Identification of Porphyra lines using computerized DNA fingerprinting

RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P. katadai var. Hemiphylla and P. digospermatangia). Eight stable and repeatable RAPD bands amplified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprinting. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting. It can be used in practical Porphyra line identification.

用于紫菜无性系种质鉴定的计算机化DNA指纹的建立

研究用 ＲＡＰＤ技术对条斑紫菜（Ｐｏｒｐｈｙｒａ ｙｅｚｏｅｎｓｉｓ）、坛紫菜（Ｐ． Ｈａｉｔａｎｅｎｓｉｓ）。半叶紫菜（Ｐ．Ｋａｔａｄａｉ ｖａｒ． Ｈｅｍｉｐｈｙｌｌａ）和少精紫菜（Ｐ．Ｏｌｉｇｏｓｐｅｒｍａｔａｎｇｉａ）的１５个无性系丝状体进行了遗传多样分析，用１２０个Ｏｐｅｒｏｎ引物进行了筛选，从中选用来自ＯＰＪ－１８和ＯＰＮ—０２扩增出的８条多态性条带构建了这１５个紫菜无性系的ＤＮＡ指纹图谱，在该图谱中每个紫菜无性系均有各自特异的ＤＮＡ指纹．用１和０分别表示ＤＮＡ带的出现和缺失，将各个无性系的ＤＮＡ指纹用计算机语言表示，建立了１５个紫菜无性系的计算机化ＤＮＡ指纹；在此基础上设计了紫菜无性系种质鉴定的计算机软件ＰｈＧＩ．

罗非鱼微卫星DNA指纹的计算机化鉴定

在奥利亚、尼罗和橙色莫桑比克3种罗非鱼的微卫星DNA指纹数据库构建基础上,为进一步开展实际生产应用而将其鉴定功能计算机化。在数据库中每种鱼都有其特异的DNA指纹,根据等位基因的有或无,把每个个体的指纹转换成由数字1和0组成的指纹数据,变为计算机可识别的语言,在此基础上,利用Net语言开发了一套应用软件——鱼类种质鉴定软件1.0,根据微卫星标记的共显性遗传原理,采用Nei公式,计算查询个体与相关群体的遗传距离,追溯该个体的可能来源。在实际应用中,只需将测试样品的扩增结果转换成指纹数据,由计算机辅助进行种质鉴定。该软件具有普适性,任何物种的微卫星数据都可导入进行相关的鉴定。

运用取指框输入指纹信息的几种方法介绍

在刑侦工作实践中,经常出现待输入的指纹排列位置偏移、指纹方向倾斜、指位错位、左右手颠倒等情况。针对上述情况,文章介绍了几种运用取指框清晰、完整、准确输入指纹的方法。