Effects of Compound Salviae Miltiorrhizae Injection on Metabolism of Phosphoinosititides in Myocardial Ischemia

Using the model of acute myocardial ischemia in rats formed by ligation of the descending an-terior branch of the left coronary artery, this study showed some effects of compound Salviae miltiorrhizae in-jection ( CSMI) on metabolism of phosphoinositide in myocardial ischemia. The results of the experimentshowed that after 10 minutes of acute ischemia, the function of myocardial messangers ot phosphoinositide in-creased, and the levels of phosphatidylinositol-4, 5-biphosphate ( PIP2 ) and inositol-1, 4, 5-trisphosphate(IP3 ) were obviously higher than those of the non-ischemia control groups ( P < 0. 01 , n = 7) . CSMI (2 glkgbody weight) could markediy inhibit myocardial PIP2 and IP3, whose levels became lower than those of theischemia control groups ( P < 0 . 01 , n = 7 ) .

Clinical Study of Compound Radix Salvia Miltiorrhizae for Severe Pancreatitis

Compound Radix Salvia Miltiorrhizae injection was administered after operation for 28 casesof severe pancreatitis, and 13 cases were taken as a control group. The results showed that : (1) The differ-ence was not obvious in the morbidity of complications between the two groups, but the mortality (3.6% ) ofthe trial group was significantly lower tlian that ( 30. 8% ) of the control group ( P < 0. 05 ) ; ( 2 ) Hematocritwas clearly decreased from 46. 1 5. 2% to 33. 2 3. 9% in the trial one ( P < 0. 05) , but platelet andhemoglobin showed no statistical signiticance. It is concluded that compound Salvia miltiorrhizae inlectionmight improve hemorheologic abnormalities of the disease, promote the recovery of the pancreatic tissue,and correct the serious complications such as adult respiratory distress syndrome etc.

TREATMENT OF 40 CASES OF CHRONIC ECZEMA WITH POINT-INJECTION OF COMPOUND RADIX SALVIAE MILTIORRHIZAE INJECTION

The authors have used point-injection of compound Radix Salviae Miltiorrhizae Injection to treat 40 cases of chronic eczema and achieved a satisfactory therapeutic effect. The total effective rate was 95%.

Effect of Danshen on apoptosis and NF-кB protein expression of the intestinal mucosa of rats with severe acute pancreatitis or obstructive jaundice

BACKGROUND:Intestinal mucosa injury in cases of severe acute pancreatitis(SAP) or obstructive jaundice(OJ) is one of the main reasons for the accelerated aggravation of these diseases.Besides being an organ to digest and absorb nutrients,the intestine is also a unique immune organ.When SAP and OJ develop,the destruction of the intestinal mucosa barrier is an important contributing factor for the development of bacterial translocation,systemic inflammatory response syndrome,and multiple organ dysfunction syndrome.It is important to protect the intestinal mucosa in the therapy for SAP and OJ.In this study,we determined the effect of Radix Salviae Miltiorrhizae(Danshen) injection on apoptosis and NF-κB P65 protein expression in the intestinal mucosa of rats with SAP or OJ,and explored the protective mechanism of Danshen in their mucosa.METHODS:Sprague-Dawley rats were used in the SAP and OJ experiments.These rats were randomly divided into shamoperated,model control,and treated groups.At various times after operation,the mortality rates were calculated.Subsequently,the rats were killed to assess the pathological changes,the expression levels of Bax and NF-κB proteins,and the apoptosis indices in the intestinal mucosa.RESULTS:Compared to the corresponding model control group,the number of SAP or OJ rats that died in the treated group decreased but showed no statistically significant difference.At all time points after operation,there was no significant difference between the treated and model control groups in the staining intensity as well as the product of staining intensity and positive staining rate of Bax protein in the intestinal mucosa of SAP and OJ rats.At 3 hours after operation,the apoptosis index of the intestinal mucosa of SAP rats in the treated group was lower than that in the model control group(P【0.01).At 12 hours after operation in SAP rats and 28 days after operation in OJ rats,the staining intensity as well as the product of staining intensity and positive staining rate of NF-κB protein of the intestinal mucosa in the treated group were lower than those in the model control group(P【0.01).CONCLUSION:Danshen exerts protective effects on the intestinal mucosa of SAP and OJ rats perhaps by inhibiting apoptosis and down-regulating NF-κB protein.

Microwave digestion polarography for determining seven trace elements in Salvia Miltiorrhiza Root and compound Salvia Miltiorrhiza Root injection simultaneously

The sensitive second derivative waves of Cu(Ⅱ),Pb(Ⅱ),Cd(Ⅱ),Ni(Ⅱ),Zn(Ⅱ),Fe(Ⅱ)and Mn(Ⅱ),forming in the substrate solution(pH=9.26)consisting of ammonia,ammonium chlorid,gelatin and ascorbic acid were researched.The peak potentials of the polarographic waves of the seven ions are at about 0.48,0.63,0.79,1.04,1.28,1.44 and 1.60 V respectively.Combining with microwave technique,a new method for the simultaneous determination of the seven trace elements in Chinese traditional medicine was developed.The method is easy to operate,rapid,simple and convenient.When the signal-to-noise rate equals 3,the detection limits of Cu,Pb,Cd,Ni,Zn,Fe and Mn are 4.2×10 3,5.3×10 3,2.1×10 3,5.8×10 4,3.0×10 3,7.7×10 4 and 1.1×10 3 μg/mL respectively.Well linear relationships exist between the concentrations and the peak currents when Cu,Pb,Cd,Ni,Zn,Fe and Mn concentrations are within 8.5×10 3 10,9.7×10 3 10,4.5×10 3 10,1.2×10 3 10,6.4×10 3 10,1.5×10 3 10 and 2.8×10 3 10 μg/mL,respectively.The method has been used to the simultaneous determination of the seven trace elements in Salvia Miltiorrhiza Root and compound Salvia Miltiorrhiza Root injection,the relative standard deviations(RSDs)of the Cu,Pb,Cd,Ni,Zn,Fe and Mn in the two medicines are 3.9% and 5.8%,4.0% and 4.1%,4.3% and 5.7%,4.9% and 5.3%,4.4% and 4.7%,3.5% and 4.0%,0.51% and 2.8%,respectively;the comparisons of the determination results with the values obtained by the standard method indicate that the presented method has very well veracity.

Effect of Salvia Miltiorrhiza on Expression of the MMP-2 9 in Tissue of Early Stage Acute Lung Injury in Rats with Severe Acute Pancreatitis

Objective:To investigate the effect of salvia miltiorrhiza on expression of the MMP-2、9 and TIMP-1、TIMP-2 in tissue of acute lung injury of severe acute pancreatitis(SAP).Methods:MMP-2、9 expression and changes of the lung were measured after the SAP rats were induced by retrograde injection of 5%sodium tauocholate into hepatopancreatic duct.The changes of those parameters were also measured after salvia miltiorrhiza was injected intramuscularly just after induction of SAP.Results:The level of MMP-2、9 in pancreas and lung in SAP group were significantly higher than those in sham;The level of MMP-2、9 in salvia miltiorrhiza group were significantly lower than those in SAP group. Conclusion:MMP-2、9 were overexpressed in Acute lung injury (ALI) induced by SAP, salvia miltiorrhiza downregulates MMP-2、9 expression and decreased injury of lung tissue.

复方丹参注射液治疗重症胰腺炎的临床研究

手术治疗重症胰腺炎后，应用复方丹参注射液治疗２８例，对照组１３例。结果表明：（１）两组并发症的发生率无明显差异，治疗组病死率３．６％，对照组病死率３０．８％，其差异显著（Ｐ＜０．０５）。（２）治疗组的红细胞压积从术前的４６．１±５．２％降低到３３．２±３．９％，其差异显著（Ｐ＜０．０５），而血小板和血红蛋白的变化无统计学意义。认为：复方丹参注射液能够改善重症胰腺炎的血液流变学异常，有助于胰腺组织的康复和成人呼吸窘迫综合征等严重并发症的纠正。

生长抑素联用丹参或乌司他丁治疗重症急性胰腺炎疗效观察

目的探讨生长抑素联用丹参或乌司他丁治疗重症急性胰腺炎(SAP)的效果。方法将246例SAP患者分成4组:生长抑素组(A组);生长抑素联合丹参组(B组);生长抑素联合乌司他丁组(C组);生长抑素联合乌司他丁和丹参组(D组)。观察4组患者腹痛及腹胀症状消失时间、心率及呼吸恢复正常时间、血淀粉酶及血糖恢复正常时间、住院时间、总住院费用及有效率。结果患者腹痛及腹胀消失时间、心率及呼吸恢复正常时间、血淀粉酶及血糖恢复正常时间和住院时间最短为D组,其他依次为C、B、A组,4组比较差异有统计学意义(P<0.05);A、B、C、D组总有效率分别为65.57%、77.42%、81.54%、89.66%,4组比较差异有统计学意义(P<0.05);4组不良反应发生率比较,差异无统计学意义(P>0.05)。结论生长抑素能有效治疗SAP,与乌司他丁或丹参联用能提高疗效,但乌司他丁的疗效优于丹参,乌司他丁与丹参联用能进一步提高疗效。

生长抑素联合复方丹参注射液治疗急性胰腺炎临床研究

目的:观察生长抑素联合复方丹参注射液治疗急性胰腺炎(AP)的临床疗效。方法:采用随机对照分组的临床研究方法,将2002年6月—2006年12月收治的62例AP患者分为对照组和观察组,每组31例。对照组在常规禁食、胃肠减压等治疗的基础上,先静脉注射生长抑素250μg,然后改用3 000μg加生理盐水500 ml以250μg/h的速度持续静脉滴注(静滴),连续7～14 d;观察组在对照组治疗基础上,予复方丹参注射液20 ml溶于生理盐水250 ml中静滴,每日1次,连续7～14 d。观察并比较两组患者的症状,腹部体征消失时间,血、尿淀粉酶,肝功能恢复正常时间,血钙浓度变化和多器官功能障碍综合征(MODS)发生率、病死率。结果:观察组治疗后血淀粉酶显著低于对照组,而血钙水平显著高于对照组,尤以治疗后3 d和5 d更为明显(P均<0.01)。观察组痊愈率(38.7%)和总有效率(90.4%)均明显高于对照组(16.2%和77.4%,P<0.01和P<0.05),而MODS的发生率(19.3%)和病死率(6.5%)均显著低于对照组(45.1%和16.2%,P均<0.05)。结论:生长抑素联合复方丹参注射液治疗AP疗效好于单纯生长抑素治疗。

中西医结合治疗重症急性胰腺炎(附30例报告)

目的 :探索中西医结合治疗重症急性胰腺炎 (Severe Acute Pancreatitis SAP)的方法。方法 :基本支持治疗加川芎嗪注射液 10～ 12 ml(2 ml:40 mg)、复方丹参注射液 2 0～ 30 ml(1m l:1g)、甲氰咪胍注射液 0 .8～ 1.2 g、5 - Fu0 .2 5～ 0 .5 g,静滴 ,每日 1次 ,平均疗程 12天。结果 :30例中 ,治愈 2 8例 ,死亡 2例 ,病死率 6 .6 7% (2 / 30 ) ;死亡原因为休克、ARDS、MOF,平均住院日 18天。随访 2 8例 ,平均随访时间 1.6年 ,无明显后遗症。结论 :本法能提高 SAP的治愈率 ,降低死亡率 ,减少并发症的发生率 ,医疗费用较低 ,使用方便。

丹参对重症急性胰腺炎内皮素-1 mRNA的影响

目的:探讨内皮素-1(ET-1)在重症急性胰腺炎(severeacutepancreatitis,SAP)胰腺中的表达对胰腺损伤的作用机制及丹参注射液对ET-1mRNA表达的影响.方法:Wistar大鼠45只,随机等分为模型组、药物组和对照组.以50g/L牛磺胆酸钠1mL/kg经胰管内逆行注射复制SAP模型.药物组在制模后im丹参[2mL/(kg·d)],1次/6h.对照组仅行假手术.各组动物在术后24h测血淀粉酶、ET-1和腹水量.使用原位杂交和图象分析检测胰腺ET-1mRNA的表达强度,并观察胰腺的病理变化.结果:模型组血中淀粉酶和腹水量分别为35.9±5.93μkat/L和9.87±2.34mL,显著高于药物组的23.96±5.56μkat/L和5.27±2.81mL及对照组的3.60±0.62μkat/L和0mL(P<0.001).模型组血中ET-1为185.47±20.80ng/L,高于药物组的164.27±18.53ng/L和对照组的72.90±17.27ng/L(P<0.05,P<0.001).模型组和药物组胰腺ET-1mRNA表达均增高,模型组显著高于药物组(28.22±1.15vs23.81±1.04,P<0.001).模型组和药物组胰腺均有病理改变,但药物组较模型组减轻(评分:10.45±1.42vs12.05±1.28,P<0.05).结论:SAP时胰腺ET-1mRNA高表达导致ET-1过度生成并与胰腺损伤有关.丹参能抑制胰腺ET-1mRNA的过度表达,从而对胰腺起保护作用.

重症急性胰腺炎大鼠肺脏iNOS mRNA表达及丹参的干预效应

目的探讨重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肺脏诱导型一氧化氮合酶(iNOS)mRNA的表达及其与肺组织损伤的关系和丹参的治疗效应。方法45只W istar大鼠随机分为3组。SAP模型组、丹参组均以5%牛磺胆酸钠经胰管内逆行注射(1 m l/kg)复制SAP模型,丹参组则在制模后立即肌肉注射丹参(2 m l.kg-1.d-1),每6 h 1次,共给药4次。对照组仅行假手术。各组动物在术后24 h测血一氧化氮(NO)、淀粉酶(AMY)和腹水量。使用原位杂交和图像分析检测肺iNOS mRNA的表达强度,并观察其病理变化。结果AP模型组血中NO、AMY、腹水量和肺脏iNOS mRNA表达显著高于丹参组和对照组(P<0.05,P<0.01);丹参组病理改变较模型组减轻。结论SAP时肺组织iNOS mRNA高表达导致NO过度生成并与肺脏损伤有关;丹参能抑制肺组织iNOS mRNA的过度表达,从而对肺组织起保护作用。

复方丹参注射液对人羊膜上皮细胞水通道蛋白3表达的影响

目的研究复方丹参注射液对人羊膜上皮细胞(human amniotic epithelial cells,hAECs)水通道蛋白3(aquaporin 3,AQP3)表达的影响,探讨复方丹参注射液治疗羊水量过少的机制。方法选取无并发症的8例羊水过少和8名羊水量正常的足月产妇的hAECs进行原代培养,采用RT-PCR和免疫印迹Western blot技术,检测复方丹参注射液不同浓度(0.000、0.001、0.010、0.020、0.060、0.100mg/mL)、不同作用时间(0、6、12、24、48h)作用前后,两组hAECs中AQP3 mRNA和蛋白的表达。结果 (1)羊水过少组hAECs中AQP3的表达较羊水正常组下调(P<0.05)。(2)复方丹参注射液浓度为0.010mg/mL时,两组hAECs中AQP3的表达均达到峰值,与其他浓度作用结果比较,差异有统计学意义(P<0.05)。(3)0.010mg/mL浓度的复方丹参注射液作用12h,两组hAECs中AQP3的表达均达到峰值,与其他作用时间比较,差异有统计学意义(P<0.05)。(4)0.010mg/mL浓度的复方丹参注射液作用12h后,两组hAECs中AQP3的表达均上调,但羊水过少组中AQP3的表达上调较羊水正常组明显(P<0.05)。结论复方丹参注射液可调节hAECs中AQP3的表达,羊水过少时复方丹参注射液对hAECs中AQP3表达的调节作用更加明显。

复方丹参注射液抗脂多糖诱导的兔弥漫性血管内凝血

目的:研究复方丹参注射液对脂多糖(LPS)诱导的兔弥漫性血管内凝血(DIC)的作用。方法:用LPS诱导兔DIC模型。凝固法测定纤维蛋白原含量,全自动凝血分析仪检测活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和纤维蛋白原含量;全自动血细胞分析仪进行血小板计数;全自动血浆分析仪测定谷丙转氨酶(ALT)以及血尿素氮(BUN);发色底物法测定蛋白C及抗凝血酶Ⅲ的活性。观察复方丹参注射液对LPS诱导的兔DIC的拮抗作用。结果:兔耳缘静脉持续滴注LPS,观察到:APTT和PT显著延长;血小板计数和纤维蛋白原含量明显减少;ALT和BUN显著升高;蛋白C和抗凝血酶Ⅲ的活性明显降低。给予复方丹参注射液后,APTT和PT的延长明显缩短;血小板计数和纤维蛋白原的含量均明显恢复;ALT和BUN显著下降;蛋白C及抗凝血酶Ⅲ的活性显著改善。结论:复方丹参注射液对LPS诱导的兔DIC有良好的拮抗作用。

复方丹参与维脑路通注射液在输液中的配伍稳定性

本文观察了维脑路通注射液与复方丹参注射液在０．９％氯化钠注射液中的配伍稳定性，结果表明该两种药物混合配伍无物理、化学变化，在６ｈ内混合静滴是可行的。

复方丹参注射液对心内直视手术心肌缺血再灌注后血清内皮素及前列环素/血栓素A_2比值的影响

目的 :观察复方丹参注射液对心内直视手术心肌缺血再灌注后血清内皮素 (ET) ,前列环素(PGI2 ) ,血栓素A2 (TXA2 )及PGI2 /TXA2 比值的影响。方法 :2 0例先天性室间隔缺损或房间隔缺损患者随机分为治疗组 ( 10例 )及对照组 ( 10例 )。治疗组于手术开始前及复温后心脏复跳前分别静脉注射复方丹参注射液 2 0 0mg/kg ,对照组给予等容量平衡盐溶液。经中心静脉取血测定ET、PGI2 及TXA2 含量 ,并计算PGI2 /TXA2 比值。结果 :在体外循环开始 (T1)后两组血清ET显著降低 (P <0 0 5) ,PGI2 及TXA2 显著增加 (P <0 0 5)。心肌缺血再灌注后对照组ET进行性增加 ,从再灌注后 30min(T3)～ 2 4h(T5)均显著高于治疗组 (P <0 0 5)。再灌注期间治疗组PGI2 及TXA2 降低速度比对照组快 ,但治疗组PGI2 /TXA2 比值高于对照组。T5时治疗组PGI2 及TXA2 含量显著低于对照组 (P <0 0 5) ,但PGI2 /TXA2 比值高于对照组 (P <0 0 5)。血清ET含量与PGI2 /TXA2 比值呈显著负相关 (r =- 0 82 2 ,P <0 0 5)。治疗组患者术后心功能的恢复优于对照组。结论 :复方丹参注射液能升高再灌注期PGI2 /TXA2 比值 ,有效抑制再灌注期内皮素的产生 ,促进缺血再灌注心脏功能的恢复。

丹参对重症急性胰腺炎大鼠诱导型一氧化氮合成酶mRNA的表达与器官损伤的影响

目的探讨重症急性胰腺炎(SAP)大鼠诱导型一氧化氮合成酶(i NOS)mRNA的表达与器官损伤的关系和丹参对其影响。方法Wistar大鼠随机分为3组,模型组和丹参组均以5%牛磺胆酸钠经胰管内逆行注射复制SAP模型,丹参组则在制模后立即肌肉注射丹参注射液(每天2ml/kg),每6h1次,共给药4次;对照组仅行假手术,模型组和对照组同期给予等体积生理盐水。各组大鼠在术后24h测定血淀粉酶(AMY)、一氧化氮(NO)和腹水量,用原位杂交法和图像分析检测胰、肺、肾i NOS mRNA的表达强度,并观察其病理变化。结果血浆中AMY、NO和腹水量,模型组显著高于丹参组及对照组(P<0.05或P<0.01)。i NOS mRNA在胰、肺、肾中的表达模型组、丹参组均增高,模型组显著高于丹参组(P<0.01)。胰、肺、肾病理改变丹参组较模型组减轻。结论SAP时胰、肺、肾组织的i NOS mRNA高表达导致NO过度生成并参与胰腺及胰外器官损伤。丹参早期治疗能抑制胰、肺、肾组织的i NOS mRNA的过度表达,对胰腺、肺及肾起保护作用。

超早期应用复方丹参注射液结合西药基础疗法治疗脑出血的临床观察

在西药基础疗法治疗的同时，以复方丹参注射液超早期应用治疗脑出血３２例，与亚急性期以后应用复方丹参注射液治疗的４０例及单用基础治疗的４７例对照，提示前者在恢复意识和促进脑内血肿吸收方面有显著的作用（Ｐ＜０．０５）。

复方丹参注射液对冠心病患者血管内皮功能及内皮素和一氧化氮合酶基因表达的影响

为探讨复方丹参注射液对冠心病患者血管内皮功能的影响及其作用机制 ,将 84例冠心病患者随机分为对照组、复方丹参注射液治疗组和维生素C治疗组 ,分别于治疗前、后用彩色多普勒检测血管内皮依赖性舒张功能 ,并采血检测内皮素 1和一氧化氮水平及内皮素 1和内皮型一氧化氮合酶mRNA表达。结果发现 ,治疗前 3组间血管内皮依赖性舒张功能、内皮素 1及一氧化氮水平及其mRNA表达比较差异无显著性 ,治疗后复方丹参注射液和维生素C两治疗组血管内皮依赖性舒张功能明显改善 (16 .7%± 5 .0 %和 13.8%± 5 .1%比 9.4 %± 4 .5 %和 9.7%± 4 .1% ,P <0 .0 5 ) ,高于对照组 (11.2 0 %± 3.90 % ,P <0 .0 5 ) ,前者又优于后者 (P <0 .0 5 ) ;复方丹参注射液治疗组内皮素 1水平及其mRNA表达下降 ,内皮型一氧化氮合酶水平及其mRNA表达升高 ,与对照组及治疗前比较差异有显著性 ;维生素C治疗组一氧化氮水平及内皮型一氧化氮合酶mRNA表达也升高 ,但低于复方丹参注射液治疗组 (P <0 .0 5 )。以上提示复方丹参注射液能改善冠心病患者血管内皮功能 ,其机制之一为调节内皮型一氧化氮合酶和内皮素