Effects of daily nitrogen and phosphorus input on planktonic community metabolism in a semi-enclosed bay by mesocosm experiment

Planktonic metabolism plays an important role in affecting the energy transportation and carbon cycle of the marine ecosystem.However,its regulation mechanism remains unclear under the continuously exogenous nutrient inputs in nearshore waters.In this study,a mesocosm experiment was conducted in a semi-enclosed bay,the Daya Bay,to explore the responses of plankton metabolic balance and community structure to a concentration gradient of daily nitrogen and phosphorus inputs.The results showed that nutrient enrichments promoted phytoplankton biomass,total primary production,and community respiration,and the promoting effect enhanced alongwith the increase of nutrient concentration.However,the net community production fluctuated more violently between autotrophic and heterotrophic with the increase of nutrient inputs and tended to be more heterotrophic from the 5 th day to the 10 th day of the experiment.In addition,the daily flux of nitrogen and phosphorus,2μmol/(L·d)and 0.066μmol/(L·d),respectively,could be regarded as a potential threshold for ecosystem stability and health,since most of the ecological characteristics related to plankton structure and function have undergone significant changes when the nutrient level is higher than that.In the nearshore enclosed or semi-enclosed waters,nutrient from continuous terrigenous input is likely to be concentrated and exceed this level,indicating the ecological risks due to the metabolic unbalance and the deterioration of plankton community structure.

Microbial community structure and functional metabolic diversity are associated with organic carbon availability in an agricultural soil

Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertilizer with organic amendments incorporated（NPKM, NPKS） could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis（PCA） based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis（RDA） indicated that soil organic carbon（SOC） availability, especially soil microbial biomass carbon（Cmic） and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China＇s soil resource.

Probe into Reverse Operation of Apoptosis Gene

Objective:The programmed death process of cells according to gene coding belongs to apoptotic natural extinction(PCD).The purpose of this study is to explore the phenomenon of“returning to old age and rejuvenating children”in the extreme anoxia,no nutrients and survival in the extreme environment of fish and earthworm.Methods:the adult earthworms were put into the sealed quartz sand or fine yellow sand plastic bottle with humidity of 35-40%70 ml and poured out 100-150 d,then put back into the natural environment(simulated natural plastic basin)and raised 100-150 d,to collect the experimental information.The same object can be observed repeatedly.Results:The earthworms which were closed in the little oxygen-free and nutrition-deficient vials were reduced by autophagy,and the rings and reproductive pores disappeared completely.When they were put back into the natural environment for two or three months,they were all restored to their original morphological structure.Conclusion:Most of the same subjects underwent 1-3 years of cyclic observation.The biological structure was adapted to the changing environment.It was helped by the resonance of many biota and complex stress factors.