Objective To study the characteristics and problems of the development of cold chain logistics in pharmaceutical industry,and to provide a reference for the development of China’s drug logistics.Methods Literature an...Objective To study the characteristics and problems of the development of cold chain logistics in pharmaceutical industry,and to provide a reference for the development of China’s drug logistics.Methods Literature analysis was used to investigate the development of drug cold chain logistics,relevant laws and regulations,and policies in China.Then,the problems of industry development were summarized and some suggestions were put forward by referring to the mature concept of international drug cold chain logistics system.Results and Conclusion China’s cold chain logistics in pharmaceutical field is growing,but it is still in the development stage.By increasing government support,scientific research investment and professional training,the comprehensive strength of drug cold chain logistics can be improved to a certain extent,and the safety of drugs can be guaranteed for the people.展开更多
Objective: To determine the hot/cold of South American A?aí(Euterpe oleracea Mart.). Materials and Methods: 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to compare the in...Objective: To determine the hot/cold of South American A?aí(Euterpe oleracea Mart.). Materials and Methods: 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to compare the influence of A?aí and phellodendron bark(Cortex Phellodendri Chinensis) on the growth and proliferation of HepG2 and Hep3 B cell strains. The morphological changes of the two cell strains treated with A?aí and C. Phellodendri were observed using an inverted phase contrast microscope. Trypan blue dye exclusion assay was used to compare and analyze the toxicity effects of A?aí. The content of nicotinamide adenine dinucleotide(NADH) and the ratio of NADH/NAD+ of the treated cells were detected using a spectrophotometer to determine the influence of A?aí to the energy metabolism of the two cell strains. Results: Within the range of measured concentrations, both A?aí and C. Phellodendri displayed separately the stronger inhibitory effects on cell growth, proliferation, and energy metabolism of the two cell strains. By MTT assay, A?aí showed significant(P < 0.05) or highly significant(P < 0.01) inhibitory effects on cell proliferation within the concentration range of 0.25–40 μg/mL, which was similar to the effects of C. Phellodendri. From the results of microscopic examination, the morphological of lower cell density, attenuated granularity, and more outstretched cells in irregular polygonal shape could be observed in the cells treated with A?aí, which was also similar to that of C. Phellodendri. In trypan blue staining assay, all concentrations of A?aí showed negligible toxicity effects, exactly as that of C. Phellodendri. A?aí showed a highly significant effect of decreasing the content of intracellular NADH as well as the ratio of NADH/NAD+(P < 0.05 and P < 0.01, respectively). Conclusion: The effect of A?aí on HepG2 and Hep3 B strains just as C. Phellodendri can reduce energy metabolism and inhibit the growth and proliferation. All performance characteristics of two cell strains treated with A?aí belong to that characteristic of cold property drug. From the study results, we can deduce that the drug property of A?aí is cold.展开更多
文摘Objective To study the characteristics and problems of the development of cold chain logistics in pharmaceutical industry,and to provide a reference for the development of China’s drug logistics.Methods Literature analysis was used to investigate the development of drug cold chain logistics,relevant laws and regulations,and policies in China.Then,the problems of industry development were summarized and some suggestions were put forward by referring to the mature concept of international drug cold chain logistics system.Results and Conclusion China’s cold chain logistics in pharmaceutical field is growing,but it is still in the development stage.By increasing government support,scientific research investment and professional training,the comprehensive strength of drug cold chain logistics can be improved to a certain extent,and the safety of drugs can be guaranteed for the people.
基金supported by grants from the Science and Technology Plan Project of Guangdong Province(Grant No.2014A070713031,2015A020209134)Science and Technology Plan Project of Guangzhou(Grant No.201604020171)
文摘Objective: To determine the hot/cold of South American A?aí(Euterpe oleracea Mart.). Materials and Methods: 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to compare the influence of A?aí and phellodendron bark(Cortex Phellodendri Chinensis) on the growth and proliferation of HepG2 and Hep3 B cell strains. The morphological changes of the two cell strains treated with A?aí and C. Phellodendri were observed using an inverted phase contrast microscope. Trypan blue dye exclusion assay was used to compare and analyze the toxicity effects of A?aí. The content of nicotinamide adenine dinucleotide(NADH) and the ratio of NADH/NAD+ of the treated cells were detected using a spectrophotometer to determine the influence of A?aí to the energy metabolism of the two cell strains. Results: Within the range of measured concentrations, both A?aí and C. Phellodendri displayed separately the stronger inhibitory effects on cell growth, proliferation, and energy metabolism of the two cell strains. By MTT assay, A?aí showed significant(P < 0.05) or highly significant(P < 0.01) inhibitory effects on cell proliferation within the concentration range of 0.25–40 μg/mL, which was similar to the effects of C. Phellodendri. From the results of microscopic examination, the morphological of lower cell density, attenuated granularity, and more outstretched cells in irregular polygonal shape could be observed in the cells treated with A?aí, which was also similar to that of C. Phellodendri. In trypan blue staining assay, all concentrations of A?aí showed negligible toxicity effects, exactly as that of C. Phellodendri. A?aí showed a highly significant effect of decreasing the content of intracellular NADH as well as the ratio of NADH/NAD+(P < 0.05 and P < 0.01, respectively). Conclusion: The effect of A?aí on HepG2 and Hep3 B strains just as C. Phellodendri can reduce energy metabolism and inhibit the growth and proliferation. All performance characteristics of two cell strains treated with A?aí belong to that characteristic of cold property drug. From the study results, we can deduce that the drug property of A?aí is cold.
