Development of the use of favin and nicotinamide-adenine nucleotide fluorescence in monitoringthe redox state of the free mitochondrial NADH/NAD+couple in cels,tissues and organs isreviewed.A break-through was the ide...Development of the use of favin and nicotinamide-adenine nucleotide fluorescence in monitoringthe redox state of the free mitochondrial NADH/NAD+couple in cels,tissues and organs isreviewed.A break-through was the identification of dihydrolipoamide dehydrogenase(FpL)asthe major NAD-linked fluorescent fla voprotein of mitochondria.This mitochondrial matrix fla-voprotein is in equilibriwn with the fre NADH/NAD+pool and its mid-potential is suficientlynear to that of NADH/NAD+so that its percentage reduction follows that of the latter.Pos.sibilities of monitoring mitochondial and cytosolic NADH depend on the population density ofmitochondria and thus are tissue-dependent.Upon a shift toward reduction,fluorescenceintensitis of NADH and flavins swing to reciprocal directions,so that the NADH/favin fluo-rescence ratio can be used to increase the sensitivity of redox monitoring.This method is attainingwidening use in studies on metabolic regulation under normal and pathological conditions.展开更多
文摘Development of the use of favin and nicotinamide-adenine nucleotide fluorescence in monitoringthe redox state of the free mitochondrial NADH/NAD+couple in cels,tissues and organs isreviewed.A break-through was the identification of dihydrolipoamide dehydrogenase(FpL)asthe major NAD-linked fluorescent fla voprotein of mitochondria.This mitochondrial matrix fla-voprotein is in equilibriwn with the fre NADH/NAD+pool and its mid-potential is suficientlynear to that of NADH/NAD+so that its percentage reduction follows that of the latter.Pos.sibilities of monitoring mitochondial and cytosolic NADH depend on the population density ofmitochondria and thus are tissue-dependent.Upon a shift toward reduction,fluorescenceintensitis of NADH and flavins swing to reciprocal directions,so that the NADH/favin fluo-rescence ratio can be used to increase the sensitivity of redox monitoring.This method is attainingwidening use in studies on metabolic regulation under normal and pathological conditions.
