The Popularization and Application of Cold Storage Red Blood Cells or Whole Blood at -80 ℃ of the Rh(D) Negative Patients in Surgical Operation

Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation.

Pulsed cold plasma-induced blood coagulation and its pilot application in stanching bleeding during rat hepatectomy

This paper presents plasma-induced blood coagulation and its pilot application in rat hepatectomy by using a home-made pulsed cold plasma jet. Experiments were conducted on blood coagulation in vitro, the influence of plasma on tissue in vivo, and the pilot application of rat hepatectomy. Experimental results show that the cold plasma can lead to rapid blood coagulation. Compared with the control sample, the plasma-induced agglomerated layer of blood is thicker and denser, and is mostly composed of broken platelets. When the surface of the liver was treated by plasma, the influence of the plasma can penetrate into the liver to a depth of about 500 μm. During the rat hepatectomy, cold plasma was proved to be effective for stanching bleeding on incision. No obvious bleeding was found in the abdominal cavities of all six rats 48 h after the hepatectomy. This implies that cold plasma can be an effective modality to control bleeding during surgical operation.

Effects of a Traditional Japanese Medicine Goshajinkigan, Tokishigyakukagoshuyushokyoto on the Warm and Cold Sense Threshold and Peripheral Blood Flow

The purpose of this study was to investigate the effects of a traditional Japanese medicine Goshajinkigan (TJ-107) and Tokishigyakukagoshuyushokyoto (TJ-38) on warm sense threshold, cold sense threshold and the peripheral blood flow. 31 healthy volunteers (control group: 9people, TJ-107 group: 12 people, TJ-38group:10 people) were examined. Drugs administered 2.5 g a dose. Analysis was before and after 1 hour dosage. The warm and cold sense threshold in the thenar of the non-handedness site of these subjects was measured using a thermostimulator (Intercross-200, Intercross Co., Tokyo, Japan). The peripheral blood flow in the finger of the non-handedness site of these subjects was measured using a full-field laser perfusion imager (FLPI, Moor Instruments Ltd., England). Control: The vehicle had no significant effect on the warm sense threshold, cold sense threshold and the peripheral blood flow. TJ-107: The warm sense threshold and cold sense threshold were significantly decreased, and the reaction latency of cold sense was significantly shortening. The peripheral blood flow was significantly increased second and third finger at 115.6%, 119.3%, respectively. TJ-38: The cold sense threshold and the reaction latency of cold sense were significantly increased. The peripheral blood flow was significantly increased second and third finger with 114.3%, 112.8%, respectively. These results suggest that TJ-107 and TJ-38 have effects on the changed warm sense threshold, cold sense threshold and increased peripheral blood flow.

Effects of Bacillus subtilis Var. Natto Products on Capillary Blood Flow in Healthy Subjects with Peripheral Coldness: A Double-Blind, Placebo-Controlled, Randomized Parallel Study

Purpose: NKCP®, a natto-derived dietary food supplement whose main component is bacillopeptidase F produced by Bacillus subtilis var. natto, has antithrombotic, fibrinolytic, and pressure-lowering effects, and also is suggested to improve peripheral coldness. However, existing data are based on subjective evaluations with no scientific basis about the effects on peripheral coldness. Therefore, we aimed to investigate the effectiveness of NKCP®for peripheral coldness by measuring changes in blood flow using a laser doppler rheometer and biochemical indices. Patients and Methods: This was a double-blind, randomized, controlled study of individuals aged 30 - 70 years who complained of subjective symptoms of cold hands and feet. They were randomly divided into the NKCP®group and the placebo group to receive NKCP®250 mg once daily and dextrin 250 mg as placebo once daily, respectively. The experiment lasted 8 weeks, with an intervention period of 4 weeks and a washout period of 4 weeks. Results: One-month intake of NKCP®significantly increased blood flow rate for 1 min between 4 and 5 minutes after the end of cold loading compared to that before feeding (p = 0.038). Also, analysis of the 5-minute blood flow rate before and after 4 weeks of feeding showed a significant improvement in the NKCP®group (p = 0.007), although there was no significant difference in the placebo group (p = 0.215). Furthermore, the 5-minute blood flow at 4 weeks after the end of feeding was significantly improved compared to that before feeding in the NKCP®group (p = 0.049). Therefore, the effect continued for at least 1 month after discontinuation of administration. Conclusions: It is possible that NKCP®intake effectively improves blood flow in subjects with peripheral coldness. Therefore, continuous intake of NKCP®is expected to reduce the symptoms of peripheral coldness. In the future, it needs to investigate whether the effect of increasing blood flow after ingestion of NKCP®is effective in improving the symptoms of peripheral coldness.

武权生基于周期疗法治疗寒凝血瘀型子宫腺肌症痛经临床经验

介绍武权生教授运用周期疗法治疗寒凝血瘀型子宫腺肌症痛经的临床经验。武权生教授认为该病本质在于寒凝经脉胞宫致使气血运行不畅,其病机为寒凝阻滞、血瘀于内、日久成癥,治疗应当结合女性月经周期阴阳消长规律,结合中医周期疗法,分期论治。经期予以少腹逐瘀汤祛瘀散寒、养血通经以治标;经后期予以温肾暖宫方补气养血、温肾健脾以固本,辨证施治,达到标本兼治之效。本文将结合一则临床医案以探讨武教授运用周期疗法治疗寒凝血瘀型子宫腺肌症痛经的临床经验。

“国医楷模”陈景河辨治寒地慢性头痛经验

“国医楷模”陈景河先生认为寒地病邪致病具有寒邪重、风邪盛、痰湿蕴结、瘀血相兼的特点,因此,寒地慢性头痛的病因病机主要与风寒或风热伏邪、气血凝滞、虚、痰相关。治疗寒地慢性头痛当明三因之宜忌、审机制变。伏邪滞络者,治当辨别寒热,以宣散之法祛伏邪外出;气血凝滞者,治当活血通络,气血兼理;气血不足者,治当补虚而防壅,少佐宣药;痰浊阻络者,治以调气清热为本。附验案1则。

Effects of Warm Blood Cardioplegic Solution on Myocardial Protection

To evaluate the effects of warm blood cardioplegic solution on myocardial protection, normothermic induction and terminal perfusion of oxygenated blood cardioplegia in combination with intermittent administration of cold blood cardioplegia during ischemia were studied in an isolated working rat heart model.The experimental protocol consisted of a 120 min cardioplegic arrest followed by 45 min normothermic reperfusion. Myocardial content of adenosine triphosphate (ATP), recovery of the left ventricular function, release of creatine phosphokinase (CPK) and ultrastructure of myocardium were assessed before and after ischemia. The results showed that the hearts preserved with warm blood cardioplegic induction and terminal perfusion had significantly higher levels of ATP,better recovery of cardiac function and lower releases of CPK than those receiving cold blood cardioplegia alone, with myocardial tissue being of generally normal structure. These findings suggest that warm induction and terminal perfusion of blood cardioplegic solution can accelerate myocardial metabolic and functional recovery, preserve high-energy phosphate, reduce myocardial injury and enhance myocardial protection.

基于NLRP3/NF-κB/COX-2信号通路探讨痛经胶囊对原发性痛经大鼠的影响

目的:探讨痛经胶囊对原发性痛经大鼠NLRP3/NF-κB/COX-2信号通路的影响及相关作用机制。方法:选取成年健康清洁级SD雌性未孕且未交配大鼠60只,均处于动情间期。对实验动物进行分组处理,一共分为六组,每组分配动物10只,分组方法为随机数字表法,所分研究小组分别为:正常对照组、模型组、布洛芬胶囊组、痛经胶囊高剂量组、痛经胶囊中剂量组、痛经胶囊低剂量组。除空白组外,其余组建立寒凝血瘀痛经模型。正常对照组每日灌胃蒸馏水(1 ml/100 mg),模型组给予蒸馏水(1 ml/100 g)灌胃,痛经胶囊低、中、高剂量给予相应剂量灌胃,布洛芬胶囊组给予布洛芬胶囊(0.06 g/kg)灌胃,连续灌胃7 d。观察各组大鼠扭体反应次数;苏木精-伊红(HE)染色观察子宫病理情况;酶联免疫吸附测定法(ELISA)检测前列腺素F2α(PGF2α)、前列腺素F2(PGF2)含量;蛋白免疫印迹法检测大鼠子宫组织NLRP3、NF-κB、COX-2蛋白表达;实时荧光定量PCR(RT-PCR)检测大鼠子宫组织NLRP3、NF-κB、COX-2 mRNA表达情况。结果:与对照组相比,模型组大鼠扭体次数、PGF2α、OT水平增加(P<0.001),PGE2水平下降(P<0.001),子宫腺轻度扩张,可见粒细胞、淋巴细胞浸润,子宫组织中COX-2、NF-κB、NLRP3蛋白相对表达量明显上调(P<0.001),COX-2、NF-κB、NLRP3 mRNA水平明显上调(P<0.001);与模型组相比,各治疗组大鼠扭体次数、PGF2α、OT水平显著降低(P<0.05,P<0.01或P<0.001),PGE2水平显著上升(P<0.05,P<0.01或P<0.001),子宫结构清晰,内膜完整,无明显腺体肥大、子宫肌层增生,炎性浸润减少,COX-2、NF-κB、NLRP3蛋白相对表达量下调(P<0.05,P<0.01或P<0.001),COX-2、NF-κB、NLRP 3 mRNA水平下调(P<0.05,P<0.01或P<0.001)。结论:痛经胶囊可以改善子宫炎症细胞浸润的情况,通过调控NLRP3/NF-κB/COX-2信号通路调节痛经大鼠炎症反应从而起到镇痛作用。

COLD-PCR-HRM检测结直肠癌患者外周血KRAS基因突变

目的评价COLD-PCR-HRM检测结直肠癌患者外周血KRAS基因突变的临床应用价值。方法将已知突变型KRAS组织DNA与野生型DNA做系列稀释,突变DNA所占比例分别为50%、25%、10%、5%、3%、2%和1%。分别应用常规PCR-HRM法和COLD-PCR-HRM法对不同比例KRAS突变DNA样本进行检测,验证两种检测方法的灵敏度;同时应用COLD-PCR-HRM法对62例外周血和肿瘤组织配对样本进行一致性验证。结果 PCR-HRM法最低检测浓度为3%;COLD-PCR-HRM法最低检测浓度为1%,两种方法的检测灵敏度均明显高于直接测序法(P<0.05)。应用COLD-PCRHRM法检测两种样本中KRAS基因突变状态,其中血清样本中检测出13例突变(突变率21.0%,13/62),组织中检测出12例突变(突变率19.4%,12/62),两种标本同时存在突变的有9例,以组织中检测的KRAS状态为准,两者突变一致性为75.0%(9/12)。KRAS基因突变在组织与外周血中存在一致性(κ=0.649;P<0.001)。结论 COLD-PCR结合HRM明显提高了KRAS突变检测灵敏度,为其应用于低丰度突变样本检测提供了有利条件。

基于GnRH及其受体介导下丘脑-垂体-卵巢轴探讨温和灸干预妇科寒凝血瘀证作用机制

目的观察温和灸“气海”“关元”“中极”对妇科寒凝血瘀证模型大鼠促性腺激素释放激素(GnRH)及其受体的影响,探讨艾灸干预妇科寒凝血瘀证生殖功能的作用机制。方法将48只雌性SD大鼠随机分为空白组、模型组、艾灸组、抑制剂组、抑制剂+艾灸组和激动剂组,每组8只。采用冰水浴+肾上腺素注射法制备妇科寒凝血瘀证模型,各干预组造模同时分别予GnRH抑制剂/激动剂注射及温和灸干预。对大鼠进行症状评分,激光散斑检测在体子宫微循环血流灌注量,HE染色观察卵巢、子宫组织形态,ELISA检测血清生殖激素GnRH、卵泡刺激素(FSH)、黄体生成素(LH)及神经递质多巴胺(DA)、γ-氨基丁酸(GABA)含量,免疫组化染色、RT-qPCR、Western blot检测卵巢、子宫组织GnRH受体(GnRHR)、FSH受体(FSHR)、LH受体(LHR)mRNA和蛋白表达。结果与空白组比较,模型组大鼠症状评分明显升高,子宫微循环血流灌注量减少,卵泡减少,子宫内膜上皮变薄、结构混乱;血清GnRH、FSH、LH、GABA含量降低,DA含量升高;卵巢组织GnRHR、FSHR、LHR mRNA和蛋白表达下降(P<0.01)。与模型组比较,各干预组大鼠症状评分降低,子宫微循环血流灌注量增加,卵泡增多,子宫内膜增厚、腺体增多;血清GnRH、FSH、LH、GABA含量升高,DA含量降低;卵巢组织GnRHR、FSHR、LHR mRNA和蛋白表达升高(P<0.05,P<0.01)。结论艾灸可通过调节神经递质调控GnRH及其受体,改善下丘脑-垂体-卵巢轴功能,从而改善妇科寒凝血瘀证模型大鼠一般状态,提高生殖功能。

温经活血汤联合布洛芬治疗原发性痛经临床研究

目的:观察温经活血汤联合布洛芬治疗原发性痛经的效果及对前列腺素F2α(PGF2α)、内皮素-1(ET-1)的影响。方法:选取2021年3月—2023年3月苍南县中医院收治的80例原发性痛经患者作为研究对象,按随机数字表法分为中医组和西医组各40例。西医组给予布洛芬治疗,中医组在西医组的基础上给予温经活血汤治疗,2组均持续治疗6个月经周期。评价2组临床疗效,比较2组治疗前后中医证候评分、PGF2α、ET-1水平及生活质量评分,统计不良反应发生情况。结果:2组总有效率比较,差异无统计学意义(P>0.05);中医组愈显率高于西医组(P<0.05)。治疗后,2组各项中医证候评分较治疗前降低(P<0.05),且中医组各项中医证候评分低于西医组(P<0.05)。治疗后,2组PGF2α、ET-1水平较治疗前降低(P<0.05),且中医组PGF2α、ET-1水平低于西医组(P<0.05)。治疗后,2组生存质量量表(WHOQOLBREF)各维度评分较治疗前升高(P<0.05),且中医组WHOQOL-BRFF量表各维度评分高于西医组(P<0.05)。2组均未出现恶心、呕吐、皮疹、头晕等不良反应。结论:温经活血汤联合布洛芬能够有效缓解原发性痛经患者的临床症状,降低患者疼痛程度,从而提高患者生活质量。

自身抗-B伴冷凝集素患者输血前检测及输血治疗——附1例报告

目的对1例自身抗-B伴冷凝集素患者行血清学鉴定及输血疗效评估。方法37℃生理盐水洗涤患者红细胞并用巯基试剂处理,采用试管法、微柱凝胶法检测ABO血型抗原。用O型红细胞4℃吸收EDTA抗凝血浆去除冷凝集素,再用B型红细胞4℃吸收放散检测相关血型抗体,利用输血前后血红蛋白变化评估患者输血效果,并持续监测其ABO血型。结果血清学实验正定型检出B抗原,O型红细胞吸收放散检出冷凝集素,B型红细胞吸收放散检出患者血清中含有IgM抗-B。输注3 U O型洗涤红细胞无不良反应且输注有效。2月后随访患者,血型鉴定正反一致,均为B型。结论结合患者既往血型鉴定结果,本次血清学鉴定及后续追踪综合分析,患者ABO血型为B型,但存在一过性高效价冷凝集素和B抗原特异性自身抗体。

免疫治疗对晚期胃癌中医证型演变规律的影响

目的:观察免疫治疗对晚期胃癌(gastric cancer, GC)患者中医证型演变规律的影响。方法:共纳入58例晚期GC患者,其中腹膜转移组38例,无腹膜转移组20例,收集患者的一般资料、临床病理学特征及治疗前后中医证型。运用χ~2检验评估各项指标间的相关性,运用Kaplan-Meier法评估各种证型无进展生存期(progression-free survival, PFS)。结果:58例GC患者治疗前中医证型以痰湿凝结证、肝胃不和证为主,两种证型PFS比较,差异无统计学意义(P>0.05);治疗后以气阴两虚证、脾胃虚寒证为主,两种证型PFS比较,差异无统计学意义(P>0.05)。腹膜转移组治疗前主要证型为肝胃不和证、痰湿凝结证、气滞血瘀证;治疗后主要证型为脾胃虚寒证、气阴两虚证、气血亏虚证。无腹膜转移组治疗前主要证型为气阴两虚证、痰湿凝结证、脾胃虚寒证;治疗后主要证型为气阴两虚证、脾胃虚寒证、气血亏虚证。腹膜转移组治疗前肝胃不和证与痰湿凝结证肿瘤原发部位、分化程度、组织学、分期比较,差异并无统计学意义(P>0.05)。58例晚期GC患者PFS为(5.19±2.95)个月,其中腹膜转移组PFS为(4.22±2.77)个月,无腹膜转移组PFS为(7.02±2.40)个月,两组PFS比较,差异具有统计学意义(P<0.05)。将58例GC患者肝胃不和证、痰湿凝结证、气滞血瘀证归为实证,气阴两虚证、脾胃虚寒证、气血亏虚证归为虚证。实证与虚证PFS比较,差异无统计学意义(P>0.05)。结论:肝胃不和证和痰湿凝结证在晚期腹膜转移患者中多见,且生存期更短,免疫治疗可改善晚期GC患者中医证型的分布规律,加强对晚期GC患者中医证型的关注,并进行及早干预,有助于提高患者的生存期。

温阳活血汤联合艾灸治疗寒凝血瘀型痛经临床研究

目的:探讨温阳活血汤联合艾灸治疗寒凝血瘀型痛经临床疗效。方法:选取原发性寒凝血瘀型痛经患者60例,将其随机分为两组,其中对照组单纯接受艾灸腰阳关治疗,观察组则接受艾灸腰阳关联合温阳活血汤治疗。观察两组治疗后临床疗效,同时进行痛经症状评分,在干预前、干预3个月经周期后采用VAS评分量表评估两组疼痛程度。另取血清以酶联免疫法检测白细胞介素-8(IL-8)、超敏C反应蛋白(hs-CRP)和肿瘤坏死因子-α(TNF-α)水平及前列腺素E2(PGE2)、神经生长因子(NGF)和前列腺素F2α(PGF2α)水平。结果:对照组和观察组总有效率分别为76.67%和93.33%,观察组明显高于对照组(P<0.05)。治疗后3个月,两组的痛经相关中医症状评分均显著低于治疗前,且观察组的症状评分均显著降低(均P<0.05);两组的疼痛视觉模拟评分(VAS)评分和COX痛经症状量表(CMSS)评分均降低,且与对照组相比,观察组的VAS评分和CMSS评分更低(均P<0.05)。治疗后,两组IL-8、hs-CRP、TNF-α、PGE2、NGF和PGF2α水平均较治疗前显著降低,与对照组比较,观察组水平降低更明显(均P<0.05)。观察组和对照组的不良反应发生率分别为6.67%和10.00%(P>0.05)。结论:温阳活血汤联合艾灸可降低IL-8、hs-CRP、TNF-α、PGE2、NGF和PGF2α水平,改善患者痛经症状。

4℃保存全血制备的去白细胞混合浓缩血小板体外质量评价

目的 探讨全血4℃冷藏条件下保存制备去白细胞混合浓缩血小板的可行性,为成分制备提供理论依据。方法 将采集的400 mL ACD-B抗凝全血随机分两组,分别进行4℃和室温保存,在保存后6 h内分离制备出白膜层,于22℃静置过夜保存,次日将白膜层汇集制备去白细胞混合浓缩血小板,于制备后d1、d3、d5、d7取样,进行血细胞计数及相关参数检测,测定pH、葡萄糖、乳酸含量反映代谢情况,检测血栓弹力图、血小板聚集率及PAC-1、CD62P反映血小板体外功能及活化情况,比较两组血小板差异。结果 随着保存时间的延长,两组去白细胞混合浓缩血小板计数逐渐下降,血小板分布宽度(PDW)、平均血小板体积(MPV)、大型血小板比例(P-LCR)均逐渐上升,差异无统计学意义(P>0.05)。两组去白细胞混合浓缩血小板pH、葡萄糖含量均逐渐降低,乳酸含量逐渐增高,无显著性差异(P>0.05)。血栓弹力图结果显示,反应血小板功能的MA值保存期内无显著性变化,且两组间无显著性差异(P>0.05)。血小板聚集率随着保存时间的延长而逐渐降低,两组无统计学差异。血小板PAC-1及CD62P表达率随着保存时间的延长而逐渐升高,两组无统计学差异(P>0.05)。结论 全血4℃冷藏保存与室温保存6 h内制备的去白细胞混合浓缩血小板在体外计数、功能、活化方面无统计学差异,4℃冷藏6 h内的全血可考虑作为制备去白细胞混合浓缩血小板的起始血液。

壮医针灸治疗寒凝血瘀型原发性痛经疗效观察

目的:观察壮医针灸治疗寒凝血瘀型原发性痛经的临床疗效。方法:选取2020年6月-2021年6月广西中医药大学第一附属医院壮医针灸科60例原发性痛经患者,按照随机数字分组法分为观察组和对照组,每组各30例。对照组采用布洛芬片口服治疗;观察组采用壮医针灸治疗。连续治疗3个月经周期。分别在治疗1月后、治疗3月后及疗程后1个月比较两组患者VAS视觉模拟评分及中医证候积分变化,观察治疗3个月经周期后,两组患者治疗的临床疗效和不良反应发生率。结果:经治疗后,两组患者各时间点VAS评分及中医证候积分与治疗前比较,均得到明显改善(P<0.05)。组间比较,观察组中医证候积分改善程度均优于对照组,且起效快,疗效更稳定(P<0.05);观察组临床疗效优于对照组,不良反应发生率也低于对照组,差异均有统计学意义(P<0.05)。结论:壮医针灸治疗寒凝血瘀型原发性痛经起效快,能明显减轻疼痛程度,临床疗效显著、稳定,且无痛、操作简便,副作用少,值得临床推广应用。

少腹逐瘀汤治疗寒凝血瘀型原发性痛经有效性和安全性的meta分析

目的对少腹逐瘀汤治疗寒凝血瘀型原发性痛经(PD)的有效性和安全性进行系统评价。方法检索中文和外文数据库关于少腹逐瘀汤治疗寒凝血瘀型PD的随机对照试验。数据提取和质量评估分别由2名独立评估员进行,有效数据则使用RevMan 5.3.3软件进行meta分析。结果共纳入8个临床对照试验研究,收集病例797例,meta分析研究结果表明,对寒凝血瘀型PD患者,使用少腹逐瘀汤治疗痛经在总有效率、中医症候评分、疼痛视觉模拟评分、前列腺素E_(2)(PGE_(2))方面相较于单纯常规治疗差异有统计学意义(P<0.05)。结论寒凝血瘀型PD患者服用少腹逐瘀汤有较好的疗效,安全性较高,但仍存在一定的局限性,需要更多大样本、高质量、多中心、随机、双盲对照试验加以验证,提供更高级别的循证医学证据支持。

经皮电刺激配合艾灸八髎穴治疗寒凝血瘀型原发性痛经的临床效果

目的探讨经皮电刺激配合艾灸八髎穴治疗寒凝血瘀型原发性痛经的效果。方法选取2023年1月至6月江西省妇幼保健院收治的200例寒凝血瘀型原发性痛经患者作为研究对象,采用随机数字表法将其分为对照组与治疗组,每组100例。对照组采用布洛芬缓释胶囊治疗,治疗组采用经皮电刺激联合艾灸八髎穴治疗,比较两组患者的疗效、中医证候积分、疼痛评分、不良反应发生率。结果治疗组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。治疗组治疗3个月时的中医证候积分、视觉模拟评分法(VAS)评分均低于对照组,差异有统计学意义(P<0.05)。两组患者不良反应总发生率比较,差异无统计学意义(P>0.05)。结论采用经皮电刺激联合艾灸八髎穴对寒凝血瘀型原发性痛经患者进行治疗,可改善痛经症状,且安全性高,值得临床广泛应用及推广。

Dexamethasone prevents vascular damage in earlystage non-freezing cold injury of the sciatic nerve

Non-freezing cold injury is a prevalent cause of peripheral nerve damage, but its pathogenic mechanism is poorly understood, and treatment remains inadequate. Glucocorticoids have anti-inflammatory and lipid peroxidation-inhibiting properties. We therefore examined whether dexamethasone, a synthetic glucocorticoid compound, would alleviate early-stage non-freezing cold injury of the sciatic nerve. We established Wistar rat models of non-freezing cold injury by exposing the left sciatic nerve to cold（3–5°C） for 2 hours, then administered dexamethasone（3 mg/kg intraperitoneally） to half of the models. One day after injury, the concentration of Evans blue tracer in the injured sciatic nerve of rats that received dexamethasone was notably lower than that in the injured sciatic nerve of rats that did not receive dexamethasone; neither Evans blue dye nor capillary stenosis was observed in the endoneurium, but myelinated nerve fibers were markedly degenerated in the injured sciatic nerve of animals that received dexamethasone. After dexamethasone administration, however, endoneurial vasculopathy was markedly improved, although damage to the myelinated nerve fiber was not alleviated. These findings suggest that dexamethasone protects the blood-nerve barrier, but its benefit in non-freezing cold injury is limited to the vascular system.

雷火灸结合三伏贴治疗寒凝血瘀型冠心病心绞痛的临床效果

目的 分析雷火灸结合三伏贴治疗寒凝血瘀型冠心病心绞痛的临床效果,以期为临床该病的治疗提供参考依据。方法 选取2021年6月至2022年8月河南中医药大学第一附属医院收治的92例寒凝血瘀型冠心病心绞痛患者,随机分为两组,对照组应用三伏贴和瓜蒌薤白白酒汤,观察组在对照组的基础上应用雷火灸结合三伏贴治疗。干预前,干预1周后采用中医证候评分、西雅图心绞痛量表进行评价。结果 治疗后,两组1周内心绞痛发作次数、每次发作持续时间比较差异有统计学意义(P<0.05);观察组胸痛胸闷、心悸、畏寒肢冷、气短乏力、倦怠懒言、失眠多梦评分低于对照组(P<0.05);观察组治疗后西雅图心绞痛量表各维度评分高于对照组(P<0.05)。结论 雷火灸结合三伏贴治疗有助于改善寒凝血瘀型冠心病心绞痛患者临床症状,促进患者恢复。