For making better use of nucleic acid resources of Gossypium hirsutum, a data-mining method was used to identify putative genes responsive to various abiotic stresses in G. hirsutum. Based on the compiled database inc...For making better use of nucleic acid resources of Gossypium hirsutum, a data-mining method was used to identify putative genes responsive to various abiotic stresses in G. hirsutum. Based on the compiled database including genes involved in abiotic stress response in Arabidopsis thaliana and the comprehensive analysis tool of GENEVESTIGATOR v3, 826 genes up-regulated or down-regulated significantly in roots or leaves during salt or cold treatment in Arabidopsis were identified. As compared to these 826 Arabidopsis genes annotated, 38 homologous expressed sequence tags （ESTs） from G. hirsutum were selected randomly and their expression patterns were studied using a quantitative real-time reverse transcription-polymerase chain reaction method. Among these 38 ESTs, about 55% of the genes （21 of 38） were different in response to ABA between cotton and Arabidopsis, whereas 70% of genes had similar responses to cold and salt treatments, and some of them which had not been characterized in Arabidopsis are now being investigated in gene function studies. According to these results, this approach of analyzing ESTs appears effective in large-scale identification of cotton genes involved in abiotic stress and might be adopted to determine gene functions in various biologic processes in cotton.展开更多
为了在数据密集型工作流下有效降低缓存碎片整理开销并提高缓存命中率,提出一种持久性分布式文件系统客户端缓存DFS-Cache(Distributed File System Cache)。DFS-Cache基于非易失性内存(NVM)设计实现,能够保证数据的持久性和崩溃一致性...为了在数据密集型工作流下有效降低缓存碎片整理开销并提高缓存命中率,提出一种持久性分布式文件系统客户端缓存DFS-Cache(Distributed File System Cache)。DFS-Cache基于非易失性内存(NVM)设计实现,能够保证数据的持久性和崩溃一致性,并大幅减少冷启动时间。DFS-Cache包括基于虚拟内存重映射的缓存碎片整理机制和基于生存时间(TTL)的缓存空间管理策略。前者基于NVM可被内存控制器直接寻址的特性,动态修改虚拟地址和物理地址之间的映射关系,实现零拷贝的内存碎片整理;后者是一种冷热分离的分组管理策略,借助重映射的缓存碎片整理机制,提升缓存空间的管理效率。实验采用真实的Intel傲腾持久性内存设备,对比商用的分布式文件系统MooseFS和GlusterFS,采用Fio和Filebench等标准测试程序,DFS-Cache最高能提升5.73倍和1.89倍的系统吞吐量。展开更多
目的:基于数据挖掘探索针刺治疗风寒湿型冻结肩的有效经络及配穴规律。方法:通过Note-Express检索整合CNKI、Wanfang、VIP、Pub-Med、Science Direct、JAMA-Network、Web of Science等中英文数据库中近十年有关针刺治疗风寒湿型冻结肩的...目的:基于数据挖掘探索针刺治疗风寒湿型冻结肩的有效经络及配穴规律。方法:通过Note-Express检索整合CNKI、Wanfang、VIP、Pub-Med、Science Direct、JAMA-Network、Web of Science等中英文数据库中近十年有关针刺治疗风寒湿型冻结肩的RCT试验,对符合纳入标准的文献经络及配穴建立数据库,构建复杂网络,进行聚类分析及关联分析。结果:共纳入38篇文献,提取出57条针刺治疗风寒湿型冻结肩处方,涉及有效正经10条、腧穴33个。复杂网络得出肩髃、肩贞、肩髎各自所归的手阳明大肠经、手太阳小肠经和手少阳三焦经的度值位居前三;聚类分析得到3个高频腧穴聚类群及3个经络聚类群;关联分析得到肩髃、肩髎、肩贞、风池、阳陵泉为治疗风寒湿型冻结肩的核心组方。结论:针刺治疗风寒湿型冻结肩选穴以肩关节周围为主,远端配穴为辅,首选手三阳经经穴有法可循、有据可依。展开更多
基金Supports from Special Fund for Agro-Scientific Research in the Public Interest in China (3-19) the National Transgenic Plants Project of China(2008ZX08005-004) are kindly appreciated
文摘For making better use of nucleic acid resources of Gossypium hirsutum, a data-mining method was used to identify putative genes responsive to various abiotic stresses in G. hirsutum. Based on the compiled database including genes involved in abiotic stress response in Arabidopsis thaliana and the comprehensive analysis tool of GENEVESTIGATOR v3, 826 genes up-regulated or down-regulated significantly in roots or leaves during salt or cold treatment in Arabidopsis were identified. As compared to these 826 Arabidopsis genes annotated, 38 homologous expressed sequence tags （ESTs） from G. hirsutum were selected randomly and their expression patterns were studied using a quantitative real-time reverse transcription-polymerase chain reaction method. Among these 38 ESTs, about 55% of the genes （21 of 38） were different in response to ABA between cotton and Arabidopsis, whereas 70% of genes had similar responses to cold and salt treatments, and some of them which had not been characterized in Arabidopsis are now being investigated in gene function studies. According to these results, this approach of analyzing ESTs appears effective in large-scale identification of cotton genes involved in abiotic stress and might be adopted to determine gene functions in various biologic processes in cotton.
文摘为了在数据密集型工作流下有效降低缓存碎片整理开销并提高缓存命中率,提出一种持久性分布式文件系统客户端缓存DFS-Cache(Distributed File System Cache)。DFS-Cache基于非易失性内存(NVM)设计实现,能够保证数据的持久性和崩溃一致性,并大幅减少冷启动时间。DFS-Cache包括基于虚拟内存重映射的缓存碎片整理机制和基于生存时间(TTL)的缓存空间管理策略。前者基于NVM可被内存控制器直接寻址的特性,动态修改虚拟地址和物理地址之间的映射关系,实现零拷贝的内存碎片整理;后者是一种冷热分离的分组管理策略,借助重映射的缓存碎片整理机制,提升缓存空间的管理效率。实验采用真实的Intel傲腾持久性内存设备,对比商用的分布式文件系统MooseFS和GlusterFS,采用Fio和Filebench等标准测试程序,DFS-Cache最高能提升5.73倍和1.89倍的系统吞吐量。
文摘目的:基于数据挖掘探索针刺治疗风寒湿型冻结肩的有效经络及配穴规律。方法:通过Note-Express检索整合CNKI、Wanfang、VIP、Pub-Med、Science Direct、JAMA-Network、Web of Science等中英文数据库中近十年有关针刺治疗风寒湿型冻结肩的RCT试验,对符合纳入标准的文献经络及配穴建立数据库,构建复杂网络,进行聚类分析及关联分析。结果:共纳入38篇文献,提取出57条针刺治疗风寒湿型冻结肩处方,涉及有效正经10条、腧穴33个。复杂网络得出肩髃、肩贞、肩髎各自所归的手阳明大肠经、手太阳小肠经和手少阳三焦经的度值位居前三;聚类分析得到3个高频腧穴聚类群及3个经络聚类群;关联分析得到肩髃、肩髎、肩贞、风池、阳陵泉为治疗风寒湿型冻结肩的核心组方。结论:针刺治疗风寒湿型冻结肩选穴以肩关节周围为主,远端配穴为辅,首选手三阳经经穴有法可循、有据可依。
