BTB与CNC同源蛋白1通过调节MYC相关因子X二聚化蛋白1基因对套细胞淋巴瘤发生发展的影响

目的 探讨BTB与CNC同源蛋白1(BACH1)通过调节MYC相关因子X二聚化蛋白1(MXD1)基因对套细胞淋巴瘤(MCL)发生发展的影响。方法 采用靶向剪切及转座酶技术(CUT&Tag)检测BACH1下游靶基因,并通过序列比对分析寻找BACH1与MXD1的结合位点。通过双荧光素酶报告基因系统验证BACH1对MXD1的调控作用,并采用慢病毒技术构建BACH1过表达及敲低的稳转MCL细胞株,采用蛋白质印迹法(Western blot)检测MXD1蛋白表达水平。应用GSE16411数据集分析MCL细胞和正常B细胞中MXD1 m RNA相对表达量。应用GSE132929及GSE21452数据集筛选MXD1共表达基因,对MXD1共表达基因进行基因本体(GO)和京都基因与基因组百科全书(KEGG)信号通路富集分析。采用噻唑蓝(MTT)法检测MXD1正相关基因应激诱导磷蛋白1同源物含U-框蛋白1(STUB1)的特异性激活剂YL109处理后MCL细胞的活力。结果 BACH1能够靶向结合MXD1并抑制MXD1基因的启动子活性。BACH1敲低后MXD1蛋白表达水平升高,而BACH1过表达后MXD1蛋白表达水平降低。通过GSE16411数据集分析发现,MCL细胞中MXD1 mRNA相对表达量明显低于正常B细胞,差异有统计学意义(P﹤0.01)。在GSE132929数据集中筛选出2222个MXD1共表达基因,在GSE21452数据集中筛选出503个MXD1共表达基因。GO分析显示,MXD1共表达基因主要富集于信号转导、蛋白质磷酸化、细胞葡萄糖醛酸化、类黄酮葡萄糖醛酸化等代谢相关的生物学过程;KEGG信号通路分析显示,MXD1共表达基因主要富集于肝脏发育的代谢途径、蛋白质的消化和吸收、Janus激酶(JAK)/信号转导及转录激活因子(STAT)信号通路等。采用YL109处理MCL细胞后可显著降低细胞存活率。结论 BACH1通过结合MXD1近端启动子负向调控MXD1的表达水平,且BACH1介导的MXD1表达改变很可能通过调控代谢过程参与MCL的发生发展。

基于SKIP宏程序编程CNC无线测头在线智能检测系统设计

针对数控铣削加工精密零件尺寸精度测量时,因离线测量易产生测量误差、效率低、成本高等问题,通过赋值相关检测路径及补偿的参数,编写零件在线检测及误差补偿宏程序,开发了基于三菱M70数控系统跳过指令SKIP在线检测与误差补偿宏程序控制系统。应用结果表明:该系统在线检测重复定位精度能达到0.001~0.003 mm,能适用于精密零件数控铣削加工,具有一定的推广使用价值。

CNC机床丝杠热误差实时补偿设计及自动补偿试验

为了降低数控机床丝杠传动系统因热误差引起的定位误差,完成机床工作台各运行阶段的实时补偿。用非接触模式为丝杠非电机连接端安装位移检测器,以电涡流传感器时间监测丝杠端面位置数据,获得丝杠产生的总热误差,通过热补偿得到每段丝杠的热误差程度,确定每段坐标系原点发生的偏移,完成自主补偿机床丝杠热误差的效果。研究结果表明:采用分段补偿方法可以获得比其他热误差补偿模型更优的丝杠全段补偿性能。分别检测丝杠各段发生的热误差再对其实施补偿,可以根据丝杠各部位热误差程度实施补偿。

Molecular Dynamics, Diffusion Coefficients and Activation Energy of the Electrolyte (Anode) in Lithium (Li and Li+), Sodium (Na and Na+) and Potassium (K and K+)

This work is a simulation modelling with the LAMMPS calculation code of an electrode based on alkali metals (lithium, sodium and potassium) using the MEAM potential. For different multiplicities, two models were studied;with and without gap. In this work, we present the structural, physical and chemical properties of the lithium, sodium and potassium electrodes. For the structural properties, the cohesive energy and the mesh parameters were calculated, revealing that, whatever the chemical element selected, the compact hexagonal hcp structure is the most stable, followed by the face-centred cubic CFC structure, and finally the BCC structure. The most stable structure is lithium, with a cohesion energy of -6570 eV, and the lowest bcc-hcp transition energy of -0.553 eV/atom, followed by sodium. For physical properties, kinetic and potential energies were calculated for each of the sectioned chemical elements, with lithium achieving the highest value. Finally, for the chemical properties, we studied the diffusion coefficient and the activation energy. Only potassium followed an opposite order to the other two, with the quantities with lacunae being greater than those without lacunae, whatever the multiplicity. The order of magnitude of the diffusion coefficients is given by the relationship DLi > DNa > Dk for the multiplicity 6*6*6, while for the activation energy the order is reversed.

Adaptive Random Effects/Coefficients Modeling

Adaptive fractional polynomial modeling of general correlated outcomes is formulated to address nonlinearity in means, variances/dispersions, and correlations. Means and variances/dispersions are modeled using generalized linear models in fixed effects/coefficients. Correlations are modeled using random effects/coefficients. Nonlinearity is addressed using power transforms of primary (untransformed) predictors. Parameter estimation is based on extended linear mixed modeling generalizing both generalized estimating equations and linear mixed modeling. Models are evaluated using likelihood cross-validation (LCV) scores and are generated adaptively using a heuristic search controlled by LCV scores. Cases covered include linear, Poisson, logistic, exponential, and discrete regression of correlated continuous, count/rate, dichotomous, positive continuous, and discrete numeric outcomes treated as normally, Poisson, Bernoulli, exponentially, and discrete numerically distributed, respectively. Example analyses are also generated for these five cases to compare adaptive random effects/coefficients modeling of correlated outcomes to previously developed adaptive modeling based on directly specified covariance structures. Adaptive random effects/coefficients modeling substantially outperforms direct covariance modeling in the linear, exponential, and discrete regression example analyses. It generates equivalent results in the logistic regression example analyses and it is substantially outperformed in the Poisson regression case. Random effects/coefficients modeling of correlated outcomes can provide substantial improvements in model selection compared to directly specified covariance modeling. However, directly specified covariance modeling can generate competitive or substantially better results in some cases while usually requiring less computation time.

不同CNC系统中变螺距螺纹的切削技巧

通过变螺距螺纹的机理分析与不同CNC系统中变螺距螺纹切削指令的介绍,从图样分析、坐标系建立、刀具选用和程序编制等方面,讲述槽等宽牙变距螺杆与牙等宽槽变距螺杆在FANUC、SINUMERIK数控系统机床上的加工。

Thermomechanical Dynamics (TMD) and Bifurcation-Integration Solutions in Nonlinear Differential Equations with Time-Dependent Coefficients

The new independent solutions of the nonlinear differential equation with time-dependent coefficients (NDE-TC) are discussed, for the first time, by employing experimental device called a drinking bird whose simple back-and-forth motion develops into water drinking motion. The solution to a drinking bird equation of motion manifests itself the transition from thermodynamic equilibrium to nonequilibrium irreversible states. The independent solution signifying a nonequilibrium thermal state seems to be constructed as if two independent bifurcation solutions are synthesized, and so, the solution is tentatively termed as the bifurcation-integration solution. The bifurcation-integration solution expresses the transition from mechanical and thermodynamic equilibrium to a nonequilibrium irreversible state, which is explicitly shown by the nonlinear differential equation with time-dependent coefficients (NDE-TC). The analysis established a new theoretical approach to nonequilibrium irreversible states, thermomechanical dynamics (TMD). The TMD method enables one to obtain thermodynamically consistent and time-dependent progresses of thermodynamic quantities, by employing the bifurcation-integration solutions of NDE-TC. We hope that the basic properties of bifurcation-integration solutions will be studied and investigated further in mathematics, physics, chemistry and nonlinear sciences in general.

TwinCAT CNC助力精密加工小型零部件

大型零部件的精密加工绝非易事。如何才能以微米级的精度大批量生产钟表中使用的齿轮和螺丝或者连接器接触引脚等零件?瑞士Esco和Affolter公司通过为其数控机床配备倍福的TwinCATCNC和伺服驱动技术实现了这一目标。

TwinCAT CNC助力精密加工小型零部件

开放、灵活直达核心需求大型零部件的精密加工绝非易事。如何才能以微米级的精度大批量生产钟表中使用的齿轮和螺丝或者连接器接触引脚等零件?瑞士Esco和Affolter公司通过为其数控机床配备倍福的TwinCAT CNC和伺服驱动技术实现了这一目标。

基于最小二乘法CNC无线测头智能检测控制系统设计

为提高CNC在机检测效率和质量,针对现有的接触式测头各种检测误差,研究一种基于最小二乘法曲线拟合插值方法的测量误差补偿模型,该算法通过最小二乘法分别对测头模型标定、系统测量误差补偿模型构建,设计了一套精度较高的CNC无线测头智能检测与控制系统,达到在机测量零件尺寸精度和误差补偿。生产结果证明该系统误差补偿算法的可行性、有效性与优越性较高,提高了生产效率、生产质量,降低了次品率、生产成本,能广泛适用于CNC数控铣削精密加工零件尺寸测量与误差补偿。

Impacts of Surface Exchange Coefficients on Simulations of Super Typhoon Megi(2010)Using a Coupled Ocean-Atmosphere-Wave Model

In this study,the effects of surface exchange coefficients on simulations of Super Typhoon Megi(2010)are investigated using a fully coupled ocean-atmosphere-wave model.Several experiments are conducted using different parameterization schemes for the drag(C_(D))and enthalpy exchange(C_(K))coefficients.For the selected case,considering only the leveling-off of C_(D)at high wind speeds does not effectively improve the simulated typhoon track,intensity,or size.We found that increasing C_(K)monotonically with wind speed(Komori et al.,2018)yields stronger winds and deeper pressures by enhancing latent and sensible heat fluxes,but typhoon intensity remains underestimated.We propose a new higher C_(K)than that from Komori et al.(2018)based on the theory of Emanuel(1995).This approach produces a greater modeled typhoon intensity that is in good agreement with the best track data and effectively improves the track error for the simulation.Improved accuracy for modeled typhoon intensity is achieved with the new coefficient because C_(K)/C_(D)reaches the threshold of about 0.75 predicted by Emanuel(1995).The new proposed C_(K)also results in a reasonably accurate modeled sea surface temperature.However,typhoon size and surface wave height are overestimated.This finding implies that more numerical tests for tropical cyclones of different nature(such as strong,weak,dissipating,rapidly intensifying,or weakening tropical cyclones)should be studied,and more physical processes should be explored in future coupled models.

Surface-functionalized cellulose nanocrystals(CNC)and synergisms with surfactant for enhanced oil recovery in low-permeability reservoirs

Nanocellulose,a natural polymeric nanomaterial,has attracted significant attention in enhanced oil recovery(EOR)applications due to its abundance,nanoscale,high oil-water interfacial adsorption ef-ficiency.In this study,surface-functionalized cellulose nanocrystals(SF-CNCs)were prepared via hy-drochloric acid hydrolysis and chemical modification,with adaptable nanosize and considerable dispersion stability in low-permeability reservoirs.The SF-CNCs were structurally characterized by FT-IR,Cryo-TEM,which have a diameter of 5-10 nm and a length of 100-200 nm.The SF-CNC dispersions possessed higher stability and stronger salt-tolerance than those of corresponding CNC dispersions,due to the strong hydrophilicity of the sulfonic acid group.It was synergistically used with a non-ionic surfactant(APG1214)to formulate a combined flooding system(0.1 wt%SF-CNC+0.2 wt%APG1214).The combined flooding system exhibits strong emulsification stability,low oil-water interfacial tension of o.03 mN/m,and the ability to alter the wettability for oil-wetting rocks.Furthermore,the combined system was_able to provide an optimum EOR efficiency of 20.2%in low-permeability cores with 30.13×10^(-3)μm^(2).Notably.it can enlarge the sweep volume and increase the displacement efficiency simultaneously.Overall,the newly formulated nanocellulose/surfactant combined system exhibits a remarkable EoR performance in low-permeability reservoirs.

THE FEKETE-SZEG?INEQUALITY AND SUCCESSIVE COEFFICIENTS DIFFERENCE FOR A SUBCLASS OF CLOSE-TO-STARLIKE MAPPINGS IN COMPLEX BANACH SPACES

Let C be the familiar class of normalized close-to-convex functions in the unit disk.In[17],Koepf demonstrated that,as to a function■in the class C,■By applying this inequality,it can be proven that‖a3|-|a2‖≤1 for close-to-convex functions.Now we generalized the above conclusions to a subclass of close-to-starlike mappings defined on the unit ball of a complex Banach space.

Theoretical calculations on Landé g-factors and quadratic Zeeman shift coefficients of nsnp ^(3)P_(0)^(o) clock states in Mg and Cd optical lattice clocks

The study of magnetic field effects on the clock transition of Mg and Cd optical lattice clocks is scarce.In this work,the hyperfine-induced Landég-factors and quadratic Zeeman shift coefficients of the nsnp ^(3)P_(0)^(o) clock states for ^(111,113)Cd and ^(25)Mg were calculated by using the multi-configuration Dirac–Hartree–Fock theory.To obtain accurate values of these parameters,the impact of electron correlations and furthermore the Breit interaction and quantum electrodynamical effects on the Zeeman and hyperfine interaction matrix elements,and energy separations were investigated in detail.We also estimated the contributions from perturbing states to the Landég-factors and quadratic Zeeman shift coefficients concerned so as to truncate the summation over the perturbing states without loss of accuracy.Our calculations provide important data for estimating the first-and second-order Zeeman shifts of the clock transition for the Cd and Mg optical lattice clocks.

Darboux transformation,infinite conservation laws,and exact solutions for the nonlocal Hirota equation with variable coefficients

This article presents the construction of a nonlocal Hirota equation with variable coefficients and its Darboux transformation.Using zero-seed solutions,1-soliton and 2-soliton solutions of the equation are constructed through the Darboux transformation,along with the expression for N-soliton solutions.Influence of coefficients that are taken as a function of time instead of a constant,i.e.,coefficient functionδ(t),on the solutions is investigated by choosing the coefficient functionδ(t),and the dynamics of the solutions are analyzed.This article utilizes the Lax pair to construct infinite conservation laws and extends it to nonlocal equations.The study of infinite conservation laws for nonlocal equations holds significant implications for the integrability of nonlocal equations.

Nondegenerate solitons of the(2+1)-dimensional coupled nonlinear Schrodinger equations with variable coefficients in nonlinear optical fibers

We derive the multi-hump nondegenerate solitons for the(2+1)-dimensional coupled nonlinear Schrodinger equations with propagation distance dependent diffraction,nonlinearity and gain(loss)using the developing Hirota bilinear method,and analyze the dynamical behaviors of these nondegenerate solitons.The results show that the shapes of the nondegenerate solitons are controllable by selecting different wave numbers,varying diffraction and nonlinearity parameters.In addition,when all the variable coefficients are chosen to be constant,the solutions obtained in this study reduce to the shape-preserving nondegenerate solitons.Finally,it is found that the nondegenerate two-soliton solutions can be bounded to form a double-hump two-soliton molecule after making the velocity of one double-hump soliton resonate with that of the other one.

基于NUC970系列芯片的CNC控制器设计与实现

当前市面上多数主流CNC控制器仍然沿用三星S3C2440+FPGA硬件设计架构,而此款芯片早在2010年就已停产,市面上现有的S3C2440A芯片大多数是拆机翻新品。因此,基于S3C2440A芯片的CNC控制器迫切需要使用新一代量产芯片进行升级换代。选用新塘科技NUC970系列芯片来替代现有的S3C2440A芯片,详细介绍了基于NUC970芯片的CNC控制器的设计与实现方法以及CNC控制器的硬件原理和控制软件流程,同时根据该芯片的性能特点优化了CNC控制器的ARM+FPGA硬件平台。

CNC自动化攻牙技术研究与应用

为适应目前模具行业的发展重要,必须不断地对模具加工方法进行优化改善,提升模具整体质量的同时缩短制造周期降低加工成本,保证在与同行业竞争中立于不败之地。在多数模具加工厂加工螺牙基本都采用摇臂钻加工,但摇臂钻加工出来螺牙无法保证螺牙垂直度从而影响螺牙质量,使用摇臂钻攻牙增加零件流转工序延长加工周期。为保证螺牙质量以及缩短加工周期,提出了用CNC机床通过程序加工进行自动化攻牙,在利用CNC机床加工零件的其余特征时同时进行攻牙加工。

Review of All Possible Conversion Equations of Influence Coefficients with Different Formats on Two-Plane Balancing

This article lists all possible conversion equations of influence coefficients(ICs)with different formats when some of these are known to handle two-plane balancing tasks with no or less trial weight runs.There are two approaches for two-plane balancing.One can treat it as a multi-plane balance problem involving a 2×2 matrix of complex ICs where two direct ICs along with two cross-effect ICs are generated so that correction weights at one or two balance planes can be determined.One can also apply a static pare(in-phase)and/or couple pair(180 degrees out-of-phase)weights for balancing.The latter approach has been used quite often in the field,especially on steam turbine and generator rotors.Dependent on vibration mode shapes and combinations as well as balance plane accessibility,sometimes applying static or couple pair weights can be a wise choice;other times weights at one or two end planes are needed.There are totally 4 possible sets of IC data due to weights at plane 1,plane 2,static pair,and couple pair.IC data would typically be obtained by applying trial weights followed by trial weight runs.It is found,however,that all these IC data can be converted easily without trial weight runs once any two of 4 sets are known.The above findings and conversion equations have been obtained analytically and verified by experimental results.Real cases are given to demonstratetheirapplications.

俄罗斯甲醇装置EPC合同授予CNCEC

近日,日本永旺集团(AEON)子公司GTM位于俄罗斯伏尔加格勒Khimprom经济特区甲醇装置的工程、采购和施工管理(EPC)合同授予中国化学工程集团有限公司(CNCEC)。该装置甲醇产能1 Mt/a,预计总投资800亿卢布(折合63.47亿元人民币),将采用丹麦托普索公司(Haldor Topsoe)SynCorTM技术,以天然气为原料生产甲醇。其中托普索将提供甲醇生产技术许可、基础工程、催化剂和专有设备,三菱重工将负责项目的前端工程设计。该项目将促进地区经济发展。