A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenaz...A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenazine. The optimal conditions for laccase in organic media areT=55°C, pH=6.5, 1.0×10?2mol/L OPDA, 1.25 mL ethanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol, 1,4-dioxane and acetone media were 0.44–19.33, 0.11–20.85, 0.38–21.05 U with the detection limit of 0.088, 0.022, 0.076 U, respectively. The proposed method has been applied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported.展开更多
The modernization of water conservancy project management is a complicated engineering system involving a management system, a management method, management personnel, the exertion of social, economic, and ecological ...The modernization of water conservancy project management is a complicated engineering system involving a management system, a management method, management personnel, the exertion of social, economic, and ecological effects, and so on. However, indices for evaluating the modernization of water conservancy project management are usually unobtainable in practical applications. Conducting appropriate extension of the classical rough set theory and then applying it to an incomplete information system are the key to the application of the rough set theory Based on analysis of some extended rough set models in incomplete information systems, a rough set model based on the θ-improved limited tolerance relation is put forward. At the same time, upper approximation and lower approximation are defined under this improved relation. According to the evaluation index system and management practices, the threshold for θ is defined. An example study indicates the practicability and maneuverability of the model.展开更多
As a kind of parallel storage system, RAID5 has been becoming a popular solution for providing better performance at low cost and without sacrificing much redundant data, its main disadvantage is poor performance. It ...As a kind of parallel storage system, RAID5 has been becoming a popular solution for providing better performance at low cost and without sacrificing much redundant data, its main disadvantage is poor performance. It is a general method to improve RAID5 performance by using cache. However, it often happens that the data is not hit in cache, in this case, the RAID5 performance also will be very poor. The method based on combination of disk I/O operations has been proposed for impriving the I/O response time through reducing the number of low-level operations. According to theoretic analysis and experimental test, we find that RAID5 access time and data transfer rate could be largely improved than conventional method.展开更多
文摘A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenazine. The optimal conditions for laccase in organic media areT=55°C, pH=6.5, 1.0×10?2mol/L OPDA, 1.25 mL ethanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol, 1,4-dioxane and acetone media were 0.44–19.33, 0.11–20.85, 0.38–21.05 U with the detection limit of 0.088, 0.022, 0.076 U, respectively. The proposed method has been applied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported.
基金supported by the Fundamental Research Funds for the Central Universities(Grant No.2013B102008)the Open Fund of the Yellow River Committee(Grant No.2011535012)
文摘The modernization of water conservancy project management is a complicated engineering system involving a management system, a management method, management personnel, the exertion of social, economic, and ecological effects, and so on. However, indices for evaluating the modernization of water conservancy project management are usually unobtainable in practical applications. Conducting appropriate extension of the classical rough set theory and then applying it to an incomplete information system are the key to the application of the rough set theory Based on analysis of some extended rough set models in incomplete information systems, a rough set model based on the θ-improved limited tolerance relation is put forward. At the same time, upper approximation and lower approximation are defined under this improved relation. According to the evaluation index system and management practices, the threshold for θ is defined. An example study indicates the practicability and maneuverability of the model.
基金Supported by the National Natural Science Foundation of China under grant6 97730 46 and(6 99730 17) and Defense Advanced Resea
文摘As a kind of parallel storage system, RAID5 has been becoming a popular solution for providing better performance at low cost and without sacrificing much redundant data, its main disadvantage is poor performance. It is a general method to improve RAID5 performance by using cache. However, it often happens that the data is not hit in cache, in this case, the RAID5 performance also will be very poor. The method based on combination of disk I/O operations has been proposed for impriving the I/O response time through reducing the number of low-level operations. According to theoretic analysis and experimental test, we find that RAID5 access time and data transfer rate could be largely improved than conventional method.