A comparative study on the angiogenic activity of condyloma acuminatum and basal cell carcinoma

Recently, the study on the extraction, component analysis and function of angiogenic factor (AF) has become an important subject in anti-tumor research and made a great progress. Therefore, the imitation of the tumor's angiogenic activity (AGA) possibly helps to find a new method to treat ischemic diseases by blood vessel proliferation. Most of the parenchymatous malignant tumors have AGA. We studied the early stage condyloma acuminatum (CA) . After transplanting CA onto the chorioallantoic membrane (CAM) of chick embryos, we observed the AGA of CA and carefully compared it with that of basal cell carcinoma which is generally considered to have strong AGA. Meanwhile, we explored the way by which the AF of CA comes into being. The results demonstrated that CA, a benign neoplastic lesion, at its early stage, also has AGA. It is postulated that the responsible material is a certain form of AF. Therefore, the study on AGA of benign lesions (such as CA) and its physical basis will have potential clinical significance.

The Condyloma Acuminatum’s Recurrence Can Be Reduced by Lesional Auto-Transplantation

Background: Condyloma acuminatum (CA), also known as a genital wart (GW), is a sexually transmitted disease caused by human papillomavirus (HPV). HPV lesions, recurrence tendency, and risk of malignant formation are primarily dependent on the person’s immunity level. GW recurrence is a major challenge in CA treatment. The aim of this study was to explore how lesional auto-transplantation (LT) can be used to treat CA and decrease its recurrence. Methods: We treated CA through the preparation and implantation of tissue from nine CA patients in our dermatology clinic. Transplantation of small pieces of HPV lesions to the subcutaneous fat of the inguinal region was carried out with the help of a simple surgical method under local anesthesia. Patients were followed up for six to eight months. We searched PubMed and the Web of Science for treatment options for CA to compare our treatment method recurrence rate with existing treatment options. Results: During three months of follow-up, seven patients experienced no recurrence of condyloma lesions, while two patients experienced recurrence of small lesions of condyloma acuminatum. The recurrence rate of CA once treated by auto-transplantation was relatively low compared to other CA treatment options. Conclusions: Transplantation of HPV lesions to the inguinal area reduces the recurrence rate in sexually active individuals. The 2/9 recurrence after implantation could be due to poor hygiene, sexual relationships or immune factors.

Fas and Bc1-2 EXpression on T Lymphocyte Subsets in the Peripheral Blood of Relapsing Patients with Condyloma Acuminatum

Objective: To study the expression of Fas and Bcl-2proteins on T lymphocyte subsets in the peripheralblood of relapsing patients with condyloma acuminatum(CA) and healthy controls. Methods: Flow cytometry (permeabization andstaining procedure with conjugated antibodies) wasused. Results: We observed that the expression of Fasprotein on CD4^+ T lymphocyte subset of CA patientswas significantly higher than that of healthy controls(P<0.01). Conclusions: Increased expression of Fas proteinon CD4^+ T lymphocyte subset may be a cause of de-creased percentage of CD4^+ T lymphocyte subset. Thisinduces the increased ratio of CD4^+/CD8^+.

Observation of Ultrastructure in Lesions of Condyloma Acuminatum

Objective: To explore the effect of morphological changes in the development of condyloma acuminatum (CA) Materials and Methods: Lesions in five patients with CA were observed . Results: Upon electron microscopy, the most characteristic feature of the lesions important for diagnosis, was the presence of distinct perinuclear vacuolizations, or so-called koilocytes, among some epithelial cells. These cells possessed hyperchromatic nuclei, swollen mitochondria, dilated endoplasmic reticulum and dissolved glycogen. There were interchromatin granules and perichromatin granules in some nuclei. Moreover some virus particles were also seen in the nuclei of some infected cells. Conclusions: The ultrastructural findings may be used to histopathologically explain the pathogenesis and mechanism of this disease, and it is helpful for diagnosis of CA .

Effect of Activation-induced Cell Death of Peripheral Blood Mononuclear cells in Patients with Condyloma Acuminatum

Objective: To investigate the effect of activation-induced cell death (AICD) on cellular immune function in the condyloma acuminatum(CA). Methods: Peripheral blood mononuclear cells (PBMC) were isolated from normal healthy individuals (control group) and patients with CA. PBMC were cultured with PHA-P for 48h in vitro. Apoptosis of the PBMC was detected by flow cytometry. Supernatant cytokines (IL-2 and IL-10) were assayed by ELISA. Results: The rate of PBMC apoptosis in both CA group and control group in fresh PBMC was very low and similar in both groups(P>0.05). The rate of PBMC apoptosis within the CA group was noticeably increased compared to that of the control (P<0.001)af-ter PBMC were cultured for 48h. The level of IL-2 was significantly lower in the CA group than in the control group (P<0.001), The level of IL-10 was significantly higher in the CA group compared to thecontrol group(P<0.001). Conclusion: Study results indicate that AICD may affect cellular mediated immune function and play an important role in the pathogenesis of CA.

The Lymphoblastoid Cell Lines of Recurrence Condyloma Acuminatum Patients Produce Lower Level of Tumor Necrosis Factor Stimulated with LPS

To study the mechanism of Condyloma acuminatum (CA) recurrence, and the association of CA recurrence with the ability of the host derived lymphoblastoid cell lines (LCL) stimulated by LPS to produce tumor necrosis factor (TNF), EBV-transformed B LCL were used as TNF producing cells The ability of LCL stimulated by LPS to produce TNF was measured by bioassay The results showed that the LCL from CA patients (including recurrent and non-recurrent CA patients) produced similar level of TNF stimulated by LPS to that of normal controls (29 54%±11 28% vs 34 31%±11 46%, P =0 1498) The LCL of CA recurrent patients produced significantly lower amount of TNF than that of non-recurrent CA patients (23 72%±7 41% vs 37 33%±11 10%, P =0 0032) Compared with the normal controls, CA recurrent patients showed a decreased ability to produce TNF (23 72%±7 41 vs 34 31%±11 46, P =0 0054), whereas CA non recurrent patients had the similar ability to the controls (37 33%±11 10 vs 34 31%±11 46, P =0 4914) It was concluded that the onset of CA was not relevant to the individual's ability to produce TNF But the recurrence of CA was associated with the ability to produce TNF It was also indicated that the TNF involved cellular immunity might play an important role in the clearance of the residual HPV by the host after treatment

Telomerase Activity in Condyloma Acuminatum Tissue with Different HPV Types

Summary: The telomerse activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with gennital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7 % in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.

Expression of CD40 and CD40L on Peripheral Blood Mononuclear Cells in Patients with Condyloma Acuminatum

To observe the expression of CD40/CD40L on peripheral blood mononuclear cells （PBMC） in patients with eondyloma aeuminatum （CA）, flow eytometry was employed to examine the expression of CD40 and CD40L on PMBC in 36 patients with CA and 20 healthy controls. Our results showed that mean level of CD40 expression in CA patients was significantly lower than that in the controls （6.58 %±2.74 % vs 14.81 %±6.12 %, t=5. 703, P〈0.05）; the average level of CD40L in CA patients was also significantly lower than that in the controls （0.73 % ±0.54 % vs 2.67 %±2.43 %, t=3. 532, P〈0.05）. Our resutls suggest that the reduced costimulatory interaction of CD40 and CD40L in CA patients may be one of the important factors responsible for the low cellular immunity.

Changes of Circulating Platelet-Derived Growth Factor and Granulocyte Colony-Stimulating Factor in Patients with Condyloma Acuminatum

Objective: To study the expression levels of platelet-derived growth factor (PDGF) and granulocyte colony-stimulatingfactor (G-CSF) in peripheral blood and their role in thepathogenesis of Condyloma acuminatum (CA). Methods: Sera were taken from 70 patients with Condylomaacuminatum and compared with 35 healthy controls. PDGFand G-CSF in serum were quantitated using a dual antibodysandwich enzyme-linked immunoabsorbent assay (ELISA). Results: Serum concentrations of PDGF and G-CSF weresignificantly increased in patients with Condylomaacuminatum (CA) compared to controls (P<0.001 and P<0.005respectively). Serum levels of PDGF and G-CSF correlatedwith clinical severity of CA, but no significant difference wasobserved between different duration of disease groups. Asignificant positive correlation was noticed between neutrophilcount and G-CSF levels (γ=0.38, P<0.001), and the neutrophilcount showed no significant correlation with PDGE Conclusion: The results indicated that increased expressionof PDGF an -CSF in peripheral blood might be involved in pathogenesis of CA.

Perianal Giant Condyloma Acuminatum:A Case Report

Rare giant condyloma acuminatum （CA） reported by this paper is an interesting unusual case in China. Giant CA is a tumor that primarily affects the genital and perianal areas. Its feature is the high rate of local recurrence and transformation into squamous cell carcinoma. Making choice of wide surgical excision and using interferon as antiviral and immunoenhancement for CA after operation, we obtain satisfactory functional and cosmetic results.

CO_(2)LASER PLUS PHOTODYNAMIC THERAPY VERSUS CO_(2)LASER IN THE TREATMENT OF CONDYLOMA ACUMINATUM:A RANDOMIZED COMPARATIVE STUDY

To compare the efficacy and safety of CO_(2)laser plus 5-aminolevulinic acid(ALA)photodynamic therapy(PDT)with CO_(2)laser for the treatment of multiple condyloma acuminatum(CA),120 patients with multiple CA were allocated into two groups—combined group(CO_(2)laser plus ALA-PDT,n=60)and CO_(2)laser group(CO_(2)laser plus placebo-PDT,n=60).After CO_(2)laser,a 20%ALA or a placebo solution was applied to the CA area 3 h before illumination with red light(635 nm,100mW/cm^(2),80 J/cm^(2)).The treatment was repeated seven days after the first treatment if the lesions were not completely resolved.The complete response rate,recurrence rate and adverse effects in the two groups were analyzed.After two treatments,the complete response rates in the CO_(2)laser group and combined group were 100%(509/509)and 100%(507/507)in the CA(p>0:05),respectively.The recurrence rates in the CO_(2)laser group and combined group were 44.9%(229/509)and 10.6%(54/507)in the CA(p<0:05),respectively.The adverse effects in CO_(2)laser group was more than that in combined group.The combined group is a more effective treatment for multiple CA compared with CO_(2)laser group.T/S.Style the highlighted text as abstract.

Unusual presentation of penile giant condyloma acuminatum with spontaneous prepuce perforation: A case report

BACKGROUND Giant condyloma acuminatum(GCA)is an uncommon condition affecting the anogenital area.CASE SUMMARY Here,we report an 88-year-old male patient who presented with a 5-year history of a progressive appearance of multiple cauliflower-like warts over his penile shaft,pubis region,and groin as well as urine leakage along the distal penile shaft.Physical examination revealed an ulcerative skin perforation with pus discharge under the distal prepuce base,which was initially suspected to be a urethral fistula.However,during surgery,it was discovered that the perforation was caused by a giant condyloma lesion that had obliterated the prepuce opening,with infection and high pressure causing subsequent skin perforation.He underwent circumcision and wide excision with electrocauterization of the warts.He was discharged after the surgery,and the residual lesion was treated with imiquimod and low-dose oral tegafur-uracil.CONCLUSION Penile GCA can cause prepuce perforation and can be postoperatively treated with imiquimod and low-dose oral tegafur-uracil.

A Correlative Analysis of Cervical Lesions in Patients with Vulva Condyloma Acuminatum

OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus （HPV） infections and cervical epithelium lesions. METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, highrisk HPV-DNA test and cervical bioscopy. RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV （＋） infections were detected in 68.7% （287/418） of the cases. Among those patients, 20,6% （59/287） had concurrent subclinical cervical intraepithelial neoplastic （CIN） lesions. Among the high-risk HPV（-） 31.3 % （131）, patients 7,6% （10/131） had concurrent subclinical CIN lesions, Pathological examination results： cervicitis, 167 （40.0%）; cervical HPV infection, 182 （43.5%）; CIN-Ⅰ, 51（12.2%）; CIN- Ⅱ, 16 （3.83%）; CIN-Ⅲ, 2 （0,5%）; cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia （VIN） Ⅱ-Ⅲ, CONCLUSION It was concluded that simultaneous cervical HPV infection of many types,was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.

MICROSTRIP APPLICATOR FOR TREATMENT OF CERVICAL EROSION AND CERVICAL CONDYLOMA ACUMINATUM

This Paper introduces a microstrip applicator for treatment of cervical erosion and cervical condyloma acuminatum with microwave tissue coagulationmethod. The principle of the applicator,the theoretical calculation of near field distribution of the applicator covered with a lossy dielectric, the experimental resultsfor its performance of the impedance matching and for microwave leakage energyare reported. Finally,the therapeutic method and effects are presented.

Expression of MDM2 Protein and mRNA in Condyloma Acuminata

In order to investigate the role of murine double minute 2 (MDM2) in cell proliferation and carcinogenesis in condyloma acuminatum (CA), immunohistochemistry and in situ hybridization were used to detect the expression of MDM2 protein and mRNA in normal skin and skin lesions of CA of vulva. PCR was also used to detect HPV types. The results showed that in 32 observed CA specimens, the expression of MDM2 protein and mRNA was detected in 18(56.25 %)and 22(68.75 %)respectively, while the co-expression of MDM2 protein and mRNA was found in 14. PCR results revealed that HPV6/11 and HPV16/18 subtypes were shown in 28(87.5 %)and 4 (12.5 %)respectively out of 32 CA specimens. Out of the 18 positive specimens expressing MDM2 protein, HPV6/11 subtypes were shown in 15 and HPV16/18 subtypes in 3. In 22 positive specimens expressing MDM2 mRNA, HPV6/11 subtypes were shown in 18 and HPV16/18 subtypes in 4. No expression of MDM2 protein and MDM2 mRNA was observed in normal skin. Our study indicated that the overexpression of MDM2 might be involved in malignant proliferation and carcinogenesis of CA.

The Efficacy of Podophyllin Cautery Added to Surgical Excision for Eradication of Vulvar Condylomata Accuminata;Randomized Controlled Trial

Context: Condyloma acuminatum is a common morbidity caused by human papillomavirus infection. Objective: To compare the recurrence rate after surgical excision with or without podophyllin cautery. Design, Setting, Participants: Sixty women were enrolled in a study that was conducted in Cairo from Jan-2017 to Mar-2018. Interventions: All women received the same preparations. After randomization;in the study group (N = 30), podophyllin cautery was added to surgical removal in the follow-up period. In the control group (N = 30), only surgical removal was used. Main Outcome Measure: The main outcome measure was the estimation of the recurrence of the lesion during the follow-up period. The secondary outcome measures were the incidence of adverse events. Results: Both groups were comparable (p-value > 0.05) with regard to the age and BMI. Recurrence was significantly lower (P = 0.001) in the study group than the control group. In the study group, five cases (17.2%) have recurrence whereas, in the control group, 18 (60.0%) had suffered recurrence. The ARR was 42.8% (CI 95%: 18% - 60.9%) with RR of 0.29 (CI 95%: 0.12% - 0.67%) and NNT2 (CI 95%: 6% - 2%). During the follow-up period, 19 cases (65.5%) of the study group experienced pain versus only eight cases (26.7%) in the control group (p = 0.003). However, the development of scars was less in the study group 7 (24.1%) than in the control group 19 (63.3%), (p = 0.002). Conclusion: Podophyllin cautery, when added to surgical removal, is effective in decreasing the incidence recurrence in cases with condylomata acuminata.

Condyloma(朗玛)研究——克尤瑞坦(医用生物凝胶敷料)联合外用盐酸氨酮戊酸散光动力治疗尖锐湿疣临床效果研究观察

目的 探讨克尤瑞坦联合外用盐酸氨酮戊酸散(aminolevulinic acid,ALA)光动力疗法治疗尖锐湿疣临床疗效。方法 选取2020年1月至2021年1月收治的湿疣患者142例作研究对象,随机分成治疗组、对照组,各71例,治疗组ALA光动力治疗后联合克尤瑞坦治疗,对照组ALA光动力治疗后联合α干扰素治疗,比较两组治疗前后临床疗效、疣体直径与疣体脱落率、HPV病毒清除率。结果 (1)对照组总有效率87.32%,治疗组95.77%,治疗组总有效率显著高于对照组(P <0.05)。(2)治疗组治疗后4、6、8、12周时疣体直径改善均显著高于对照组(P <0.05)。(3)治疗组总复发率7.04%,对照组19.72%,治疗组总复发率较对照组显著偏低(P <0.05)。(4)治疗组总不良反应发生率(4.23%)较对照组(19.72%)显著偏低(P <0.05)。(5)治疗组治疗6、8周时疣体脱落率(80.00%和95.90%)较对照组(64.43%和86.60%)显著更高(P <0.05)。(6)治疗组治疗炎性因子表达水平明显低于对照组(P <0.05)。结论 克尤瑞坦联合ALA光动力疗法能有效改善尖锐湿疣患者的临床疗效,降低疣体直径,提高疣体脱落率和HPV病毒清除率,降低HPV感染风险和复发率,缩短病程周期,有较好的临床应用于推广价值。

尖锐湿疣患者恐惧疾病进展现状及影响因素

目的探讨尖锐湿疣患者恐惧疾病进展现状及相关影响因素。方法采用便利抽样法,选取从2021年12月至2023年1月在郑州大学第一附属医院确诊为尖锐湿疣的263例患者。采用患者一般资料调查表、中文版恐惧疾病进展简化量表、中文版痛苦表露指数量表、心理弹性量表简表等进行调查,使用SPSS 25.0中Spearman秩相关分析和多重线性回归分析影响尖锐湿疣患者恐惧疾病进展的相关因素。结果患者恐惧疾病进展得分为(38.21±5.64)分,心理弹性得分为[73.00(65.00,77.00)]分,自我表露得分为[35.00(31.00,39.00)]分,患者的心理弹性水平、自我表露水平、确诊时间、是否首次治疗是影响患者恐惧疾病进展水平的相关因素。结论关注病程较长的患者,在首次治疗时进行干预,促进患者自我表露,提高心理弹性水平,降低患者疾病进展的恐惧感。

尖锐湿疣患者光动力学治疗后短期复发风险预测模型的构建与验证

目的构建尖锐湿疣患者光动力学治疗后短期复发的风险预测模型,并验证其效果。方法选择2022年1月-2024年2月我科行光动力学治疗的尖锐湿疣患者153例作为调查对象,通过电子病历系统完成一般资料收集,以Logistic回归分析法确定尖锐湿疣患者光动力学治疗后短期复发的危险因素,构建风险预测模型并验证,提出相应的护理策略。结果随访6个月,153例尖锐湿疣患者中41例(26.79%)出现复发,列为复发组;112例(73.21%)患者治愈,列为治愈组;Logistic回归分析结果显示,性伴侣数量、是否使用安全套、皮损数目、血清白介素-12(IL-12)、白介素-2(IL-2)、转化生长因子-β1(TGF-β1)水平均为尖锐湿疣患者光动力学治疗后短期复发的独立危险因素(P<0.05)。风险预测模型Hosmer-Lemeshow拟合优度结果显示,χ^(2)=4.874,P=0.713,C-index为0.741,ROC曲线下面积为0.776(95%CI:0.602~0.892),灵敏度为0.828,特异度为0.848,临界值为0.329,预测准确率为80.64%。结论尖锐湿疣患者光动力学治疗后短期复发风险较高,构建的风险预测模型具有较好的拟合优度和区分能力,准确率较高,可为患者短期复发的早期筛查和识别提供依据。

男性尖锐湿疣患者组织人乳头瘤病毒基因型的感染情况

目的:分析男性尖锐湿疣患者组织人乳头瘤病毒(HPV)基因型的感染情况。方法:选择2021年12月—2023年12月于青岛市第三人民医院就诊的199例男性尖锐湿疣患者,采集疣体组织,采用聚合酶链式反应(PCR)-反向斑点杂交法检测HPV感染情况,统计男性尖锐湿疣患者HPV感染基因分型,不同感染状态、类型、年龄段患者HPV感染情况。结果:199例男性尖锐湿疣患者HPV感染基因分型中以6亚型和11亚型为主,分别占33.00%、19.19%。低危型、高危型、混合型HPV感染占比分别为53.77%、21.11%、25.13%,以低危型为主。单一感染、二重感染、多重感染占比分别为74.87%、11.56%、13.57%,以单一感染为主。<20岁、20~29岁、30~39岁、40~49岁、50~59岁、≥60岁患者的HPV感染占比分别为6.03%、33.67%、25.63%、19.60%、13.57%、1.51%,感染人群以20~29岁为主。结论:男性尖锐湿疣患者HPV感染基因分型以6亚型和11亚型为主,以低危型、单一感染居多,患病人群年龄集中于20~29岁,临床应加以重视,积极做好男性尖锐湿疣HPV感染防治工作。