Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological stu...Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological study of A.dispar in Mehran River,Hormuzgan Province,South of Iran,a total of 97 fish specimens were collected in 24 January 2006.Results: 4 specimens(4.12%) including 1 male and 3 female were found infected with C.complanalum metacercaria.These metacercaria were coiled in the epiderm on the body surface of infected fishes.The infection is known as yellow spot disease.The parasite abundance,intensity and prevalence were 0.03%,1.25%and 4.12%,respectively.The infection was higher in females than males.Conclusions:To the best of our knowledge,this is the first report on occurrence of C. complanatum metacercaria in A.dispar in Iran.展开更多
A novel Ci7N Lycopodium alkaloid(LA),lycoplanine B(1),containing an unusual formyl group,along with two new LAs,lycoplanines C(2)and D(3),were isolated from the whole plant of Lycopodium complanatum.Their structures w...A novel Ci7N Lycopodium alkaloid(LA),lycoplanine B(1),containing an unusual formyl group,along with two new LAs,lycoplanines C(2)and D(3),were isolated from the whole plant of Lycopodium complanatum.Their structures were elucidated by extensive NMR techniques,including 1D-and 2D-NMR experiments,as well as comparing their spectral data with those of the known analogues.A possible biogenetic pathway for 1 was also proposed.展开更多
Two rare lyconadin-type Lycopodium alkaloids,lyconadins G(1)and H(2),together with four known ones(3–6),were isolated from Lycopodium complanatum.The structures were determined on the basis of their spectroscopic ana...Two rare lyconadin-type Lycopodium alkaloids,lyconadins G(1)and H(2),together with four known ones(3–6),were isolated from Lycopodium complanatum.The structures were determined on the basis of their spectroscopic analyses,and the absolute configuration of 1 was established by an X-ray crystallographic analysis.It is the first time to establish the absolute configuration of lyconadin-type Lycopodium alkaloid by an X-ray diffraction experiment.In addition,these findings may provide more information for the biosynthesis of lyconadins.展开更多
Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laborator...Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.展开更多
In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore ...In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore the prevention technology of clinostomiasis caused by C.complanatum and promote development of heron artificial breeding technology,we observed epidemic characteristics and clinical symptom of clinostomiasis,and carried out pathological examination and drug treatment trials.The results showed that 20 mg/kg albendaszole had a distinctive effect on clinostomiasis.The comprehensive prevention and control technology against clinostomiasis was further put forward.展开更多
文摘Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological study of A.dispar in Mehran River,Hormuzgan Province,South of Iran,a total of 97 fish specimens were collected in 24 January 2006.Results: 4 specimens(4.12%) including 1 male and 3 female were found infected with C.complanalum metacercaria.These metacercaria were coiled in the epiderm on the body surface of infected fishes.The infection is known as yellow spot disease.The parasite abundance,intensity and prevalence were 0.03%,1.25%and 4.12%,respectively.The infection was higher in females than males.Conclusions:To the best of our knowledge,this is the first report on occurrence of C. complanatum metacercaria in A.dispar in Iran.
基金supported by the NSFC-Joint Foundation of Yunnan Province(No.U1502223)the National Natural Science Foundation of China(Nos.81773611 and 21602227)the Science and Technology Program of Yunnan province(Nos.2016FB140 and 2015FB173).
文摘A novel Ci7N Lycopodium alkaloid(LA),lycoplanine B(1),containing an unusual formyl group,along with two new LAs,lycoplanines C(2)and D(3),were isolated from the whole plant of Lycopodium complanatum.Their structures were elucidated by extensive NMR techniques,including 1D-and 2D-NMR experiments,as well as comparing their spectral data with those of the known analogues.A possible biogenetic pathway for 1 was also proposed.
基金This work was financially supported by the NSFC-Joint Foundation of Yunnan Province(No.U1502223)the National Natural Science Foundation of China(No.21402212)+1 种基金the Science and Technology Program of Yunnan Province(No.2015FB173)the CAS“Light of West China”Program and Youth Innovation Promotion Association CAS(X.-D.Wu).
文摘Two rare lyconadin-type Lycopodium alkaloids,lyconadins G(1)and H(2),together with four known ones(3–6),were isolated from Lycopodium complanatum.The structures were determined on the basis of their spectroscopic analyses,and the absolute configuration of 1 was established by an X-ray crystallographic analysis.It is the first time to establish the absolute configuration of lyconadin-type Lycopodium alkaloid by an X-ray diffraction experiment.In addition,these findings may provide more information for the biosynthesis of lyconadins.
基金financially supported by Aligarh Muslim University(grant No.09PHDL128)
文摘Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.
基金Supported by National Spark Program(2011GA780060)
文摘In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore the prevention technology of clinostomiasis caused by C.complanatum and promote development of heron artificial breeding technology,we observed epidemic characteristics and clinical symptom of clinostomiasis,and carried out pathological examination and drug treatment trials.The results showed that 20 mg/kg albendaszole had a distinctive effect on clinostomiasis.The comprehensive prevention and control technology against clinostomiasis was further put forward.
基金The project (P-06-04) of State Key Laboratory of Phytochemistry and Plant Resourcesin West China,Kunming Institute of Bota-ny,Chinese Academy of Sciencesthe 973 Program(No.2009CB522300)
