期刊文献+
共找到1,173篇文章
< 1 2 59 >
每页显示 20 50 100
An anti-aging skin strategy:promoting repair and regeneration in UV-induced photoaging micro-environment using growth factors-rich platelet lysates composite Self-protection Collagen Hydrogel
1
作者 Jian-Peng Zhang Zi-Duo Huang +5 位作者 Bing-Qing Wang Yi-Xin Chen Qing-Qing Zhou Hui-Qin Li Xin-Sheng Peng Yan-Fang Zhou 《Biomedical Engineering Communications》 2023年第2期7-17,共11页
Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chroni... Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chronic oxidative damage and inactivation of bio-macromolecule-based regeneration in oxidative photoaging micro-environment.In this study,we designed a“seed and soil”strategy to pursue a safer and more efficient way to prevent and treat photoaging by simultaneously changing UV-induced ROS-rich micro-environment into a proregenerative one(the“soil”)and providing growth factor-rich platelet lysates(PL,the“seed”)using PL-impregnated,collagen-reinforce hydrogel(PL/Col).SD rats were used to establish photoaging model by 8 weeks of UV irradiation.The effectiveness of different treatments was evaluated by making pathological sections and detecting photoaging-related indicators.Rats treated with PL/Col demonstrated a significant acceleration in skin healing and enhancement in the quality of trauma repairing.After treated with PL/Col,the rats showed smooth yellowish appearance,integral structure of skin collagen fiber and epidermis,a decrease in inflammation and a reshaped active micro-environment with reduced levels of SOD enzyme activity,GSH enzyme activity and MDA toxic products.Treatment of PL/Col in skin photoaging has shown potential anti-oxidation and anti-aging effects and is worthy of further study in related field. 展开更多
关键词 PHOTOAGING platelet derivatives oxidative stress growth factors collagen trauma repair
下载PDF
Design of Novel Wound Dressing Composed of Collagen and Hyaluronic Acid Containing Epidermal Growth Factor
2
作者 Yoshimitsu Kuroyanagi Ryusuke Suzuki Misato Kuroyanagi 《Open Journal of Regenerative Medicine》 2023年第2期49-63,共15页
This research aims to develop a wound dressing composed of collagen (Col) and hyaluronic acid (HA) containing epidermal growth factor (EGF). First important issue is to contain EGF in the wound dressing in a stable st... This research aims to develop a wound dressing composed of collagen (Col) and hyaluronic acid (HA) containing epidermal growth factor (EGF). First important issue is to contain EGF in the wound dressing in a stable state. The sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of Col. Both sides of sponge were treated with ultraviolet (UV) irradiation to introduce intermolecular cross links between collagen molecules. This sponge was named Sponge-Col. Another sheet-shaped sponge was manufactured by freeze-vacuum drying an aqueous solution of HA containing EGF. This sponge was named Sponge-HA/EGF. The wound dressing was manufactured by laminating Sponge-Col on the top, Sponge-HA/EGF in the middle, and Sponge-Col on the bottom to create a sandwich structure. This method can prevent the reducing of EGF activity due to UV irradiation for intermolecular cross-linking. Second important issue is to enable gradual release of EGF from the wound dressing. The elution behavior of this wound dressing was investigated by measuring the weight change after immersion in water for a predetermined time. This wound dressing showed initially fast elution and subsequent very slow elution properties. The upper layer and lower layer Sponge-Col enabled gradual release of the middle layer Sponge-HA/EGF. This result suggests that EGF contained in the wound dressing is gradually released together with HA from the wound dressing. Third important issue is to provide moist wound-healing environment. The upper layer and lower layer Sponge-Col can provide the wound dressing with high water absorption and long-term water retention properties. 展开更多
关键词 collagen Hyaluronic Acid Epidermal Growth factor Wound Dressing
下载PDF
Vascular endothelial growth factor A promotes platelet adhesion to collagen Ⅳ and causes early brain injury after subarachnoid hemorrhage 被引量:4
3
作者 Zun-Wei Liu Jun-Jie Zhao +1 位作者 Hong-Gang Pang Jin-Ning Song 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第10期1726-1733,共8页
The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to p... The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to produce a rat model of subarachnoid hemorrhage.Then,30 minutes later,vascular endothelial growth factor A antagonist anti-vascular endothelial growth factor receptor 2 antibody,10μg,was injected into the right ventricle.Immunohistochemistry and western blot assay were used to assess expression of vascular endothelial growth factor A,occludin and claudin-5.Immunohistochemical double labeling was conducted to examine co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen.TUNEL was used to detect apoptosis in the hippocampus.Neurological score was used to assess behavioral performance.After subarachnoid hemorrhage,the expression of vascular endothelial growth factor A increased in the hippocampus,while occludin and claudin-5 expression levels decreased.Co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells increased,whereas behavioral performance was markedly impaired.After treatment with anti-vascular endothelial growth factor receptor 2 antibody,occludin and claudin-5 expression recovered,while co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells decreased.Furthermore,behavioral performance improved notably.Our findings suggest that increased vascular endothelial growth factor A levels promote platelet adhesion and contribute to early brain injury after subarachnoid hemorrhage.This study was approved by the Biomedical Ethics Committee,Medical College of Xi’an Jiaotong University,China in December 2015. 展开更多
关键词 nerve REGENERATION VASCULAR ENDOTHELIAL GROWTH factor A VASCULAR ENDOTHELIAL GROWTH factor receptor 2 subarachnoid hemorrhage brain injuries platelet adhesion collagen blood-brain barrier neural REGENERATION
下载PDF
Effects of connective tissue growth factor and collagen type Ⅰ scleroderma
4
作者 Xiaoning Yan Jie Feng Bingjun Shi 《Journal of Nanjing Medical University》 2007年第3期175-179,共5页
Objective: To investigate the effects of connective tissue growth factor(CTGF) and collagen type I(COL-I) on the pathogenesis of scleroderma and explore the relationship between the level of COL-I and CTGF. Meth... Objective: To investigate the effects of connective tissue growth factor(CTGF) and collagen type I(COL-I) on the pathogenesis of scleroderma and explore the relationship between the level of COL-I and CTGF. Methods: 12 mice model of scleroderma was established by the injection of Bleomycin. The level of CTGF and COL-I were detected by immunohistochemical method. The relationship was analyzed between CTGF and COL-I level. As control group, 12 healthy mice were selected. Results: The levels of CTGF and COL-I in sclerotic models were higher than in normal controls (P 〈 0.05). It was found that there was a correlation between the level of CTGF and COL-I. Conclusion: CTGF and COL-I played an important role in the hardening process of the skin lesions of the mice model, which may be involved in the pathogenesis of scleroderma. 展开更多
关键词 connective tissue growth factor collagen type I SCLERODERMA
下载PDF
Effects of connective tissue growth factor antisense oligonucleotides on the proliferation and collagen synthesis of the cultured human keloid fibroblasts in vitro
5
作者 刘剑毅 李世荣 纪淑兴 《Journal of Medical Colleges of PLA(China)》 CAS 2004年第4期211-213,共3页
Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encap... Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblasts (HKFs) culture media. The intracellular distribution of CTGF ASODN was observed by fluorescence microscopy in the fixed HKFs. The proliferation of HKFs was measured by MTT test. The collagen synthesis of HKFs was measured by 3H-proline incorporation method. Results: Compared with control group, the CTGF ASODN can inhibit the proliferation and collagen synthesis of the HKFs (P<0.01). Conclusion: CTGF ASODN has anti-fibrotic effects on keloid in vitro, and CTGF play an important role in promoting the fibrosis of keloid. 展开更多
关键词 connective tissue growth factor antisense oligonucleotides KELOID FIBROBLAST PROLIFERATION collagen synthesis
下载PDF
Modified insulin-like growth factor 1 containing collagen-binding domain for nerve regeneration 被引量:1
6
作者 Jian-an Li Chang-fu Zhao +5 位作者 Shao-jun Li Jun Zhang Zhen-hua Li Qiao Zhang Xiao-yu Yang Chun-fang Zan 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第2期298-303,共6页
Insulin-like growth factor 1 (IGF-I) is a potential nutrient for nerve repair. However, it is impractical as a therapy because of its limited half- life, rapid clearance, and limited target specificity. To achieve t... Insulin-like growth factor 1 (IGF-I) is a potential nutrient for nerve repair. However, it is impractical as a therapy because of its limited half- life, rapid clearance, and limited target specificity. To achieve targeted and long-lasting treatment, we investigated the addition of a binding structure by fusing a collagen-binding domain to IGF- 1. After confirming its affinity for collagen, the biological activity of this construct was examined by measuring cell proliferation after transfection into PC12 and Schwann cells using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-di- phenyl-2-H-tetrazolium bromide assay. Immunofluorescence staining was conducted to detect neurofilament and microtubule-associated protein 2 expression, while real time-polymerase chain reaction was utilized to determine IGF-1 receptor and nerve growth/actor mRNA expression. Our results demonstrate a significant increase in collagen-binding activity of the recombinant protein compared with IGF-1. Moreover, the recombinant protein promoted proliferation of PC12 and Schwann cells, and increased the expression of neurofilament and microtubule-associated protein 2. Importantly, the recombinant protein also stimulated sustained expression of IGF-1 receptor and nerve growth factor mRNA for days. These results show that the recombinant protein achieved the goal of targeting and long-lasting treatment, and thus could become a clinically used factor for promoting nerve regeneration with a prolonged therapeutic effect. 展开更多
关键词 nerve regeneration insulin-like growth factor I collagen-binding domain fusion protein collagenASE targeted therapy neural regeneration
下载PDF
不同软组织替代物在比格犬尖牙唇侧区域移植后附着龈的厚度和组织学变化
7
作者 张红霞 栗兴超 +4 位作者 高玺鑫 张晓 梅双 马寒夕 张天 《中国组织工程研究》 CAS 北大核心 2024年第11期1660-1665,共6页
背景:上皮下结缔组织移植被认为是软组织增量术的金标准,然而,自体移植需要开辟第二术区,延长手术时间,增加患者的疼痛感,这也使得软组织替代物成为研究热点。目的:比较不同软组织替代物在比格犬尖牙唇侧区域移植后移植区附着龈厚度和... 背景:上皮下结缔组织移植被认为是软组织增量术的金标准,然而,自体移植需要开辟第二术区,延长手术时间,增加患者的疼痛感,这也使得软组织替代物成为研究热点。目的:比较不同软组织替代物在比格犬尖牙唇侧区域移植后移植区附着龈厚度和组织学变化。方法:在3只比格犬尖牙唇侧附着龈区域,分别用双层同种异体脱细胞真皮基质膜(allograft acellular dermal matrix,AADM)、牛源性脱细胞真皮基质(bovine acellular dermal matrix,BADM)联合浓缩生长因子膜以及BADM联合胶原蛋白海绵进行附着龈增厚。分别在术前和术后1,2,3,4个月测量附着龈厚度,术后4个月进行组织学观察。结果与结论:①术后第1-4个月,双层AADM组附着龈厚度及附着龈厚度增量均值高于其他2组(均P<0.05);②术后1个月3组附着龈厚度显著增加,而后呈下降趋势;术后3个月,BADM联合胶原蛋白海绵组与BADM联合浓缩生长因子组移植区附着龈厚度变化较术前差异无显著性意义(P>0.05);术后4个月,双层AADM组附着龈厚度均值较术前仍显著增加(P<0.05);第1-4个月双层AADM组附着龈厚度值最高,其次为BADM联合胶原蛋白海绵组,BADM联合浓缩生长因子组最低;③双层AADM组真皮基质与宿主牙龈组织良好的结合在一起,并可见新生纤维结缔组织和成纤维细胞长入基质内,另外两组移植物已经完全吸收,增量区域改建成与周围宿主组织一致的结构;④结果说明,AADM在牙龈增厚方面优于BADM联合浓缩生长因子膜和BADM联合胶原蛋白海绵。 展开更多
关键词 脱细胞真皮基质 浓缩生长因子 胶原蛋白海绵 牙龈增厚
下载PDF
蕲艾挥发油通过PI3K/Akt信号通路促进感染性皮肤创口愈合的机制研究
8
作者 吕丰 梁云靖 +2 位作者 宋伟 姚雨峰 洪宗国 《南京中医药大学学报》 CAS CSCD 北大核心 2024年第8期795-803,共9页
目的观察蕲艾(Artemisia argyi Levl.et Van.var.argyi cv.Qiai)挥发油对感染性皮肤创口愈合的作用并探索其机制。方法将30只雄性昆明小鼠随机分为5组:空白对照组、膜剂基质组、阳性对照组(3 mL·kg^(-1)莫匹罗星软膏)、低剂量膜剂... 目的观察蕲艾(Artemisia argyi Levl.et Van.var.argyi cv.Qiai)挥发油对感染性皮肤创口愈合的作用并探索其机制。方法将30只雄性昆明小鼠随机分为5组:空白对照组、膜剂基质组、阳性对照组(3 mL·kg^(-1)莫匹罗星软膏)、低剂量膜剂组(每贴含7.3 mg蕲艾挥发油)和高剂量膜剂组(每贴含57.6 mg蕲艾挥发油)。小鼠麻醉后在背部剪一直径10 mm的全层皮肤圆形伤口,止血后在创口处涂抹100μL金黄色葡萄球菌菌液(1.5×10^(8) CFU·mL^(-1)),各组采取前述方式处理伤口,每日换药并观察皮肤创面愈合情况。术后12 d处死小鼠取创面皮肤做HE和Masson染色,观察病理学特征;利用qPCR和Western blot检测创口皮肤组织愈合相关因子Col1a1、Col3a1、Fn1、VEGFA及炎症因子IL-1β、IL-6、TNF-αmRNA和PI3K/Akt信号通路蛋白的表达。在小鼠胚胎成纤维细胞NIH-3T3利用PI3K抑制剂PI-103探究蕲艾挥发油对PI3K/Akt信号通路活性的潜在影响。结果动物实验表明,与空白对照组相比,低剂量和高剂量膜剂组的相对创口面积显著降低;创口组织的炎性细胞浸润减少,胶原纤维表达增加;qPCR显示,与空白对照组相比,低剂量和高剂量膜剂组的VEGFA、Col3a1和Col1a1 mRNA的表达显著上升,炎症因子IL-1β和TNF-αmRNA表达降低;Western blot结果表明,与空白对照组相比,低剂量和高剂量给药组的p-PI3K/PI3K及p-Akt/Akt比值均显著上升并呈现剂量依赖性。细胞实验结果也证实,蕲艾挥发油对NIH-3T3细胞的PI3K/Akt信号通路存在调控作用。结论蕲艾挥发油可促进创口愈合,其机制可能与激活PI3K/Akt信号通路,抑制炎症因子表达及促进胶原表达有关。 展开更多
关键词 挥发油 皮肤创口 PI3K/AKT信号通路 炎症因子 胶原蛋白
下载PDF
miRNA-424-5p靶向VEGFA对胎衣不下奶牛胎盘胶原蛋白降解的影响
9
作者 刘炳琦 罗春海 +4 位作者 姚伟佳 王薇 刘佳金 李丹阳 付世新 《中国农业科学》 CAS CSCD 北大核心 2024年第15期3083-3092,共10页
【目的】通过探究miRNA-424-5p对奶牛母体胎盘组织胶原蛋白降解的影响,进而明确miRNA-424-5p对奶牛胎衣不下(retained fetal membranes,RFM)发生的调控作用及机制。【方法】检测胎衣正常排出与胎衣不下奶牛母体胎盘组织miRNA-424-5p表... 【目的】通过探究miRNA-424-5p对奶牛母体胎盘组织胶原蛋白降解的影响,进而明确miRNA-424-5p对奶牛胎衣不下(retained fetal membranes,RFM)发生的调控作用及机制。【方法】检测胎衣正常排出与胎衣不下奶牛母体胎盘组织miRNA-424-5p表达水平,采用qRT-PCR和Western blot方法进行胎衣正常排出与胎衣不下奶牛的母体胎盘组织VEGFA、MMP-2、MMP-9、COL-IV的表达水平检测。应用生物信息学分析对miRNA-424-5p进行靶基因预测,并通过双荧光素酶试验验证miRNA-424-5p与VEGFA的靶向关系。在奶牛子宫内膜上皮细胞中转染miRNA-424-5p mimics和miRNA-424-5p inhibitor进行过表达和沉默miRNA-424-5p。采用免疫荧光技术观察VEGFA在奶牛子宫内膜上皮细胞的表达变化,qRT-PCR和Western blot检测VEGFA、MMP-2、MMP-9、COL-IV的mRNA与蛋白表达水平的变化。【结果】与健康组相比,RFM组母体胎盘组织中的miRNA-424-5p mRNA表达水平显著升高(P<0.01),VEGFA、MMP-2、MMP-9 mRNA与蛋白表达水平极显著降低(P<0.01),COL-IV mRNA与蛋白表达水平极显著升高(P<0.01)。miRNA-424-5p的潜在靶基因共有152个,并经双荧光素酶试验结果证实VEGFA是miRNA-424-5p的靶基因。过表达和沉默miRNA-424-5p后,免疫荧光结果显示,与对照组相比miRNA-424-5p mimics组VEGFA的表达较低,而miRNA-424-5p inhibitor组VEGFA表达较高;qRT-PCR和Western blot结果显示,过表达miRNA-424-5p,靶基因VEGFA mRNA与蛋白表达水平极显著降低,基质金属蛋白酶MMP-2、MMP-9 mRNA与蛋白表达水平极显著降低(P<0.01),胶原蛋白COL-IV mRNA与蛋白表达水平极显著升高(P<0.01),而沉默miRNA-424-5p时VEGFA、MMP-2、MMP-9 mRNA与蛋白表达显著升高(P<0.01),COL-IV mRNA与蛋白表达水平极显著降低。【结论】miRNA-424-5p可靶向VEGFA调控MMPs表达、胶原蛋白的分解从而引起细胞外胶原降解障碍,是引起胎衣不下发生的重要因素。 展开更多
关键词 奶牛 胎衣不下 miRNA-424-5p 血管内皮生长因子A 胶原蛋白降解
下载PDF
成纤维细胞生长因子受体1 抑制剂对胶原诱导关节炎模型大鼠骨破坏的影响
10
作者 韩海慧 孟晓辉 +3 位作者 徐博 冉磊 施杞 肖涟波 《中国组织工程研究》 CAS 北大核心 2025年第5期968-977,共10页
背景:课题组前期的研究表明靶向成纤维细胞生长因子受体1(fibroblast growth factor receptor 1,FGFR1)可能是治疗类风湿性关节炎的有效靶点。目的:探讨FGFR1抑制剂(PD173074)对胶原诱导关节炎模型大鼠骨破坏的影响。方法:将25只雌性SD... 背景:课题组前期的研究表明靶向成纤维细胞生长因子受体1(fibroblast growth factor receptor 1,FGFR1)可能是治疗类风湿性关节炎的有效靶点。目的:探讨FGFR1抑制剂(PD173074)对胶原诱导关节炎模型大鼠骨破坏的影响。方法:将25只雌性SD大鼠随机分为5组,正常对照组、模型组、甲氨蝶呤组、PD173074低剂量组、PD173074高剂量组。除正常对照组外,其余各组大鼠建立Ⅱ型胶原诱导关节炎模型。造模成功后正常组及模型组大鼠腹腔注射无菌PBS,甲氨蝶呤组药物注射剂量为1.04 mg/kg,PD173074低剂量组和高剂量组药物注射剂量分别为5,20 mg/kg,1次/周。给药4周后取材,观察大鼠临床症状以及关节肿胀情况,踝关节Micro-CT三维重建及分析,观察踝关节病理变化,检测关节周围血管生成情况及核因子κB受体活化因子配体的表达,检测关节滑膜中p-FGFR1、血管内皮生长因子A、抗酒石酸酸性磷酸酶的表达,观察肝、脾、肾病理变化并计算肝、脾、肾指数。结果与结论:①PD173074能够减轻模型大鼠踝关节临床症状及关节肿胀,延缓骨质丢失,改善骨结构,减轻关节滑膜侵袭以及软骨骨侵蚀,降低关节周围破骨细胞数量,抑制关节滑膜组织中的血管生成,降低核因子κB受体活化因子配体的表达,抑制FGFR1磷酸化蛋白、抗酒石酸酸性磷酸酶和血管内皮生长因子A的蛋白表达。②大鼠肝、脾、肾病理观察表明经过PD173074治疗后无明显的毒副作用。③研究证明了FGFR1抑制剂能够延缓Ⅱ型胶原诱导关节炎模型大鼠关节炎症及骨破坏的进展,并抑制血管的生成。初步验证了PD173074在Ⅱ型胶原诱导关节炎模型中的治疗作用,其可能是通过抑制FGFR1磷酸化发挥作用,为寻找类风湿性关节炎新的治疗靶点提供了方向。 展开更多
关键词 类风湿关节炎 PD173074 成纤维细胞生长因子受体1 胶原诱导型关节炎 动物模型 骨破坏 血管生成
下载PDF
屏障材料自体浓缩生长因子膜和骨胶原在后牙区牙槽嵴保存术中的应用:一项1年随访的前瞻性队列研究
11
作者 朱湛枫 杨婷婷 +4 位作者 陈沁怡 邱伟恩 黎詠珊 林意蓝 班宇 《华西口腔医学杂志》 CAS CSCD 北大核心 2024年第3期346-352,共7页
目的评价自体浓缩生长因子(CGF)膜和骨胶原作为屏障材料在后牙区牙槽嵴保存术(ARP)术后1年的骨组织保存效果。方法选取三壁及以上骨缺损需接受后牙区牙槽嵴保存术治疗的24例患者为研究对象,随机分配至CGF组(12例)和骨胶原组(Collagen组)... 目的评价自体浓缩生长因子(CGF)膜和骨胶原作为屏障材料在后牙区牙槽嵴保存术(ARP)术后1年的骨组织保存效果。方法选取三壁及以上骨缺损需接受后牙区牙槽嵴保存术治疗的24例患者为研究对象,随机分配至CGF组(12例)和骨胶原组(Collagen组)(12例)。2组患者均拔除无法保留后牙,拔牙窝内填充异种移植物骨替代物Bio‐Oss®至拔牙前牙槽嵴顶处,CGF组将制取的CGF膜覆盖于骨替代材料上缘并封闭创口,Collagen组采用Bio-Oss®Collagen覆盖并封闭创口。牙槽峭保存术后6个月植入种植体。采用锥形束CT测量分析术后即刻、6个月和1年的垂直牙槽嵴骨高度和水平牙槽嵴骨宽度变化,评估种植术中再植骨率和植体存留率。采用SPSS 28.0软件对数据进行统计学分析。结果24例患者均完成术后1年随访,无1例退出试验或失访,无1例出现术后感染、出血和种植体周病等。术后6个月CGF组的垂直牙槽嵴骨高度减少量低于Collagen组,差异有统计学意义(P<0.05);术后1年CGF组和Collagen组垂直牙槽嵴骨高度的减少量差异无统计学意义(P>0.05)。术后6个月、术后1年CGF组和Collagen组水平牙槽嵴骨宽度的减少量差异均无统计学意义(P>0.05)。2组种植术中再植骨率均为16.7%,植体存留率为100%。结论CGF膜和Bio-Oss®Collagen作为后牙区牙槽嵴保存术的屏障材料,均能有效减少拔牙后牙槽骨吸收,保存牙槽骨。 展开更多
关键词 牙槽嵴保存术 自体浓缩生长因子 骨胶原
下载PDF
FGF+Collagen联合百多邦治疗烧伤后残余创面对炎性因子表达及创面愈合的影响 被引量:13
12
作者 徐丽红 《河北医科大学学报》 CAS 2017年第10期1168-1171,共4页
目的探讨成纤维细胞生长因子(fibroblast growth factor,FGF)+胶原蛋白(Collagen)联合百多邦治疗烧伤后残余创面对炎性因子表达及创面愈合的影响。方法 80例患者随机分为对照组和观察组各40例。对照组给予百多邦治疗,观察组给予创必复(F... 目的探讨成纤维细胞生长因子(fibroblast growth factor,FGF)+胶原蛋白(Collagen)联合百多邦治疗烧伤后残余创面对炎性因子表达及创面愈合的影响。方法 80例患者随机分为对照组和观察组各40例。对照组给予百多邦治疗,观察组给予创必复(FGF+Collagen)生物蛋白海绵联合百多邦治疗。比较2组临床疗效、创面修复情况、炎症因子表达水平以及创面愈合质量。结果观察组临床疗效和总有效率明显优于或高于对照组(P<0.05)。观察组肉芽组织生长良好率、细菌培养阴性率、创面细菌清除率均明显高于对照组,而肉芽组织生长时间、创面愈合时间以及视觉模拟评分明显低于对照组(P<0.05)。观察组色素沉着、瘢痕增生、创面加深以及创面感染等发生率明显低于对照组(P<0.05)。治疗前,2组创面IL-1β和TNF-α表达水平差异均无统计学意义(P>0.05);治疗后14 d,2组创面IL-1β和TNF-α表达水平均较治疗前明显降低,但观察组较对照组下降更明显(P<0.05)。结论 FGF+Collagen联合百多邦治疗烧伤后残余创面疗效显著,能够有效促进创面愈合,减轻瘢痕增生,降低创面炎症因子表达,抑制细菌生长,从而减少或避免创面感染的发生,值得临床推广应用。 展开更多
关键词 烧伤 成纤维细胞生长因子 胶原蛋白 百多邦
下载PDF
内质网应激蛋白激酶RNA样ER激酶(PERK)通路对肝星状细胞激活及Ⅰ型胶原蛋白表达的影响
13
作者 黎凤炎 刘泽峰 +4 位作者 夏雨艳 王文娟 李琪 唐利瑕 张国 《临床肝胆病杂志》 CAS 北大核心 2024年第5期968-974,共7页
目的探讨内质网应激蛋白激酶RNA样ER激酶(PERK)/真核生物翻译起始因子2α(eIF2α)信号通路对肝星状细胞(HSC)活化的影响。方法收集11例肝穿刺病理提示S1~S4肝纤维化患者和9例肝血管瘤、肝腺瘤患者术后周围正常肝组织病理切片,进一步行... 目的探讨内质网应激蛋白激酶RNA样ER激酶(PERK)/真核生物翻译起始因子2α(eIF2α)信号通路对肝星状细胞(HSC)活化的影响。方法收集11例肝穿刺病理提示S1~S4肝纤维化患者和9例肝血管瘤、肝腺瘤患者术后周围正常肝组织病理切片,进一步行组织免疫组化检测PERK、eIF2α、C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白(CHOP)表达情况;使用不同浓度的内质网应激诱导剂毒胡萝卜素(0、125、250、500、1000 nmol/L)作用于人HSC-LX2,使用qRT-PCR检测PERK mRNA及Western Blot检测PERK、肌醇需要酶1(IRE1)、激活转录因子6(ATF6)、CHOP及α平滑肌肌动蛋白(α-SMA)表达水平。使用慢病毒转染构建PERK稳定过表达LX-2组及对照组,并通过qRT-PCR检测PERK、eIF2α、α-SMA mRNA,Western Blot检测PERK、p-eIF2α、α-SMA蛋白表达,免疫荧光检测胶Ⅰ型原蛋白(COL1A1)表达。符合正态分布的计量资料两组间比较采用成组t检验,多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验;不符合正态分布的计数资料,两组间比较采用Mann-Whitney U秩和检验。结果与正常肝组织相比,肝纤维化患者肝组织中PERK、eIF2α及CHOP表达升高,差异均有统计学意义(Z=−3.56、t=−5.75、Z=−3.52,P值均<0.001)。不同浓度毒胡萝卜素作用后,与溶媒组相比,内质网相关蛋白PERK、CHOP、IRE1、ATF6及α-SMA蛋白表达显著升高(P值均<0.05)。与对照空载慢病毒组相比,PERK稳定过表达组PERK mRNA、eIF2αmRNA、α-SMA mRNA表达及PERK、p-eIF2α、α-SMA蛋白表达明显升高(P值均<0.05)。细胞免疫荧光结果提示,PERK过表达组COL1A1表达升高(P<0.05)。结论PERK过表达可诱导LX-2细胞α-SMA、胶原蛋白COL1A1表达,提示PERK信号通路可能是HSC活化的重要机制之一。 展开更多
关键词 内质网应激 真核细胞起始因子2 肝星状细胞 胶原Ⅰ型
下载PDF
TGF-β1、MMP-3及Collagen Ⅲ在POP患者子宫主韧带组织中的表达及意义 被引量:6
14
作者 张凯 薛惠英 张雪芹 《分子诊断与治疗杂志》 2020年第1期39-43,共5页
目的探讨转化生长因子-β1(TGF-β1)、基质金属蛋白酶-3(MMP-3)及胶原蛋白Ⅲ型(CollagenⅢ)在盆腔器官脱垂(POP)患者子宫主韧带组织中的表达及意义。方法选取2017年1月至2018年5月治疗的POP患者45例(POP组),其中Ⅲ期25例,Ⅳ期20例,同时... 目的探讨转化生长因子-β1(TGF-β1)、基质金属蛋白酶-3(MMP-3)及胶原蛋白Ⅲ型(CollagenⅢ)在盆腔器官脱垂(POP)患者子宫主韧带组织中的表达及意义。方法选取2017年1月至2018年5月治疗的POP患者45例(POP组),其中Ⅲ期25例,Ⅳ期20例,同时选取无POP患者50例作为对照组,采用免疫组化染色分析子宫主韧带组织中TGF-β1、MMP-3及CollagenⅢ表达情况,Western blot检测各指标表达的灰度值。结果POP组TGF-β1和CollagenⅢ阳性表达率及平均灰度值均明显低于对照组(P<0.05),而MMP-3阳性表达率及平均灰度值明显高于对照组(P<0.05)。POP组绝经前后后患者TGF-β1、CollagenⅢ、MMP-3阳性表达率及平均灰度值差异具有统计学意义(P<0.05)。POP组Ⅲ期和Ⅳ期患者TGF-β1、CollagenⅢ、MMP-3阳性表达率及平均灰度值差异具有统计学意义(P<0.05)。POP患者TGF-β1、CollagenⅢ与MMP-3呈负相关(r=-0.563,-0.677,P<0.05),TGF-β1与CollagenⅢ呈正相关(r=0.441,P<0.05)。结论POP患者子宫主韧带组织中MMP-3呈高表达,而TGF-β1与CollagenⅢ呈低表达,与绝经状况及病情程度可能有一定关系,且3者间表达有相关性。 展开更多
关键词 转化生长因子Β1 基质金属蛋白酶3 胶原蛋白 Ⅲ型 盆腔器官脱垂
下载PDF
Sublytic C5b-9诱导肾小球系膜细胞产生的TSP-1和TGF-β1对其合成FN和collagenⅣ的影响 被引量:1
15
作者 邱文 车楠 +3 位作者 周建博 李妍 赵聃 王迎伟 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第11期1543-1549,共7页
目的:探讨亚溶解型(sublytic)C5b-9复合物刺激大鼠肾小球系膜细胞(glomerular mesangial cell,GMC)后,诱生的血小板反应蛋白-1(thrombospondin-1,TSP-1)与转化生长因子-β1(transforming growth factor-β1,TGF-β1)对其促进细胞外基质(... 目的:探讨亚溶解型(sublytic)C5b-9复合物刺激大鼠肾小球系膜细胞(glomerular mesangial cell,GMC)后,诱生的血小板反应蛋白-1(thrombospondin-1,TSP-1)与转化生长因子-β1(transforming growth factor-β1,TGF-β1)对其促进细胞外基质(ex-tracellular matrix,ECM),如纤维连接蛋白(fibronectin,FN)和Ⅳ型胶原蛋白(collagenⅣ)合成的影响。方法:体外培养大鼠GMC,进行不同分组处理并给予sublytic C5b-9刺激,然后分别检查受刺激的GMC合成TSP-1和TGF-β1因子水平,同时检测GMC分泌FN和collagenⅣ的水平。此外,应用人工合成的TSP-1封闭肽段(GGWSHW)和TGF-β1中和抗体处理培养的大鼠GMC,研究TSP-1和TGF-β1在sublytic C5b-9诱导的GMC分泌上述ECM中的作用及其相互关系。结果:培养的大鼠GMC在sublytic C5b-9刺激后18 h,其FN和collagenⅣ表达水平均明显升高。同时,TSP-1蛋白表达和TGF-β1分泌(包括活化的TGF-β1含量)也显著增多。用TSP-1封闭肽段(GGWSHW)处理大鼠GMC后亦能显著抑制由sublytic C5b-9诱导的TGF-β1活化,并减少FN、collagenⅣ的产生。同样用TGF-β1中和抗体处理GMC后也能明显抑制由sublytic C5b-9导致的FN、collagenⅣ的分泌。结论:体外用sublytic C5b-9刺激大鼠GMC,能诱导其ECM分泌与TSP-1和TGF-β1的合成及活化,而sublytic C5b-9促进GMC分泌ECM的机制可能与其诱导TSP-1合成及活化的TGF-β1存在一定的关系。 展开更多
关键词 sublytic C5B-9 肾小球系膜细胞 血小板反应蛋白-1 转化生长因子-β1 纤维连接蛋白 Ⅳ型胶原蛋白
下载PDF
TGF-β_1和CollagenⅢ在压力性尿失禁患者阴道壁组织中表达的研究 被引量:1
16
作者 赵红 李际春 《中国医刊》 CAS 2010年第2期39-42,共4页
目的探讨压力性尿失禁(SUI)患者盆底组织中转化生长因子-β_1(TGF-β_1)与CollagenⅢ与SUI的关系。方法收集SUI(伴或不伴POP)患者67例,并选取同期因子宫良性病变而非卵巢功能性肿瘤行手术治疗的非SUI和POP患者20例作为对照。术中取阴道... 目的探讨压力性尿失禁(SUI)患者盆底组织中转化生长因子-β_1(TGF-β_1)与CollagenⅢ与SUI的关系。方法收集SUI(伴或不伴POP)患者67例,并选取同期因子宫良性病变而非卵巢功能性肿瘤行手术治疗的非SUI和POP患者20例作为对照。术中取阴道前壁组织。免疫组化方法检测CollagenⅢ及TGF-β_1,的表达阳性率。结果①Collagen Ⅲ在SUI组、SUI+POP组及对照组患者表达阳性率分别为25.0%、22.5%、90.0%;TGF-β_1在SUI组、SUI+POP组及对照组患者表达阳性率分别为26.8%、22.6%、90.O%。SUI组及SUI+pOP组与对照组比较,CollagenⅢ和TGF-β_1的表达均低于对照组(P<0.01),而在SUI组与SUI+POP组比较,差异无统计学显著意义(P>0.05)。②TGF-β_1表达水平与Collagen Ⅲ表达水平呈正相关。结论 SUI患者盆底支持组织的弹性和强度减弱与组织中TGF-β_1减少和胶原蛋白含量下降有关。 展开更多
关键词 压力性 尿失禁 转化生长因子-Β1 胶原
下载PDF
Do tensile and shear forces exerted on cells influence mechanotransduction through stored energy considerations?
17
作者 FREDERICK H.SILVER TANMAY DESHMUKH 《BIOCELL》 SCIE 2024年第4期525-540,共16页
All tissues in the body are subjected externally to gravity and internally by collagenfibril and cellular retractive forces that create stress and energy equilibrium required for homeostasis.Mechanotransduction involve... All tissues in the body are subjected externally to gravity and internally by collagenfibril and cellular retractive forces that create stress and energy equilibrium required for homeostasis.Mechanotransduction involves mechanical work(force through a distance)and energy storage as kinetic and potential energy.This leads to changes in cell mitosis or apoptosis and the synthesis or loss of tissue components.It involves the application of energy directly to cells through integrin-mediated processes,cell-cell connections,stretching of the cell cytoplasm,and activation of the cell nucleus via yes-associated protein(YAP)and transcriptional coactivator with PDZ-motif(TAZ).These processes involve numerous complexes,intermediate molecules,and multiple pathways.Several pathways have been identified from research studies on vertebrate cell culture and from studies in invertebrates.These pathways involve mechanosensors and other molecules that activate the pathways.This review discusses the mitogen-activated protein kinase(MAPK)family,Hippo,Hedgehog,and Wingless-related integration site(WNT)/βcatenin signaling pathways.The mediators covered includeβcatenin,ion channels,growth factors,hormone receptors,members of the Ras superfamily,and components of the linker of nucleoskeleton and cytoskeleton(LINC)complex.However,the interrelationship among the different pathways remains to be clarified.Integrin-mediated mechanotransduction involves direct tensile loading and energy applied to the cell membrane via collagenfibril stretching.This energy is transferred between cells by stretching the cell-cell connections involving cadherins and the WNT/βcatenin pathway.These alterations induce changes in intracellular events in the cytoskeleton and nuclear skeleton caused by the release of YAP and TAZ.These coactivators then penetrate through the nuclear pores and influence nuclear cell function.Alteration in the balance of forces and energy applied to cells and tissues is hypothesized to shift the cell-extracellular matrix mechanical equilibrium by modifying mechanotransduction.The shift in equilibrium can lead to either tissue synthesis,genetic modifications,or promotefibrotic diseases,including epithelial cell-derived cancers,depending on the local metabolic conditions. 展开更多
关键词 MAPK family HIPPO HEDGEHOG WNT pathway βcatenin Ion channels Growth factor receptors Hormone receptors Ras superfamily LINC complex collagen CADHERINS
下载PDF
Nd:YAG激光辅助治疗牙周炎对患者骨代谢及牙周相关指标的影响 被引量:1
18
作者 龚晓雪 郑涵予 刘春 《中国美容医学》 CAS 2024年第5期137-140,共4页
目的:探究Nd:YAG激光辅助治疗牙周炎对患者骨代谢、牙周相关指标及炎性因子水平的影响。方法:选取2017年1月-2020年4月笔者医院收治的60例牙周炎患者,采用随机数字表法分为对照组和观察组,各30例。对照组采用超声龈下刮治术和根面平整... 目的:探究Nd:YAG激光辅助治疗牙周炎对患者骨代谢、牙周相关指标及炎性因子水平的影响。方法:选取2017年1月-2020年4月笔者医院收治的60例牙周炎患者,采用随机数字表法分为对照组和观察组,各30例。对照组采用超声龈下刮治术和根面平整术进行治疗,观察组在对照组基础上采用Nd:YAG激光辅助治疗,治疗后3个月观察治疗效果。比较两组治疗后3个月总有效率,治疗前、治疗后3个月牙周指标、龈沟液骨钙素(Osteocalcin,OCN)、Ⅰ型胶原(TypeⅠcollagen,COL-Ⅰ)、核因子κB受体活化因子配体(Nuclear factorκB receptor activating factor ligand,RANKL)、炎性因子水平。结果:治疗后3个月,观察组总有效率为96.67%,高于对照组的76.67%(P<0.05)。治疗后3个月,两组牙菌斑指数(Plaque index,PLI)及牙龈指数(Gingival index,GI)评分、牙周袋深度(Pocket depth,PD)水平,龈沟液RANKL、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素6(Interleukin 6,IL-6)、白细胞介素1β(Interleukin 1β,IL-1β)水平较治疗前均降低,且观察组低于对照组(P<0.05);而两组龈沟液OCN、COL-I水平较治疗前升高,观察组高于对照组(P<0.05)。结论:Nd:YAG激光辅助治疗牙周炎可提高患者龈沟液OCN、COL-I水平,降低龈沟液RANKL水平,改善骨代谢,抑制炎症反应,进而改善牙周相关指标,疗效确切。 展开更多
关键词 牙周炎 ND:YAG激光 骨钙素 Ⅰ型胶原 核因子ΚB受体活化因子配体 炎症反应 牙周指标
下载PDF
椎间盘退变中胶原蛋白的代谢失衡
19
作者 董亦直 宋鑫越 +4 位作者 姚鸣宇 朱赫 吴瑞霞 都亚新 祝勇 《中国组织工程研究》 CAS 北大核心 2025年第14期3011-3019,共9页
背景:腰椎间盘退行性疾病是导致腰痛和下肢神经症状的常见疾病。胶原代谢的平衡在维持椎间盘的稳定中发挥着重要的作用。目的:对椎间盘退变中胶原蛋白代谢失衡的研究进展作一综述。方法:由第一作者在中国知网、PubMed、Web of Science... 背景:腰椎间盘退行性疾病是导致腰痛和下肢神经症状的常见疾病。胶原代谢的平衡在维持椎间盘的稳定中发挥着重要的作用。目的:对椎间盘退变中胶原蛋白代谢失衡的研究进展作一综述。方法:由第一作者在中国知网、PubMed、Web of Science数据库中检索2024年5月之前发表的相关文章,中文检索词为“椎间盘退变疾病”“胶原代谢”“胶原酶家族”“胶原合成相关因子”“细胞因子”,英文检索词为“degenerative disc disease”“Collagen metabolism”“Collagenase family”“Collagen synthesis related factors”“Collagen breakdown related factors”。最终纳入76篇文献进行综述。结果与结论:在椎间盘退变的过程中,胶原蛋白代谢的平衡在维持正常椎间盘的稳定中起到至关重要的作用。当椎间盘发生退变时,椎间盘内分泌大量促炎因子、胶原酶,并发生氧化应激反应等,使椎间盘内胶原蛋白分解增加,同时抑制生长因子、胶原蛋白合成酶、胶原蛋合成相关因子的生成,使椎间盘内胶原蛋白合成减少。上述两种情况共同作用使椎间盘内胶原蛋白的代谢平衡被打破,进一步加剧了椎间盘退变的进程。 展开更多
关键词 腰椎间盘退行性疾病 胶原代谢 胶原酶家族 胶原合成相关因子 胶原分解相关因子 综述
下载PDF
IGF-1、β-CTX与胫骨平台骨折术后感染及骨痂X线评分的关系
20
作者 张伟学 王猛 +2 位作者 姜良德 韩奇 王力文 《分子诊断与治疗杂志》 2024年第9期1708-1712,共5页
目的分析血清胰岛素样生长因子-1(IGF-1)、β-Ⅰ型胶原羧基端肽(β-CTX)水平与胫骨平台骨折患者术后感染及骨痂X线评分的关系。方法回顾性分析2020年1月至2023年1月北京市仁和医院收治的90例胫骨平台骨折患者的临床资料,根据术后感染情... 目的分析血清胰岛素样生长因子-1(IGF-1)、β-Ⅰ型胶原羧基端肽(β-CTX)水平与胫骨平台骨折患者术后感染及骨痂X线评分的关系。方法回顾性分析2020年1月至2023年1月北京市仁和医院收治的90例胫骨平台骨折患者的临床资料,根据术后感染情况分为感染组(n=23例)和未感染组(n=67例)。对比两组的临床资料,应用二元Logistic回归模型分析胫骨平台骨折患者术后感染影响因素;采用Spearman相关系数分析IGF-1、β-CTX与术后3个月的骨痂X线评分的相关性,绘制受试者工作特征(ROC)曲线分析IGF-1和β-CTX对患者术后骨折愈合情况的预测价值。结果两组一般资料中性别、损伤机制、骨质疏松情况、合并糖尿病情况、手术时间、联合应用抗生素情况以及住院天数比较差异均无统计学意义(P>0.05);感染组中年龄≥60岁、开放性骨折损伤类型、有遗留死腔的比例均高于未感染组,血清IGF-1和β-CTX水平均低于未感染组,差异有统计学意义(P<0.05);骨痂X线分级Ⅰ~Ⅱ级患者的血清IGF-1和β-CTX水平低于骨痂X线分级Ⅲ~Ⅳ级患者,差异有统计学意义(P<0.05);年龄≥60岁(OR=3.840,95%CI:1.803~8.178)、开放行骨折损伤类型(OR=3.656,95%CI:1.705~7.834)、有遗留死腔(OR=3.407,95%CI:1.670~6.955)、<中位数水平的血清IGF-1(OR=2.578,95%CI:1.388~4.789)和β-CTX(OR=2.550,95%CI:1.262~5.153)的胫骨平台骨折患者术后感染风险更高(P<0.05);血清IGF-1和β-CTX水平与胫骨平台骨折患者术后骨痂X线评分均呈正相关(r=0.671、0.699,P<0.05);血清IGF-1、β-CTX水平及二者联合预测胫骨平台骨折患者术后骨折愈合情况的AUC分别为0.799、0.832、0.884,敏感度分别为0.727、0.727、0.909,特异性分别为0.785、0.798、0.772。结论胫骨平台骨折患者术后感染情况与年龄、损伤类型、遗留死腔、血清IGF-1和β-CTX水平有关,临床可通过检测IGF-1和β-CTX水平变化预测患者术后骨折愈合情况。 展开更多
关键词 血清胰岛素样生长因子-1 β-Ⅰ型胶原羧基端肽 胫骨平台骨折 术后感染 骨痂X线评分
下载PDF
上一页 1 2 59 下一页 到第
使用帮助 返回顶部