僵尸网络(Botnet)是一种从传统恶意代码形态进化而来的新型攻击方式,为攻击者提供了隐匿、灵活且高效的一对多命令与控制信道(Command and Control channel,C&C)机制,可以控制大量僵尸主机实现信息窃取、分布式拒绝服务攻击和垃圾...僵尸网络(Botnet)是一种从传统恶意代码形态进化而来的新型攻击方式,为攻击者提供了隐匿、灵活且高效的一对多命令与控制信道(Command and Control channel,C&C)机制,可以控制大量僵尸主机实现信息窃取、分布式拒绝服务攻击和垃圾邮件发送等攻击目的。该文提出一种与僵尸网络结构和C&C协议无关,不需要分析数据包的特征负载的僵尸网络检测方法。该方法首先使用预过滤规则对捕获的流量进行过滤,去掉与僵尸网络无关的流量;其次对过滤后的流量属性进行统计;接着使用基于X-means聚类的两步聚类算法对C&C信道的流量属性进行分析与聚类,从而达到对僵尸网络检测的目的。实验证明,该方法高效准确地把僵尸网络流量与其他正常网络流量区分,达到从实际网络中检测僵尸网络的要求,并且具有较低的误判率。展开更多
The subversive nature of information war lies not only in the information itself, but also in the circulation and application of information. It has always been a challenge to quantitatively analyze the function and e...The subversive nature of information war lies not only in the information itself, but also in the circulation and application of information. It has always been a challenge to quantitatively analyze the function and effect of information flow through command, control, communications, computer, kill, intelligence,surveillance, reconnaissance (C4KISR) system. In this work, we propose a framework of force of information influence and the methods for calculating the force of information influence between C4KISR nodes of sensing, intelligence processing,decision making and fire attack. Specifically, the basic concept of force of information influence between nodes in C4KISR system is formally proposed and its mathematical definition is provided. Then, based on the information entropy theory, the model of force of information influence between C4KISR system nodes is constructed. Finally, the simulation experiments have been performed under an air defense and attack scenario. The experimental results show that, with the proposed force of information influence framework, we can effectively evaluate the contribution of information circulation through different C4KISR system nodes to the corresponding tasks. Our framework of force of information influence can also serve as an effective tool for the design and dynamic reconfiguration of C4KISR system architecture.展开更多
针对液晶显示器(LCD)面板的“Chip/FPC on Glass”(C/FOG)工艺生产制造过程中存在的计量延迟大、生产异常无法提前预测的问题,本文提出一种基于神经网络的C/FOG工艺生产制造虚拟计量方法。该方法利用生产机台上的传感器采集生产过程中...针对液晶显示器(LCD)面板的“Chip/FPC on Glass”(C/FOG)工艺生产制造过程中存在的计量延迟大、生产异常无法提前预测的问题,本文提出一种基于神经网络的C/FOG工艺生产制造虚拟计量方法。该方法利用生产机台上的传感器采集生产过程中的过程状态数据,构建基于多尺度一维卷积及通道注意力模型(MS1DC-CA)的虚拟计量模型。通过多个尺度的卷积核提取不同尺度范围内的状态数据特征。在对含有缺失值的原始数据预处理中,提出了基于粒子群算法改进的K近邻填补方法(PSO-KNN Imputation)进行缺失值填充,保留特征的同时,减少因填充值引入的干扰。最后在实际生产采集的数据上进行实验对比分析,实际不良率主要集中在0.1%~0.5%,该虚拟计量模型的拟合均方误差为0.397 7‱,低于其他现有拟合模型,在平均绝对误差、对称平均绝对百分比误差和拟合优度3种评价指标下也均优于其他现有的拟合模型,具有良好的预测性能。展开更多
The effects of ATP-sensitive mitochondrial K + channel(mitoK ATP) on mitochondrial membrane potential(Δψm),cell proliferation and protein kinase C alpha(PKCα) expression in airway smooth muscle cells(ASMCs) were in...The effects of ATP-sensitive mitochondrial K + channel(mitoK ATP) on mitochondrial membrane potential(Δψm),cell proliferation and protein kinase C alpha(PKCα) expression in airway smooth muscle cells(ASMCs) were investigated.Thirty-six Sprague-Dawley(SD) rats were immunized with saline(controls) or ovalbumin(OVA) with alum(asthma models).ASMCs were cultured from the lung of control and asthma rats.ASMCs were treated with diazoxide(the potent activator of mitoK ATP) or 5-hydroxydencanote(5-HD,the inhibitor of mitoK ATP).Rhodamine-123(R-123) was used to detect Δψm.The expression of PKCα protein was examined by using Western blotting,while PKCα mRNA expression was detected by using real-time PCR.The proliferation of ASMCs was measured by MTT assay and cell cycle analysis.In diazoxide-treated normal ASMCs,the R-123 fluorescence intensity,protein and mRNA levels of PKCα,MTT A values and percentage of cells in S phase were markedly increased as compared with untreated controls.The ratio of G 0 /G 1 cells was decreased(P<0.05) in diazoxide-treated ASMCs from normal rats.However,there were no significant differences between the ASMCs from healthy rats treated with 5-HD and the normal control group.In untreated and diazoxide-treated ASMCs of asthmatic rats,the R-123 fluorescence intensity,protein and mRNA levels of PKCα,MTT A values and the percentage of cells in S phase were increased in comparison to the normal control group.Furthermore,in comparison to ASMCs from asthmatic rats,these values were considerably increased in asthmatic group treated with diazoxide(P<0.05).After exposure to 5-HD for 24 h,these values were decreased as compared with asthma control group(P<0.05).In ASMCs of asthma,the signal transduction pathway of PKCα may be involved in cell proliferation,which is induced by the opening of mitoK ATP and the depolarization of Δψm.展开更多
Using whole-cell patch-clamp recordings, the effects of antigliomatin were observed on chloride channels on C6 glioma cells cultured in vitro. Antigliomatin was extracted from the venom of the scorpion Buthus martensi...Using whole-cell patch-clamp recordings, the effects of antigliomatin were observed on chloride channels on C6 glioma cells cultured in vitro. Antigliomatin was extracted from the venom of the scorpion Buthus martensii Karsch. Chloride channels are closed under normal osmotic pressure. When osmotic pressure was reduced to 120, 110 and 100 mV, the cell volume enlarged, chloride channels opened, and the chloride channel current increased. Three minutes after antigliomatin treatment, the chloride channel current decreased in a dose-dependent manner. These results show that antigliomatin extracted from the venom of the scorpion Buthus martensii Karsch diminishes chloride channel currents on C6 glioma cells.展开更多
BACKGROUND Diabetic cardiomyopathy(DCM) is a serious complication of end-stage diabetes that presents symptoms such as cardiac hypertrophy and heart failure. The transient receptor potential channel 6(TRPC6) protein i...BACKGROUND Diabetic cardiomyopathy(DCM) is a serious complication of end-stage diabetes that presents symptoms such as cardiac hypertrophy and heart failure. The transient receptor potential channel 6(TRPC6) protein is a very important selective calcium channel that is closely related to the development of various cardiomyopathies.AIM To explore whether TRPC6 affects cardiomyocyte apoptosis and proliferation inhibition in DCM.METHODS We compared cardiac function and myocardial pathological changes in wild-type mice and mice injected with streptozotocin(STZ), in addition to comparing the expression of TRPC6 and P-calmodulin-dependent protein kinase Ⅱ(P-CaMKⅡ) in them. At the same time, we treated H9C2 cardiomyocytes with high glucose and then evaluated the effects of addition of SAR, a TRPC6 inhibitor, and KN-93, a CaMKⅡ inhibitor, to such H9C2 cells in a high-glucose environment.RESULTS We found that STZ-treated mice had DCM, decreased cardiac function, necrotic cardiomyocytes, and limited proliferation. Western blot and immunofluorescence were used to detect the expression levels of various appropriate proteins in the myocardial tissue of mice and H9C2 cells. Compared to those in the control group, the expression levels of the apoptosis-related proteins cleaved caspase 3 and Bax were significantly higher in the experimental group, while the expression of the proliferation-related proteins proliferating cell nuclear antigen(PCNA) and CyclinD1 was significantly lower. In vivo and in vitro, the expression of TRPC6 and P-CaMKⅡ increased in a high-glucose environment. However, addition of inhibitors to H9C2 cells in a high-glucose environment resulted in alleviation of both apoptosis and proliferation inhibition.CONCLUSION The inhibition of apoptosis and proliferation of cardiomyocytes in a high-glucose environment may be closely related to activation of the TRPC6/P-CaMKⅡ pathway.展开更多
The effects of protein kinase C (PKC) on the tension and the activity of voltage-dependent delayed rectifier potassium channel (K,,) were examined in normal and passively sensitized human airway smooth muscle (H...The effects of protein kinase C (PKC) on the tension and the activity of voltage-dependent delayed rectifier potassium channel (K,,) were examined in normal and passively sensitized human airway smooth muscle (HASM), by measuring tones and whole-cell patch clamp techniques, and the Kv activities and membrane potential (Em) were also detected. The results showed that phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a concentration-dependent constriction in normal HASM rings. The constriction of the passively sensitized muscle in asthma serum group was significantly higher than that of the normal group (P〈0.05), and the constrictions of both groups were completely abolished by PKC inhibitor Ro31-8220 and calcium channel inhibitor nifedipine. Kv activities of HASM cells were significantly inhibited by PMA, and the Em became more positive, as compared with the DMSO (a PMA menstruum)-treated group (P〈0.01). This effect could be blocked by Ro31-8220 (P〈0.01 ). It was concluded that activation of PKC could increase the tones of HASM, which might be related to the reduced Kv activity. In passively sensitized HASM rings, this effect was more notable.展开更多
The first important problem in the star forming process is the formation of proto star core in star forming regions of molecular cloud. The multi core structure in star forming regions is related to the forming of pro...The first important problem in the star forming process is the formation of proto star core in star forming regions of molecular cloud. The multi core structure in star forming regions is related to the forming of proto star core. The molecular radiation of C 18 O( J = 1-0) in Cepheus C has been observed. The C 18 O( J = 1-0) observations form the basis for an interesting study on the cloud cores and star formation activity in the cores of the Cepheus C. In order to study the multi core structure of C 18 O( J = 1-0) in the Cepheus C the channel maps and the position velocity diagrams of C 18 O( J = 1-0) will be shown. From the maps it is found that the contour level and distribution size of the three cores in Cepheus C are related to the channel velocity very much. The channel velocity of C 18 O( J = 1-0) molecules in core b, which distributed in all the channels velocity, is different with one in core a and core c very much. The C 18 O( J = 1-0) molecules in core a and core c of the Cepheus C mostly distributed in the blue shifted channel velocity relating to peak velocity, and only in -10.0 ~ -9.5 km/s, which is the red shifted channel velocity relating to peak velocity. And the contour level of C 18 O( J = 1-0) in -10.0 ~ -9.5 km/s is small and the distrbution size in the channel map is small. According to the position velocity diagrams the asymmetry of the distribution both blue shifted and red shifted components should reflect the asymmetry of the profile. From the diagrams it also is found that the contour level and the distribution size of the three cores are different from each other. Both results from the maps and diagrams are coincident with each other.展开更多
针对城市场景的蜂窝车联网(cellular vehicle to everything,C-V2X)中车辆缓存节点高效选择和合理的缓存文件分配问题,提出了基于跨层信息的C-V2X缓存分配算法(Cross-layer Caching Allocation for C-V2X,CLCA)。该算法在LTE-V2X mode3...针对城市场景的蜂窝车联网(cellular vehicle to everything,C-V2X)中车辆缓存节点高效选择和合理的缓存文件分配问题,提出了基于跨层信息的C-V2X缓存分配算法(Cross-layer Caching Allocation for C-V2X,CLCA)。该算法在LTE-V2X mode3模式下,由基站周期地收集覆盖范围内节点的连接度、信噪比和信道接入时延信息,并重新划分邻居归属,构建跨层信息邻接矩阵,再采用贪婪分配的方法筛选缓存节点,以减少缓存节点数量,节约通信资源并降低开销。进而,以服从Zipf分布的文件流行度分布为基础,采用文件流行度预测概率与历史周期实际流行度概率加权叠加的方式,提高文件流行度预测的准确性,再根据缓存容量合理配置缓存文件。仿真分析表明,该算法可以有效提高节点的文件获取效率,降低文件传输时延,减少基站负载。展开更多
文摘僵尸网络(Botnet)是一种从传统恶意代码形态进化而来的新型攻击方式,为攻击者提供了隐匿、灵活且高效的一对多命令与控制信道(Command and Control channel,C&C)机制,可以控制大量僵尸主机实现信息窃取、分布式拒绝服务攻击和垃圾邮件发送等攻击目的。该文提出一种与僵尸网络结构和C&C协议无关,不需要分析数据包的特征负载的僵尸网络检测方法。该方法首先使用预过滤规则对捕获的流量进行过滤,去掉与僵尸网络无关的流量;其次对过滤后的流量属性进行统计;接着使用基于X-means聚类的两步聚类算法对C&C信道的流量属性进行分析与聚类,从而达到对僵尸网络检测的目的。实验证明,该方法高效准确地把僵尸网络流量与其他正常网络流量区分,达到从实际网络中检测僵尸网络的要求,并且具有较低的误判率。
基金supported by the Natural Science Foundation Research Plan of Shanxi Province (2023JCQN0728)。
文摘The subversive nature of information war lies not only in the information itself, but also in the circulation and application of information. It has always been a challenge to quantitatively analyze the function and effect of information flow through command, control, communications, computer, kill, intelligence,surveillance, reconnaissance (C4KISR) system. In this work, we propose a framework of force of information influence and the methods for calculating the force of information influence between C4KISR nodes of sensing, intelligence processing,decision making and fire attack. Specifically, the basic concept of force of information influence between nodes in C4KISR system is formally proposed and its mathematical definition is provided. Then, based on the information entropy theory, the model of force of information influence between C4KISR system nodes is constructed. Finally, the simulation experiments have been performed under an air defense and attack scenario. The experimental results show that, with the proposed force of information influence framework, we can effectively evaluate the contribution of information circulation through different C4KISR system nodes to the corresponding tasks. Our framework of force of information influence can also serve as an effective tool for the design and dynamic reconfiguration of C4KISR system architecture.
文摘针对液晶显示器(LCD)面板的“Chip/FPC on Glass”(C/FOG)工艺生产制造过程中存在的计量延迟大、生产异常无法提前预测的问题,本文提出一种基于神经网络的C/FOG工艺生产制造虚拟计量方法。该方法利用生产机台上的传感器采集生产过程中的过程状态数据,构建基于多尺度一维卷积及通道注意力模型(MS1DC-CA)的虚拟计量模型。通过多个尺度的卷积核提取不同尺度范围内的状态数据特征。在对含有缺失值的原始数据预处理中,提出了基于粒子群算法改进的K近邻填补方法(PSO-KNN Imputation)进行缺失值填充,保留特征的同时,减少因填充值引入的干扰。最后在实际生产采集的数据上进行实验对比分析,实际不良率主要集中在0.1%~0.5%,该虚拟计量模型的拟合均方误差为0.397 7‱,低于其他现有拟合模型,在平均绝对误差、对称平均绝对百分比误差和拟合优度3种评价指标下也均优于其他现有的拟合模型,具有良好的预测性能。
基金supported by grants from Natural Science Foundation of Hubei Province,China (No. 2010CDB096)the National Key Technology R&D Program of the 12th National Five-year Development Plan of China (No. 2012BAI05B01)
文摘The effects of ATP-sensitive mitochondrial K + channel(mitoK ATP) on mitochondrial membrane potential(Δψm),cell proliferation and protein kinase C alpha(PKCα) expression in airway smooth muscle cells(ASMCs) were investigated.Thirty-six Sprague-Dawley(SD) rats were immunized with saline(controls) or ovalbumin(OVA) with alum(asthma models).ASMCs were cultured from the lung of control and asthma rats.ASMCs were treated with diazoxide(the potent activator of mitoK ATP) or 5-hydroxydencanote(5-HD,the inhibitor of mitoK ATP).Rhodamine-123(R-123) was used to detect Δψm.The expression of PKCα protein was examined by using Western blotting,while PKCα mRNA expression was detected by using real-time PCR.The proliferation of ASMCs was measured by MTT assay and cell cycle analysis.In diazoxide-treated normal ASMCs,the R-123 fluorescence intensity,protein and mRNA levels of PKCα,MTT A values and percentage of cells in S phase were markedly increased as compared with untreated controls.The ratio of G 0 /G 1 cells was decreased(P<0.05) in diazoxide-treated ASMCs from normal rats.However,there were no significant differences between the ASMCs from healthy rats treated with 5-HD and the normal control group.In untreated and diazoxide-treated ASMCs of asthmatic rats,the R-123 fluorescence intensity,protein and mRNA levels of PKCα,MTT A values and the percentage of cells in S phase were increased in comparison to the normal control group.Furthermore,in comparison to ASMCs from asthmatic rats,these values were considerably increased in asthmatic group treated with diazoxide(P<0.05).After exposure to 5-HD for 24 h,these values were decreased as compared with asthma control group(P<0.05).In ASMCs of asthma,the signal transduction pathway of PKCα may be involved in cell proliferation,which is induced by the opening of mitoK ATP and the depolarization of Δψm.
基金the Science and Technology Development Program of Jilin Province, No.20050407-6
文摘Using whole-cell patch-clamp recordings, the effects of antigliomatin were observed on chloride channels on C6 glioma cells cultured in vitro. Antigliomatin was extracted from the venom of the scorpion Buthus martensii Karsch. Chloride channels are closed under normal osmotic pressure. When osmotic pressure was reduced to 120, 110 and 100 mV, the cell volume enlarged, chloride channels opened, and the chloride channel current increased. Three minutes after antigliomatin treatment, the chloride channel current decreased in a dose-dependent manner. These results show that antigliomatin extracted from the venom of the scorpion Buthus martensii Karsch diminishes chloride channel currents on C6 glioma cells.
文摘BACKGROUND Diabetic cardiomyopathy(DCM) is a serious complication of end-stage diabetes that presents symptoms such as cardiac hypertrophy and heart failure. The transient receptor potential channel 6(TRPC6) protein is a very important selective calcium channel that is closely related to the development of various cardiomyopathies.AIM To explore whether TRPC6 affects cardiomyocyte apoptosis and proliferation inhibition in DCM.METHODS We compared cardiac function and myocardial pathological changes in wild-type mice and mice injected with streptozotocin(STZ), in addition to comparing the expression of TRPC6 and P-calmodulin-dependent protein kinase Ⅱ(P-CaMKⅡ) in them. At the same time, we treated H9C2 cardiomyocytes with high glucose and then evaluated the effects of addition of SAR, a TRPC6 inhibitor, and KN-93, a CaMKⅡ inhibitor, to such H9C2 cells in a high-glucose environment.RESULTS We found that STZ-treated mice had DCM, decreased cardiac function, necrotic cardiomyocytes, and limited proliferation. Western blot and immunofluorescence were used to detect the expression levels of various appropriate proteins in the myocardial tissue of mice and H9C2 cells. Compared to those in the control group, the expression levels of the apoptosis-related proteins cleaved caspase 3 and Bax were significantly higher in the experimental group, while the expression of the proliferation-related proteins proliferating cell nuclear antigen(PCNA) and CyclinD1 was significantly lower. In vivo and in vitro, the expression of TRPC6 and P-CaMKⅡ increased in a high-glucose environment. However, addition of inhibitors to H9C2 cells in a high-glucose environment resulted in alleviation of both apoptosis and proliferation inhibition.CONCLUSION The inhibition of apoptosis and proliferation of cardiomyocytes in a high-glucose environment may be closely related to activation of the TRPC6/P-CaMKⅡ pathway.
基金This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30270583)
文摘The effects of protein kinase C (PKC) on the tension and the activity of voltage-dependent delayed rectifier potassium channel (K,,) were examined in normal and passively sensitized human airway smooth muscle (HASM), by measuring tones and whole-cell patch clamp techniques, and the Kv activities and membrane potential (Em) were also detected. The results showed that phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a concentration-dependent constriction in normal HASM rings. The constriction of the passively sensitized muscle in asthma serum group was significantly higher than that of the normal group (P〈0.05), and the constrictions of both groups were completely abolished by PKC inhibitor Ro31-8220 and calcium channel inhibitor nifedipine. Kv activities of HASM cells were significantly inhibited by PMA, and the Em became more positive, as compared with the DMSO (a PMA menstruum)-treated group (P〈0.01). This effect could be blocked by Ro31-8220 (P〈0.01 ). It was concluded that activation of PKC could increase the tones of HASM, which might be related to the reduced Kv activity. In passively sensitized HASM rings, this effect was more notable.
文摘The first important problem in the star forming process is the formation of proto star core in star forming regions of molecular cloud. The multi core structure in star forming regions is related to the forming of proto star core. The molecular radiation of C 18 O( J = 1-0) in Cepheus C has been observed. The C 18 O( J = 1-0) observations form the basis for an interesting study on the cloud cores and star formation activity in the cores of the Cepheus C. In order to study the multi core structure of C 18 O( J = 1-0) in the Cepheus C the channel maps and the position velocity diagrams of C 18 O( J = 1-0) will be shown. From the maps it is found that the contour level and distribution size of the three cores in Cepheus C are related to the channel velocity very much. The channel velocity of C 18 O( J = 1-0) molecules in core b, which distributed in all the channels velocity, is different with one in core a and core c very much. The C 18 O( J = 1-0) molecules in core a and core c of the Cepheus C mostly distributed in the blue shifted channel velocity relating to peak velocity, and only in -10.0 ~ -9.5 km/s, which is the red shifted channel velocity relating to peak velocity. And the contour level of C 18 O( J = 1-0) in -10.0 ~ -9.5 km/s is small and the distrbution size in the channel map is small. According to the position velocity diagrams the asymmetry of the distribution both blue shifted and red shifted components should reflect the asymmetry of the profile. From the diagrams it also is found that the contour level and the distribution size of the three cores are different from each other. Both results from the maps and diagrams are coincident with each other.
文摘针对城市场景的蜂窝车联网(cellular vehicle to everything,C-V2X)中车辆缓存节点高效选择和合理的缓存文件分配问题,提出了基于跨层信息的C-V2X缓存分配算法(Cross-layer Caching Allocation for C-V2X,CLCA)。该算法在LTE-V2X mode3模式下,由基站周期地收集覆盖范围内节点的连接度、信噪比和信道接入时延信息,并重新划分邻居归属,构建跨层信息邻接矩阵,再采用贪婪分配的方法筛选缓存节点,以减少缓存节点数量,节约通信资源并降低开销。进而,以服从Zipf分布的文件流行度分布为基础,采用文件流行度预测概率与历史周期实际流行度概率加权叠加的方式,提高文件流行度预测的准确性,再根据缓存容量合理配置缓存文件。仿真分析表明,该算法可以有效提高节点的文件获取效率,降低文件传输时延,减少基站负载。