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Semi-Automated Enzymatic Determination of Ethanol in Beverages: Collaborative Study for RIDA®CUBE Ethanol
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作者 Markus Lacorn Thomas Hektor 《Food and Nutrition Sciences》 CAS 2023年第2期90-100,共11页
Easy and quick methods to quantify ethanol reliably in beverages are always important. In 2022, the Enzytec<sup>TM</sup> Liquid Ethanol test kit was approved as AOAC Official Method<sup>SM</sup>... Easy and quick methods to quantify ethanol reliably in beverages are always important. In 2022, the Enzytec<sup>TM</sup> Liquid Ethanol test kit was approved as AOAC Official Method<sup>SM</sup> 2017.07 Final Action after a collaborative study was conducted with different beverages such as kombucha, juices, and beer. During set-up of this collaborative test, small sized companies asked to include the RIDA<sup>&reg;</sup>CUBE Ethanol/RIDA<sup>&reg;</sup>CUBE SCAN device since it is easy to use, suitable for a few samples only and contains the identical reagents as the Enzytec<sup>TM</sup> Liquid system. It is applicable to quantify ethanol in diluted kombucha, fruit juices, and alcohol-free beer samples around 0.5% alcohol-by-volume within 12 min. The overall relative reproducibility standard deviation across a wide concentration range for kombucha, was calculated to be 6.29%. Analysis of juices and beer showed an overall higher variation with an estimated overall RSD(R) value by regression of 14.4%. The data obtained by this collaborative study show that the RIDA<sup>&reg;</sup>CUBE Ethanol in combination with the RIDA<sup>&reg;</sup>CUBE SCAN device is suitable to quantify ethanol from matrices representing important alcohol-free liquid food categories. 展开更多
关键词 ETHANOL Enzymatic Analysis Beverages AUTOMATION REPRODUCIBILITY collaborative Test
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