ZCCHC10通过激活P53促进急性髓系白血病细胞中CCL18基因的转录

肿瘤抑制因子P53是一种转录因子,可激活一系列靶基因的转录,从而调控细胞周期停滞、DNA修复、免疫、炎症和细胞凋亡等多种生理或病理过程。前期研究表明,CCHC型锌指蛋白10(zinc finger CCHC-type containing 10,ZCCHC10)通过激活P53在肺癌和急性髓系白血病中发挥抑癌作用。为进一步探讨ZCCHC10在急性髓系白血病中的作用机制,本文通过RNA测序(RNA sequencing,RNA-seq)技术对过表达ZCCHC10或空载体的ML2细胞进行了转录组分析,一共鉴定到1284个差异基因[|log2(fold change)|逸1,q值约0.05],包括778个上调基因和506个下调基因。其中,趋化因子CCL18在过表达ZCCHC10的ML2细胞中上调18倍。生物信息学分析显示,CCL18基因启动子上含有两个P53反应元件。生物素标记DNA亲和实验和染色质免疫共沉淀实验证实,P53可结合到CCL18基因启动子上。荧光素酶活性分析表明,P53可以增强CCL18基因启动子的活性。这些研究表明ZCCHC10通过激活P53促进CCL18基因的表达。

Flow Regimes in Bubble Columns with and without Internals: A Review

Hydrodynamics characterization in terms offlow regime behavior is a crucial task to enhance the design of bubble column reactors and scaling up related methodologies.This review presents recent studies on the typicalflow regimes established in bubble columns.Some effort is also provided to introduce relevant definitions pertaining to thisfield,namely,that of“void fraction”and related(local,chordal,cross-sectional and volumetric)variants.Experimental studies involving different parameters that affect design and operating conditions are also discussed in detail.In the second part of the review,the attention is shifted to cases with internals of various types(perfo-rated plates,baffles,vibrating helical springs,mixers,and heat exchanger tubes)immersed in the bubble columns.It is shown that the presence of these elements has a limited influence on the global column hydrodynamics.However,they can make the homogeneousflow regime more stable in terms of transition gas velocity and transi-tion holdup value.The last section is used to highlight gaps which have not beenfilled yet and future directions of investigation.

Comparative impact behaviours of ultra high performance concrete columns reinforced with polypropylene vs steel fibres

Polypropylene(PP) fibres have primarily used to control shrinkage cracks or mitigate explosive spalling in concrete structures exposed to fire or subjected to impact/blast loads, with limited investigations on capacity improvement. This study unveils the possibility of using PP micro-fibres to improve the impact behaviour of fibre-reinforced ultra-high-performance concrete(FRUHPC) columns. Results show that the addition of fibres significantly improves the impact behaviour of FRUHPC columns by shifting the failure mechanism from brittle shear to favourable flexural failure. The addition of steel or PP fibres affected the impact responses differently. Steel fibres considerably increased the peak impact force(up to 18%) while PP micro-fibres slightly increased the peak(3%-4%). FRUHPC significantly reduced the maximum midheight displacement by up to 30%(under 20°impact) and substantially improved the displacement recovery by up to 100%(under 20° impact). FRUHPC with steel fibres significantly improved the energy absorption while those with PP micro-fibres reduced the energy absorption, which is different from the effect of PP-macro fibre reported in the literature. The optimal fibre content for micro-PP fibres is 1% due to its minimal fibre usage and low peak and residual displacement. This study highlights the potential of FRUHPC as a promising material for impact-resistant structures by creating a more favourable flexural failure mechanism, enhancing ductility and toughness under impact loading, and advancing the understanding of the role of fibres in structural performance.

Numerical Analysis of Cold-Formed Thin-Walled Steel Short Columns with Pitting Corrosion during Bridge Construction

Pitting corrosion is harmful during bridge construction,which will lead to uneven roughness of steel surfaces and reduce the thickness of steel.Hence,the effect of pitting corrosion on the mechanical properties of cold-formed thin-walled steel stub columns is studied,and the empirical formulas are established through regression fitting to predict the ultimate load of web and flange under pitting corrosion.In detail,the failure modes and load-displacement curves of specimens with different locations,area ratios,and depths are obtained through a large number of non-linear finite element analysis.As for the specimens with pitting corrosion on the web,all the specimens are subject to local buckling failure,and the failure mode will not change with pitting corrosion,but the failure location will change with pitting corrosion location;the size,location,and area ratio of pitting corrosion have little influence on the ultimate load of cold-formed thin-walled steel short columns,but the loss rate of pitting corrosion section area has a greater impact on the ultimate bearing capacity.As for the specimen with flange pitting corrosion,the location and area ratio of pitting corrosion have less influence on the ultimate load of cold-formed thin-walled steel short columns,and the section area loss rate has greater influence on the ultimate bearing capacity;the impact of web pitting corrosion on the ultimate load is greater than that of flange pitting corrosion under the same condition of pitting corrosion section area.The prediction formulas of limit load which are suitable for pitting corrosion of web and flange are established,which can provide a reference for performance evaluation of corroded cold-formed thin-walled steel.

Modelling and Sizing of a Floor Reinforced by Ballasted Columns Intended to Support a Tank

This work aims to study the modeling and sizing of a floor reinforced by ballasted columns. We are studying the system of reinforcement by ballasted columns because this technique is able to replace deep foundations that are technically difficult to realize and their cost is higher. The modelling and dimensioning of foundations on a ballasted column will be an important contribution to the state of the art of this method because it will highlight the mode of transfer of loads, and will expose the induced deformations by also allowing to verification criteria of bearing capacity and allowable settlement according to geometric information of the model. The columns on a substrate located at 9 m have a length of 9 m and a diameter of 40 cm and were obtained by incorporating ballast of granular class 0/31.5 of internal friction angle of 38˚ and a density weight of 21 kN/m3. The choice of this method is based on the geotechnical characteristics of the initial soil. Thus, identification and characterization tests were carried out to estimate the bearing capacity and the settlement giving respectively 125 kPa and 57 cm. These results show the ground does not have sufficient mechanical properties to withstand the loads transmitted by the tank. By adopting the reinforcement of the soil with ballasted columns, numerical calculations show that after applying a load equal to 265.1 KPa, 20 cm vertical settlement and 17 cm horizontal displacement were obtained. This is in the tolerable deformation range for our tank, namely, less than 20 cm. Analytically, in addition to reducing settlement, ballasted columns, Due to their high stiffness, they have effectively contributed to the increase of the permissible soil stress up to 257 kPa.

Bubble size modeling approach for the simulation of bubble columns

The constant bubble size modeling approach(CBSM)and variable bubble size modeling approach(VBSM)are frequently employed in Eulerian–Eulerian simulation of bubble columns.However,the accuracy of CBSM is limited while the computational efficiency of VBSM needs to be improved.This work aims to develop method for bubble size modeling which has high computational efficiency and accuracy in the simulation of bubble columns.The distribution of bubble sizes is represented by a series of discrete points,and the percentage of bubbles with various sizes at gas inlet is determined by the results of computational fluid dynamics(CFD)–population balance model(PBM)simulations,whereas the influence of bubble coalescence and breakup is neglected.The simulated results of a 0.15 m diameter bubble column suggest that the developed method has high computational speed and can achieve similar accuracy as CFD–PBM modeling.Furthermore,the convergence issues caused by solving population balance equations are addressed.

Seismic behavior of steel tubular bridge columns equipped with low-yield-point steel plates in the root replaceable pier

To enable rapid recovery of a steel bridge column after an earthquake,a novel tubular-section steel bridge column equipped with low-yield-point(LYP)steel tubular plates in the root replaceable pier is proposed.For the purpose of discussing the seismic behavior of the novel steel bridge column,quasi-static tests and finite element simulation analyses of the specimens were carried out.The effects of parameters such as the axial compression ratio,eccentricity,and thickness and material strength of the tubular plate in the energy-dissipating zone are discussed.Experimental results from seven specimens that were subjected to four failure modes are presented.The damage to the quasi-static specimens is localized to the replaceable energy-dissipating pier.The seismic behavior of the novel steel bridge columns is significantly influenced by the axial compression ratio and eccentricity of specimens.Numerical results show that the high stress area of the specimens is mainly concentrated in the connection zone between the LYP steel tubular plate and the bottom steel plate,which is consistent with the position of the quasi-static specimen when it was prone to fracture.Finally,a calculation formula is proposed to facilitate the capacity prediction of this new steel tubular bridge column under repeated loading.

Configuring topologically optimum vapor recompressed dividing-wall distillation columns to maximize operating efficiency

For dividing-wall distillation columns(DWDCs) separating a heavy-component dominated and wide boiling-point ternary(HCDWBT) mixture, a significant amount of excessive heat exists inevitably in stripping the heavy-component from the intermediate-component and it can be employed to initiate the development of vapor recompression heat pump(VRHP) assisted DWDC(VRHP-DWDC). Despite dividing wall may locate in the top, middle, and bottom, the optimum VRHP-DWDC is found to involve uniformlytwo VRHP circles. While the first one serves to compress and transform the excessive heat resulted from the separation of the heavy-component from the intermediate-component, the second one to compress and transform the overhead vapor stream of the light-component pre-heated sequentially with the condensate from the first one and the bottom product stream of the heavy-component, both releasing the temperature-elevated latent heat to the pre-fractionator's or common stripping section. The processing of two HCDWBT mixtures of benzene/toluene/o-xylene and n-pentane/n-hexane/n-heptane are selected to assess the derived optimum topological configurations of the VRHP-DWDC and their optimality is confirmed through detailed comparisons with the DWDC and two VRHP-DWDCs involving only one VRHP circle. The proposed strategy helps to tap the full potential of the VRHP-DWDC with considerably alleviated complication in process development.

Gamma Photon Column Scanning of Prototype and Industrial Distillation Columns

Monte Carlo simulation of gamma photon transport and interaction with the distillation column and its contents was performed in order to predict the effects of gamma photons when they interact with matter. The results of the interaction and transport of gamma photons are presented as energy deposition on the distillation column and its contents. Energy attenuation was more pronounced on the column walls and trays as compared to the region between the trays, where there is mostly vapour space. Gamma column scanning was then used to verify the Monte Carlo simulation results by scanning and investigating the integrity of two laboratory prototype distillation and industrial distillation columns. One of the prototype distillation columns was 1 m tall with four trays and the other one was 1.8 m tall consisting of six trays and a packed bed. Commonly encountered distillation column malfunctions such as collapsed tray, weeping, flooding and foaming were simulated in the two prototype distillation columns and scanned. The industrial distillation column was a 26 m tall benzole prefractionator column, consisting of 60 single pass trays and a diameter of 0.8 m. A 10 mCi 60Co gamma radiation source and NaI(Tl) scintillation detector were used to scan the distillation columns. The results from the two prototypes showed that all the simulated malfunctions were clearly detected except for foaming. The results from industrial distillation column showed that all the trays were in their correct position although tray number 32 could be partially damaged and just below tray 41, the scan revealed that there was a loss of column wall thickness. The obtained density profile for the industrial distillation column showed some small variations from the expected density profile and this was attributed to external features on the distillation column and wind bursts that shifted the source and detector from the chosen scan line orientation.

An Experimental Study of Composite Columns Filled with Eucalyptus nitens Timber under Axial Compression

Eucalyptus nitens(E.nitens)has been much used for producing paper but also shows promise for structural applications.In this study,static compressive tests were undertaken to examine its suitability to be used in an innovative composite column.The composite column was comprised of a rectangular steel tube with E.nitens timber infill.The nonlinear compressive behaviour of the composite column filled with E.nitens wood for both dry and wet conditions was examined.The same tests on rectangular steel tubes and bare dry and wet E.nitens samples were also undertaken as a comparison.For samples with different conditions,the ultimate capacity was evaluated and the effect of each condition on the compressive behaviour of the composite column was clarified.The steel tubes showed greater ductile behaviour,and more ductility was found in the wet samples.The steel tubes with E.nitens timber infill samples exhibited a greater linear elastic range connected with higher maximum loads,while the bare timber samples could support only lower maximum loads.The results from this research were promising for the use of rectangular steel tubes with E.nitens timber infill in structural applications.

Synthesis and Design of Distillation Columns for Wide-Boiling Binary Mixtures with Vapor Recompression Heat Pumps

The vapor recompression heat pump(VRHP) distillation technology offers significant improvements in energy efficiency for distillation systems with small temperature differences between the top and bottom of the column. However, the separation of wide-boiling binary mixtures leads to substantial temperature differences between the top and bottom of the column. This limits the applicability of conventional VRHP due to high capital costs and strict performance requirements of the compressor. To overcome these challenges and to accommodate compressor operating conditions, a novel synthesis and design method is introduced to integrate VRHPs with wide-boiling binary mixture distillation columns(WBMDCs). This method enables quick determination of an initial configuration for the integrated WBMDC-VRHP system and helps identify the optimum configuration with the minimum total annual cost. Two examples, namely the separation of benzene/toluene and isopropanol/chlorobenzene, are employed to derive optimum configurations of the WBMDC-VRHP and compare them with the WBMDC. A systematic comparison between the WBMDC-VRHP and WBMDC demonstrates the superior steady-state performance and economic efficiency of the WBMDC-VRHP.

An accurate detection algorithm for time backtracked projectile-induced water columns based on the improved YOLO network

During a sea firing training,the intelligent detection of projectile-induced water column targets in a firing video is the prerequisite for and critical to the automatic calculation of miss distance,while the correct and precise calculation of miss distance is directly affected by the accuracy,false alarm rate and time delay of detection.After analyzing the characteristics of projectile-induced water columns,an accurate detection algorithm for time backtracked projectile-induced water columns based on the improved you only look once(YOLO)network is put forward.The capability and accuracy of detecting projectileinduced water column targets with the conventional YOLO network are improved by optimizing the anchor box through K-means clustering and embedding the squeeze and excitation(SE)attention module.The detection area is limited by adopting a sea-sky line detection algorithm based on gray level co-occurrence matrix(GLCM),so as to effectively eliminate such disturbances as ocean waves and ship wakes,and lower the false alarm rate of projectile-induced water column detection.The improved algorithm increases the mAP50 of water column detection by 30.3%.On the basis of correct detection,a time backtracking algorithm is designed with mean shift to track images containing projectile-induced water column in reverse time sequence.It accurately detects a projectile-induced water column at the time of its initial appearance as well as its pixel position in images,and considerably reduces detection delay,so as to provide the support for the automatic,accurate,and real-time calculation of miss distance.

Experimental Behavior of Recycled Aggregate Concrete Filled Steel Tubular Columns

During the modernization or rehabilitation activity,the demolished structural waste causes large soil pollution and unavailability of natural aggregate is the big concern for the construction industry.Therefore,this manuscript deals with the Composite Steel Circular Column(CSCC)with Recycled Aggregate concrete(RAC)as infill is partly used,with the replacement of 25%and 50%in M30 grade of Concrete.And internal reinforcement steel is fully replaced by rolled steel tubes(circular and square)with varied thickness,ISA-unequal angle.Around 14 specimens are cast and examined under axial load for analysis of the deflection characteristics,the load-bearing capacity along with its buckling behavior.The experimental values are estimated through LVDT(linear variable differential transducer)at 3-phase.The curve of load-deflection is drawn with the load pattern.From the date interpretation,it is found column made of 50%-RAC has more than 25%-RAC.

某难处理金矿生物预氧化

某金矿石中黄铁矿含量5.31%、氧化率6.02%,直接氰化金浸出率仅27.78%,属典型的低品位硫化物包裹型难处理金矿。为评估生物堆浸预氧化工艺对该矿石的工业化应用前景,开展了直接氰化试验、生物搅拌预氧化-氰化试验和生物柱浸预氧化-氰化试验,考察了黄铁矿氧化率和金浸出率的关系以及温度对黄铁矿氧化率和金浸出率的影响。生物柱浸试验获得良好指标:原生矿破碎至D80=5.5 mm,在室温条件下(8~30℃)预氧化221 d后,黄铁矿氧化率52.30%,金的浸出率为62.70%,氰化渣金品位为0.47 g/t,较直接氰化浸出金浸出率(27.78%)提高34.92个百分点。

新型承插式螺栓连接柱-柱节点抗拉性能研究

为实现模块化钢结构单元房间的上下连接,提出一种新型承插式螺栓连接柱-柱节点,以有无注浆、承插深度为参数设计并制作3个足尺节点试件,并对其进行抗拉试验,分析了各节点的破坏形态、应变分布以及承载能力等,探讨了该新型节点的抗拉性能.建立了数值分析模型,进行了轴拉荷载作用下的受力性能参数化分析,研究了承插深度、螺栓直径及内套筒厚度对节点抗拉承载力的影响.基于高强螺栓的抗剪承载力,提出了适用于该新型节点的抗拉承载力计算公式.研究结果表明:该新型节点可将轴向拉力有效传递至高强螺栓,试件破坏时均出现高强螺栓群被剪断,灌浆节点试件发生破坏时,出现钢材与灌浆料界面的黏结破坏及螺栓周围局部灌浆料的压碎;高强螺栓群在拉力荷载作用下呈端部螺栓受剪较大、中心螺栓受剪较小的分布,试件破坏时,各螺栓承受的剪力趋于相等;灌浆节点与无浆节点相比,灌浆料与高强螺栓协同工作性能良好,弹性阶段最大摩擦力平均值提高64.4%,极限抗拉承载力提高14.1%;承插深度由300 mm增至500 mm,节点极限抗拉承载力提高80.9%;承插深度和螺栓直径对节点抗拉承载力影响较大,内套筒厚度对节点抗拉承载力的影响较小;根据提出的节点抗拉承载力计算公式得到的计算值与有限元模拟值吻合良好.

局部锈蚀圆钢管混凝土短柱轴压承载力试验研究

钢管混凝土(CFST)在服役环境中被腐蚀,导致其钢结构承载力降低,严重威胁到结构的服役性能和使用寿命。首先,采用机加工车铣方法制作模拟局部锈蚀的人工缺陷,然后以钢管外表面局部环向贯通锈蚀位置、锈蚀钢管体积损失率、锈蚀外表面面积损失率(简称锈蚀面积损失率,下同)为试验参数,对45根局部锈蚀圆CFST短柱试件进行轴压承载力试验;其次,分析锈蚀位置、锈蚀钢管体积损失率、面积损失率和壁厚损失率对锈蚀试件承载力、刚度和延性的影响,揭示锈蚀CFST试件破坏机理和承载力退化机制;最后,针对局部锈蚀圆CFST短柱构件轴压承载力提出一个简化实用计算公式。研究结果表明:各试件具有类似的破坏特征,主要呈明显的腰鼓状破坏,且发生在锈蚀区;随着锈蚀钢管体积损失率增大,锈蚀CFST柱的承载力、刚度和延性均出现不同程度的降低;在锈蚀钢管体积损失率和面积损失率相同的情况下,就局部锈蚀位置影响而言,中部影响最大;就锈蚀程度表征参数影响而言,锈蚀钢管体积损失率影响最大,面积损失率次之,壁厚损失率最小;本文提出的简化实用公式可为圆钢管混凝土构件全寿命设计提供参考依据。

3D打印混凝土永久模板叠合柱的抗压性能数值模拟研究

为深入研究3D打印混凝土永久模板叠合柱的抗压性能,基于3D打印混凝土永久模板叠合柱及同尺寸整体现浇对照柱试验建立构件数值模型,模拟分析其轴压荷载-位移响应及失效形态。针对界面粘结性能、现浇混凝土抗压强度、打印模板厚度、荷载偏心距等参数开展3D打印混凝土永久模板叠合柱的抗压性能计算分析,研究表明:叠合柱轴压极限承载力随着薄弱界面剪切强度、刚度及现浇混凝土抗压强度的增大而增大。由于打印材料的抗压强度高于现浇混凝土,叠合柱抗压极限承载力提升率与打印模板厚度呈近似线性关系,叠合圆柱的抗压极限承载力随着荷载偏心距的增大而降低,呈近似线性负相关。此外,偏心距对叠合圆柱极限承载力下降幅度的影响大于现浇圆柱。

微细粒矿物浮选综述:增大颗粒表观尺寸与减小气泡直径

微细粒矿物的浮选回收是世界性难题,增大颗粒表观直径与减小气泡尺寸为解决该难题的有效途径。论文综述了增大颗粒表观直径的四种方法:疏水絮凝浮选、载体浮选、选择性絮凝浮选和剪切絮凝浮选,详细阐述了其在矿物加工领域中的应用及机理,尤其是增大颗粒表观粒径过程中新药剂的最新研究进展及应用领域。从减小气泡尺寸角度出发,以微纳米气泡在矿物加工领域的应用研究为落脚点,阐述了微纳米气泡现有的稳定性机理,为后续微纳米气泡稳定性机理的深入研究提供参考;系统介绍了微纳米气泡在不同种类微细粒矿物浮选中的应用现状;从微纳米气泡与颗粒间界面作用机理出发,详细阐述了微纳米气泡在界面作用中的角色;举例介绍了微纳米气泡浮选设备的研究进展。提出微纳米气泡强化细粒浮选的机理需要进一步明确,基于微纳米气泡、矿浆精准可控的微纳米气泡浮选设备是微细粒矿物浮选的重要研究方向。

深埋岩溶隧道爆破开挖对围岩损伤及渗流特性的影响

深埋岩溶隧道开挖会造成围岩特殊的损伤破坏形式,并且损伤区也会影响围岩渗流场及隧道边界的涌水状况,对深埋岩溶隧道爆破开挖作用下围岩的损伤及渗流特性有重要的研究意义。为了研究爆破开挖对深埋岩溶隧道围岩损伤及渗流的影响规律,基于COMSOL Multiphysics软件建立数值模型并嵌入应力-渗流-损伤耦合方程式进行计算,采用解析法计算了简化条件下岩溶隧道开挖时围岩的应力分布,并与数值计算结果进行比较。结果表明:解析法与数值法计算的围岩应力分布有一致性,爆炸荷载使靠近溶洞侧隧道肩部及脚部区域会产生较大的拉应力;爆破后会在隔水岩柱形成“齿”状损伤区,同时引起肩部及脚部区域流速的增加,其可作为判断溶洞方位的参考依据;溶洞净距减小、洞径及水压增加会引起“齿”延伸倾角及最大涌水位置变化、隧道边界涌水量的增加,可根据“齿”延伸方向判断损伤区检测的合理方位,根据最大涌水位置变化、隧道边界涌水量的变化,合理调整涌水防治措施强度及重点防治部位。研究成果可为溶洞位置判定、隧道损伤区检测以及边界涌水防治措施提供参考。

一次强冰雹超级单体风暴双偏振参量特征分析

利用济南S波段双偏振多普勒天气雷达资料、章丘探空和地面常规气象观测资料及灾情调查,对2021年7月9日发生在济南章丘的一次特大冰雹超级单体风暴双偏振和微物理结构特征进行了分析。结果表明:冷涡天气背景下,强的垂直风切变和强的对流有效位能,利于超级单体的形成与维持。阵风锋是风暴触发机制,也是风暴长时间维持机制。初始风暴由阵风锋触发,经过合并发展产生超级单体。成熟阶段,风暴西侧与阵风锋交汇区域不断激发新生单体,并与主体合并,风暴长时间维持。风暴顶强辐散是中气旋长时间维持和风暴顶高度较高的关键因子之一。特大冰雹阶段风暴底层右后有明显的入流缺口,其前侧有差分反射率(Z_(DR))弧,表现为少量大的液态粒子或小的湿冰粒子,入流缺口左侧强反射率因子区对应小的Z_(DR)和小的相关系数,为冰雹降落区。垂直结构上,强上升气流区一侧存在深厚的有界弱回波区,0℃层高度之下分布有Z_(DR)环,有界弱回波区内及上方存在Z_(DR)柱,且高度较高,含有少许偏大的液态或融化的小的冰相粒子。较高的Z_(DR)柱表明风暴内上升气流强盛且高度较高,利于风暴的发展与维持以及冰雹粒子的湿增长。