[Objective] TO study the combined extracting process of porcine blood superoxide dismutase (SOD) and other bioactive substances and thus to provide technical basis for making full use of blood resources and large-sc...[Objective] TO study the combined extracting process of porcine blood superoxide dismutase (SOD) and other bioactive substances and thus to provide technical basis for making full use of blood resources and large-scale production of SOD. [Method] Fibronectin, immunoglobulin, and hemoglobin were isolated from porcine blood, and SOD was extracted. Trace pyrogallol self-oxidation method to determine SOD activity was modified by optimizing the volume of pyrogallol and SOD samples, reaction temperature, and buffer pH. The specific activity of SOD was determined with the optimized extraction conditions. [ Result] The improved experimental conditions of SOD activity detection were as follows: 7 pyrogallol (50 mmol/L), 3 ml Tris-HCI (50 mmol/L, pH 8.2), reactive temperature at 25(3, and 10 pl SOD sample solution. The specific activity of extracted SOD was 5 056 U/mg protein. [ Conclusion] Four kinds of bioactive substance can be isolated from porcine blood by modern biological engi- neering integration technology, and the extracted SOD has better activity.展开更多
This study aims to investigate the protective effects of BlingLife®-berry extract on the eyes.BlingLife®-berry extract is a mixture of high-quality natural berries,including blackcurrant,aromia,bilberry and ...This study aims to investigate the protective effects of BlingLife®-berry extract on the eyes.BlingLife®-berry extract is a mixture of high-quality natural berries,including blackcurrant,aromia,bilberry and maquiberry.The main active ingredient responsible for theeye-protective effects is anthocyanins.Anthocyanins are natural water-soluble pigments belonging to the flavonoid class,and theyhave multiple benefits,including improving vision,anti-inflammatory and antibacterial properties,and antioxidant effects.Dueto the dual benefits of anthocyanins in protecting vision and eliminating free radicals,this study explores the comprehensive eye protective effects of BlingLife®-berry extract in terms of combating blue light-induced eye damage and oxidative stress-inducedvisual fatigue.The results provide robust evidence for the in vivo effects and further technological applications of BlingLife®-berry extract.展开更多
To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith (AGS), the crystal structure was measured with a Bruker APEX-Ⅱ area-...To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith (AGS), the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ (ECHS) was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters: a=(8.3652±0.0006) nm, b=(24.721±0.002) nm, and c=(14.5587±0.0011) nm, α=90°, β=97.850 (4) °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number (Z) of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology (CCD-RSM) was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows: for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.展开更多
One of the most important factors limiting the success of plant-derived therapeutics is that these products are generally a mixture containing many different molecules.The compounds responsible for bioactivities and/o...One of the most important factors limiting the success of plant-derived therapeutics is that these products are generally a mixture containing many different molecules.The compounds responsible for bioactivities and/or clinical effects are also not yet identified.To overcome this hurdle,we investigated whether we can reconstitute bioactivities展开更多
文摘[Objective] TO study the combined extracting process of porcine blood superoxide dismutase (SOD) and other bioactive substances and thus to provide technical basis for making full use of blood resources and large-scale production of SOD. [Method] Fibronectin, immunoglobulin, and hemoglobin were isolated from porcine blood, and SOD was extracted. Trace pyrogallol self-oxidation method to determine SOD activity was modified by optimizing the volume of pyrogallol and SOD samples, reaction temperature, and buffer pH. The specific activity of SOD was determined with the optimized extraction conditions. [ Result] The improved experimental conditions of SOD activity detection were as follows: 7 pyrogallol (50 mmol/L), 3 ml Tris-HCI (50 mmol/L, pH 8.2), reactive temperature at 25(3, and 10 pl SOD sample solution. The specific activity of extracted SOD was 5 056 U/mg protein. [ Conclusion] Four kinds of bioactive substance can be isolated from porcine blood by modern biological engi- neering integration technology, and the extracted SOD has better activity.
文摘This study aims to investigate the protective effects of BlingLife®-berry extract on the eyes.BlingLife®-berry extract is a mixture of high-quality natural berries,including blackcurrant,aromia,bilberry and maquiberry.The main active ingredient responsible for theeye-protective effects is anthocyanins.Anthocyanins are natural water-soluble pigments belonging to the flavonoid class,and theyhave multiple benefits,including improving vision,anti-inflammatory and antibacterial properties,and antioxidant effects.Dueto the dual benefits of anthocyanins in protecting vision and eliminating free radicals,this study explores the comprehensive eye protective effects of BlingLife®-berry extract in terms of combating blue light-induced eye damage and oxidative stress-inducedvisual fatigue.The results provide robust evidence for the in vivo effects and further technological applications of BlingLife®-berry extract.
基金Project(11JJ2042)supported by the Natural Science Foundation of Hunan Province,ChinaProject supported by the "Twelfth Five-Year" Key Discipline of Hunan University of Chinese Medicine-Pharmaceutical Analysis Science,China+1 种基金Project(11K048)supported by the Innovation Platform and Open Foundation Program of Higher Colleges of Hunan Province,ChinaProject(K1207010-21)supported by the Changsha City Science and Technology Bureau Key Projects,China
文摘To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith (AGS), the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ (ECHS) was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters: a=(8.3652±0.0006) nm, b=(24.721±0.002) nm, and c=(14.5587±0.0011) nm, α=90°, β=97.850 (4) °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number (Z) of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology (CCD-RSM) was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows: for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.
文摘One of the most important factors limiting the success of plant-derived therapeutics is that these products are generally a mixture containing many different molecules.The compounds responsible for bioactivities and/or clinical effects are also not yet identified.To overcome this hurdle,we investigated whether we can reconstitute bioactivities