Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality c...Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality control of animal drugs. A gas chromatography-mass spectrometric method was built for the quantification analysis of amino acids in the extracts of Comus Caprae Hircus which has been widely used in Chinese medicine compound prescriptions. The full method validation, including the linearity, limit of detection and quantification, repeatability, precision, stability, and recovery test, was examined. The results indicate that the developed method is suitable for the quantification of amino acids in the extracts of Comus Caprae Hircus. The developed method was applied to the quantification analysis of twelve amino acids in different batches of extracts of Comus Caprae Hircus.展开更多
Background: Water dropwort (Oenanthejavanica) as a popular traditional medicine in Asia shows various biological properties including antioxidant activity. In this study, we firstly examined the neuroprotective eff...Background: Water dropwort (Oenanthejavanica) as a popular traditional medicine in Asia shows various biological properties including antioxidant activity. In this study, we firstly examined the neuroprotective effect of Oenanthejavanica extract (OJE) in the hippocampal comus ammonis 1 region (CA 1 region) of the gerbil subjected to transient cerebral ischemia. Methods: Gerbils were established by the occlusion of common carotid arteries for 5 min. The neuroprotective effect of OJE was estimated by cresyl violet staining. In addition, 4 antioxidants (copper, zinc superoxide dismutase [SOD], manganese SOD, catalase, and glutathione peroxidase) immunoreactivities were investigated by immunohistochemistry. Results: Pyramidal neurons in the CA1 region showed neuronal death at 5 days postischemia; at this point in time, all antioxidants immunoreactivities disappeared in CA1 pyramidal neurons and showed 100 mg/kg, OJE protected CA 1 pyramidal neurons from ischemic damage in many nonpyramidal cells. Treatment with 200 mg/kg, not In addition, 200 mg/kg OJE treatment increased or maintained antioxidants immunoreactivities. Especially, among the antioxidants, glutathione peroxidase immunoreactivity was effectively increased in the CA 1 pyramidal neurons of the OJE-treated sham-operated and ischemia-operated groups. Conclusion: Our present results indicate that treatment with OJE can protect neurons from transient ischemic damage and that the neuroprotective effect may be closely associated with increased or maintained intracellular antioxidant enzymes by OJE.展开更多
基金Supported by the National Basic Research Program of China(No.2005CB523402) the Program for New Century Excellent Talents in University of China(No.NCET-06-0515)
文摘Amino acids are an important type of bioactive components in Chinese traditional medicines, especially animal drugs. However, few modern analytical methods targeted at amino acids have been developed for the quality control of animal drugs. A gas chromatography-mass spectrometric method was built for the quantification analysis of amino acids in the extracts of Comus Caprae Hircus which has been widely used in Chinese medicine compound prescriptions. The full method validation, including the linearity, limit of detection and quantification, repeatability, precision, stability, and recovery test, was examined. The results indicate that the developed method is suitable for the quantification of amino acids in the extracts of Comus Caprae Hircus. The developed method was applied to the quantification analysis of twelve amino acids in different batches of extracts of Comus Caprae Hircus.
文摘Background: Water dropwort (Oenanthejavanica) as a popular traditional medicine in Asia shows various biological properties including antioxidant activity. In this study, we firstly examined the neuroprotective effect of Oenanthejavanica extract (OJE) in the hippocampal comus ammonis 1 region (CA 1 region) of the gerbil subjected to transient cerebral ischemia. Methods: Gerbils were established by the occlusion of common carotid arteries for 5 min. The neuroprotective effect of OJE was estimated by cresyl violet staining. In addition, 4 antioxidants (copper, zinc superoxide dismutase [SOD], manganese SOD, catalase, and glutathione peroxidase) immunoreactivities were investigated by immunohistochemistry. Results: Pyramidal neurons in the CA1 region showed neuronal death at 5 days postischemia; at this point in time, all antioxidants immunoreactivities disappeared in CA1 pyramidal neurons and showed 100 mg/kg, OJE protected CA 1 pyramidal neurons from ischemic damage in many nonpyramidal cells. Treatment with 200 mg/kg, not In addition, 200 mg/kg OJE treatment increased or maintained antioxidants immunoreactivities. Especially, among the antioxidants, glutathione peroxidase immunoreactivity was effectively increased in the CA 1 pyramidal neurons of the OJE-treated sham-operated and ischemia-operated groups. Conclusion: Our present results indicate that treatment with OJE can protect neurons from transient ischemic damage and that the neuroprotective effect may be closely associated with increased or maintained intracellular antioxidant enzymes by OJE.