Objective: To explore the possible IgG1 binding receptors by protein-protein docking experiments. Methods: The protein-protein cognate interactions such as IgG with Fc Receptors (FcRs) potentiate signaling cascades to...Objective: To explore the possible IgG1 binding receptors by protein-protein docking experiments. Methods: The protein-protein cognate interactions such as IgG with Fc Receptors (FcRs) potentiate signaling cascades to ameliorate antigen uptake, processing and presentation are studied by protein-protein docking experiments. Results: However, the propensity of IgG interactions with other cognate receptors largely remains obscure. In this direction, possibilities of IgG1 binding with various five receptors were explored. In this study, we report previously unidentified associations between IgG1 and other receptors. Herein, we show that IgG1 binds to the granulocyte-macrophage receptor, β common receptor and complementaryreceptor(complementary receptor I and complementary receptor II) to form a complex structure. We show the binding ability and important protein-protein interactions of IgG1 with four receptors in comparison to Fc Receptor, and also find out the conserved amino acids and hydrophobic-hydrophobic interactions amongst them. Conclusions: Comparative interaction studies of IgG1 binding to various receptors revealed close similarities of IgG1 binding to its native receptor Fc. In conclusion, our study has shown the comparable binding efficiency of four receptors to IgG1 apart from the conventional Fc receptor.展开更多
Objective To contrast the roles of Asparagus officinalis polysaccharide on erythrocyte of S180 mice played in immunological reaction of lymphocytes.To study the effect of Asparagus officinalis polysaccharide on the er...Objective To contrast the roles of Asparagus officinalis polysaccharide on erythrocyte of S180 mice played in immunological reaction of lymphocytes.To study the effect of Asparagus officinalis polysaccharide on the erythrocyte function of S180 mice.Methods Suspensions of lymphocytes(1×106/mL)and autologous plasma were respectively separated from anticoaguted whole blood of healthy mice with the lymphocyte separation medium.The erythrocytes(1×108/mL)were separated from whole blood of Asparagus officinalis polysaccharide mice.Using the autologous plasm as reactive medium,the role of erythrocytes in regulating the immunological reaction of lymphocytes was appraised.The expression of CD25 on lymphocytes was detected using flow cytometry.Results The immunogical regulating ability of erythrocyte in mice with control groups is much lower than that of normal groups,and the immunogical regulating ability of erythrocyte in mice with Asparagus officinalis polysaccharide groups is much higher than that of control groups.Conclusions According to the effects of erythrocyte CD35 on the immuno-response of lymphocyte and the different of the expression of CD25 on lymphocytes,we prove that Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice.展开更多
Objective To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1 in S180 mice.Methods Red blood cells from mice venous blood were labeled by rat ant...Objective To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1 in S180 mice.Methods Red blood cells from mice venous blood were labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody.Using flow cytometry,we determined the number of ECR1.Using microscope,we studied the adherence between erythrocyte immunity and C3b receptor or tumor-cell by RBC-C3bRR and DTER.Results Comparing the mean value of the number of CR1 on each RBC of high and middle groups with control groups,the mean value of the number of CR1,RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups are increased significantly.Conclusions Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice,which may be one of its most important antitumor mechanisms.展开更多
The study was carried out to observe the effects of heparin,dexamethasone and ibuprofen on the clearance function of the Kupffer cell complement receptors(KCCR) in experimental acute obstructive cholangitis (AOC) in r...The study was carried out to observe the effects of heparin,dexamethasone and ibuprofen on the clearance function of the Kupffer cell complement receptors(KCCR) in experimental acute obstructive cholangitis (AOC) in rats.It was found that in AOC,the mortality rate was far higher in not treated group and dexamethasone treated group than in ibuprofen and heparin treated groups and the clearance function of KCCR was significantly decreased 48h after the onset of AOC but it was strengthened with the administration of heparin and ibuprofen.The prominent pathological changes of AOC were the damages on the hepatocytes,degeneration of Kupffer cells with reduction of their functions and damages of the pulmonary alveolar walls. The phagocytic function of Kupffer cells were restored and damages of the liver and lungs abated after the administration of heparin.This study was financially suppoited by the National Funds for Natural Science of China (Approval certificate numberof NSFC 38870770).展开更多
Objective To document the possible effect of tyrosine kinase autophosphorylation on erythrocyte insulin-receptor after 12 weeks of metformin administration from the women with polycystic ovary syndrome （PCOS）. Metho...Objective To document the possible effect of tyrosine kinase autophosphorylation on erythrocyte insulin-receptor after 12 weeks of metformin administration from the women with polycystic ovary syndrome （PCOS）. Methods Thirty non-obese （BMI〈25 kg/m2） healthy women with normal reproductive cycles were evaluated by conventional criteria as the control 30 non-obese women with PCOS were categorized as nob-PCOS group, 40 obese women with PCOS （BMI 25 kg/m2） were categorized as ob-PCOS group. Subjects with PCOS were given metformin 850 mg/d for 12 weeks. The autophosphorylation of insulin receptor p Yl158, pyl162/1163 and insulin receptor-fl subunit （IR-~） from the solubilised erythrocyte were detected by ELISA. Results 1） Only the autophosphorylation level of p Yl l 58 in nob-PCOS and ob-PCOS groups was lower （P〈O.05） than that in control group and it can be increased after insulin stimulation （P〈O.05）; no similar changes were found in pYl162/1163 （P〉O.05）, even insulin-stimulated insulin receptor （P〉O.05） from both of PCOS groups. 2） After metformin administration, the p Y1158, p Y1162/p Y1163 autophosphorylation levels were increased （P〈O.O1） and further increased after plus insulin stimulation in vitro （P〈0. 01）. The p Y1162/1163 autophosphorylation level was increased in nob-PCOS group （P〈0.01） and ob-PCOS group （P〈0.05）. 3） No difference was found in concentration of IR-B between control group and both of PCOS groups following metformin administration （P〉0.05）. In summary, oral administration of metformin led to an significant increase in tyrosine kinase activity in both groups of PCOS. Conclusion The impairment of tyrosine autophosphorylation at pYl158 may contribute to insulin resistance, the tyrosine kinase activity per receptor of solubilised erythrocytes were significantly increased by metformin administration and the effect of metformin on insulin-receptor tyrosine kinase activity appeared to be independent of either of these variables.展开更多
文摘Objective: To explore the possible IgG1 binding receptors by protein-protein docking experiments. Methods: The protein-protein cognate interactions such as IgG with Fc Receptors (FcRs) potentiate signaling cascades to ameliorate antigen uptake, processing and presentation are studied by protein-protein docking experiments. Results: However, the propensity of IgG interactions with other cognate receptors largely remains obscure. In this direction, possibilities of IgG1 binding with various five receptors were explored. In this study, we report previously unidentified associations between IgG1 and other receptors. Herein, we show that IgG1 binds to the granulocyte-macrophage receptor, β common receptor and complementaryreceptor(complementary receptor I and complementary receptor II) to form a complex structure. We show the binding ability and important protein-protein interactions of IgG1 with four receptors in comparison to Fc Receptor, and also find out the conserved amino acids and hydrophobic-hydrophobic interactions amongst them. Conclusions: Comparative interaction studies of IgG1 binding to various receptors revealed close similarities of IgG1 binding to its native receptor Fc. In conclusion, our study has shown the comparable binding efficiency of four receptors to IgG1 apart from the conventional Fc receptor.
文摘Objective To contrast the roles of Asparagus officinalis polysaccharide on erythrocyte of S180 mice played in immunological reaction of lymphocytes.To study the effect of Asparagus officinalis polysaccharide on the erythrocyte function of S180 mice.Methods Suspensions of lymphocytes(1×106/mL)and autologous plasma were respectively separated from anticoaguted whole blood of healthy mice with the lymphocyte separation medium.The erythrocytes(1×108/mL)were separated from whole blood of Asparagus officinalis polysaccharide mice.Using the autologous plasm as reactive medium,the role of erythrocytes in regulating the immunological reaction of lymphocytes was appraised.The expression of CD25 on lymphocytes was detected using flow cytometry.Results The immunogical regulating ability of erythrocyte in mice with control groups is much lower than that of normal groups,and the immunogical regulating ability of erythrocyte in mice with Asparagus officinalis polysaccharide groups is much higher than that of control groups.Conclusions According to the effects of erythrocyte CD35 on the immuno-response of lymphocyte and the different of the expression of CD25 on lymphocytes,we prove that Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice.
文摘Objective To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1 in S180 mice.Methods Red blood cells from mice venous blood were labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody.Using flow cytometry,we determined the number of ECR1.Using microscope,we studied the adherence between erythrocyte immunity and C3b receptor or tumor-cell by RBC-C3bRR and DTER.Results Comparing the mean value of the number of CR1 on each RBC of high and middle groups with control groups,the mean value of the number of CR1,RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups are increased significantly.Conclusions Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice,which may be one of its most important antitumor mechanisms.
文摘The study was carried out to observe the effects of heparin,dexamethasone and ibuprofen on the clearance function of the Kupffer cell complement receptors(KCCR) in experimental acute obstructive cholangitis (AOC) in rats.It was found that in AOC,the mortality rate was far higher in not treated group and dexamethasone treated group than in ibuprofen and heparin treated groups and the clearance function of KCCR was significantly decreased 48h after the onset of AOC but it was strengthened with the administration of heparin and ibuprofen.The prominent pathological changes of AOC were the damages on the hepatocytes,degeneration of Kupffer cells with reduction of their functions and damages of the pulmonary alveolar walls. The phagocytic function of Kupffer cells were restored and damages of the liver and lungs abated after the administration of heparin.This study was financially suppoited by the National Funds for Natural Science of China (Approval certificate numberof NSFC 38870770).
基金supported by the Projects in the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (Grant No. 2007BAI04B04)the National Natural Science Foundation of China(Grant No 30672223)
文摘Objective To document the possible effect of tyrosine kinase autophosphorylation on erythrocyte insulin-receptor after 12 weeks of metformin administration from the women with polycystic ovary syndrome （PCOS）. Methods Thirty non-obese （BMI〈25 kg/m2） healthy women with normal reproductive cycles were evaluated by conventional criteria as the control 30 non-obese women with PCOS were categorized as nob-PCOS group, 40 obese women with PCOS （BMI 25 kg/m2） were categorized as ob-PCOS group. Subjects with PCOS were given metformin 850 mg/d for 12 weeks. The autophosphorylation of insulin receptor p Yl158, pyl162/1163 and insulin receptor-fl subunit （IR-~） from the solubilised erythrocyte were detected by ELISA. Results 1） Only the autophosphorylation level of p Yl l 58 in nob-PCOS and ob-PCOS groups was lower （P〈O.05） than that in control group and it can be increased after insulin stimulation （P〈O.05）; no similar changes were found in pYl162/1163 （P〉O.05）, even insulin-stimulated insulin receptor （P〉O.05） from both of PCOS groups. 2） After metformin administration, the p Y1158, p Y1162/p Y1163 autophosphorylation levels were increased （P〈O.O1） and further increased after plus insulin stimulation in vitro （P〈0. 01）. The p Y1162/1163 autophosphorylation level was increased in nob-PCOS group （P〈0.01） and ob-PCOS group （P〈0.05）. 3） No difference was found in concentration of IR-B between control group and both of PCOS groups following metformin administration （P〉0.05）. In summary, oral administration of metformin led to an significant increase in tyrosine kinase activity in both groups of PCOS. Conclusion The impairment of tyrosine autophosphorylation at pYl158 may contribute to insulin resistance, the tyrosine kinase activity per receptor of solubilised erythrocytes were significantly increased by metformin administration and the effect of metformin on insulin-receptor tyrosine kinase activity appeared to be independent of either of these variables.
