Study on Enzymatic Hydrolysis of Gadus morrhua Skin Collagen and Molecular Weight Distribution of Hydrolysates

Process parameters on enzymatic hydrolysis and molecular weight （MW） distribution of collagen hydrolysates from Gadus morrhua skin were investigated. The optimal process parameters were obtained by the single-factor and orthogonal experiments. The molecular weight distribution of hydrolysates was determined using both Sephadex G25 partition and high speed liquid chromatography electricity spray mass spectrum （HPLC-ESI-MS）. Collagen hydrolysates were first gained by an alkaline protease ＂alcalase＂ for 3 h at temperature （50~C）, pH （10.0）, substrate concentration （75 g L-~）, and E/S （3%）. The molecular weight distribution of collagen hydrolysates ranged from 300 to 1 500 Da, and most of peptides were under 1 200 Da. Sephadex G25 partition and HPLC-ESI-MS should be successfully employed to determine the molecular weight distribution of collagen hydrolysates.

鲮鱼鱼皮胶原蛋白酶解物分子量分布与抗氧化性的研究(英文)

[目的]为了研究鲮鱼鱼皮胶原蛋白水解物的分子量的分布及抗氧化活性。[方法]分子量分布采用分子排阻色谱法和基质辅助激光解吸附飞行时间质谱（MALDI-TOF-MS），鱼皮用碱性蛋白酶2709处理。[结果]最佳水解条件为：pH 10.0、反应温度55℃、底物浓度为80 g/L、酶和底物比4%，水解时间3 h。两种方法分析水解产物的相对分子质量分布范围为400-1800 Da,大多数胶原蛋白短肽的分子量在1400 Da以下。[结论]结果表明,鱼皮水解物是一种潜在的抗氧化物。