Urban development should rely on the form of architecture,and incorporating urban planning concepts into architectural design can better assist urban construction.Taking the design of Incheon Geomdan Cultural Complex ...Urban development should rely on the form of architecture,and incorporating urban planning concepts into architectural design can better assist urban construction.Taking the design of Incheon Geomdan Cultural Complex in South Korea as an example,this paper analyzes how to reflect urban planning elements in architectural design,and studies the role and value of cultural complex architecture in urban development from multiple perspectives such as culture,ecological environment,and public space.It hopes to gain more research and design ideas to promote the development of cultural industries in the region,leverage the advantages of regional cultural formats,and create new cultural settlements in cities.展开更多
Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activit...Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.展开更多
Being as one figurative form of language,metaphor plays the most complicated role to make language colorful and vivid.Demonstrating the types and the features of metaphor,this article will focus on the point that meta...Being as one figurative form of language,metaphor plays the most complicated role to make language colorful and vivid.Demonstrating the types and the features of metaphor,this article will focus on the point that metaphor is a complex language phenomenon heavily loaded with the factor of culture.展开更多
The cultural tourism complex is a comprehensive development model with cultural soft power as its core competitiveness, and is an industrial development system with tourism as the forerunner, culture as the core, and ...The cultural tourism complex is a comprehensive development model with cultural soft power as its core competitiveness, and is an industrial development system with tourism as the forerunner, culture as the core, and real estate as the platform and consumption carrier. The development status and trend of the cultural tourism complex as a new format of tourism industry integration under the guidance of the national strategy of all-for-one tourism are worthy of attention and discussion. With Xishuangbanna's two cultural tourism complexes of Gaozhuangxishuangjing and Xishuangbanna-based Wanda International Resort as the research object, this paper compared the similarities and differences of the two tourism complexes in their development and their respective problems, and analyzed the basis and condition for their co-opetition development. The ultimate goal of this paper was to seek the relevant countermeasures and suggestions for the two cultural tourism complexes to complement each other, promote each other, and jointly promote the development of tourism in Xishuangbanna.展开更多
Nowadays,the American citizens own the most quantity of guns in the world.This paper focuses on American's complex towards guns,and analyzes this issue from four aspects:history,laws,economy and culture.
Synaptonemal complex (SC for short) spreading in plant is a new technique devel-oped in recent years. It has advantages of fast observation with a light microscope andhigh resolution with an electron microscope, as We...Synaptonemal complex (SC for short) spreading in plant is a new technique devel-oped in recent years. It has advantages of fast observation with a light microscope andhigh resolution with an electron microscope, as Well as the capability of analysingchromosome pairing of zygotene nuclei. This technique has been widely applied not展开更多
基金Sponsored by Beijing Natural Science Foundation General Project(8212009)Organized Research Project of North China University of Technology in 2023(110051360023XN278).
文摘Urban development should rely on the form of architecture,and incorporating urban planning concepts into architectural design can better assist urban construction.Taking the design of Incheon Geomdan Cultural Complex in South Korea as an example,this paper analyzes how to reflect urban planning elements in architectural design,and studies the role and value of cultural complex architecture in urban development from multiple perspectives such as culture,ecological environment,and public space.It hopes to gain more research and design ideas to promote the development of cultural industries in the region,leverage the advantages of regional cultural formats,and create new cultural settlements in cities.
文摘Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.
文摘Being as one figurative form of language,metaphor plays the most complicated role to make language colorful and vivid.Demonstrating the types and the features of metaphor,this article will focus on the point that metaphor is a complex language phenomenon heavily loaded with the factor of culture.
文摘The cultural tourism complex is a comprehensive development model with cultural soft power as its core competitiveness, and is an industrial development system with tourism as the forerunner, culture as the core, and real estate as the platform and consumption carrier. The development status and trend of the cultural tourism complex as a new format of tourism industry integration under the guidance of the national strategy of all-for-one tourism are worthy of attention and discussion. With Xishuangbanna's two cultural tourism complexes of Gaozhuangxishuangjing and Xishuangbanna-based Wanda International Resort as the research object, this paper compared the similarities and differences of the two tourism complexes in their development and their respective problems, and analyzed the basis and condition for their co-opetition development. The ultimate goal of this paper was to seek the relevant countermeasures and suggestions for the two cultural tourism complexes to complement each other, promote each other, and jointly promote the development of tourism in Xishuangbanna.
文摘Nowadays,the American citizens own the most quantity of guns in the world.This paper focuses on American's complex towards guns,and analyzes this issue from four aspects:history,laws,economy and culture.
基金Project supported by the Zhejiang Provincial Natural Foundation of China.
文摘Synaptonemal complex (SC for short) spreading in plant is a new technique devel-oped in recent years. It has advantages of fast observation with a light microscope andhigh resolution with an electron microscope, as Well as the capability of analysingchromosome pairing of zygotene nuclei. This technique has been widely applied not
