Whey and toned milk was effectively used for the development of tea whitener. Cheese whey was concentrated to 12% solids in a vacuum evaporator, pasteurized at 72℃±2℃ for 15 s, diluted back to 11.5% solids and ...Whey and toned milk was effectively used for the development of tea whitener. Cheese whey was concentrated to 12% solids in a vacuum evaporator, pasteurized at 72℃±2℃ for 15 s, diluted back to 11.5% solids and was cold blended with toned milk of 11.5% solids in three ratios viz. 25:75, 50:50 and 75:25 and selected blends were homogenized at 1200-1500 psi. Tea was prepared by adding 80 mL whitener to 100 mL decoction and addition of sugar at 8% (w/v). The tea whitener was selected based on the sensory attributes of tea beverages. The colour of the beverages, tea whiteners and decoction were analyzed by reflectance meter and computer vision image analysis technique. The viscosity of the beverage was tested using a Brookfield viscometer. Coffee stability test was done to assess the stability of tea whitener under acidic conditions. Standard plate count, Coliform count and Presumptive Coliform test were done to assess the microbiological quality of the whitener. Homogenization had a positive impact on the viscosity and colour. The reflectance value of tea prepared from 25% homogenised whey milk blend and toned milk were in the range of 43-46 and 45-49, respectively. The viscosity of tea was found to increase with homogenization and whey levels. The sedimentation values of 25% whey blends and toned milk were 1.25 mL and 1 mL respectively. The shelf life of the blends was 2 days under refrigerated conditions. It can be concluded that a satisfactory tea whitener can be prepared by blending whey with toned milk in 25:75 proportion followed by homogenization.展开更多
The objective of the experiment was to determine the effect of feeding three levels (T3.5, T7.0 and T10.5) of energy concentrate (3.5, 7.0 and 10.5 kg cow-1 day-1) on total dry matter (DM) and energy intakes, milk yie...The objective of the experiment was to determine the effect of feeding three levels (T3.5, T7.0 and T10.5) of energy concentrate (3.5, 7.0 and 10.5 kg cow-1 day-1) on total dry matter (DM) and energy intakes, milk yield and composition, nutritional value of milk and rumen pasture neutral detergent fiber (NDF) digestion in grazing dairy cows. Twenty-one multiparous Holstein cows in early lactation (70.2 ± 13 days postpartum) producing 37.1 (±4.7) kg of milk were assigned to a 3 treatments (7 cows/treatment) × 3 periods Latin square design. Parameters of ruminal environment and pasture NDF degradation were obtained using 3 additional cows of the same breed fitted with rumen cannulae. On a wet basis, concentrate was composed by corn grain (68%), soybean meal (22%), wheat bran (8%) and a vitamin-mineral nucleus with monensin. Pasture (Medicago sativa, sp) was used in a rotational grazing system with an herbage allowance of 30 kg DM cow-1 d-1. Yield (kg cow-1 d-1) of fat corrected milk (4% FCM) resulted higher (p < 0.05) in T7.0 (29.0) compared to T3.5 (26.8) but similar to T10.5 (30.2). Milk and protein yields were linearly increased (p < 0.01) by concentrate intake. Milk fat (3.13 g/100g) and total solid contents (11.79 g/100g) did not differ whereas milk protein (p < 0.03) and casein (p < 0.01) levels (g/100g) increased linearly from 3.05 to 3.10 (protein) and from 2.42 to 2.47 (casein). Pasture intake decreased but total DM and energy consumption were enhanced showing addition effects after concentrate feeding. Body weight (BW) loss and plasmatic levels of non esterified fatty acids (NEFA) decreased with concentrate intake. Circulating levels of insulin-like growth factor-I (IGF-I) were higher (p < 0.05) in T10.5 while those of glucose, plasma urea nitrogen, insulin and somatotrophin were not affected. Ruminal pH and acetate concentration resulted lower (p < 0.05) in T10.5 when compared to T3.5. The acetate:propionate ratio decreased (p < 0.01) from 4.25 in T3.5 to 3.08 in T10.5 and ruminal ammonia nitrogen levels tended (p < 0.07) to be lower as concentrate intake increased. Kinetics parameters of NDF degradation remained unchanged. The potential hypercholesterolemic fatty acids (FA) of milk (C12:0 to C16:0.) remained unchanged as concentrate intake increased. Milk content of linolenic acid decreased and the n-6:n-3 ratio increased with concentrate intake from 1.56 (T3.5) to 2.57 (T10.5) remaining below the recommended values for human health (<4:1). Milk content of antioxidant vitamins was not significantly altered even when pasture DM intake fall in T10.5 compared to T3.5. Increased consumption of a starch-rich concentrate up to 40% of DM intake of cows showed additive effects on total DM and energy intakes improving milk yield, milk protein and casein contents without negative effects on milk fat concentration or yield. Pasture fiber digestion and nutritional parameters linked to healthy value of milk fat were not affected.展开更多
Background: It is well known that feeding a high concentrate(HC) diet to lactating ruminants likely induces subacute ruminal acidosis(SARA) and leads to a decrease in milk fat production. However, the effects of ...Background: It is well known that feeding a high concentrate(HC) diet to lactating ruminants likely induces subacute ruminal acidosis(SARA) and leads to a decrease in milk fat production. However, the effects of feeding a HC diet for long periods on milk fatty acids composition and the mechanism behind the decline of milk fat still remains poorly understood. The aim of this study was to investigate the impact of feeding a HC diet to lactating dairy goats on milk fat yield and fatty acids composition with an emphasis on the mechanisms underlying the milk fat depression. Seventeen mid-lactating dairy goats were randomly allocated to three groups. The control treatment was fed a low-concentrate diet(35% concentrate, n = 5, LC) and there were two high-concentrate treatments(65% concentrate, HC), one fed a high concentrate diet for a long period(19 wks, n = 7, HL); one fed a high concentrate diet for a short period of time(4 wk, n = 5, HS). Milk fat production and fatty acids profiles were measured. In order to investigate the mechanisms underlying the changes in milk fat production and composition,the gene expression involved in lipid metabolism and DNA methylation in the mammary gland were also analyzed.Results: Milk production was increased by feeding the HC diet in the HS and HL groups compared with the LC diet(P 〈 0.01), while the percentage of milk fat was lower in the HL(P 〈 0.05) but not in the HS group. The total amount of saturated fatty acids(SFA) in the milk was not changed by feeding the HC diet, whereas the levels of unsaturated fatty acids(UFA) and monounsaturated fatty acids(MUFA) were markedly decreased in the HL group compared with the LC group(P 〈 0.05). Among these fatty acids, the concentrations of C15:0(P 〈 0.01), C17:0(P 〈 0.01), C17:1(P 〈 0.01), C18:1 n-9 c(P 〈 0.05), C18:3 n-3 r(P 〈 0.01) and C20:0(P 〈 0.01) were markedly lower in the HL group, and the concentrations of C20:0(P 〈 0.05) and C18:3 n-3 r(P 〈 0.01) were lower in the HS group compared with the LC group. However, the concentrations of C18:2 n-6 c(P 〈 0.05) and C20:4 n-6(P 〈 0.05) in the milk fat were higher in the HS group. Real-time PCR results showed that the m RNA expression of the genes involved in milk fat production in the mammary gland was generally decreased in the HL and HS groups compared with the LC group. Among these genes, ACSL1, ACSS1 & 2, ACACA, FAS, SCD, FADS2, and SREBP1 were downregulated in the mammary gland of the HL group(P 〈 0.05), and the expressions of ACSS2, ACACA, and FADS2 m RNA were markedly decreased in the HS goats compared with the LC group(P 〈 0.05). In contrast to the gene expression, the level of DNA methylation in the promoter regions of the ACACA and SCD genes was increased in the HL group compared with the LC group(P 〈 0.05). The levels of ACSL1 protein expression and FAS enzyme activity were also decreased in the mammary gland of the HL compared with the LC group(P 〈 0.05).Conclusions: Long-term feeding of a HC diet to lactating goats induced milk fat depression and FAs profile shift with lower MUFAs but higher SFAs. A general down-regulation of the gene expression involved in the milk fat production and a higher DNA methylation in the mammary gland may contribute to the decrease in milk fat production in goats fed a HC diet for long time periods.展开更多
Objective To determine the concentration changes of medroxyprogesterone acetate (MPA) in serum and milk in the Chinese lactating women who used Depo Geston Methods Ten postpartum and breastfeeding women received an ...Objective To determine the concentration changes of medroxyprogesterone acetate (MPA) in serum and milk in the Chinese lactating women who used Depo Geston Methods Ten postpartum and breastfeeding women received an injectable contraceptive of Depo Geston (depo medroxyprogesterone acetate, DMPA, 150 mg/ampoule). Serum and milk samples were collected in the 1^st, 2^st, 4^th, 6^th, 8^th 10^th and 12^th week after contraceptive injection. The concentrations of medroxyprogesterone acetate (MPA) in serum and milk were determined by radioimmunoassay. Results The highest MPA concentration in serum was observed at the 1^st week and then the level of serum MPA decreased obviously at the 2^na and 4^th week. After the 4^th week, the decline of serum MPA level slowed down. The concentration of milk MPA reached the highest level at 1^th week. But at the 2^nd week the average level of MPA in milk dropped by half The MPA concentration in the milk was changed in the range of 5.2-8.3 ng/mL in the following 10 weeks. During observation, the average ratios of concentration and area under curve (AUC) in milk/serum were both 0.55. There was a wide variation in MPA concentrations and ratios of milk/serum between subjects. Conclusion MPA concentrations maintained a certaim level at the 12^th week after DMPA injection. However, whether mather's intake of DMPA during feeding period has effect on children's growth, espcially pubertal growth, deserves further studies.展开更多
Human milk is the ideal nutritional support for premature neonates. Considering the need for aggregating nutritional value to human milk provided to such vulnerable group of infants, human milk was concentrated by the...Human milk is the ideal nutritional support for premature neonates. Considering the need for aggregating nutritional value to human milk provided to such vulnerable group of infants, human milk was concentrated by the block freeze concentration technique. The effects of freeze concentration on the physicochemical properties, the efficiency of the process, color parameters, and the density and dynamic viscosity of human milk were assessed. The freeze concentration technology was used to successfully concentrate human milk to a factor equal to 180.48% and 72% of total solid retention in the second stage of freeze concentration. The values observed in the concentrates for the biochemical properties showed that the fraction of concentrated fluid human milk of the second stage (C2) presented elevated amounts of carbohydrates, protein and energy. The elevated caloric value observed in the ice fraction of the first stage (I1) refers to the retention of lipids in it. When added to human milk, C2 and I1 may satisfy the special requisites of nutrients and energy to guarantee the growth and development of preterm neonates.展开更多
传统牛乳浓缩工艺通过加热蒸发水分实现浓缩,具有蛋白质受热变性、感官品质降低、生产效率低及无法去除乳糖等问题。引入超滤技术以脱脂牛乳为原料制备无乳糖牛乳蛋白浓缩物(milk protein concentrate,MPC)以弥补传统工艺的缺陷,并对分...传统牛乳浓缩工艺通过加热蒸发水分实现浓缩,具有蛋白质受热变性、感官品质降低、生产效率低及无法去除乳糖等问题。引入超滤技术以脱脂牛乳为原料制备无乳糖牛乳蛋白浓缩物(milk protein concentrate,MPC)以弥补传统工艺的缺陷,并对分离过程中的分离系统、洗滤模式及操作条件等工艺进行优化。最终采用分离精度为10 kDa的聚醚砜中空纤维超滤膜组件在自制膜分离系统中以40℃、0.1 MPa的操作条件下2倍浓缩、3次反洗补水洗滤制备了乳糖质量浓度为1.17 g/L、蛋白收率为94.0%、蛋白质量浓度为40.01 g/L、pH值为6.98、乳糖脱除率为96.5%的无乳糖MPC。超滤浓缩技术有效去除了乳糖,且工艺优化显著提高了生产效率与产品质量,制备出的无乳糖MPC可实现乳产品组分的优化。展开更多
常温再制干酪制品是指经超高温瞬时灭菌(Ultra-high temperature treated, UHT)处理后可常温贮藏的灭菌型干酪制品,是我国乳品工业的新热点。然而,干酪在UHT处理后易失稳发生凝胶结构重构,在贮藏期间出现析水等质构问题,影响产品品质。...常温再制干酪制品是指经超高温瞬时灭菌(Ultra-high temperature treated, UHT)处理后可常温贮藏的灭菌型干酪制品,是我国乳品工业的新热点。然而,干酪在UHT处理后易失稳发生凝胶结构重构,在贮藏期间出现析水等质构问题,影响产品品质。为阐明蛋白原料对常温再制干酪制品析水性的影响及机制,筛选了3种不同的蛋白原料:膜过滤酪蛋白胶束、浓缩牛奶蛋白、凝乳酶酪蛋白,分析其蛋白质含量、组成以及粒径、电位、水合性和钙离子分布等理化特性,进而分析了蛋白原料对常温再制干酪制品析水性和质构特性的影响规律,并从水分分布和微观结构层面解析蛋白原料对常温再制干酪制品析水性的影响机制。结果表明:3种蛋白原料的蛋白质含量和组成存在显著差异,浓缩牛奶蛋白的乳清蛋白占总蛋白质量分数比膜过滤酪蛋白胶束高7.81个百分点,而凝乳酶酪蛋白中不含乳清蛋白;在水合性方面,浓缩牛奶蛋白和膜过滤酪蛋白胶束相近,均显著高于凝乳酶酪蛋白。蛋白原料显著影响常温再制干酪制品析水性,贮藏90 d时,由凝乳酶酪蛋白制备的常温再制干酪制品析水率最高,而由浓缩牛奶蛋白制备的常温再制干酪制品的析水率最低,表面析水率和离心析水率分别为0.42%和1.10%。水分分布和微观结构结果显示,蛋白原料通过影响干酪制品中水分的存在状态和三维网络空间结构,从而影响常温再制干酪制品的析水性。研究解析了不同蛋白原料对常温再制干酪制品析水性的影响及机制,为常温再制干酪制品析水性的改善提供了理论依据。展开更多
文摘Whey and toned milk was effectively used for the development of tea whitener. Cheese whey was concentrated to 12% solids in a vacuum evaporator, pasteurized at 72℃±2℃ for 15 s, diluted back to 11.5% solids and was cold blended with toned milk of 11.5% solids in three ratios viz. 25:75, 50:50 and 75:25 and selected blends were homogenized at 1200-1500 psi. Tea was prepared by adding 80 mL whitener to 100 mL decoction and addition of sugar at 8% (w/v). The tea whitener was selected based on the sensory attributes of tea beverages. The colour of the beverages, tea whiteners and decoction were analyzed by reflectance meter and computer vision image analysis technique. The viscosity of the beverage was tested using a Brookfield viscometer. Coffee stability test was done to assess the stability of tea whitener under acidic conditions. Standard plate count, Coliform count and Presumptive Coliform test were done to assess the microbiological quality of the whitener. Homogenization had a positive impact on the viscosity and colour. The reflectance value of tea prepared from 25% homogenised whey milk blend and toned milk were in the range of 43-46 and 45-49, respectively. The viscosity of tea was found to increase with homogenization and whey levels. The sedimentation values of 25% whey blends and toned milk were 1.25 mL and 1 mL respectively. The shelf life of the blends was 2 days under refrigerated conditions. It can be concluded that a satisfactory tea whitener can be prepared by blending whey with toned milk in 25:75 proportion followed by homogenization.
文摘The objective of the experiment was to determine the effect of feeding three levels (T3.5, T7.0 and T10.5) of energy concentrate (3.5, 7.0 and 10.5 kg cow-1 day-1) on total dry matter (DM) and energy intakes, milk yield and composition, nutritional value of milk and rumen pasture neutral detergent fiber (NDF) digestion in grazing dairy cows. Twenty-one multiparous Holstein cows in early lactation (70.2 ± 13 days postpartum) producing 37.1 (±4.7) kg of milk were assigned to a 3 treatments (7 cows/treatment) × 3 periods Latin square design. Parameters of ruminal environment and pasture NDF degradation were obtained using 3 additional cows of the same breed fitted with rumen cannulae. On a wet basis, concentrate was composed by corn grain (68%), soybean meal (22%), wheat bran (8%) and a vitamin-mineral nucleus with monensin. Pasture (Medicago sativa, sp) was used in a rotational grazing system with an herbage allowance of 30 kg DM cow-1 d-1. Yield (kg cow-1 d-1) of fat corrected milk (4% FCM) resulted higher (p < 0.05) in T7.0 (29.0) compared to T3.5 (26.8) but similar to T10.5 (30.2). Milk and protein yields were linearly increased (p < 0.01) by concentrate intake. Milk fat (3.13 g/100g) and total solid contents (11.79 g/100g) did not differ whereas milk protein (p < 0.03) and casein (p < 0.01) levels (g/100g) increased linearly from 3.05 to 3.10 (protein) and from 2.42 to 2.47 (casein). Pasture intake decreased but total DM and energy consumption were enhanced showing addition effects after concentrate feeding. Body weight (BW) loss and plasmatic levels of non esterified fatty acids (NEFA) decreased with concentrate intake. Circulating levels of insulin-like growth factor-I (IGF-I) were higher (p < 0.05) in T10.5 while those of glucose, plasma urea nitrogen, insulin and somatotrophin were not affected. Ruminal pH and acetate concentration resulted lower (p < 0.05) in T10.5 when compared to T3.5. The acetate:propionate ratio decreased (p < 0.01) from 4.25 in T3.5 to 3.08 in T10.5 and ruminal ammonia nitrogen levels tended (p < 0.07) to be lower as concentrate intake increased. Kinetics parameters of NDF degradation remained unchanged. The potential hypercholesterolemic fatty acids (FA) of milk (C12:0 to C16:0.) remained unchanged as concentrate intake increased. Milk content of linolenic acid decreased and the n-6:n-3 ratio increased with concentrate intake from 1.56 (T3.5) to 2.57 (T10.5) remaining below the recommended values for human health (<4:1). Milk content of antioxidant vitamins was not significantly altered even when pasture DM intake fall in T10.5 compared to T3.5. Increased consumption of a starch-rich concentrate up to 40% of DM intake of cows showed additive effects on total DM and energy intakes improving milk yield, milk protein and casein contents without negative effects on milk fat concentration or yield. Pasture fiber digestion and nutritional parameters linked to healthy value of milk fat were not affected.
基金supported by the National Nature Science Foundation of China(project no.31572433)the National Key R&D Program(2016YFD0501203)+1 种基金the Program for New Century Excellent Talents in University(NCET-13-0862)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Background: It is well known that feeding a high concentrate(HC) diet to lactating ruminants likely induces subacute ruminal acidosis(SARA) and leads to a decrease in milk fat production. However, the effects of feeding a HC diet for long periods on milk fatty acids composition and the mechanism behind the decline of milk fat still remains poorly understood. The aim of this study was to investigate the impact of feeding a HC diet to lactating dairy goats on milk fat yield and fatty acids composition with an emphasis on the mechanisms underlying the milk fat depression. Seventeen mid-lactating dairy goats were randomly allocated to three groups. The control treatment was fed a low-concentrate diet(35% concentrate, n = 5, LC) and there were two high-concentrate treatments(65% concentrate, HC), one fed a high concentrate diet for a long period(19 wks, n = 7, HL); one fed a high concentrate diet for a short period of time(4 wk, n = 5, HS). Milk fat production and fatty acids profiles were measured. In order to investigate the mechanisms underlying the changes in milk fat production and composition,the gene expression involved in lipid metabolism and DNA methylation in the mammary gland were also analyzed.Results: Milk production was increased by feeding the HC diet in the HS and HL groups compared with the LC diet(P 〈 0.01), while the percentage of milk fat was lower in the HL(P 〈 0.05) but not in the HS group. The total amount of saturated fatty acids(SFA) in the milk was not changed by feeding the HC diet, whereas the levels of unsaturated fatty acids(UFA) and monounsaturated fatty acids(MUFA) were markedly decreased in the HL group compared with the LC group(P 〈 0.05). Among these fatty acids, the concentrations of C15:0(P 〈 0.01), C17:0(P 〈 0.01), C17:1(P 〈 0.01), C18:1 n-9 c(P 〈 0.05), C18:3 n-3 r(P 〈 0.01) and C20:0(P 〈 0.01) were markedly lower in the HL group, and the concentrations of C20:0(P 〈 0.05) and C18:3 n-3 r(P 〈 0.01) were lower in the HS group compared with the LC group. However, the concentrations of C18:2 n-6 c(P 〈 0.05) and C20:4 n-6(P 〈 0.05) in the milk fat were higher in the HS group. Real-time PCR results showed that the m RNA expression of the genes involved in milk fat production in the mammary gland was generally decreased in the HL and HS groups compared with the LC group. Among these genes, ACSL1, ACSS1 & 2, ACACA, FAS, SCD, FADS2, and SREBP1 were downregulated in the mammary gland of the HL group(P 〈 0.05), and the expressions of ACSS2, ACACA, and FADS2 m RNA were markedly decreased in the HS goats compared with the LC group(P 〈 0.05). In contrast to the gene expression, the level of DNA methylation in the promoter regions of the ACACA and SCD genes was increased in the HL group compared with the LC group(P 〈 0.05). The levels of ACSL1 protein expression and FAS enzyme activity were also decreased in the mammary gland of the HL compared with the LC group(P 〈 0.05).Conclusions: Long-term feeding of a HC diet to lactating goats induced milk fat depression and FAs profile shift with lower MUFAs but higher SFAs. A general down-regulation of the gene expression involved in the milk fat production and a higher DNA methylation in the mammary gland may contribute to the decrease in milk fat production in goats fed a HC diet for long time periods.
文摘Objective To determine the concentration changes of medroxyprogesterone acetate (MPA) in serum and milk in the Chinese lactating women who used Depo Geston Methods Ten postpartum and breastfeeding women received an injectable contraceptive of Depo Geston (depo medroxyprogesterone acetate, DMPA, 150 mg/ampoule). Serum and milk samples were collected in the 1^st, 2^st, 4^th, 6^th, 8^th 10^th and 12^th week after contraceptive injection. The concentrations of medroxyprogesterone acetate (MPA) in serum and milk were determined by radioimmunoassay. Results The highest MPA concentration in serum was observed at the 1^st week and then the level of serum MPA decreased obviously at the 2^na and 4^th week. After the 4^th week, the decline of serum MPA level slowed down. The concentration of milk MPA reached the highest level at 1^th week. But at the 2^nd week the average level of MPA in milk dropped by half The MPA concentration in the milk was changed in the range of 5.2-8.3 ng/mL in the following 10 weeks. During observation, the average ratios of concentration and area under curve (AUC) in milk/serum were both 0.55. There was a wide variation in MPA concentrations and ratios of milk/serum between subjects. Conclusion MPA concentrations maintained a certaim level at the 12^th week after DMPA injection. However, whether mather's intake of DMPA during feeding period has effect on children's growth, espcially pubertal growth, deserves further studies.
文摘Human milk is the ideal nutritional support for premature neonates. Considering the need for aggregating nutritional value to human milk provided to such vulnerable group of infants, human milk was concentrated by the block freeze concentration technique. The effects of freeze concentration on the physicochemical properties, the efficiency of the process, color parameters, and the density and dynamic viscosity of human milk were assessed. The freeze concentration technology was used to successfully concentrate human milk to a factor equal to 180.48% and 72% of total solid retention in the second stage of freeze concentration. The values observed in the concentrates for the biochemical properties showed that the fraction of concentrated fluid human milk of the second stage (C2) presented elevated amounts of carbohydrates, protein and energy. The elevated caloric value observed in the ice fraction of the first stage (I1) refers to the retention of lipids in it. When added to human milk, C2 and I1 may satisfy the special requisites of nutrients and energy to guarantee the growth and development of preterm neonates.
文摘常温再制干酪制品是指经超高温瞬时灭菌(Ultra-high temperature treated, UHT)处理后可常温贮藏的灭菌型干酪制品,是我国乳品工业的新热点。然而,干酪在UHT处理后易失稳发生凝胶结构重构,在贮藏期间出现析水等质构问题,影响产品品质。为阐明蛋白原料对常温再制干酪制品析水性的影响及机制,筛选了3种不同的蛋白原料:膜过滤酪蛋白胶束、浓缩牛奶蛋白、凝乳酶酪蛋白,分析其蛋白质含量、组成以及粒径、电位、水合性和钙离子分布等理化特性,进而分析了蛋白原料对常温再制干酪制品析水性和质构特性的影响规律,并从水分分布和微观结构层面解析蛋白原料对常温再制干酪制品析水性的影响机制。结果表明:3种蛋白原料的蛋白质含量和组成存在显著差异,浓缩牛奶蛋白的乳清蛋白占总蛋白质量分数比膜过滤酪蛋白胶束高7.81个百分点,而凝乳酶酪蛋白中不含乳清蛋白;在水合性方面,浓缩牛奶蛋白和膜过滤酪蛋白胶束相近,均显著高于凝乳酶酪蛋白。蛋白原料显著影响常温再制干酪制品析水性,贮藏90 d时,由凝乳酶酪蛋白制备的常温再制干酪制品析水率最高,而由浓缩牛奶蛋白制备的常温再制干酪制品的析水率最低,表面析水率和离心析水率分别为0.42%和1.10%。水分分布和微观结构结果显示,蛋白原料通过影响干酪制品中水分的存在状态和三维网络空间结构,从而影响常温再制干酪制品的析水性。研究解析了不同蛋白原料对常温再制干酪制品析水性的影响及机制,为常温再制干酪制品析水性的改善提供了理论依据。