As important atmospheric circulation patterns in Northern Hemisphere(NH),the North Atlantic Oscillation(NAO)and the Western Pacific teleconnection(WP)affect the winter climate in Eurasia.In order to explore the combin...As important atmospheric circulation patterns in Northern Hemisphere(NH),the North Atlantic Oscillation(NAO)and the Western Pacific teleconnection(WP)affect the winter climate in Eurasia.In order to explore the combined effects of NAO and WP on East Asian(EA)temperature,the NAO and WP indices are divided into four phases from 1980−2021:the positive NAO and WP phase(NAO+/WP+),the negative NAO and WP phase(NAO−/WP−),the positive NAO and negative WP phase(NAO+/WP−),the negative NAO and positive WP phase(NAO−/WP+).In the phase of NAO+/WP+,the low geopotential height(GH)stays in north of EA at 50°−80°N;the surface air temperature anomaly(SATA)is 0.8−1℃lower than Southern Asian.In the phase of NAO−/WP−,the center of high temperature and GH locate in the northeast of EA;the cold air spreads to Southern Asia,causing the SATA decreases 1−1.5℃.In the phase of NAO+/WP−,the high GH belt is formed at 55°−80°N.Meanwhile,the center of high SATA locates in the north of Asia that increases 0.8−1.1℃.The cold airflow causes temperature dropping 0.5−1℃in the south of EA.The SATA improves 0.5−1.5℃in south of EA in the phase of NAO−/WP+.The belt of high GH is formed at 25°−50°N,and blocks the cold air which from Siberia.The NAO and WP generate two warped plate pressure structures in NH,and affect the temperature by different pressure configurations.NAO and WP form different GH,and GH acts to block and push airflow by affecting the air pressure,then causes the temperature to be different from the north and south of EA.Finally,the multiple linear regression result shows that NAO and WP are weakened by each other such as the phase of NAO+/WP+and NAO−/WP−.展开更多
In this study,we have examined the effects of exposure to high temperature, carbon inonoxideor a combination of both conditions in a model system,the rat and in industrial workers.In the rat liver, HSP70 mRNA and HSP7...In this study,we have examined the effects of exposure to high temperature, carbon inonoxideor a combination of both conditions in a model system,the rat and in industrial workers.In the rat liver, HSP70 mRNA and HSP70 synthesis were measured by dot hybridization and western blot. The results showed that after a heat stress HSP70 mRNA and its product, HSP70 increased significantly and there was a synergism in the combined effects of high temperature and carbon monoxide exposure on the induction of HSP70 mRNA and HSP70 synthesis. Heat played a major role in this induction. The presence of antibodies to human HSP27, HSP60, HSP70,HSC73, HSP89 αand β in workers exposed to heat, carbon monoxide was also measured by western blot using purified HSPs as antigens. Plasma free amino acids were measured in the saine group of workers. The incidence of antibodies to HSP27 and HSP70 Was significantly higher in the workers working in an environment with extreme heat, and high carbon monoxide ernission than in a control group. The carbon monoxide exposed group showed the highest incidence of antibodies to HSPs. Although our previous results indicated that workers had an insufficient protein intake,plasma free amino acids tended to increase, especially in methionine and tryptophan two kinds of amino acids which are absent from the main stress protein, HSP70.These results suggest that the major problems that these workers may face are how to facilitate the use of plasma free amino acids and reduce the inhibition of synthesis of normal proteins when they are exposed to occupational harmful factors.These resultsalso add new information on the measurement of HSPs as a potential biomonitor to assess whether organisms are experiencing metabolic stress within their environment.展开更多
The hydraulic concrete durability under the alternation of freeze-thaw and carbonation has been systematically investigated in this work, where both the micro part and the microscopic characteristics of concrete inter...The hydraulic concrete durability under the alternation of freeze-thaw and carbonation has been systematically investigated in this work, where both the micro part and the microscopic characteristics of concrete interface were analyzed based on computed tomography(CT) test and scanning electron microscopy(SEM). Average CT numbers of each section, declined at water-cement ratio of 0.35, increased at 0.45, and changed a little at 0.55. The specimen in the absence of fly ash exhibited less types of hydration products and the surface was observed to be a needle-like ettringite, with a relatively dense overall structure. However, with the increase of fly ash content, pores and micro-cracks of specimen structure increased. Hexagonal flake calcium hydroxide, present in the specimen after the first carbonation, was negligible in the test pieces of the first freezethaw where the main hydration products were ettringite and calcium silicate gel. Regular hexagonal plates of calcium hydroxide exhibited in the interior of the specimen in which charring first occurred but calcium hydroxide rarely existed in the interior of the specimen in which freeze-thaw first occurred.展开更多
Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainl...Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18–34°C) and copper ion concentration(0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P〈0.01), the quadratic effect of temperature was significant(P〈0.05); the linear effect of copper ion concentration was not significant(P〉0.05), while the quadratic effect of copper ion concentration was highly significant(P〈0.01); the interactive effect of temperature and copper ion concentration was not significant(P〉0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.展开更多
The influences of the calmodulin antagonist chlorpromazine (CPZ), and calcium channel blocker nimodipine (NIMO) and their combination on cadmium (Cd) poisoning of mice were studied. A seties of biochemical parameters...The influences of the calmodulin antagonist chlorpromazine (CPZ), and calcium channel blocker nimodipine (NIMO) and their combination on cadmium (Cd) poisoning of mice were studied. A seties of biochemical parameters including urinary enzyme activities, blood and urine Cd levels, metallothionein (MT) contents in liver and kidney, hepatic ultrastructure and Ca2+ -Mg2+ AT-Pase activity in erythrocyte membrane were determined. Animal models for Cd poisoning were established by peritoneal injection of 1/5 LD50 CdCl2. The experimental groups were protected by administration of CPZ, NIMO and CPZ and NIMO in combination l h before the injection of CdCl2. Five days later, samples were collected for analysis. The data showed that Crs could protect kidney tissue against Cd-induced damage, as the urinary γ-glutamyl traspepti dase (γ- GT ) and N- acetyl-β-D-glucosaminidase (NAG) activities were reduced significantly. There was neither evidence of the protective effect of NIMO on kidney tissue nor an indication of a synergistic effecf of Crs and NIMO.Both CPZ and NIMO showed a considerable protective effect against the deerease in Ca2+ -Mg2+ AT-Pase activity, and a synergistic action was observed. Cd content in blood was reduced significanily by CPZ or the combination of CPZ and NIMO, but elevated by NIMO. Both CPZ and NIMO consideraby increased MT contents in livers and kidneys and ameliorated damaged to the hepatic ultrastructures caused by Cd. The results indicated that these inhibitors could protect mice against the toxic effects of Cd in liver and kidney tissues, while CPZ was more efficient than NIMO. The combination of CPZ and NIMO exerted a synergistic action. The protective action of these two drugs might be relevent to the function of MT.展开更多
Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes agai...Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B(UVB)irradiation remain unclear.This study was undertaken to explore the effects of combination of 1,25(OH)_(2)D_(3) and TAK-242(TLR-4 inhibitor)on the damage to HaCaT cells caused by UVB irradiation.Methods In vitro,HaCaT cells were treated with 1,25(OH)_(2)D_(3) or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm^(2),then the production of reactive oxygen species(ROS),cell migration,apoptosis of cells,and the expression of oxidative stress,endoplasmic reticulum stress,and apoptosis related proteins were determined.Results Compared with the HaCaT cells treated with 1,25(OH)_(2)D_(3) or TAK-242,the cells treated with both 1,25(OH)_(2)D_(3) and TAK-242 showed,1)significantly lower production of ROS(P<0.05);2)significantly less apoptosis of HaCaT cells(P<0.05);3)significantly lower expression of NF-κB,Caspase-8,Cyto-C,Caspase-3(P<0.05).Conclusion The combination of 1,25(OH)_(2)D_(3) and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress,endoplasmic reticulum stress and apoptosis than 1,25(OH)_(2)D_(3) or TAK-242 alone.展开更多
BACKGROUND: Central adrenergic nerve and 5-serotonergic nerve can influence central cholinergic nerve on learning and memory and make easy for study; however, ginsenoside of stem and leaf (GSL) can improve function...BACKGROUND: Central adrenergic nerve and 5-serotonergic nerve can influence central cholinergic nerve on learning and memory and make easy for study; however, ginsenoside of stem and leaf (GSL) can improve functions of central adrenergic nerve; moreover, 5-serotonergic nerve and the combination with choline can produce synergistic effect and enhance learning and memory ability so as to improve learning and memory disorder of patients with Alzheimer disease (AD). OBJECTIVE : To observe the effects of GSL combining with choline on learning and memory of AD model rats DESIGN : Randomized grouping design and controlled animal study SETIING : Department of Pharmacology, Taishan Medical College MATERIALS : The experiment was carried out in the Pharmacological Department of Medical College of Jilin University from October 1996 to January 1997. Forty healthy male Wistar rats of clean grade were randomly divided into 5 groups, including sham-injury group, model group, GSL group, choline group and combination group, with 8 rats in each group. Main medications: GSL with the volume more than 92.8% was provided by Department of Chemistry, Norman Bethune Medical College of Jilin University. Panaxatriol, the main component, was detected with thin layer scanning technique and regarded as the index of GSL quality [(55±1)%, CV= 2%, n = 5]. Choline was provided by the Third Shanghai Laboratory Factory. METHODS : 150 nmol quinolinic acid was used to damage bilateral Meynert basal nuclei of adult rats so as to establish AD models. Rats in GSL, choline and combination groups were intragastric administrated with 400 mg/kg GSL, 200 mg/kg choline (20 mL/kg), and both respectively last for 17 days starting from two days before operation. Rats in sham-injury group and model group were perfused with the same volume of distilled water once in each morning for the same days. (1) Passive avoidance step-down test: Five minutes later, rats jumped up safe platform when they were shocked with 36 V alternating current. If rats jumped down from the platform and the feet touched railings, the response was wrong. Numbers of wrong response were recorded within 3 minutes, and then the test was redone after 24 hours. (2) Morris water-maze spatial localization task: Swimming from jumping-off to platform directly was regarded as right response. Additionally, 4 successively right responses were regarded as the standard. Each rat was trained 10 times a day with 120 s per time for 3 successive days. The interval was 30 s. Three days later, numbers of right response were recorded. The training times were increased to 30 for unlearned rats. (3) Measurement of activity of choline acetylase in cerebral cortex: Rats were sacrificed at 17 days after operation to obtain cerebral cortex to measure activity of choline acetylase with radiochemistry technique. (4) Synergistic effect: It was expressed as Q value: Q value = factual incorporative effect/anticipant incorporative effect; Q ≥ 1 was regarded as synergistic effect. Anticipant incorporative effect = (EA+EB-EA·EB), EA and EB were single timing effect, respectively in GSL group and choline group. E(step-down test and Morris water maze test) = (x in model group - factual value in medicine groups)/x in model group; E (activity of choline acetylase) = (factual value in medicine groups -xin model group)/xin model group. MAIN OUTCOME MEASURES : (1) Passive avoidance step-down test and Morris water-maze spatial localization task in the study of learning and memory; (2) activity of choline acetylase. RESULTS : All 40 rats were involved in the final analysis. (1) Passive avoidance response: At learning phase on first day and retesting phase on the next day, numbers of wrong responses within 3 minutes were more in model group than sham operation group, and there was significant difference [(5.88±1.46), (2.25±0.87) times; (2.63±1.06), (0.50±0.53) times; P 〈 0.01]; numbers of wrong responses within 3 minutes were less in combination group than model group, and there was significant difference [learning phase: (1.12±0.83), (5.88±1.46) times; retesting phase: (0.38±0.74), (2.63±1.06)times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and 1.59, respectively and it showed synergistic effect. Spatial localization task: Training times were more in model group than sham operation group, and there was significant difference [(2.9±2.5), (12.6±3.5) times; P 〈 0.01]. Training times were less in combination group than model group, and there was significant difference [(11.8±2.4), (27.9±2.5) times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and it showed synergistic effect. (3) Activity of choline acetylase: Activity was lower in model group than sham operation group, and there was significant difference [(30.56±8.33), (61.11 ±8.33) nkat/g; P 〈 0.01]. Activity was higher in combination group than model group and there was significant difference [(50.00±8.33), (30.56±8.33) nkat/g, P 〈 0.01];moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.5 and it showed synergistic effect. CONCLUSZON: GSL in combination with choline can synergically improve the disorder of learning and memory of AD model rats. Its mechanism may be involved in enhancing the function of central cholinergic system.展开更多
The effect of the Chinese herbal compound (CHC) on solid sarcoma 180 (S180) in Swiss mice was studied either alone or in combination with 5-fluorouracil (5FU), cyclophosphamide (CYT) or mitomycin C (MMC). The prelimin...The effect of the Chinese herbal compound (CHC) on solid sarcoma 180 (S180) in Swiss mice was studied either alone or in combination with 5-fluorouracil (5FU), cyclophosphamide (CYT) or mitomycin C (MMC). The preliminary results indicated that combination treatment seemed to possess better antitumor activity than chemotherapy alone. The treatment with CHC alone however had neither an obvious antitumor effect in tumor bearing mice nor toxicity in normal mice. These results show that CHC may stimulate organs of the immune system such as the spleen to be im-munomodulators and enhance the antitumor activity of some chemotherapeutic agents.展开更多
The effects of bleomycin A5 (BLM A5) alone and combined with calmodulin inhibitor N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide (W-13) on the proliferation on S-180 cells in vitro were studied. IC50 of BLM used ...The effects of bleomycin A5 (BLM A5) alone and combined with calmodulin inhibitor N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide (W-13) on the proliferation on S-180 cells in vitro were studied. IC50 of BLM used alone for the cells was about 2.63 μg/ml, but it was reduced to 1/3.8 and 1/9.5 of 2.63 μg/ml when plus W-13 1, 5 μg/ml respectively. The results indicated that nontoxic doses of W-13 enhanced the hinibition of cell proliferation under the condition of BLM 0.5 - 2.5 μg/ ml. In colony forming test, the survival fraction of S-180 cells treated with BLM plus W-13 was decreased to 1/87 - 240 of that of the cells treated with BLM alone. The results suggest that W-13 can enhance antitumor activity of BLM in vitro and may be used as an synergist of BLM A5 in vivo.展开更多
To investigate the effects of patient-controlled intravenous analgesia (PCIA) with lornoxicam and fentanyl on arrhythmia and the expression of platelet membrane glycoproteins in patients with coronary artery disease (...To investigate the effects of patient-controlled intravenous analgesia (PCIA) with lornoxicam and fentanyl on arrhythmia and the expression of platelet membrane glycoproteins in patients with coronary artery disease (CAD) after abdominal surgery.Methods Eighty ASA Ⅱ or Ⅲ patients with CAD aged 51~66 yrs weighing 59~68 kg presenting for abdominal surgery participated in this study.CAD was diagnosed by clinical symptoms and ischemic changes on ECG.The patients were premedicated with intramuscular henobarbital 0.1 g and scopolamine 0.3 mg.Anesthesia was induced with fentanyl,droperidol,propofol and vecuronium and maintained with propofol,fentanyl and vecuronium.The patients received PCIA after operation.The PCIA solution contained fentanyl 0.9 mg and droperidol 5 mg in 100 ml of normal saline (N.S.) in group A (n=40) or lornoxicam 56 mg,fentanyl 0.2 mg and droperidol 5 mg in 100 ml N.S. in group B (n=40).In group A the loading dose was fentanyl 0.05 mg and group B lornoxicam 4 mg.PCIA included a background infusion at 2 ml·h -1 and a bolus of 0.5 ml with a 15 min lock-out.VAS(0=no pain,10= worst pain) was used to measure pain intensity.In addition to BP,HR and SpO2 monitoring ECG was continuously monitored with a Holter monitor after operation.Blood samples were taken from peripheral vein before and 6 h after operation and on the 1st,2nd,7th and 8th postoperative days for determination of the expression of CD 62p ,CD 63 and CD 41 /CD 61 on the platelet membrane,platelet count,prothrombin time (PT) thrombin time (TT) and partial thromboplastin time (PTT).Results The two groups were comparable with respect to sex,age,body weight,severity of CAD,duration of operation and intraoperative blood loss.The patients received no blood transfusion during operation.There was no significant difference in VAS score,platelet count,PT,TT and PTT between the two groups.The incidence of atrial and ventricular premature beat on ECG and the expression of CD 41 /CD 61 ,CD 62p and CD 63 on the platelet membrane were significantly lower in group B than in group A on the 7th and 8th postoperative days(P<0.05 or 0.01).Conclusion Postoperative PCIA with lornoxicam and fentanyl can more effectively reduce the incidence of postoperative arrhythmia in patients with CAD.Suppression of activation of platelets by lornoxicam may contribute to the mechanism.10 refs,3 tabs.展开更多
It has been confirmed that microplastics(MPs)are present in the environment.This study simulated secondary PE-MPs via aging and mechanical processes to evaluate their effects on Pak choi(Brassica rapa L.)over 21 d.Two...It has been confirmed that microplastics(MPs)are present in the environment.This study simulated secondary PE-MPs via aging and mechanical processes to evaluate their effects on Pak choi(Brassica rapa L.)over 21 d.Two common pollutants,dichlorodiphenyltrichloroethane(DDT)and naphthalene,were used in the combined toxicity tests.The results indicated that the growth of Pak choi was significantly inhibited after exposure to secondary PE-MPs,and the combined effects were antagonistic,owing to the adsorption capacity of secondary PE-MPs to DDT and naphthalene.Oxidative stress in Pak choi can be markedly affected,leading to oxidative damage to plant cells.The moisture content,soil bulk density,soil density,cation exchange capacity(CEC),and FDA hydrolase in the planted soils increased in the treated groups,and the TOC content changed significantly.We also found that the microbial composition of the soil in the DDT and naphthalene groups showed more significant alterations than that in the other groups.Alpha diversity analysis showed that species diversity increased in the combined groups but indicated a clear downward trend in the single MPs groups.This study suggests that secondary PE-MPs harm the growth of Pak choi and can change soil properties,revealing the harm to the ecosystem of MPs in the soil.展开更多
It has been reported that there is an interaction between Benzo[a]pyrene (BaP), a widespread carcinogenic polycyclic aromatic hydrocarbon, and tributyltin (TBT), an organometal used as an antifouling biocide. This...It has been reported that there is an interaction between Benzo[a]pyrene (BaP), a widespread carcinogenic polycyclic aromatic hydrocarbon, and tributyltin (TBT), an organometal used as an antifouling biocide. This study was therefore designed to examine the potential in vivo influence of BaP, TBT and their mixture on splenic antioxidant defense systems of Sebastiscus marmoratus. The fish were exposed to water containing environmentally relevant concentrations of BaP, TBT and their mixture. Spleens were collected for biochemical analysis after exposure for 7, 25, 50 d and after recovery for 7, 20 d. Cotreatment with BaP and TBT for 7 d potentiated the induction of glutathione peroxidase (GPx) activity by BaP or TBT alone. The cotreatment for 25 and 50 d resulted in inhibition of GPx activity, which was similar to the effect of TBT. Splenic glutathione S-transferase (GST) activities were significantly elevated in S. marmoratus exposed to BaP starting from 7 d and remained high up to 25 d. However, no further activity change was found with prolonged exposure. Cotreatment of BaP and TBT primarily inhibited the GST activity, which was similar to the effect of TBT. Cotreatment with BaP and TBT for 25 or 50 d potentiated the depletion of GSH (glutathione) by BaP or TBT alone. MDA (malondialdehyde) contents in spleen of S. marmoratus were not significantly altered compared with the control during the test period. Spleen, as an immune organ, is sensitive to exposure of BaP or TBT. It should have an effective mechanism to counteract oxidative damage. Antioxidative defense systems in spleen of S. marmoratus should be considered as potential biomarkers. Short-term exposure of BaP or TBT could result in induction of antioxidant defense system. A significant decrease of these indices, such as GSH, GST, GPx might indicate more severe contamination.展开更多
Seedling emergence tests were conducted in a meadow brown soil using five plant species(i.e., Chinese cabbage, green onion, tomato, turnip and wheat) to determine the phytotoxicity of phenanthrene, pyrene and their mi...Seedling emergence tests were conducted in a meadow brown soil using five plant species(i.e., Chinese cabbage, green onion, tomato, turnip and wheat) to determine the phytotoxicity of phenanthrene, pyrene and their mixtures. The soil was amended with up to 1000 mg/kg soil of phenanthrene or 600 mg/kg soil of pyrene. Seedling emergence and root growth were measured. The results indicated that root elongation was more sensitive than seedling emergence. Root length decreased with increasing phenanthrene or pyrene concentrations(p≤0 05). Phenanthrene was more phytotoxic than pyrene. The lowest observable adverse effect concentration(LOAEC) was 10 mg/kg for phenanthrene when tested with green onion, and 50 mg/kg for pyrene when tested with wheat. Among the five species, wheat was found to be the most sensitive. When amended jointly at or below their respective LOAEC, phenanthrene and pyrene produced a synergistic toxic effect.展开更多
Objective To study the combined effect of fluoride and arsenate on the expression of SD rat osteoblastic osteoclast differentiation factor (ODF) mRNA and osteoprotegerin (OPG) mRNA. Methods Osteoblasts were obtain...Objective To study the combined effect of fluoride and arsenate on the expression of SD rat osteoblastic osteoclast differentiation factor (ODF) mRNA and osteoprotegerin (OPG) mRNA. Methods Osteoblasts were obtained by enzymatic isolation from newborn SD rats. A factorial experiment was performed. Osteoblasts were exposed to NaF (0.5 mmolF/L, 4 molF/L) and Na3AsH2 (12.5 μmolAs/L and 200 μmolAs/L) separately or F plus As and cultured for 48 h. The gene expression of osteoblastic ODF and OPG was observed by RT-PCR. Results The expression ofODF mRNA increased in F0.5, F4 groups compared with control group and two groups of F0.As200, F,As200 compared with As200 group, and decreased significantly in groups of F4As12.5, F0.5As200, and F4As200. The expression of OPG rnRNA decreased in groups of F4, As200, F4As12.5, F0.5As200, and F4As200. Conclusion The joint effect of fluoride and arsenate on the gene expression of ODF is antagonistic, while the combined effect on the gene expression of OPG is synergistic. F4, F4As12.5, and F0.5As200 promote bone resorption of rat osteoclasts, whereas F0.5As12.5 inhibits osteolytic effect of rat osteoclasts.展开更多
Objective Evidence is lacking regarding the combined effects of smoking and obesity on mortality from coronary heart disease in male veterans.This study aimed to explore the combined effect of smoking and obesity on c...Objective Evidence is lacking regarding the combined effects of smoking and obesity on mortality from coronary heart disease in male veterans.This study aimed to explore the combined effect of smoking and obesity on coronary heart disease mortality in male veterans in China.Methods A cohort of 1,268 male veterans from 22 veteran centers in Xi’an(Shaanxi Province,China)were followed up once every 2 years from February 1,1987 to October 30,2016.The endpoint was death from any cause.The hazard ratio(HR)of each risk factor and the 95%confidence interval(CI)were calculated using a multivariate Cox proportional hazard model.Results The total follow-up was 24394.21 person-years;each subject was followed up for a mean duration of 19.24 years.By the end of the study,of the 1,268 veterans,889 had died,363 were alive,and 16 were lost to follow-up.Cox regression analysis results revealed that current smoking(HR:1.552,95%CI:1.074–2.243),obesity(HR:1.625,95%CI:1.024–2.581),and the combined effect of the two factors(HR:2.828,95%CI:1.520–5.262)were associated with coronary heart disease mortality.Conclusion Our results suggest that obese veterans who smoke might be an important target population for coronary heart disease mortality control.展开更多
Developing single-atom catalysts(SACs) for electrochemical devices is a frontier in energy conversion.The comparison of stability,activity and selectivity between various single atoms is one of the main research focus...Developing single-atom catalysts(SACs) for electrochemical devices is a frontier in energy conversion.The comparison of stability,activity and selectivity between various single atoms is one of the main research focuses in SACs.However,the in-depth understanding of the role that the coordination atoms of single atom play in the catalytic process is lacking.Herein,we proposed a graphene-like boroncarbon-nitride(BCN) monolayer as the support of single metal atom.The electrocatalytic nitrogen reduction reaction(eNRR) performances of 3 d,4 d transition metal(TM) atoms embedded in defective BCN were systematically investigated by means of density functional theory(DFT) computations.Our study shows that the TM-to-N and B-to-N π-back bonding can contribute to the activation of N_(2).Importantly,a combined effect is revealed between single TM atom and boron atom on eNRR:TM atom enhances the nitrogen reduction process especially in facilitating the N_(2) adsorption and the NH3 desorption,while boron atom modulates the bonding strength of key intermediates by balancing the charged species.Furthermore,Nb@BN3 possesses the highest electrocata lytic activity with limiting potential of-0.49 V,and exhibits a high selectivity for nitrogen reduction reaction(NRR) to ammonia compared with hydrogen evolution reaction(HER).As such,this work can stimulate a research doorway for designing multi-active sites of the anchored single atoms and the innate atoms of substrate based on the mechanistic insights to guide future eNRR research.展开更多
Microplastics pollution and seawater temperature rise have been the major environmental issues,threatening the survival and biodiversity of marine organisms.This study evaluated the combined effect of temperature and ...Microplastics pollution and seawater temperature rise have been the major environmental issues,threatening the survival and biodiversity of marine organisms.This study evaluated the combined effect of temperature and polystyrene microplastics(MP)on Artemia,a filter-feeding crustacean that is widely used for environmental toxicology studies.Brine shrimp Artemia franciscana were exposed to three MP concentrations(0,0.2,and 2.0 mg/L)and three temperatures(22,26,and 30℃)for 14 d.In general,higher MP concentration and temperature led to a decreased survival rate and growth.Two-way ANOVA analysis indicated that the survival rate of Artemia was significantly impacted by both MP concentration and temperature(P<0.05),but there was no significant interaction between two factors(P>0.05).Growth of Artemia was significantly impacted by temperature(P<0.05),and with a significant interaction between two factors(P<0.05).Furthermore,the enzymatic activity,intestinal histological analyses,and immune gene expression were determined for Artemia reared at 30℃ with three MP concentrations(0,0.2,and 2.0 mg/L).The results showed that 2.0-mg/L MP resulted in reduced Artemia intestinal microvilli and exfoliated epithelia cells,significantly increased acid phosphatase(ACP)activity(P<0.05)and immunerelated gene ADRA1 B and CREB3 expression,revealing that higher MP concentration could induce oxidative and immunological stress on Artemia at 30℃.Overall,our study suggests that MP and temperature have combined adverse effect on Artemia,especially at relatively high temperature and polystyrene MP concentration.These findings are important to understand the potential ecological risks posed by these two factors on the organisms in marine environment.展开更多
Dyes in wastewater are of particular environmental concern since they give an undesirable color to the waters, dye and their derivatives are harmful to human health and the environment ( Annadurai et al., 2002). Due...Dyes in wastewater are of particular environmental concern since they give an undesirable color to the waters, dye and their derivatives are harmful to human health and the environment ( Annadurai et al., 2002). Due to their biological persistency, conventional biological treat-ment processes such as activated sludge process are ineffective for their removal(Shaul et al., 1991). There is a need to develop effective methods for the degradation of such persistent organic pollutants, either to less harmful compounds or, more desirable, to their complete mineralization.展开更多
Passivating undercoordinated ions is an effective way to reduce the defect densities at the surface and grain boundaries(GBs)of perovskite materials for enhanced photovoltaic performance and stability of perovskite so...Passivating undercoordinated ions is an effective way to reduce the defect densities at the surface and grain boundaries(GBs)of perovskite materials for enhanced photovoltaic performance and stability of perovskite solar cells(PSCs).Here,(BBF)complex is chosen as a multifunctional additive,which contains both C7H9N and BF3 groups working as Lewis base and Lewis acid,respectively,can bond with Pb^(2+)/I^(−) and FA+on the surface and in the GBs in the perovskite film,affording passivation of both cation and anion defects.The synergistic effect of the C7H9N and BF3 complex slows the crystallization during the perovskite film deposition to improve the crystalline quality,which reduces the trap density and the recombination in the perovskite film to suppress nonradiative recombination loss and minimizes moisture permeation to improve the stability of the perovskite material.Meanwhile,such an additive improves the energy-level alignment between the valence band of the perovskite and the highest occupied molecular orbital of the hole-transporting material,Spiro-OMeTAD.Consequently,our work achieves power conversion efficiency of 23.24%,accompanied by enhanced stability under ambient conditions and light illumination and opens a new avenue for improving the performance of PSCs through the use of a multifunctional complex.展开更多
基金The National Key Research and Development Program of China under contract No.2022YFE0140500the National Natural Science Foundation of China under contract Nos 41821004 and 42130406+2 种基金the National Natural Science Foundation of China-Shandong Joint Fund under contract No.U1906215the Open Fund of Key Laboratory of Ocean Circulation and Waves,Chinese Academy of Sciences under contract No.KLOCW2003the Project of Doctoral Found of Qingdao University of Science and Technology under contract No.210010022746.
文摘As important atmospheric circulation patterns in Northern Hemisphere(NH),the North Atlantic Oscillation(NAO)and the Western Pacific teleconnection(WP)affect the winter climate in Eurasia.In order to explore the combined effects of NAO and WP on East Asian(EA)temperature,the NAO and WP indices are divided into four phases from 1980−2021:the positive NAO and WP phase(NAO+/WP+),the negative NAO and WP phase(NAO−/WP−),the positive NAO and negative WP phase(NAO+/WP−),the negative NAO and positive WP phase(NAO−/WP+).In the phase of NAO+/WP+,the low geopotential height(GH)stays in north of EA at 50°−80°N;the surface air temperature anomaly(SATA)is 0.8−1℃lower than Southern Asian.In the phase of NAO−/WP−,the center of high temperature and GH locate in the northeast of EA;the cold air spreads to Southern Asia,causing the SATA decreases 1−1.5℃.In the phase of NAO+/WP−,the high GH belt is formed at 55°−80°N.Meanwhile,the center of high SATA locates in the north of Asia that increases 0.8−1.1℃.The cold airflow causes temperature dropping 0.5−1℃in the south of EA.The SATA improves 0.5−1.5℃in south of EA in the phase of NAO−/WP+.The belt of high GH is formed at 25°−50°N,and blocks the cold air which from Siberia.The NAO and WP generate two warped plate pressure structures in NH,and affect the temperature by different pressure configurations.NAO and WP form different GH,and GH acts to block and push airflow by affecting the air pressure,then causes the temperature to be different from the north and south of EA.Finally,the multiple linear regression result shows that NAO and WP are weakened by each other such as the phase of NAO+/WP+and NAO−/WP−.
文摘In this study,we have examined the effects of exposure to high temperature, carbon inonoxideor a combination of both conditions in a model system,the rat and in industrial workers.In the rat liver, HSP70 mRNA and HSP70 synthesis were measured by dot hybridization and western blot. The results showed that after a heat stress HSP70 mRNA and its product, HSP70 increased significantly and there was a synergism in the combined effects of high temperature and carbon monoxide exposure on the induction of HSP70 mRNA and HSP70 synthesis. Heat played a major role in this induction. The presence of antibodies to human HSP27, HSP60, HSP70,HSC73, HSP89 αand β in workers exposed to heat, carbon monoxide was also measured by western blot using purified HSPs as antigens. Plasma free amino acids were measured in the saine group of workers. The incidence of antibodies to HSP27 and HSP70 Was significantly higher in the workers working in an environment with extreme heat, and high carbon monoxide ernission than in a control group. The carbon monoxide exposed group showed the highest incidence of antibodies to HSPs. Although our previous results indicated that workers had an insufficient protein intake,plasma free amino acids tended to increase, especially in methionine and tryptophan two kinds of amino acids which are absent from the main stress protein, HSP70.These results suggest that the major problems that these workers may face are how to facilitate the use of plasma free amino acids and reduce the inhibition of synthesis of normal proteins when they are exposed to occupational harmful factors.These resultsalso add new information on the measurement of HSPs as a potential biomonitor to assess whether organisms are experiencing metabolic stress within their environment.
基金Funded by the National Natural Science Foundation of China(Nos.51279017,51539002 and 51139001)China Postdoctoral Science Foundation Funded Project(No.2015M582213)
文摘The hydraulic concrete durability under the alternation of freeze-thaw and carbonation has been systematically investigated in this work, where both the micro part and the microscopic characteristics of concrete interface were analyzed based on computed tomography(CT) test and scanning electron microscopy(SEM). Average CT numbers of each section, declined at water-cement ratio of 0.35, increased at 0.45, and changed a little at 0.55. The specimen in the absence of fly ash exhibited less types of hydration products and the surface was observed to be a needle-like ettringite, with a relatively dense overall structure. However, with the increase of fly ash content, pores and micro-cracks of specimen structure increased. Hexagonal flake calcium hydroxide, present in the specimen after the first carbonation, was negligible in the test pieces of the first freezethaw where the main hydration products were ettringite and calcium silicate gel. Regular hexagonal plates of calcium hydroxide exhibited in the interior of the specimen in which charring first occurred but calcium hydroxide rarely existed in the interior of the specimen in which freeze-thaw first occurred.
基金The Guangdong Province Education Department under contract No.GCZX-A0909the Guangdong Province Ocean and Fisheries Science & Technology Extension Project under contract No.20120980+1 种基金the Guangdong Province Industry-University-Science Partnership Project under contract No.20110908the Science&Technology Project of Huaiyin Normal University under contract No.WH0031
文摘Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18–34°C) and copper ion concentration(0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P〈0.01), the quadratic effect of temperature was significant(P〈0.05); the linear effect of copper ion concentration was not significant(P〉0.05), while the quadratic effect of copper ion concentration was highly significant(P〈0.01); the interactive effect of temperature and copper ion concentration was not significant(P〉0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.
文摘The influences of the calmodulin antagonist chlorpromazine (CPZ), and calcium channel blocker nimodipine (NIMO) and their combination on cadmium (Cd) poisoning of mice were studied. A seties of biochemical parameters including urinary enzyme activities, blood and urine Cd levels, metallothionein (MT) contents in liver and kidney, hepatic ultrastructure and Ca2+ -Mg2+ AT-Pase activity in erythrocyte membrane were determined. Animal models for Cd poisoning were established by peritoneal injection of 1/5 LD50 CdCl2. The experimental groups were protected by administration of CPZ, NIMO and CPZ and NIMO in combination l h before the injection of CdCl2. Five days later, samples were collected for analysis. The data showed that Crs could protect kidney tissue against Cd-induced damage, as the urinary γ-glutamyl traspepti dase (γ- GT ) and N- acetyl-β-D-glucosaminidase (NAG) activities were reduced significantly. There was neither evidence of the protective effect of NIMO on kidney tissue nor an indication of a synergistic effecf of Crs and NIMO.Both CPZ and NIMO showed a considerable protective effect against the deerease in Ca2+ -Mg2+ AT-Pase activity, and a synergistic action was observed. Cd content in blood was reduced significanily by CPZ or the combination of CPZ and NIMO, but elevated by NIMO. Both CPZ and NIMO consideraby increased MT contents in livers and kidneys and ameliorated damaged to the hepatic ultrastructures caused by Cd. The results indicated that these inhibitors could protect mice against the toxic effects of Cd in liver and kidney tissues, while CPZ was more efficient than NIMO. The combination of CPZ and NIMO exerted a synergistic action. The protective action of these two drugs might be relevent to the function of MT.
基金supported by the Department of Science and Technology of Jilin Province.[Grant No:20210204024YY]。
文摘Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B(UVB)irradiation remain unclear.This study was undertaken to explore the effects of combination of 1,25(OH)_(2)D_(3) and TAK-242(TLR-4 inhibitor)on the damage to HaCaT cells caused by UVB irradiation.Methods In vitro,HaCaT cells were treated with 1,25(OH)_(2)D_(3) or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm^(2),then the production of reactive oxygen species(ROS),cell migration,apoptosis of cells,and the expression of oxidative stress,endoplasmic reticulum stress,and apoptosis related proteins were determined.Results Compared with the HaCaT cells treated with 1,25(OH)_(2)D_(3) or TAK-242,the cells treated with both 1,25(OH)_(2)D_(3) and TAK-242 showed,1)significantly lower production of ROS(P<0.05);2)significantly less apoptosis of HaCaT cells(P<0.05);3)significantly lower expression of NF-κB,Caspase-8,Cyto-C,Caspase-3(P<0.05).Conclusion The combination of 1,25(OH)_(2)D_(3) and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress,endoplasmic reticulum stress and apoptosis than 1,25(OH)_(2)D_(3) or TAK-242 alone.
文摘BACKGROUND: Central adrenergic nerve and 5-serotonergic nerve can influence central cholinergic nerve on learning and memory and make easy for study; however, ginsenoside of stem and leaf (GSL) can improve functions of central adrenergic nerve; moreover, 5-serotonergic nerve and the combination with choline can produce synergistic effect and enhance learning and memory ability so as to improve learning and memory disorder of patients with Alzheimer disease (AD). OBJECTIVE : To observe the effects of GSL combining with choline on learning and memory of AD model rats DESIGN : Randomized grouping design and controlled animal study SETIING : Department of Pharmacology, Taishan Medical College MATERIALS : The experiment was carried out in the Pharmacological Department of Medical College of Jilin University from October 1996 to January 1997. Forty healthy male Wistar rats of clean grade were randomly divided into 5 groups, including sham-injury group, model group, GSL group, choline group and combination group, with 8 rats in each group. Main medications: GSL with the volume more than 92.8% was provided by Department of Chemistry, Norman Bethune Medical College of Jilin University. Panaxatriol, the main component, was detected with thin layer scanning technique and regarded as the index of GSL quality [(55±1)%, CV= 2%, n = 5]. Choline was provided by the Third Shanghai Laboratory Factory. METHODS : 150 nmol quinolinic acid was used to damage bilateral Meynert basal nuclei of adult rats so as to establish AD models. Rats in GSL, choline and combination groups were intragastric administrated with 400 mg/kg GSL, 200 mg/kg choline (20 mL/kg), and both respectively last for 17 days starting from two days before operation. Rats in sham-injury group and model group were perfused with the same volume of distilled water once in each morning for the same days. (1) Passive avoidance step-down test: Five minutes later, rats jumped up safe platform when they were shocked with 36 V alternating current. If rats jumped down from the platform and the feet touched railings, the response was wrong. Numbers of wrong response were recorded within 3 minutes, and then the test was redone after 24 hours. (2) Morris water-maze spatial localization task: Swimming from jumping-off to platform directly was regarded as right response. Additionally, 4 successively right responses were regarded as the standard. Each rat was trained 10 times a day with 120 s per time for 3 successive days. The interval was 30 s. Three days later, numbers of right response were recorded. The training times were increased to 30 for unlearned rats. (3) Measurement of activity of choline acetylase in cerebral cortex: Rats were sacrificed at 17 days after operation to obtain cerebral cortex to measure activity of choline acetylase with radiochemistry technique. (4) Synergistic effect: It was expressed as Q value: Q value = factual incorporative effect/anticipant incorporative effect; Q ≥ 1 was regarded as synergistic effect. Anticipant incorporative effect = (EA+EB-EA·EB), EA and EB were single timing effect, respectively in GSL group and choline group. E(step-down test and Morris water maze test) = (x in model group - factual value in medicine groups)/x in model group; E (activity of choline acetylase) = (factual value in medicine groups -xin model group)/xin model group. MAIN OUTCOME MEASURES : (1) Passive avoidance step-down test and Morris water-maze spatial localization task in the study of learning and memory; (2) activity of choline acetylase. RESULTS : All 40 rats were involved in the final analysis. (1) Passive avoidance response: At learning phase on first day and retesting phase on the next day, numbers of wrong responses within 3 minutes were more in model group than sham operation group, and there was significant difference [(5.88±1.46), (2.25±0.87) times; (2.63±1.06), (0.50±0.53) times; P 〈 0.01]; numbers of wrong responses within 3 minutes were less in combination group than model group, and there was significant difference [learning phase: (1.12±0.83), (5.88±1.46) times; retesting phase: (0.38±0.74), (2.63±1.06)times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and 1.59, respectively and it showed synergistic effect. Spatial localization task: Training times were more in model group than sham operation group, and there was significant difference [(2.9±2.5), (12.6±3.5) times; P 〈 0.01]. Training times were less in combination group than model group, and there was significant difference [(11.8±2.4), (27.9±2.5) times, P 〈 0.01]; moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.07 and it showed synergistic effect. (3) Activity of choline acetylase: Activity was lower in model group than sham operation group, and there was significant difference [(30.56±8.33), (61.11 ±8.33) nkat/g; P 〈 0.01]. Activity was higher in combination group than model group and there was significant difference [(50.00±8.33), (30.56±8.33) nkat/g, P 〈 0.01];moreover, effect was stronger than that in GSL group and choline group. The Q value was 1.5 and it showed synergistic effect. CONCLUSZON: GSL in combination with choline can synergically improve the disorder of learning and memory of AD model rats. Its mechanism may be involved in enhancing the function of central cholinergic system.
文摘The effect of the Chinese herbal compound (CHC) on solid sarcoma 180 (S180) in Swiss mice was studied either alone or in combination with 5-fluorouracil (5FU), cyclophosphamide (CYT) or mitomycin C (MMC). The preliminary results indicated that combination treatment seemed to possess better antitumor activity than chemotherapy alone. The treatment with CHC alone however had neither an obvious antitumor effect in tumor bearing mice nor toxicity in normal mice. These results show that CHC may stimulate organs of the immune system such as the spleen to be im-munomodulators and enhance the antitumor activity of some chemotherapeutic agents.
文摘The effects of bleomycin A5 (BLM A5) alone and combined with calmodulin inhibitor N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide (W-13) on the proliferation on S-180 cells in vitro were studied. IC50 of BLM used alone for the cells was about 2.63 μg/ml, but it was reduced to 1/3.8 and 1/9.5 of 2.63 μg/ml when plus W-13 1, 5 μg/ml respectively. The results indicated that nontoxic doses of W-13 enhanced the hinibition of cell proliferation under the condition of BLM 0.5 - 2.5 μg/ ml. In colony forming test, the survival fraction of S-180 cells treated with BLM plus W-13 was decreased to 1/87 - 240 of that of the cells treated with BLM alone. The results suggest that W-13 can enhance antitumor activity of BLM in vitro and may be used as an synergist of BLM A5 in vivo.
文摘To investigate the effects of patient-controlled intravenous analgesia (PCIA) with lornoxicam and fentanyl on arrhythmia and the expression of platelet membrane glycoproteins in patients with coronary artery disease (CAD) after abdominal surgery.Methods Eighty ASA Ⅱ or Ⅲ patients with CAD aged 51~66 yrs weighing 59~68 kg presenting for abdominal surgery participated in this study.CAD was diagnosed by clinical symptoms and ischemic changes on ECG.The patients were premedicated with intramuscular henobarbital 0.1 g and scopolamine 0.3 mg.Anesthesia was induced with fentanyl,droperidol,propofol and vecuronium and maintained with propofol,fentanyl and vecuronium.The patients received PCIA after operation.The PCIA solution contained fentanyl 0.9 mg and droperidol 5 mg in 100 ml of normal saline (N.S.) in group A (n=40) or lornoxicam 56 mg,fentanyl 0.2 mg and droperidol 5 mg in 100 ml N.S. in group B (n=40).In group A the loading dose was fentanyl 0.05 mg and group B lornoxicam 4 mg.PCIA included a background infusion at 2 ml·h -1 and a bolus of 0.5 ml with a 15 min lock-out.VAS(0=no pain,10= worst pain) was used to measure pain intensity.In addition to BP,HR and SpO2 monitoring ECG was continuously monitored with a Holter monitor after operation.Blood samples were taken from peripheral vein before and 6 h after operation and on the 1st,2nd,7th and 8th postoperative days for determination of the expression of CD 62p ,CD 63 and CD 41 /CD 61 on the platelet membrane,platelet count,prothrombin time (PT) thrombin time (TT) and partial thromboplastin time (PTT).Results The two groups were comparable with respect to sex,age,body weight,severity of CAD,duration of operation and intraoperative blood loss.The patients received no blood transfusion during operation.There was no significant difference in VAS score,platelet count,PT,TT and PTT between the two groups.The incidence of atrial and ventricular premature beat on ECG and the expression of CD 41 /CD 61 ,CD 62p and CD 63 on the platelet membrane were significantly lower in group B than in group A on the 7th and 8th postoperative days(P<0.05 or 0.01).Conclusion Postoperative PCIA with lornoxicam and fentanyl can more effectively reduce the incidence of postoperative arrhythmia in patients with CAD.Suppression of activation of platelets by lornoxicam may contribute to the mechanism.10 refs,3 tabs.
基金financially supported by the Fund for the Shenzhen Science and Technology Innovation Commission(Nos.WDZC20200819163549002 and KCXFZ20211020163556020).
文摘It has been confirmed that microplastics(MPs)are present in the environment.This study simulated secondary PE-MPs via aging and mechanical processes to evaluate their effects on Pak choi(Brassica rapa L.)over 21 d.Two common pollutants,dichlorodiphenyltrichloroethane(DDT)and naphthalene,were used in the combined toxicity tests.The results indicated that the growth of Pak choi was significantly inhibited after exposure to secondary PE-MPs,and the combined effects were antagonistic,owing to the adsorption capacity of secondary PE-MPs to DDT and naphthalene.Oxidative stress in Pak choi can be markedly affected,leading to oxidative damage to plant cells.The moisture content,soil bulk density,soil density,cation exchange capacity(CEC),and FDA hydrolase in the planted soils increased in the treated groups,and the TOC content changed significantly.We also found that the microbial composition of the soil in the DDT and naphthalene groups showed more significant alterations than that in the other groups.Alpha diversity analysis showed that species diversity increased in the combined groups but indicated a clear downward trend in the single MPs groups.This study suggests that secondary PE-MPs harm the growth of Pak choi and can change soil properties,revealing the harm to the ecosystem of MPs in the soil.
基金Project supported by the National Natural Science Foundation of China(No.20447001, 40476049)the Natural Science Foundation of Fujian Province of China(No. D0410007).
文摘It has been reported that there is an interaction between Benzo[a]pyrene (BaP), a widespread carcinogenic polycyclic aromatic hydrocarbon, and tributyltin (TBT), an organometal used as an antifouling biocide. This study was therefore designed to examine the potential in vivo influence of BaP, TBT and their mixture on splenic antioxidant defense systems of Sebastiscus marmoratus. The fish were exposed to water containing environmentally relevant concentrations of BaP, TBT and their mixture. Spleens were collected for biochemical analysis after exposure for 7, 25, 50 d and after recovery for 7, 20 d. Cotreatment with BaP and TBT for 7 d potentiated the induction of glutathione peroxidase (GPx) activity by BaP or TBT alone. The cotreatment for 25 and 50 d resulted in inhibition of GPx activity, which was similar to the effect of TBT. Splenic glutathione S-transferase (GST) activities were significantly elevated in S. marmoratus exposed to BaP starting from 7 d and remained high up to 25 d. However, no further activity change was found with prolonged exposure. Cotreatment of BaP and TBT primarily inhibited the GST activity, which was similar to the effect of TBT. Cotreatment with BaP and TBT for 25 or 50 d potentiated the depletion of GSH (glutathione) by BaP or TBT alone. MDA (malondialdehyde) contents in spleen of S. marmoratus were not significantly altered compared with the control during the test period. Spleen, as an immune organ, is sensitive to exposure of BaP or TBT. It should have an effective mechanism to counteract oxidative damage. Antioxidative defense systems in spleen of S. marmoratus should be considered as potential biomarkers. Short-term exposure of BaP or TBT could result in induction of antioxidant defense system. A significant decrease of these indices, such as GSH, GST, GPx might indicate more severe contamination.
文摘Seedling emergence tests were conducted in a meadow brown soil using five plant species(i.e., Chinese cabbage, green onion, tomato, turnip and wheat) to determine the phytotoxicity of phenanthrene, pyrene and their mixtures. The soil was amended with up to 1000 mg/kg soil of phenanthrene or 600 mg/kg soil of pyrene. Seedling emergence and root growth were measured. The results indicated that root elongation was more sensitive than seedling emergence. Root length decreased with increasing phenanthrene or pyrene concentrations(p≤0 05). Phenanthrene was more phytotoxic than pyrene. The lowest observable adverse effect concentration(LOAEC) was 10 mg/kg for phenanthrene when tested with green onion, and 50 mg/kg for pyrene when tested with wheat. Among the five species, wheat was found to be the most sensitive. When amended jointly at or below their respective LOAEC, phenanthrene and pyrene produced a synergistic toxic effect.
文摘Objective To study the combined effect of fluoride and arsenate on the expression of SD rat osteoblastic osteoclast differentiation factor (ODF) mRNA and osteoprotegerin (OPG) mRNA. Methods Osteoblasts were obtained by enzymatic isolation from newborn SD rats. A factorial experiment was performed. Osteoblasts were exposed to NaF (0.5 mmolF/L, 4 molF/L) and Na3AsH2 (12.5 μmolAs/L and 200 μmolAs/L) separately or F plus As and cultured for 48 h. The gene expression of osteoblastic ODF and OPG was observed by RT-PCR. Results The expression ofODF mRNA increased in F0.5, F4 groups compared with control group and two groups of F0.As200, F,As200 compared with As200 group, and decreased significantly in groups of F4As12.5, F0.5As200, and F4As200. The expression of OPG rnRNA decreased in groups of F4, As200, F4As12.5, F0.5As200, and F4As200. Conclusion The joint effect of fluoride and arsenate on the gene expression of ODF is antagonistic, while the combined effect on the gene expression of OPG is synergistic. F4, F4As12.5, and F0.5As200 promote bone resorption of rat osteoclasts, whereas F0.5As12.5 inhibits osteolytic effect of rat osteoclasts.
基金supported by the Fund of the Military Medical Scientific Research[20BJZ46]the Special Project of Health Care from the Central Committee of Healthcare[W2013BJ32]。
文摘Objective Evidence is lacking regarding the combined effects of smoking and obesity on mortality from coronary heart disease in male veterans.This study aimed to explore the combined effect of smoking and obesity on coronary heart disease mortality in male veterans in China.Methods A cohort of 1,268 male veterans from 22 veteran centers in Xi’an(Shaanxi Province,China)were followed up once every 2 years from February 1,1987 to October 30,2016.The endpoint was death from any cause.The hazard ratio(HR)of each risk factor and the 95%confidence interval(CI)were calculated using a multivariate Cox proportional hazard model.Results The total follow-up was 24394.21 person-years;each subject was followed up for a mean duration of 19.24 years.By the end of the study,of the 1,268 veterans,889 had died,363 were alive,and 16 were lost to follow-up.Cox regression analysis results revealed that current smoking(HR:1.552,95%CI:1.074–2.243),obesity(HR:1.625,95%CI:1.024–2.581),and the combined effect of the two factors(HR:2.828,95%CI:1.520–5.262)were associated with coronary heart disease mortality.Conclusion Our results suggest that obese veterans who smoke might be an important target population for coronary heart disease mortality control.
基金the Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China(grant number 161008)the Basic Research Program of Shenzhen(grant number JCYJ20190809120015163)+4 种基金the Key R&D Program of Hubei province(grant number 2020CFA087)the Fundamental Research Funds for the Central Universities(grant number 2019III-034)the Xiamen University Malaysia Research Fund(grant number XMUMRF/2019-C3/IENG/0013)the Ministry of Higher Education(MOHE)Malaysia under the Fundamental Research Grant Scheme(FRGS)(grant number FRGS/1/2020/TK02/XMU/02/1)the Overseas Expertise Introduction Project(111 project)for Discipline Innovation of China(grant number B18038)。
文摘Developing single-atom catalysts(SACs) for electrochemical devices is a frontier in energy conversion.The comparison of stability,activity and selectivity between various single atoms is one of the main research focuses in SACs.However,the in-depth understanding of the role that the coordination atoms of single atom play in the catalytic process is lacking.Herein,we proposed a graphene-like boroncarbon-nitride(BCN) monolayer as the support of single metal atom.The electrocatalytic nitrogen reduction reaction(eNRR) performances of 3 d,4 d transition metal(TM) atoms embedded in defective BCN were systematically investigated by means of density functional theory(DFT) computations.Our study shows that the TM-to-N and B-to-N π-back bonding can contribute to the activation of N_(2).Importantly,a combined effect is revealed between single TM atom and boron atom on eNRR:TM atom enhances the nitrogen reduction process especially in facilitating the N_(2) adsorption and the NH3 desorption,while boron atom modulates the bonding strength of key intermediates by balancing the charged species.Furthermore,Nb@BN3 possesses the highest electrocata lytic activity with limiting potential of-0.49 V,and exhibits a high selectivity for nitrogen reduction reaction(NRR) to ammonia compared with hydrogen evolution reaction(HER).As such,this work can stimulate a research doorway for designing multi-active sites of the anchored single atoms and the innate atoms of substrate based on the mechanistic insights to guide future eNRR research.
基金Supported by the Science and Technology Project of Tianjin Municipal(No.17ZXZYNC00060)the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT_17R81)+1 种基金the National Science Foundation of Tianjin(No.18JCQNJC78500)the Foundation of Tianjin Key Laboratory of Marine Resources and Chemistry(Tianjin University of Science&Technology),China(No.201704)。
文摘Microplastics pollution and seawater temperature rise have been the major environmental issues,threatening the survival and biodiversity of marine organisms.This study evaluated the combined effect of temperature and polystyrene microplastics(MP)on Artemia,a filter-feeding crustacean that is widely used for environmental toxicology studies.Brine shrimp Artemia franciscana were exposed to three MP concentrations(0,0.2,and 2.0 mg/L)and three temperatures(22,26,and 30℃)for 14 d.In general,higher MP concentration and temperature led to a decreased survival rate and growth.Two-way ANOVA analysis indicated that the survival rate of Artemia was significantly impacted by both MP concentration and temperature(P<0.05),but there was no significant interaction between two factors(P>0.05).Growth of Artemia was significantly impacted by temperature(P<0.05),and with a significant interaction between two factors(P<0.05).Furthermore,the enzymatic activity,intestinal histological analyses,and immune gene expression were determined for Artemia reared at 30℃ with three MP concentrations(0,0.2,and 2.0 mg/L).The results showed that 2.0-mg/L MP resulted in reduced Artemia intestinal microvilli and exfoliated epithelia cells,significantly increased acid phosphatase(ACP)activity(P<0.05)and immunerelated gene ADRA1 B and CREB3 expression,revealing that higher MP concentration could induce oxidative and immunological stress on Artemia at 30℃.Overall,our study suggests that MP and temperature have combined adverse effect on Artemia,especially at relatively high temperature and polystyrene MP concentration.These findings are important to understand the potential ecological risks posed by these two factors on the organisms in marine environment.
文摘Dyes in wastewater are of particular environmental concern since they give an undesirable color to the waters, dye and their derivatives are harmful to human health and the environment ( Annadurai et al., 2002). Due to their biological persistency, conventional biological treat-ment processes such as activated sludge process are ineffective for their removal(Shaul et al., 1991). There is a need to develop effective methods for the degradation of such persistent organic pollutants, either to less harmful compounds or, more desirable, to their complete mineralization.
基金The authors acknowledge support from the National Natural Science Foundation of China(62074095/61704101/91733301)the National Key Research and Development Program of China(2016YFA0202403)+3 种基金the Fundamental Research Funds for the Central Universities GK(201903048)the 111 Project(Grant No.B21005)the Fundament Research Funds for the Central University(2019TS004)DNL Cooperation Fund CAS(DNL180311).
文摘Passivating undercoordinated ions is an effective way to reduce the defect densities at the surface and grain boundaries(GBs)of perovskite materials for enhanced photovoltaic performance and stability of perovskite solar cells(PSCs).Here,(BBF)complex is chosen as a multifunctional additive,which contains both C7H9N and BF3 groups working as Lewis base and Lewis acid,respectively,can bond with Pb^(2+)/I^(−) and FA+on the surface and in the GBs in the perovskite film,affording passivation of both cation and anion defects.The synergistic effect of the C7H9N and BF3 complex slows the crystallization during the perovskite film deposition to improve the crystalline quality,which reduces the trap density and the recombination in the perovskite film to suppress nonradiative recombination loss and minimizes moisture permeation to improve the stability of the perovskite material.Meanwhile,such an additive improves the energy-level alignment between the valence band of the perovskite and the highest occupied molecular orbital of the hole-transporting material,Spiro-OMeTAD.Consequently,our work achieves power conversion efficiency of 23.24%,accompanied by enhanced stability under ambient conditions and light illumination and opens a new avenue for improving the performance of PSCs through the use of a multifunctional complex.