Protective effect of thodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury

Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons method.After establishing the model,the rats were randomly divided into five groups.Rats in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μL).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm.Rats in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with PBS.Rats in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/mL in a 50-μL cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs.Rats in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside.One week after treatment,exercise recovery was evaluated with a modified combined behavioral score scale.Hematoxylin-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue.Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord.Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord.The results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(P<0.05),the most recovered tissue,and the greatest number of neurons,as indicated by Pischingert’s methylene blue staining.(2)Compared with the PBS group,HIF-1 protein expression was greater in the rhodioloside group(P<0.05).(3)Compared with the Ad-HIF-MSC group,Sry mRNA levels were higher in the rhodioloside+Ad-HIF-MSC group(P<0.05).These results confirm that rhodioloside combined with bone marrow mesenchymal stem cells can promote the recovery of spinal cord injury and activate the HIF-1 pathway to promote the survival of bone marrow mesenchymal stem cells and repair damaged neurons within spinal cord tissue.This experiment was approved by the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine,China(approval No.2015KYLL029)in June 2015.

不同温针灸量联合中药治疗膝骨关节炎临床研究

目的观察不同温针灸量联合中药汤剂治疗膝骨关节炎的疗效并探讨其机制。方法选取109例膝骨关节炎患者,采用随机数字表法分为两组,A组(54例)患者给予温针灸1壮配合温阳通痹汤,B组(55例)患者给予温针灸2壮配合温阳通痹汤。观察比较两组患者治疗后临床疗效,治疗前后炎症因子白细胞介素-1(IL-1)、肿瘤坏死因子α(TNF-α)水平以及治疗前后综合评分、关节炎生活质量测量问卷(AIMS2-SF)、步行距离评分、膝关节屈曲度以及视觉模拟量表(VAS)评分。结果 B组总有效率明显高于A组,两组比较差异具有统计学意义(P<0.05)。B组治疗后炎症因子IL-1、TNF-α均明显低于A组(P<0.05)。两组治疗后综合评分、AIMS2-SF评分均有降低,但B组综合评分、AIMS2-SF评分明显低于A组,差异具有统计学意义(P<0.05)。两组治疗后步行距离评分、膝关节屈曲度均有上升,但B组明显高于A组,差异具有统计学意义(P<0.05)。两组治疗后运动性疼痛评分、压痛点疼痛评分均有降低,但B组患者明显低于A组,差异具有统计学意义(P<0.05)。结论温针灸2壮配合温阳通痹汤治疗膝骨关节炎疗效显著,可有效促进患者康复,缓解疼痛。