetic code-based RNAs. In vitro chemical reactions revealed that GDH-synthesized RNA degraded total RNA to lower molecular weight products. Therefore, GDH-synthesized RNA is RNA enzyme. Dismantling of the structural constraints imposed on RNA by genetic code liberated RNA to become an enzyme with specificity to degrade unwanted transcripts. The RNA enzyme activity of GDH-synthesized RNA is ubiquitous in cells;it is readily induced by treatment of plants with mineral nutrients etc. and may simplify experimental approaches in plant enzymology and molecular biology research projects. 展开更多
关键词 RNA ENZYME Synthesis Nongenetic code-based RNA CLONING Vector DNA Touch-Down PCR Frame SHIFT Sequence HOMOLOGY
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Total RNA Degradation <i>in Vitro</i>and <i>in Vitro</i>by Glutamate Dehydrogenase-Synthesized RNA Enzyme: Biotechnological Applications 被引量:2
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作者 Godson O. Osuji Wenceslaus C. Madu Paul M. Johnson 《Advances in Bioscience and Biotechnology》 2019年第4期59-85,共27页
Glutamate dehydrogenase regulates crop development, growth, and biomass yield through its synthesis of non-genetic code-based RNA. Understanding the mechanism of GDH-synthesized RNA enzyme would enhance the agricultur... Glutamate dehydrogenase regulates crop development, growth, and biomass yield through its synthesis of non-genetic code-based RNA. Understanding the mechanism of GDH-synthesized RNA enzyme would enhance the agriculture innovation capacity of the more than a billion urban gardeners, smallholder, and limited resources indigenous farmers. Different metabolic variants were prepared by treating peanut growing on healthy soil with stoichiometric mixes of mineral salt solutions. Peanut GDH charge isomers were purified to homogeneity by electrophoresis, and made to synthesize RNA enzyme. Peanut total RNA was 5’-end labeled with [γ-32P]ATP and made to react as substrate in vitro with GDH-synthesized RNA from another metabolic variant of peanut. Agarose, and polyacrylamide gel electrophoresis of the reaction products showed that tRNA, rRNA, and most of the mRNAs were degraded to mononucleotides, but total RNAs that were not mixed with GDH-synthesized RNAs were not degraded. When the non-homologous sequence sections of the GDH-synthesized RNA were clipped out, the homologous sections failed to produce Northern bands with peanut total RNA. Therefore, the non-homologous sequence sections served to identify, position, and align the GDH-synthesized RNA to its target total RNA site independent of genetic code;the degradation of total RNA being via non-canonical base alignments in the enzyme-substrate complex, followed by electromagnetic destruction of the total RNA, the less stable of the two kinds of RNA. This is the science-based corner stone that buttresses the crop production efforts of limited resources farmers because GDH-synthesized RNAs quickly degrade superfluous total RNA of the crop in response to the soil mineral nutrient deficiencies thereby minimizing wastage of metabolic energy in the synthesis of unnecessary protein enzymes while optimizing biomass metabolism, crop growth, and maximum crop yields. In vitro hydrolysis of total RNA by GDH-synthesized RNA is the game changing, prototype, R&D methods for cleansing sick total RNA from cells, tissues, and whole organisms. 展开更多
关键词 Arachis HYPOGAEA Limited Resource Farmers Stoichiometric Salt Mixes GDH ISOENZYME Purification Nongenetic code-based RNA Electrophoresis DNA:RNA Hybrids
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Engineering Analysis of Strong Motion Data from Recent Earthquakes in Sichuan, China
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作者 HUANG Chen GALASSO Carmine 《工程科学与技术》 EI CAS CSCD 北大核心 2018年第3期112-124,共13页
Recent earthquakes in the Sichuan Province have contributed to significantly expand the existing ground-motion database for China with new,high-quality ground-motion records.This study investigated the compatibility o... Recent earthquakes in the Sichuan Province have contributed to significantly expand the existing ground-motion database for China with new,high-quality ground-motion records.This study investigated the compatibility of ground-motion prediction equations(GMPEs)established by the NGA-West2 project in the US and local GMPEs for China,with respect to magnitude scaling,distance scaling,and site scaling implied by recent Chinese strong-motion data.The NGA-West2 GMPEs for shallow crustal earthquakes in tectonically active regions are considerably more sophisticated than widely used previous models,particularly in China.Using a mixed-effects procedure,the study evaluated event terms(inter-event residuals)and intra-event residuals of Chinese data relative to the NGA-West2 GMPEs.Distance scaling was investigated by examining trends of intra-event residuals with source-to-site distance.Scaling with respect to site conditions was investigated by examining trends of intra-event residuals with soil type.The study also investigated other engineering characteristics of Chinese strong ground motions.In particular,the records were analyzed for evidence of pulse-like forward-directivity effects.The elastic median response spectra of the selected stations were compared to code-mandated design spectra for various mean return periods.Results showed that international and local GMPEs can be applied for seismic hazard analysis in Sichuan with minor modification of the regression coefficients related to the source-to-site distance and soil scaling.Specifically,the Chinese data attenuated faster than implied by the considered GMPEs and the differences were statistically significant in some cases.Near-source,pulse-like ground motions were identified at two recording stations for the 2008 Wenchuan earthquake,possibly implying rupture directivity.The median recorded spectra were consistent with the code-based spectra in terms of amplitude and shape.The new ground-motion data can be used to develop advanced ground-motion models for China and worldwide and,ultimately,for advancing probabilistic seismic hazard assessment(PSHA). 展开更多
关键词 ground motion prediction equations NGA-West2 project code-based spectrum pulse-like ground motions
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