A Single-Cell Landscape of Human Liver Transplantation Reveals a Pathogenic Immune Niche Associated with Early Allograft Dysfunction

Liver transplantation(LT)is the standard therapy for individuals afflicted with end-stage liver disease.Despite notable advancements in LT technology,the incidence of early allograft dysfunction(EAD)remains a critical concern,exacerbating the current organ shortage and detrimentally affecting the prognosis of recipients.Unfortunately,the perplexing hepatic heterogeneity has impeded characterization of the cellular traits and molecular events that contribute to EAD.Herein,we constructed a pioneering single-cell transcriptomic landscape of human transplanted livers derived from non-EAD and EAD patients,with 12 liver samples collected from 7 donors during the cold perfusion and portal reperfusion stages.Comparison of the 75231 cells of non-EAD and EAD patients revealed an EAD-associated immune niche comprising mucosal-associated invariant T cells,granzyme B^(+)(GZMB^(+))granzyme K^(+)(GZMK^(+))natural killer cells,and S100 calcium binding protein A12^(+)(S100A12^(+))neutrophils.Moreover,we verified this immune niche and its association with EAD occurrence in two independent cohorts.Our findings elucidate the cellular characteristics of transplanted livers and the EAD-associated pathogenic immune niche at the single-cell level,thus,offering valuable insights into EAD onset.

The International Communication of Chinese Concept of Human Rights in the New Era

The Chinese concept of human rights serves as the core value of China’s path of human rights development,guiding the comprehensive advancement of China’s human rights cause and providing Chinese wisdom for the development of human rights cause worldwide.Throughout the development process of China’s human rights cause in the new era,the international communication of the Chinese concept of human rights has achieved positive results and played an essential role in the international human rights discourse struggle,external exchanges on human rights,and the transformation of international human rights mechanisms.However,it also faces challenges such as the dominance of Western human rights concepts,the marginalization of the Chinese concept of human rights,the incomplete establishment of China’s external discourse system on human rights,and constraints on international communication channels.Therefore,in the new era,it is crucial to address the weaknesses in the international communication of the Chinese concept of human rights,optimize communication channels,and enhance its international influence.

Functions of Rhetoric and Social Science Origins in Communication Study—Based on Boxing Plato’s Shadow:An Introduction to the Study of Human Communication

With the development of the rhetoric study from the origins in ancient Athens and The Rhetoric of Aristotle to the formation of modern Communication Study at the beginning of the twentieth century,the Rhetoric Study changed in dynamic and its concepts and theories merged and formed the foundations of Communication Study which focused on personal communication and speech rhetoric skills at that time.At the beginning of the twentieth century,although the Neo-Aristotelian rhetorical scholars from classic rhetoric approach created methodologies and enriched the content of Communication Study under the influence of humanistic study and social science study,but the Communication discipline was still in the embarrassing position.During World War I and World War II,most scholars with backgrounds of social science,history and anthropology carried out the studies of propaganda wars and the message disseminating,and explored the military powers and governments’access to the media and developed serial communication modes from the social science perspective,which laid the foundation for Communication discipline in the academic field.The author analyzed the Rhetoric and Social Science origins of Communication study in Boxing Plato’s Shadow:an Introduction to the Study of Human Communication,which is beneficial and helpful to understand the academic origins of Communication study and its cross-disciplined and embarrassing positions.

The Three-dimensional Model of “ConstructionTranslation-Communication” for the Construction of a Human Rights Discourse System with Chinese Characteristics in the Post-pandemic Era

Human rights have always been the focus of disputes between China and the West. To make a better China story about human rights and enhance China’s international discourse power in the field of human rights, we propose the text construction, translation and communication path of the construction of the human rights discourse system with Chinese characteristics from the cross-disciplinary perspective. Based on the examples of prevention and control of the CoVID-19 pandemic under the concept of putting people first, mainstream diplomatic linguistic data relevant to the fight against the novel coronavirus was collected through the internet. The human rights discourse with Chinese characteristics is constructed through a variety of texts focusing on putting the people first, the right to life, the right to health, the right to information, the right to privacy, the right to work and a sense of a community with a shared future for human beings. The core concepts of human rights have experienced the deepening process of decontextualization, abstraction and theorization to gradually conform to the expression habits of international audiences. The international communication effect is mainly positive in civil society. It shows that the construction of China’s human rights discourse system and the governance of international human rights discourse have achieved preliminary results. To enhance China’s discourse power on human rights, we need to add an interdisciplinary approach.

Globalization and Human Rights——An interview with Associate Researcher Xu Tiebing with the Communication University of China

Human fights has always been a concept that evolves with the development of globalization. To clarify their relations, Human Rights made an interview with Xu Tiebing, an associate researcher on international studies with the Communication University of China. Xu has recently translated the French book Atlas de la Mondialisation into Chinese.

Shipborne Underwater Acoustic Communication System and Sea Trials with Submersible Shenhai Yongshi

The Shipborne acoustic communication system of the submersible Shenhai Yongshi works in vertical, horizontal and slant channels according to the relative positions. For ease of use, an array combined by a vertical-cone directional transducer and a horizontal-toroid one is installed on the mothership. Improved techniques are proposed to combat adverse channel conditions, such as frequency selectivity, non-stationary ship noise, and Doppler effects of the platform’s nonlinear movement. For coherent modulation, a turbo-coded single-carrier scheme is used. In the receiver, the sparse decision-directed Normalized Least-Mean-Square soft equalizer automatically adjusts the tap pattern and weights according to the multipath structure, the two receivers’ asymmetry, the signal’s frequency selectivity and the noise’s spectrum fluctuation. The use of turbo code in turbo equalization significantly suppresses the error floor and decreases the equalizer’s iteration times, which is verified by both the extrinsic information transfer charts and bit-error-rate performance. For noncoherent modulation, a concatenated error correction scheme of nonbinary convolutional code and Hadamard code is adopted to utilize full frequency diversity. Robust and lowcomplexity synchronization techniques in the time and Doppler domains are proposed. Sea trials with the submersible to a maximum depth of over 4500 m show that the shipborne communication system performs robustly during the adverse conditions. From the ten-thousand communication records in the 28 dives in 2017, the failure rate of the coherent frames and that of the noncoherent packets are both below 10%, where both synchronization errors and decoding errors are taken into account.

Up-Regulation of the Gap Junction Intercellular Communication by Tea Polyphenol in the Human Metastatie Lung Carcinoma Cell Line

Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.

Humanity factors and more: A little heads up for medical students and future doctors

Exactly how can we become a successful doctor in the future? It has been widely accepted that chasing perfection in our field of expertise alone is not enough. Here in China, doctors have the liability to do more. Chinese doctors are relatively more prone to confronting difficult situations in a more complex manner. Therefore, the humanity factor in medical practice is discussed, and some essential communication skills are suggested for medical practitioners, with heartfelt anticipation for a better medical environment as well as a more pleasant Doctor-Patient relationship.

Energy intake,metabolic homeostasis,and human health

The energy substances(mainly carbohydrates and fats)are the basis and guarantee of life activity,especially the oxidative phosphorylation for energy supply.However,excessive absorption and accumulation of these substances can lead to metabolic diseases such as obesity,hyperlipidemia,diabetes,and cancers.A large amount of studies demonstrate that G protein-coupled receptors(GPCRs)play a key role in identification and absorption of energy substances,and the signaling network of nerves,immune,and endocrine regulates their storage and utilization.The gastrointestinal mucus layer not only identifies these substances through identification in diet components but also transfers immune,metabolic,and endocrine signals of hormones,cytokines,and chemokines by promoting interactions between receptors and ligands.These signaling molecules are transferred to corresponding organs,tissues,and cells by the circulatory system,and cell activity is regulated by amplifying of cell signals that constitute the wireless communication network among cells in the body.Absorption,accumulation,and utilization of energy substances in the body obey the law of energy conservation.Energy is stored in the form of fat,and meets the demand of body via two coupled mechanisms:catabolism and oxidative phosphorylation.Under normal physiological conditions,fat consumption involves ketone body metabolism through the circulatory system and glucose consumption requires blood lactic acid cycle.Accumulation of excessive energy leads to the abnormal activation of mammalian target of rapamycin(mTOR),thus promoting the excretion of glucose or glycogen in the form of blood glucose and urine glucose.Alternatively,the body cancels the intercellular contact inhibition and promotes cell proliferation to induce carcinogenesis,which can induce the consumption of large amounts of glucose.Intercellular communication is performed by signaling molecules via sensing,absorption,accumulation,and utilization of energy substances,and anabolism and catabolism are controlled by the central metabolic pathway.Therefore,slower catabolism will result in longer life expectancy,whereas faster catabolism results in shorter life expectancy.Energy substances in diet influence the balance between energy and metabolism in the body through the sensing function of the gastrointestinal system at two levels:cellular communication network and metabolic network.The present review of studies aims to strengthen our knowledge on cellular communication and metabolic networks to offer a dietary guidance on the metabolism and communication role of various foods.

Relevance of Visual, Auditory, and Olfactory Cues in Pet Dogs’ Awareness of Humans

Many studies have looked at how dogs respond to human communicative information. Here, we examined which human communicative factors were important in influencing dogs’ responses. Eleven healthy pet dogs with no apparent aggressive behaviour toward people were recruited. Five sensory conditions (all cues presented;either a visual, an auditory, or an olfactory cue presented;no cues presented) were provided three times randomly to each dog during the tests. All tests were video recorded, and both the dogs’ behaviour and time taken to reach the person when she presented each of the sensory cue conditions were observed. Total rates of reaching the person were as follows: 97.0% (all cues), 87.9% (auditory cues), 84.4% (visual cues), 84.4% (olfactory cues), and 69.7% (no cues). The time taken for the dog to notice the person in the box and then obtain a reward from her differed among the five conditions: all cues (6.00 ± 0.32 s) and visual cues (6.02 ± 0.91 s) were significantly faster than auditory cues (18.56 ± 9.57 s) and no cues (26.55 ± 11.72 s). Thus the type of information input was important in recognition of the person by the dogs and influenced the dogs’ response times;visual cues appeared advantageous in confirming the person’s presence.

Basic Investigations EXPRESSION OF GAP JUNCTION PROTEIN Cx43 IN CULTURED HUMAN NORMAL AND MALIGNANT LUNG CELLS

Gap junctional intercellular communicationexchange of small molecules and ions between contiguous cells through membranous gap junctional channelsis essential for growth control and tissue homecotasis. This work concerns the functional expression of gap junction protein connexin 43 (Cx43) in normal human lung cells and the changes in lung carcinoma cells. By. using Northern blot hybridization analysis and Cx43 immunocytochemical methods, it was otherved that cultured normal human embryonic lung cells expressed a high level of Cx43 in both mRNA and protein levels.The Cx43 immunofluorescence was localized at cell membrane regions corresponding to the location of gap junctions. These normal lung cells were competent of intercellular communication function as detected by Lucifer yellow dye transfer. In contrast to normal celis, Cx43 mRNA and protein was not detectable in the carcinoma PG cell line. These tumor cells were defective of intercellular communication function. These results demonstrate that Cx43 is expressed in normal cultured human embryonic lung cells but not in lung tumor cells. The lack of intercellular communication in the lung tumor cell line correlates with dysfunctional intercellular communication. The suggestive role of Cx as a tumor suppersor gene is discussed.

Magnetized Micropillar-Enabled Wearable Sensors for Touchless and Intelligent Information Communication

The wearable sensors have recently attracted considerable attentions as communication interfaces through the information perception,decoding,and conveying process.However,it is still challenging to obtain a sensor that can convert detectable signals into multiple outputs for convenient,e cient,cryptic,and high-capacity information transmission.Herein,we present a capacitive sensor of magnetic field based on a tilted flexible micromagnet array(t-FMA)as the proposed interaction interface.With the bidirectional bending capability of t-FMA actuated by magnetic torque,the sensor can recognize both the magnitude and orientation of magnetic field in real time with non-overlapping capacitance signals.The optimized sensor exhibits the high sensitivity of over 1.3 T-1 and detection limit down to 1 mT with excellent durability.As a proof of concept,the sensor has been successfully demonstrated for convenient,e cient,and programmable interaction systems,e.g.,touchless Morse code and Braille communication.The distinguishable recognition of the magnetic field orientation and magnitude further enables the sensor unit as a high-capacity transmitter for cryptic information interaction(e.g.,encoded ID recognition)and multi-control instruction outputting.We believe that the proposed magnetic field sensor can open up a potential avenue for future applications including information communication,virtual reality device,and interactive robotics.

A 2Mbps Human Body Communication Transceiver Based on Body Antenna Effect

This paper presents a human body communication(HBC)transceiver for wireless body network applications.The transceiver employs on frequency shift keying(FSK)modulation and op-erates in 40 MHz-60 MHz which is the resonant frequency of the human body as an antenna.It achieves high performance and stability through establish passive microstrip line and via models and active device-models.The proposed transceiver is designed and fabricated by FR4 printed cir-cuit board(PCB)process,the transceiver has the ability of configurable data rate up to 2 Mbps and it achieves-86 dBm receiving sensitivity at 2 Mbps data rate.Meanwhile,the transceiver out-put power dynamics range is 34 dB.Furthermore,with a visual interaction interface,the transceiv-er can be agility use in a variety of scenarios.Its measurements are verified on human body.The result shows that the transceiver has ability to send data from person to person by relying on hu-man body antenna radiation.The transceiver shows great prospect in wireless body area networks(WBAN)for telemedicine and emergency communication.

Selected Trace Elements in Domestic Water Boreholes and Their Implications on Human Health, in Huruma Estate, Eldoret Municipality, Uasin-Gishu County, Kenya

Trace elements constitute less than 1% of the rocks in the crust (Stumm and Morgan, 1991). In the human body, they constitute less than 100 mg/kg (0.01%). These elements are released to the environment naturally by weathering and volcanic activities (Flint and skinner, 1997). It has been observed that trace elements are greatly absorbed and retained in the body when in liquid diet. This phenomenon also influences the risk to human health, especially of infants and children whose immature digestive system further promote absorption of toxic heavy metals. The study was based on the analysis of domestic borehole water supplies in Huruma estate of Eldoret Municipality for selected trace elements and their implications on human health. The boreholes were systematically selected for sampling points and trace elements, Cr, Cu and Se analyzed using AAS. Statistical analysis for mean, standard deviation and confidence interval limits was done using SPSS. The statistical t-test was used to test for significance differences at (p = 0.05). The graphs were drawn using the Microsoft Excel package. The resulting data obtained from analysis were compared with WHO data for drinking water. In the study, the mean values of the following parameters were as follows: Chromium 17.9 μg/L, Copper 563 μg/L and Selenium 22.7 μg/L. There was a significant difference at 5% level of significance (p = 0.000) in all the parameter values among the sampling points in Huruma estate. The above mean values were far below the WHO recommended limits for drinking water. It was concluded that the borehole water from Huruma was fit for drinking and therefore could not cause cancer and cardiovascular diseases. However, borehole water should be used if other water sources (tap water etc.) were not available. All the industries near Huruma estate should carefully analyze and regularly monitor their liquid waste effluents to ensure that no harmful discharges get into the soil.

China's Innovation Research on International Communication of Human Rights Discourse on Ethnic Issues

The Western system of human rights discourse plays a leading role in the world.Western media have used the human rights discourse to attack China,especially on ethnic issues in China.The theory of transcultural political economy of communication is very inspiring to the innovation research on international communication of China's human rights discourse on ethnic issues.China's requires an innovation in the international communication of human rights discourse on ethnic issues so as to reconstruct the historical view of human rights discourse.In particular,the discourse resources of human rights protection in China should be explored from the social revolution in the 20 th century.Meanwhile,it is necessary to reconstruct the world discourse of human rights,that is,to construct the"human rights discourse of the Chinese nation,"and focus on human rights dialogue and cooperation with other civilizations and religious systems in the world.In particular,it is necessary to break through the"connection"or"integration"with the Western discourse,transcend the West-centered human rights discourse through competition and collision,reconstruct China's human rights discourse system,and improve the capability of international communication.

Origin of Fengshui Woods and Its Enlightenment to Modern Society

Origin of Fengshui Woods was studied, 3 representative standpoints (Fengshui doctrines, greening and worship of the god of land) about Fengshui woods were analyzed, and worship of the god of land was believed as a reasonable origin view through considering natural and social factors of ancient China, as well as universal mental characteristics of people in the prescience age. To identify advantages and disadvantages of these 3 views, Carl G. Jung' theory of mythological prototype criticism and Sir James George Frazer's anthropological study were borrowed to prove that Fengshui Woods originated from ancient Chinese worship of the god of land, and such worship gradually evolved into practices of planting Fengshui Woods for their propitious signs. On this basis, enlightenment of Fengshui Woods to modern society was disclosed to protect present Fengshui Woods and forest resources.

SF/HGF-c-Met autocrine and paracrine promote metastasis of hepatocellular carcinoma

AIM: To explore the role of SF/HGF-Met autocrine and paracrine in metastasis of hepatocellular carcinoma (HCC). METHODS: SF/HGF and c-met transcription and protein expression in HCC were examined by RT-PCR and Western Blot in 4 HCC cell lines, including HepG2, Hep3B, SMMC7721 and MHCC-1, the last cell line had a higher potential of metastasis. sf/hgf cDNA was transfected by the method of Lipofectin into SMMC7721. SF/HGF and c-met antibody were used to stimulate and block SF/HGF-c-met signal transduction. Cell morphology, mobility, and proliferation were respectively compared by microscopic observation, wound healing assay and cell growth curve. RESULTS: HCC malignancy appeared to be relative to its met-SF/HGF expression. In MHCC-1, c-met expression was much stronger than that in other cell lines with lower potential of metastasis and only SF/HGF autocrine existed in MHCC-1. After sf/hgf cDNA transfection or conditioned medium of MHCC-1 stimulation, SMMC7721 changed into elongated morphology, and the abilities of proliferation (P 【 0.05) and mobility increased. Such bio-activity could be blocked by c-met antibody (P 【 0.05). CONCLUSION: The system of SF/HGF-c-met autocrine and paracrine played an important role in development and metastasis potential of HCC. Inhibition of SF/HGF-c-met signal transduction system may reduce the growth and metastasis of HCC.

Correlation between expression of cadherin and gap junctional communication in human lung carcinoma cells

The correlation between expression of Ca 2+ dependent cell adhesion molecule, cadherin, and gap junctional intercellular communication (GJIC) in human lung carcinoma PG cell line and connexin43 (Cx43) cDNA transfected PG cell clones was investigated. Results from immunoblotting and immunofluorescent staining revealed that cultured normal human lung cells (RF) expressed N cadherin. However, the expression level of N cadherin in PG cells was very low in comparison with normal RF cells. The Cx43 transfected PG clones exhibited comparable levels of Cx43 protein, but varied in the level of N cadherin expression and in the function of GJIC as measured by scrape loading and dye transfer (SLDT) method. Positive correlation between N cadherin and GJIC was demonstrated. The in vitro and in vivo growth examination results suggest that N cadherin mediated cell cell adhesions and Cx43 functional expression, the GJIC, may work coordinately with each other in regulation of cell growth and differentiation. Deficiency in both GJIC and cell adhesion may be crucial for cell transformation.

Analysis of wave propagation on human body based on stratified media model

Human body communication(HBC) is a promising near-field communication(NFC) method emerging in recent years. But existing theoretical models of HBC are too simple to simulate the wave propagation on human body. In this work, in order to clarify the propagation mechanism of electromagnetic wave on human body, a surface waveguide HBC theoretical model based on stratified media cylinder is presented. A numerical model analyzed by finite element method(FEM) is used for comparing and validating the theoretical model. Finally, results of theoretical and numerical models from 80 MHz to 200 MHz agree fairly well, which means that theoretical model can characterize accurate propagation mechanism of HBC signal. Meanwhile, attenuation constants derived from two kinds of models are within the range from 1.64 to 3.37, so that HBC signal can propagate effectively on human body. The propagation mechanism derived from the theoretical model is useful to provide design information for the transmitter and the modeling of the propagation channel in HBC.

Low Power Single-Chip RF Transceiver for Human Body Cormunication

Human body communication is proposed as a promising body proximal comanunication tech- nology for body sensor networks. To achieve low power and slmll volume ill the sensor nodes, a Ra-dio Frequency （RF） application-specific integrated circuit transceiver tbr Human Body Commnunication （HBC） is presented and the characteristics of HBC are investigated. A high data rate On-Off Keying （OOK）/Frequency-Shift Keying （FSK） modulation protocol and an OOK/FSK delrodulator circuit are introduced in this paper, with a data-rate-to-carrier-frequency ratio up to 70%. A low noise amplifier is proposed to handle the dynamic range problem and improve the sensitivity of the receiver path. In addi-tion, a low power autonmatic-gain-control system is realized using a novel architecture, thereby render-ing the peak detector circuit and loop filter unneces-sary. Finally, the complete chip is fabricated. Simula-tion results suggest receiver sensitivity to be-75 dBm. The transceiver shows an overall power con-smxption of 32 mW when data rate is 5 Mbps, de-livering a P1dB output power of - 30 dBm.