The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelo...The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelon, a valued and nutritious fruit, remain largely unknown and their functions have not been characterized. In the present study, 22 ClNF-Y genes in watermelon, 29 CsNF-Y genes in cucumber, and 24CmNF-Y genes in melon were identified based on the whole-genome investigation and their protein properties, gene location, gene structure, motif composition, conserved domain, and evolutionary relationship were investigated. ClNF-YB9 from watermelon and its homologs in cucumber and melon were expressed specifically in seeds. Its expression remained low in the early stages of watermelon seed development,increased at 20 days after pollination(DAP), and peaked at 45–50 DAP. Moreover, the knockout mutant Clnf-yb9 exhibited abnormal leafy cotyledon phenotype, implying its critical role during seed formation.Finally, protein interaction assays showed that ClNF-YB9 interacts with all ClNF-YCs and the ClNF-YB9-YC4 heterodimer was able to recruit a ClNF-YA7 subunit to assemble a complete NF-Y complex, which may function in seed development. This study revealed the structure and evolutionary relationships of the NF-Y gene family in Cucurbitaceae and the novel function of ClNF-YB9 in regulating seed development in watermelon.展开更多
[ Objective] This study is to provide basis for the genetic improvement of triploid watermelon cultivars from seed coat characteristiscs by analyzing its genetic properties. [ Method ] Genetic effects of seed coat cha...[ Objective] This study is to provide basis for the genetic improvement of triploid watermelon cultivars from seed coat characteristiscs by analyzing its genetic properties. [ Method ] Genetic effects of seed coat characteristics and their related characteristics in tdploid watermelon were analyzed by additive dominant genetic model based on 36 F1 hybrids which were crossed by six female parents of four ecotypes and six male parents of same ecotype according to North Carolina II crossing design. [ Result] The seed coat number characteristics was controlled by additive effects, dominant effects and error variance of genetic together. The additive effects of the length and width of abortive seed coat were significant, and the error variance was significant or highly significant. It was observed that there was a significant negative correlation of additive effects between the seed coat number and fringe soluble sugar. Also there was a significant negative correlation of dominant effects between lycopenes, dry matter and the seed coat number. However, there was a highly significant positive correlation in additive effects between the length and width of abortive seed coat. [ Conclusion ] The results provide guidance for genetic breeding of triploid watermelon cultivars.展开更多
Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its qu...Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its quality change.In this experiment,seed watermelon kernels were used as research objects,and the effects of 2-Azobis(2-amidinopropane)dihydrochloride(AAPH)on seed watermelon kernel protein isolates(WMP)were investigated.The structure and digestion characteristics of WMP after oxidation were studied.The results showed that with the increase of AAPH concentration(0.05−5 mol/L),WMP showed obvious aggregation,and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL.The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g,α-helix decreased andβ-sheet decreased in secondary structure,and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g,enthalpy(H)and denaturation temperature increased(Td)(P<0.05).By mass spectrometry results of simulated gastric digestion products,it was found that oxidation adversely affected the digestion characteristics of WMP.It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel.展开更多
Response surface methodology was used to optimize the extraction conditions of oil from watermelon seeds. Based on single factor experiments, the material to liquid ratio, extraction time and extraction solvent exhibi...Response surface methodology was used to optimize the extraction conditions of oil from watermelon seeds. Based on single factor experiments, the material to liquid ratio, extraction time and extraction solvent exhibited the highest effects on oil extraction. The mathematical model was established according to the Box-Behnken central composite design. The optimal extraction conditions consisted of a material to liquid ratio of 1：16.3 （g/ml）, an extraction time of 45.4 min, and petroleum ether as the organic solvent. The predicted and actual yields of watermelon seed oil were 27.92% and 28.04%, respectively. The extracted oil was analyzed by GC-MS. The results revealed that watermelon seed oil contained several types of acids and esters ; the relative percentages of the components were determined by a normalization method. The main fatty acids included linoleic acid, palmitic acid, oleic acid, and stearic acid, which accounted for 91.24% of the total components.展开更多
Jingang No. 2 Heimeiren is a new early-maturing watermelon vadety in small to middle size with strong disease resistance bred with ZK09 as female par- ant and ZS05 as male parent. As to whole growth period, the spring...Jingang No. 2 Heimeiren is a new early-maturing watermelon vadety in small to middle size with strong disease resistance bred with ZK09 as female par- ant and ZS05 as male parent. As to whole growth period, the spring crop grows for 90-110 d, the autumn crop grows for 70-80 d, and the fruit development period is 27-29 d. The fruit is oblong with a fruit shape index of 1.5, the average single fruit weight is 3.18 kg, and the fruit surface is smooth in deep green color with dark green netted stripes. The fruit flesh is bright red, crisp, with a soluble solid content in the center of 11.5%. The fruit has a peel thickness of 1.0 cm with high storage tolerance, good appearance and good commodity. The variety is suitable for Guangxi, Guangdong and Halnan.展开更多
基金supported by the National Youth Talent Program (A279021801)Key-Area R&D Program of Guangdong province (2022B0202060001)+4 种基金Key R&D Program of Shaanxi Province (2023-YBNY-008)the Science and Technology Innovation Team of Shaanxi (2021TD-32)the Natural Science Foundation of Shaanxi Province (2021JM-089, 2022JM-112 and 2022JQ-162)the Key R&D Project from Yangling Seed Industry Innovation Center (K3031322016)the Fundamental Research Fund from Northwest A&F University (2452022111)。
文摘The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelon, a valued and nutritious fruit, remain largely unknown and their functions have not been characterized. In the present study, 22 ClNF-Y genes in watermelon, 29 CsNF-Y genes in cucumber, and 24CmNF-Y genes in melon were identified based on the whole-genome investigation and their protein properties, gene location, gene structure, motif composition, conserved domain, and evolutionary relationship were investigated. ClNF-YB9 from watermelon and its homologs in cucumber and melon were expressed specifically in seeds. Its expression remained low in the early stages of watermelon seed development,increased at 20 days after pollination(DAP), and peaked at 45–50 DAP. Moreover, the knockout mutant Clnf-yb9 exhibited abnormal leafy cotyledon phenotype, implying its critical role during seed formation.Finally, protein interaction assays showed that ClNF-YB9 interacts with all ClNF-YCs and the ClNF-YB9-YC4 heterodimer was able to recruit a ClNF-YA7 subunit to assemble a complete NF-Y complex, which may function in seed development. This study revealed the structure and evolutionary relationships of the NF-Y gene family in Cucurbitaceae and the novel function of ClNF-YB9 in regulating seed development in watermelon.
文摘[ Objective] This study is to provide basis for the genetic improvement of triploid watermelon cultivars from seed coat characteristiscs by analyzing its genetic properties. [ Method ] Genetic effects of seed coat characteristics and their related characteristics in tdploid watermelon were analyzed by additive dominant genetic model based on 36 F1 hybrids which were crossed by six female parents of four ecotypes and six male parents of same ecotype according to North Carolina II crossing design. [ Result] The seed coat number characteristics was controlled by additive effects, dominant effects and error variance of genetic together. The additive effects of the length and width of abortive seed coat were significant, and the error variance was significant or highly significant. It was observed that there was a significant negative correlation of additive effects between the seed coat number and fringe soluble sugar. Also there was a significant negative correlation of dominant effects between lycopenes, dry matter and the seed coat number. However, there was a highly significant positive correlation in additive effects between the length and width of abortive seed coat. [ Conclusion ] The results provide guidance for genetic breeding of triploid watermelon cultivars.
基金This study was supported by National Natural Science Foundation of China(No:31760477)Beijing Advanced Innovation Center for Food Nutrition and Human Health(No:20181007)Youth Science and Technology Innovation,Leader in Corps(No:2016BC001).
文摘Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its quality change.In this experiment,seed watermelon kernels were used as research objects,and the effects of 2-Azobis(2-amidinopropane)dihydrochloride(AAPH)on seed watermelon kernel protein isolates(WMP)were investigated.The structure and digestion characteristics of WMP after oxidation were studied.The results showed that with the increase of AAPH concentration(0.05−5 mol/L),WMP showed obvious aggregation,and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL.The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g,α-helix decreased andβ-sheet decreased in secondary structure,and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g,enthalpy(H)and denaturation temperature increased(Td)(P<0.05).By mass spectrometry results of simulated gastric digestion products,it was found that oxidation adversely affected the digestion characteristics of WMP.It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel.
基金Supported by Fund for Independent Innovation of Agricultural Science in Jiangsu Province(CX-125067)
文摘Response surface methodology was used to optimize the extraction conditions of oil from watermelon seeds. Based on single factor experiments, the material to liquid ratio, extraction time and extraction solvent exhibited the highest effects on oil extraction. The mathematical model was established according to the Box-Behnken central composite design. The optimal extraction conditions consisted of a material to liquid ratio of 1：16.3 （g/ml）, an extraction time of 45.4 min, and petroleum ether as the organic solvent. The predicted and actual yields of watermelon seed oil were 27.92% and 28.04%, respectively. The extracted oil was analyzed by GC-MS. The results revealed that watermelon seed oil contained several types of acids and esters ; the relative percentages of the components were determined by a normalization method. The main fatty acids included linoleic acid, palmitic acid, oleic acid, and stearic acid, which accounted for 91.24% of the total components.
文摘Jingang No. 2 Heimeiren is a new early-maturing watermelon vadety in small to middle size with strong disease resistance bred with ZK09 as female par- ant and ZS05 as male parent. As to whole growth period, the spring crop grows for 90-110 d, the autumn crop grows for 70-80 d, and the fruit development period is 27-29 d. The fruit is oblong with a fruit shape index of 1.5, the average single fruit weight is 3.18 kg, and the fruit surface is smooth in deep green color with dark green netted stripes. The fruit flesh is bright red, crisp, with a soluble solid content in the center of 11.5%. The fruit has a peel thickness of 1.0 cm with high storage tolerance, good appearance and good commodity. The variety is suitable for Guangxi, Guangdong and Halnan.
