Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize

Single-nucleotide polymorphisms contribute to phenotypic diversity in maize. Creation and functional annotation of point mutations has been limited by the low efficiency of conventional methods based on random mutation. An efficient tool for generating targeted single-base mutations is desirable for both functional genomics and precise genetic improvement. The objective of this study was to test the efficiency of targeted C-to-T base editing of two non-allelic acetolactate synthase(ALS) in generating sulfonylurea herbicide-resistant mutants. A CRISPR/Cas9 nickase-cytidine deaminase fused with uracil DNA glycosylase inhibitor(UGI) was employed to achieve targeted conversion of cytosine to thymine in ZmALS1 and ZmALS2. Both protoplasts and recovered mutant plants showed the activity of the cytosine base editor, with an in vivo efficiency of up to 13.8%. Transgene-free edited plants harboring a homozygous ZmALS1 mutation or a ZmALS1 and ZmALS2 double mutation were tested for their resistance at a dose of up to 15-fold the recommended limit of chlorsulfuron, a sulfonylurea herbicide widely used in agriculture. Targeted base editing of C-to-T per se and a phenotype verified in the generated mutants demonstrates the power of base editing in precise maize breeding.

A Truncated F-Box Protein Confers the Dwarfism in Cucumber

Dwarfism is an important plant architecture trait in crop breeding（Peng et al.,1999;Sasaki el al.,2002）.In cucurbits.the compact plant type was proposed to develop new varieties for the once-over mechanical harvest for concentrated fruit set and higher densities（Li et al.,2011;Mondal et al.,2011）.

bHLH104 confers tolerance to cadmium stress in Arabidopsis thaliana

Cd is a non-essential heavy metal that is toxic to both plants and animals. Here, we reveal that the transcription factor bHLH104 positively regulates Cd tolerance in Arabidopsis thaliana. We show that Fe deficiency-responsive genes were induced by Cd treat- ment, and that their upregulation was suppressed in bhlh104 loss-of-function mutants, but enhanced upon overexpression of bHLH104. Correspondingly, the bhlh104 mutants displayed sensitivity to Cd stress, whereas plants overexpressing bHLH104 exhibited enhanced Cd tolerance. Further analysis suggested that bHLH104 positivelyregulates four heavy metal detoxification-associated genes, IREG2, A4TP3, HA4A3 and NAS4, which play roles in Cd sequestration and tolerance. The bHLH104 overexpres- sion plants accumulated high levels of Cd in the root but low levels of Cd in the shoot, which might contribute to the Cd tolerance in those lines. The present study thus points to bHLH104 as a potentially useful tool for genetic engineering of plants with enhanced Cd tolerance.

QTL editing confers opposing yield performance in different rice varieties

Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci（QTLs）. In the past decade, many yield-contributing QTLs have been identified in crops.However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas_9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds.

口译课程思政中Mock Conference的作用

近年来,课程思政受到了越来越广泛的关注。本文从学生反馈的角度入手,探讨Mock Conference在口译课程思政中发挥的作用。本文通过对学生及任课教师的访谈,发现Mock Conference能够促进学生在语言能力、习惯态度、心理素质、职业素养、价值观塑造与意识培养等方面的提升,对于思政培养有积极作用。本研究为口译课堂中的思政建设提供了一定的借鉴,也为口译课程思政的实现提供了更多思路。

A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

We previously reported a spotted-leaf mutant pelota（originally termed HM_（47）） in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the mapbased cloning of the causal gene OsPELOTA（originally termed spl^（HM47））. We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1（eRF_1）, shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane.OsPELOTA is constitutively expressed in roots, leaves,sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.

Shaping global health promotion:a comprehensive analysis of the 10 Global Conferences on Health Promotion Conferences(1986-2021)

Objective This study analyzed how the 10 Global Conferences on Health Promotion have played a significant role in shaping and promoting a worldwide consensus and actions on health promotion,effectively addressing diverse health challenges that evolved over different periods.Methods The textual analysis method was used in this study and text encoding was conducted to systematically examine the declarations and reports presented by the 10 Global Conferences on Health Promotion held during 1986-2021.We summarized the themes and key achievements,and key vocabulary in the conference declarations was extracted and analyzed to construct the global health promotion consensus and actions.Results The fundamental principles of the conferences are to foster consensus and initiate actions in the realm of health promotion on a global scale.The primary purpose and goal are to promote health from regional to global.Significantly,our findings highlight a transition in the primary actors driving health promotion.It underscores a shift in health promotion from being driven primarily by organizations like the World Health Organization,governments,and international bodies,to a more inclusive approach involving non-governmental organizations and the general public.This development implies that health promotion has evolved into a collective global endeavor,demanding the proactive involvement of various stakeholders,and forging new alliances in public health.Meanwhile,the coronavirus disease 2019(COVID-19)pandemic has further shaped the landscape of health promotion,underscoring the need for intensified focus on areas including disease prevention,health education,and the integration of digital health technologies,and emphasizing the importance of a multidimensional,responsive approach in public health initiatives.Conclusions Sustained collaboration and innovative strategies are pivotal to advancing health promotion globally.Countries,together with public and private entities,should intensify cooperation.Multisectoral collaboration among partners such as healthcare,education,social security,and the industry is vital for health promotion and achieving global health goals.

Method Verification and Validation of Hydralazine Hydrochloride: Spectrophotometric Analysis in Pure and Pharmaceutical Formulations

The new method proposed is based on the formation of hydralazine-Bromophenol blue ion pair simply and without further extraction or heating. The ion pair was prepared in the presence of pH 3 citrate buffer forming a yellow-colored chromogen. A new maximum UV-visible band formed at 416 nm. The color was stable for more than 10 hours and obeyed Beer’s Law over the concentration range of 10 - 50 µg/mL. The calculated molar absorptivity and Sandell’s sensitivity were 1.01 × 104 L∙mol−1∙cm−1 and 0.0514 µg/mL, respectively. The elements of method validation stipulated by The International Conference on Harmonization [Q2 (R1)] were applied for hydralazine hydrochloride assay in pure and pharmaceutical tablet formulation. The average recoveries of the pure solution and the pharmaceutical formulation were 98.94% and 99.50%, respectively. The results were statistically compared by F-test, which indicates that the method can be precise and repeatable for both pure and pharmaceutical solutions. The method was found to be accurate, reproducible, and cost-effective, and validated for the assay of hydralazine in terms of the routine quality control.

Simultaneous Interpreting Output Assessment of Earnings Conference Call: Case Analysis of Tencent 2022 Q3 Result Announcement

Quality in simultaneous interpreting is a frequently discussed concept. In the enterprise setting, earnings conference call remains a rarely explored field. This thesis offers a descriptive study on assessing interpreting quality from perspectives of fidelity, fluency, and appropriacy. As the corpus, Tencent 2022 Third Quarter Result Announcement provides an ideal transcript to the author to conduct its analysis. Interpreting is frequently done without bearing in mind the multitude of factors that can affect the quality of interpreting. Drawing a conclusion that the interpreter does make a lot of omissions, pauses and hesitations posing a negative effect on the fidelity, fluency and accuracy of the interpreting, the present author suggests that more preparation should be done for improving performance, such as terminologies, company background information, a reasonable speech rate, good image and acoustic quality, and so on.

Epigenetic regulation of paired NLR receptors confers durable disease resistance without yield penalty in rice

Subject Code:C14 With the support by the National Natural Science Foundation of China,the research team led by Prof.He Zuhua(何祖华)at the National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research,Shanghai Institute of Plant Physiology&Ecology,Chinese Academy of Sciences,

A natural allele of Bsr-d1 in rice confers broad-spectrum blast resistance

Subject Code:C14With support from the National Natural Science Foundation of China,a collaborative research group led by Prof.Chen Xuewei(陈学伟)of the Rice Research Institute,Sichuan Agricultural University,published an article entitled“A natural allele of a transcription factor in rice confers broad-spectrum

President Xi Jinping Confers “Five Principles Of Peaceful Coexistence Friendship Award” on Indian Friendship Groups and Individuals

On September 19,2014 during a state visit to India,President Xi Jinping attended a ceremony held by the CPAFFC in New Delhi to confer the"Five Principles of Peaceful Coexistence Friendship Award"on various recipients.The year of 2014 marks the 60th anniversary of the enunciation of the Five Principles of Peaceful Coexistence,namely,mutual respect for sovereignty and territorial integrity。

CPAFFC Confers Title of Friendship Ambassador on SCA President Dr. Thomas Wagner

On the evening of March 7, the CPAFFC held a grand ceremony to confer the title of Friendship Ambassador on Dr. Thomas Wagner, president of the Switzerland-China Association (SCA). Chen Haosu, president of the CPAFFC; Jing Dunquan,

Summary of the 10th Conference on Magnetically Confined Fusion Theory and Simulation(CMCFTS)

This paper gives a summary of the organization and the presentations delivered at the 10th Conference on Magnetically Confined Fusion Theory and Simulation(CMCFTS)held in Zhuhai,China,from 28th to 31st October 2022.The conference focused on the latest progress in the research of the magnetic confined fusion plasma theory and simulations,as well as the largescale numerical simulation techniques developed in recent years.This conference is held both online and offline,with about 110 domestic participants from 18 institutes participating in the live conference,and the statistical data from the live broadcast platform indicated that the online conference attracted over 20000 views per day.A summary of the conference is given,and the history of the CMCFTS is presented.A brief introduction to the poster section is also included in this paper.

Video Conference System in Mixed Reality Using a Hololens

The mixed reality conference system proposed in this paper is a robust,real-time video conference application software that makes up for the simple interaction and lack of immersion and realism of traditional video conference,which realizes the entire process of holographic video conference from client to cloud to the client.This paper mainly focuses on designing and implementing a video conference system based on AI segmentation technology and mixed reality.Several mixed reality conference system components are discussed,including data collection,data transmission,processing,and mixed reality presentation.The data layer is mainly used for data collection,integration,and video and audio codecs.The network layer uses Web-RTC to realize peer-to-peer data communication.The data processing layer is the core part of the system,mainly for human video matting and human-computer interaction,which is the key to realizing mixed reality conferences and improving the interactive experience.The presentation layer explicitly includes the login interface of the mixed reality conference system,the presentation of real-time matting of human subjects,and the presentation objects.With the mixed reality conference system,conference participants in different places can see each other in real-time in their mixed reality scene and share presentation content and 3D models based on mixed reality technology to have a more interactive and immersive experience.

Differences between journal and conference in computer science:a bibliometric view based on Bayesian network

Purpose:This paper aims to investigate the differences between conference papers and journal papers in the field of computer science based on Bayesian network.Design/methodology/approach:This paper investigated the differences between conference papers and journal papers in the field of computer science based on Bayesian network,a knowledge-representative framework that can model relationships among all variables in the network.We defined the variables required for Bayesian networks modeling,calculated the values of each variable based Aminer dataset(a literature data set in the field of computer science),learned the Bayesian network and derived some findings based on network inference.Findings:The study found that conferences are more attractive to senior scholars,the academic impact of conference papers is slightly higher than journal papers,and it is uncertain whether conference papers are more innovative than journal papers.Research limitations:The study was limited to the field of computer science and employed Aminer dataset as the sample.Further studies involving more diverse datasets and different fields could provide a more complete picture of the matter.Practical implications:By demonstrating that Bayesian networks can effectively analyze issues in Scientometrics,the study offers valuable insights that may enhance researchers’understanding of the differences between journal and conference in computer science.Originality/value:Academic conferences play a crucial role in facilitating scholarly exchange and knowledge dissemination within the field of computer science.Several studies have been conducted to examine the distinctions between conference papers and journal papers in terms of various factors,such as authors,citations,h-index and others.Those studies were carried out from different(independent)perspectives,lacking a systematic examination of the connections and interactions between multiple perspectives.This paper supplements this deficiency based on Bayesian network modeling.

为全面建设社会主义现代化国家贡献标准化力量

Tian Shihong,Vice Minister of SAMR and Administrator of SAC,gave a work report at the annual National Standardization Work Conference.The report made an overview of the standardization work in 2022 and the achievements in the past five years,showcased how to achieve high-quality standardization development based on the guiding principles of the 20th National Congress of the Communist Party of China(CPC),and introduced the standardization priorities in 2023.Here are some highlights of the report.

异位甲状腺

1临床资料患者,女,36岁,以咽部异物感3个月,呼气困难半个月为主诉入院。患者3个月前无明显诱因出现咽部异物感等不适,未在意。半个月前开始出现平卧时憋气、呼吸不畅,坐位时呼吸困难可缓解,伴说话含物音及吞咽疼痛,无声嘶。就诊后,可见舌根半球形肿块,表面光滑,以舌根肿块收入院。

舌根肿瘤

患者，女，53岁，因咽异物感1个月入院。患者1个月前无明显诱因出现咽部左侧异物感，言语口齿清晰，无咽痛，无口腔异味，无痰中带血，无吞咽困难。曾于外院口腔科及耳鼻咽喉头颈外科就诊，口腔与咽喉部未见明显异常，诊为“咽喉炎”并给予对症药物治疗。治疗后无改菩，于我院就诊，舌根左侧可触及质硬肿块，以“舌根肿瘤”收入院。