Advances in Dental Implant Positioning Techniques and Their Clinical Implications

Background: Advancing surgical reconstructive methods and demanding prosthetics need accurate and precise implant placements. Positioning dental implant is vital in both prosthetic and aesthetic perspectives. The optimal three-dimensional placement not only reduces biomechanical complications but also imprecates the odds of implant failure. Materials & Methods: By using robust text mining, searching and retrieval tools, 350 relevant articles were found and then out of them 161 articles were short listed for our review. They included systematic reviews, meta-analyses, case series and experimental studies. Conclusions & Results: Conventional freehand implant placement techniques remain experts’ favorite for uncompromised cases. However, for compromised cases that demand accuracy and predictability, various computer-based methods are in use. While computerized tomography techniques and use of interactive software prevalent in treatment planning, computer-aided design or computer-aided manufacturing (CAD/ CAM) fabricated surgical guides enable implantologists for more successful implantations.

Selection and characterization of the novel anti-human PD-1 FV78 antibody from a targeted epitope mammalian cell-displayed antibody library

Currently,display-based methods are well established and widely used in antibody engineering for affinity maturation and structural stability improvement.We obtained a novel anti-human programmed death 1(PD-1)antibody using computer-aided design and a mammalian cell display technology platform.We used computer-aided modeling and distance geometry methods to predict and assign the key residues that contributed to the binding of human PD-L1 to PD-1.Then,we analyzed the sequence of nivolumab(an anti-human PD-1 antibody,referred to as MIL75 in the article)to determine the template for antibody design and library construction.We identified a series of potential substitutions on the obtained template and constructed a virtual epitope-targeted antibody library based on the physicochemical properties and each possible location of the assigned key residues.The virtual antibody libraries were displayed on the surface of mammalian cells as the antigen-binding fragments of full-length immunoglobulin G.Then,we used flow cytometry and sequencing approaches to sort and screen the candidates.Finally,we obtained a novel anti-human PD-1 antibody named FV78.FV78 competitively recognized the PD-1 epitopes that interacted with MIL75 and possessed an affinity comparable to MIL75.Our results implied that FV78 possessed equivalent bioactivity in vitro and in vivo compared with MIL75,which highlighted the probability and prospect of FV78 becoming a new potential antibody therapy.