期刊文献+
共找到267篇文章
< 1 2 14 >
每页显示 20 50 100
Implement Constitutional Principles to Uncover Tort Cases——An interview with Wang Zhenchuan, deputy chief procurator of the Supreme People's Procuratorate
1
作者 STAFF REPORTER CHEN CHUN’AN 《The Journal of Human Rights》 2005年第2期8-12,共5页
EDITOR'S NOTE: Since May 2004, the people's procuratorates in China have concentrated their efforts on uncovering cases of encroaching upon human rights by government functionaries by abusing their power. The ... EDITOR'S NOTE: Since May 2004, the people's procuratorates in China have concentrated their efforts on uncovering cases of encroaching upon human rights by government functionaries by abusing their power. The move has aroused extensive attention. On this, our staff reporter interviewed Deputy Chief Procurator Wang Zhenchuan, who is in charge of the campaign against human rights violations. 展开更多
关键词 over An interview with Wang Zhenchuan Implement constitutional Principles to Uncover Tort Cases deputy chief procurator of the supreme People’s Procuratorate WANG
下载PDF
基于Seam的企业客户服务平台设计与实现 被引量:1
2
作者 赵小平 薛惠锋 《电子设计工程》 2012年第20期10-12,共3页
为了提高企业对客户信息化服务水平和整体运作效率,减轻工作人员的劳动强度,提升服务品质,全面降低企业运作成本,设计了一种基于Web的客户服务平台。重点研究了Seam的系统模型以及其总体架构、业务流程、功能模块和物理结构。应用结果表... 为了提高企业对客户信息化服务水平和整体运作效率,减轻工作人员的劳动强度,提升服务品质,全面降低企业运作成本,设计了一种基于Web的客户服务平台。重点研究了Seam的系统模型以及其总体架构、业务流程、功能模块和物理结构。应用结果表明,该方案可有效地集成和管理实现企业客户服务信息管理系统的网络化,系统实施之后,企业客户服务的工作效率得到了明显的提高。 展开更多
关键词 seam 信息支持系统 系统架构 服务平台 B/s模式
下载PDF
基于Seam的城市供水管理系统的设计与实现
3
作者 姚小明 刘耀军 郭银章 《计算机工程与设计》 CSCD 北大核心 2009年第21期5013-5015,5024,共4页
在基于JavaEE(J2EE)软件开发方面,目前流行的软件架构方式是Spring+Hibernate+Struts,它的缺点是开发较慢,难于维护。通过引入JBoss Seam作为主体框架,用于开发基于三层B/S模式的城市供水管理系统,利用JBoss Seam所提供的注释和双向注入... 在基于JavaEE(J2EE)软件开发方面,目前流行的软件架构方式是Spring+Hibernate+Struts,它的缺点是开发较慢,难于维护。通过引入JBoss Seam作为主体框架,用于开发基于三层B/S模式的城市供水管理系统,利用JBoss Seam所提供的注释和双向注入,组件的状态管理,以及所集成的大量框架和服务等各类功能,能够加快系统的开发、降低开发及维护难度。系统持久层使用JBoss Seam所集成的Hibernate,表示层使用Seam所推荐的JSF框架、支持JSF的Facelets模板框架以及内置支持Ajax的RichFaces框架,能够快速开发出以构件为中心、模板化的、体现Web2.0风格的页面。 展开更多
关键词 JAVAEE JBOss seam 城市供水管理系统 构件 三层B/s模式
下载PDF
Statistic constitutive equation of top-coal damage for fully mechanized coal face with sublevel caving 被引量:2
4
作者 翟新献 《Journal of Coal Science & Engineering(China)》 2008年第1期6-11,共6页
Under the action of abutment pressure in front of fully mechanized coal face with sublevel caving(CFSC),top-coal over CFSC deformed.In the process of whole de- formation of top-coal,it changed from continuum elastic m... Under the action of abutment pressure in front of fully mechanized coal face with sublevel caving(CFSC),top-coal over CFSC deformed.In the process of whole de- formation of top-coal,it changed from continuum elastic mass to non-continuum plastic mass contained fissures,become a loose body.According to its bearing characteristics and mechanical properties,top-coal mass can be divided into four deformation zones along the winning direction of CFSC,i.e.initial stress zone,elastic zone,plastic zone and loose zone.Top-coal in plastic zone located in the post-peak zone of the stress-strain curve for top-coal.With equivalent strain principle of damage mechanics and mathemati- cal theory of statistic,combining the movement law of top-coal,set up a constitutive equa- tion with damage statistics for top-coal in different position in CFSC.The equation illus- trated the mathematical relationship among top-coal bearing capacity,horizontal confining pressure along the winning direction of CFSC and mechanical properties of top-coal mate- rial.The conclusions not only provide a basis for numerical computer simulations on damage deformation and failure mechanism for top-coal,but also further promote the ap- plication of damage mechanics in CFSC. 展开更多
关键词 coal seam fully mechanized coal face with sublevel caving (CFsC) top-coaldamage constitutive equation
下载PDF
A unified constitutive model for multiphase precipitation and multi-stage creep ageing behavior of Al−Li−S4 alloy 被引量:2
5
作者 He LI Li-hua ZHAN +5 位作者 Ming-hui HUANG Xing ZHAO Chang ZHOU Li-bin HU Zheng-gen HU De-bo LIU 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第5期1217-1234,共18页
A unified constitutive model is presented to predict the recently observed“multi-stage”creep behavior of Al−Li−S4 alloy.The corresponding microstructural variables related to the yield strength and creep deformation... A unified constitutive model is presented to predict the recently observed“multi-stage”creep behavior of Al−Li−S4 alloy.The corresponding microstructural variables related to the yield strength and creep deformation of the alloy during the creep ageing process,including dislocations and multiple precipitates,have been characterized in detail by X-ray diffraction(XRD)and transmission electron microscopy(TEM).For the yield strength,the model considers the multiphase strengthening behavior of the alloy based on strengthening mechanisms,which includes shearable T1 precipitate strengthening,non-shearable T1 precipitate strengthening andθ′precipitate strengthening.Based on creep deformation mechanism,the“multi-stage”creep behavior of the alloy is predicted by introducing the effects of interacting microstructural variables,including the radius of multiple precipitates,dislocation density and solute concentration,into the creep stress−strain model.It is concluded that the results calculated by the model are in a good agreement with the experimental data,which validates the proposed model. 展开更多
关键词 constitutive modelling Al−Li−s4 alloy creep age forming MICROsTRUCTUREs numerical algorithms
下载PDF
Fecal-associated microbiome differences between phlegm-dampness constitution and balanced constitution 被引量:1
6
作者 Yini Li Pengfei Zhao +5 位作者 Yunan Zhang Jianhua Zhen Lu Zhao Yanan Cai Qingyi Lu Guangrui Huang 《Journal of Traditional Chinese Medical Sciences》 CAS 2022年第3期257-266,共10页
Objective: This study aimed to explore the structural and functional characteristics of the fecalassociated microbiome(FAM) between the phlegm-dampness constitution(PDC) and balanced constitution(BC), and to screen th... Objective: This study aimed to explore the structural and functional characteristics of the fecalassociated microbiome(FAM) between the phlegm-dampness constitution(PDC) and balanced constitution(BC), and to screen the related specific operational taxonomic unit(OTU) biomarkers.Methods: This was a cross-sectional study. After strictly identifying the constitution of subjects, their clinical index was recorded and counted. Fecal samples were collected for 16S rDNA sequencing. Alpha diversity, beta diversity, and the relative abundance of dominant bacterial taxa were used to describe the FAM structure, and the Wilcoxon rank-sum test, Metagenome Seq, and linear discriminant analysis effect size(LEf Se) were used to screen specific bacterial taxa. Specific OTUs were screened to construct receiver operating characteristic(ROC) curves.Results: Thirty-two subjects were enrolled, including 22 subjects with BC and 10 subjects with PDC.There were significant differences in cold preference, levels of aspartate transaminase, β2-microglobulin,and creatine kinase MB, and alpha diversity indices(Shannon and Shannoneven) between the two groups. In principal coordinate analysis by abund-jaccard distance measure and partial least squares discriminant analysis, bacterial communities clustered separately between the two groups. Furthermore,based on Metagenome Seq, LEf Se, and the Wilcoxon rank-sum test, a total of 43, 18, and 130 OTUs were differentially distributed between BC group and PDC group, respectively, and OTU200, OTU133, and OTU353 were screened when P≤.01. The area under the ROC curve constructed from the 3 selected OTUs was 0.93.Conclusion: The FAM structure and related functional characteristics of the PDC group differed from those of the BC group. In particular, OTU200, OTU133, and OTU353 can be used as unique markers of PDC to assist clinical diagnosis. 展开更多
关键词 Fecal-associated microbiome Traditional Chinese medicine constitution Phlegm-dampness constitution Balanced constitution Biomarkers 16s rDNA Metabolic diseases Intestinal flora
下载PDF
A GENERAL CONSTITUTIVE RELATION FOR FATIGUE CRACK GROWTH ANALYSIS OF METAL STRUCTURES 被引量:27
7
作者 W.C. Cui and X.P. HuangSchool of Naval Architecture & Ocean Engineering, Shanghai Jiaotong University, Shanghai 200030, China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2003年第5期342-354,共13页
Crack growth rate curves are the fundamental material property for metal structures under fatigue loading. Although there are many crack growth rate curves available in the literature, few of them showed the capabilit... Crack growth rate curves are the fundamental material property for metal structures under fatigue loading. Although there are many crack growth rate curves available in the literature, few of them showed the capability to explain various special phenomena observed in tests. A modified constitutive relation recently proposed by McEvily and his co-workers showed very promising capability. This modified constitutive relation is further generalized by (1) introducing an unstable fracture condition; (2) defining a virtual strength to replace the yield stress; and (3) defining an overload and underload parameter. The performances of this general constitutive relation for fatigue crack growth is extensively studied and it is found that this general constitutive relation is able to explain various phenomena observed with particular strong capability on load sequence effect. 展开更多
关键词 metal fatigue s-N curve crack growth rate general constitutive relation mean stress effect load sequence effect
下载PDF
Geomechanics of subsidence above single and multi-seam coal mining 被引量:6
8
作者 A.M.Suchowerska Iwanec J.P.Carter J.P.Hambleton 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2016年第3期304-313,共10页
Accurate prediction of surface subsidence due to the extraction of underground coal seams is a significant challenge in geotechnical engineering. This task is further compounded by the growing trend for coal to be ext... Accurate prediction of surface subsidence due to the extraction of underground coal seams is a significant challenge in geotechnical engineering. This task is further compounded by the growing trend for coal to be extracted from seams either above or below previously extracted coal seams, a practice known as multiseam mining. In order to accurately predict the subsidence above single and multi-seam longwall panels using numerical methods, constitutive laws need to appropriately represent the mechanical behaviour of coal measure strata. The choice of the most appropriate model is not always straightforward. This paper compares predictions of surface subsidence obtained using the finite element method, considering a range of well-known constitutive models. The results show that more sophisticated and numerically taxing constitutive laws do not necessarily lead to more accurate predictions of subsidence when compared to field measurements. The advantages and limitations of using each particular constitutive law are discussed. A comparison of the numerical predictions and field measurements of surface subsidence is also provided. 展开更多
关键词 MiningCoalLongwall miningsubsidence Multi-seam mining constitutive modelling
下载PDF
Screening influencing factors of blood stasis constitution in traditional Chinese medicine 被引量:5
9
作者 ZHOU Xiaoying YANG Shengwen +3 位作者 OU Jintao WANG Zhuo WANG Guangrong LUO Yue 《Digital Chinese Medicine》 2022年第2期169-177,共9页
Objective To study the influencing factors of blood stasis constitution and provide a basis for treating blood stasis-related diseases by traditional Chinese medicine(TCM) constitution identification.Methods Data were... Objective To study the influencing factors of blood stasis constitution and provide a basis for treating blood stasis-related diseases by traditional Chinese medicine(TCM) constitution identification.Methods Data were collected using the self-developed TCM constitution identification platform based on B/S model by the project team. The obtained data were divided into blood stasis constitution and normal constitution groups. The differences of the categorical type influencing factors(gender, birth mode, feeding mode within four months of birth, family history, marital status, eating habits, sleeping habits, exercise habits, emotional state, stress situation, and living environment) and the quantitative type influencing factors(sleep time, age,and mother’s age at birth) on the constitution of the two groups were analyzed. In the singlefactor analysis, the Pearson’s chi-square test was selected for the categorical variable, and the independent sample t test and Mann-Whitney U nonparametric test were selected for the quantitative variables according to whether they conformed to the positive-terrestrial distribution;the binary logistic stepwise regression method was selected for the multi-factor analysis.Results The data of 318 cases were collected from the TCM composition identification platform, and 159 cases of blood stasis constitution were used as the experimental group and 159 cases of normal constitution were used as the control group. The Pearson’s chi-square test yielded significant differences(P < 0.05) in the effects of gender, pressure situation, family history, living environment, emotional state, exercise habits, and dietary habits on blood stasis constitution. The independent samples t test yielded differences in sleep duration between the blood stasis constitution and normal constitution populations(P < 0.05), which meant sleep duration of the blood stasis constitution population was less than that of the normal constitution population. The Mann-Whitney U nonparametric test results accepted the original hypothesis that there was no difference in the distribution of age and mother’s age at birth across constitution types(P > 0.05). Binary logistic regression analysis showed that gender, family history, marital status, living environment, exercise habits, and emotional state were risk factors for blood stasis constitution(P < 0.05).Conclusion Gender, family history, living environment, emotional state, and exercise habits were significant influencing factors of blood stasis constitution. Blood stasis constitution populations can pay more attention to these influencing factors in their daily life for the prevention and reconciliation of blood stasis constitution. 展开更多
关键词 Blood stasis constitution screening influencing factors Pearson’s chi-square test Nonparametric test Binary logistic stepwise regression
下载PDF
A 3-D Visco-Hyperelastic Constitutive Model for Rubber with Damage for Finite Element Simulation
10
作者 Ala Tabiei Suraush Khambati 《Computer Modeling in Engineering & Sciences》 SCIE EI 2015年第5期25-45,共21页
A constitutive model to describe the behavior of rubber from low to high strain rates is presented.For loading,the primary hyperelastic behavior is characterized by the six parameter Ogden’s strain-energy potential o... A constitutive model to describe the behavior of rubber from low to high strain rates is presented.For loading,the primary hyperelastic behavior is characterized by the six parameter Ogden’s strain-energy potential of the third order.The rate-dependence is captured by the nonlinear second order BKZ model using another five parameters,having two relaxation times.For unloading,a single parameter model has been presented to define Hysteresis or continuous damage,while Ogden’s two term model has been used to capture Mullin’s effect or discontinuous damage.Lastly,the Feng-Hallquist failure surface dictates the ultimate failure for element deletion.The proposed model can accurately predict the response of rubber using a limited set of experimental data.The model has been validated here for the case of rubber but can be extended to a wide range of polymers. 展开更多
关键词 HYPERELAsTICITY VIsCOELAsTICITY hysteresis Mullin’s effect RUBBER constitutIVE
下载PDF
China's Constitution:Guarantee for People's Rights
11
作者 XU CHONGDE Professor, Law School, Renmin University of China 《The Journal of Human Rights》 2003年第2期28-30,共3页
The Communist Party of China, which represents the fundamental interests of the broadest possible masses of the people, has always attached much importance to the rights of the people and the safeguard measures. As ea... The Communist Party of China, which represents the fundamental interests of the broadest possible masses of the people, has always attached much importance to the rights of the people and the safeguard measures. As early as in the New Democratic Revolution period, it adopted in Ruijin, Jiangxi Province, an Outline Constitution of the All-China Soviet Republic in January 1934. The document stipulated that all workers, peasants, soldiers and other labor populace and their families, irrespective of their gender, ethnic group or religion, are equal before the law of the Soviet and enjoy the right to work, supervision, education and the freedom 展开更多
关键词 for IT on as BE of China’s constitution into that HAVE
下载PDF
Three Series of Amendments to China's Constitution
12
《China's Foreign Trade》 1999年第4期46-46,共1页
关键词 Three series of Amendments to China’s constitution
下载PDF
基于改进T-S模糊模型的弧焊机器人轨迹实时跟踪
13
作者 姚江云 王宽田 +1 位作者 李旺昆 梁世华 《组合机床与自动化加工技术》 北大核心 2024年第9期147-152,共6页
针对弧焊机器人在实际作业中受到诸多不确定因素的影响,导致弧焊机器人焊接轨迹偏离预期轨迹的问题,提出一种基于改进T-S(Takagi-Sugeno)模糊模型的弧焊机器人轨迹实时跟踪方法。采用图像识别技术识别并定位焊缝,通过坐标转换,确定焊缝... 针对弧焊机器人在实际作业中受到诸多不确定因素的影响,导致弧焊机器人焊接轨迹偏离预期轨迹的问题,提出一种基于改进T-S(Takagi-Sugeno)模糊模型的弧焊机器人轨迹实时跟踪方法。采用图像识别技术识别并定位焊缝,通过坐标转换,确定焊缝中心线在机器人坐标系中的位置坐标。利用蚁群算法根据焊缝中心线在机器人坐标系中的坐标,搜索从起始位置到目标位置的最优焊接路径。设计了一种改进区间二型T-S模糊模型,通过视觉传感器实时获取新的焊缝信息,计算轨迹误差,以此为输入,利用改进T-S模糊模型对机器人的运动轨迹进行实时调整。实验结果表明,采用改进T-S模糊模型后,机器人轨迹跟踪离散度均降至1 mm以下,弧焊机器人轨迹与预期轨迹高度重合,比传统方法跟踪离散度大大降低。 展开更多
关键词 改进T-s模糊模型 弧焊机器人 焊缝识别与定位 轨迹规划 轨迹实时跟踪方法
下载PDF
Installed capacity of coal seam gas power generation exceeds 480 MW under SGCC's coverage
14
《Electricity》 2009年第4期51-,共1页
The journalist learned from the "National Gas Security Working Conference" held recently that the coal seam gas power generation has been rapidly developed in recent years.As of July 2009,within the SGCC'... The journalist learned from the "National Gas Security Working Conference" held recently that the coal seam gas power generation has been rapidly developed in recent years.As of July 2009,within the SGCC's business area,the power generation 展开更多
关键词 Installed capacity of coal seam gas power generation exceeds 480 MW under sGCC’s coverage
下载PDF
Forum on China's Move to Enshrine Human Rights in Constitution
15
作者 BY OUR STAFF REPORTER 《The Journal of Human Rights》 2004年第3期39-40,共2页
China Society for Human Rights Studies (CSHRS) called a forum of experts and scholars on March 15 to discuss the significance of getting human rights protection enshrined in China’s fundamental law—the Constitution.... China Society for Human Rights Studies (CSHRS) called a forum of experts and scholars on March 15 to discuss the significance of getting human rights protection enshrined in China’s fundamental law—the Constitution. Presided over by CSHRS President Zhou Jue, the forum was attended by CSHRS vice-presidents Yang Zhengquan and Dong Yunhu and more than 20 human rights experts and scholars from the 展开更多
关键词 Forum on China’s Move to Enshrine Human Rights in constitution
下载PDF
10 Finance Measures Constituted by the Party's Central Committee to Support Enterprise Reform
16
《中国中小企业》 1999年第12期4-5,共2页
ThecommissionerofthePoliticalBureauoftheCentralCommitteeoftheChineseCommunistParty,theVicePresidentoftheStat... ThecommissionerofthePoliticalBureauoftheCentralCommitteeoftheChineseCommunistParty,theVicePresidentoftheStateCouncil,theSecre... 展开更多
关键词 Finance Measures constituted by the Party’s Central Committee to support Enterprise Reform
下载PDF
煤层底板突水危险性的Fisher判别分析模型 被引量:58
17
作者 张文泉 张广鹏 +1 位作者 李伟 华祥 《煤炭学报》 EI CAS CSCD 北大核心 2013年第10期1831-1836,共6页
为准确评价底板突水危险性,考虑到不同影响因素对于评价方法的作用与影响不同,运用逐步判别法剔除了判别效果不显著的指标,选取了含水层富水性、水压、隔水层厚度、断层导水性、构造发育程度5项主要影响因素作为判别指标。根据以往突水... 为准确评价底板突水危险性,考虑到不同影响因素对于评价方法的作用与影响不同,运用逐步判别法剔除了判别效果不显著的指标,选取了含水层富水性、水压、隔水层厚度、断层导水性、构造发育程度5项主要影响因素作为判别指标。根据以往突水数据建立了底板突水危险性的Fisher判别分析模型,并绘制了判别空间中样本数据散点图。该模型通过了显著性检验,分类效果显著。对15组训练样本进行回代判别,误判率为0,通过距离判别法准确预测了测试样本的突水危险性,表明该模型在煤层底板突水危险性评价上具有较高的可信度和良好的实用性。 展开更多
关键词 煤层 底板 突水 FIsHER判别 逐步判别 距离判别
下载PDF
基于SEEC-YOLO v5s的散养蛋鸡日常行为识别与统计系统 被引量:2
18
作者 杨断利 王永胜 +3 位作者 陈辉 孙二东 王连增 宁炜 《农业机械学报》 EI CAS CSCD 北大核心 2023年第9期316-328,共13页
针对鸡只个体较小、个体间存在遮挡,对蛋鸡日常行为识别造成干扰的问题,提出了一种基于SEEC-YOLO v5s的蛋鸡日常行为识别方法。通过在YOLO v5s模型输出部分添加SEAM注意力模块、在特征融合部分引入显式视觉中心模块(EVCBlock),扩大了模... 针对鸡只个体较小、个体间存在遮挡,对蛋鸡日常行为识别造成干扰的问题,提出了一种基于SEEC-YOLO v5s的蛋鸡日常行为识别方法。通过在YOLO v5s模型输出部分添加SEAM注意力模块、在特征融合部分引入显式视觉中心模块(EVCBlock),扩大了模型的感受野,提高了模型对小个体遮挡情况下的目标识别能力,提升了模型对蛋鸡站立、采食、饮水、探索、啄羽和梳羽6种行为的识别精度。提出了一种基于视频帧数与视频帧率比值计算蛋鸡日常行为持续时间的统计方法,并对蛋鸡群体一天之中不同时间段及全天各行为变化规律进行了分析。将改进后的模型进行封装、打包,设计了蛋鸡日常行为智能识别与统计系统。试验结果表明,SEEC-YOLO v5s模型对6种行为识别的平均精度均值为84.65%,比YOLO v5s模型高2.34个百分点,对比Faster R-CNN、YOLO X-s、YOLO v4-tiny和YOLO v7-tiny模型,平均精度均值分别提高4.30、3.06、7.11、2.99个百分点。本文方法对蛋鸡的日常行为监测及健康状况分析提供了有效的支持,为智慧养殖提供了借鉴。 展开更多
关键词 蛋鸡 日常行为识别 seam模块 EVCBlock模块 YOLO v5s
下载PDF
C-S模型参数及对非接触爆炸仿真分析的影响研究 被引量:5
19
作者 陈志坚 袁建红 +1 位作者 赵耀 谭万俊 《振动与冲击》 EI CSCD 北大核心 2008年第6期60-63,117,共5页
依靠SHPB技术,建立了高强度船用钢的C-S模型。对承受冲击因子3.7水下非接触爆炸载荷的结构,基于流固耦合理论,利用6种C-S模型,分别建立动态有限元模型进行非接触爆炸数值仿真,研究了C-S模型参数对仿真结果的影响。研究指出:无论是通过... 依靠SHPB技术,建立了高强度船用钢的C-S模型。对承受冲击因子3.7水下非接触爆炸载荷的结构,基于流固耦合理论,利用6种C-S模型,分别建立动态有限元模型进行非接触爆炸数值仿真,研究了C-S模型参数对仿真结果的影响。研究指出:无论是通过冲击压缩试验还是冲击拉伸试验获得的C-S模型参数,均可获得满意的仿真分析精度;C-S模型参数正确与否,将严重影响结构抗爆性能评估结论的正确性。 展开更多
关键词 水下结构 非接触爆炸 数值仿真 C—s模型 高强度船用钢
下载PDF
丹参晚期胚胎蛋白基因SmLEA的克隆及表达分析 被引量:4
20
作者 刘丛玲 王喆之 《生物技术通报》 CAS CSCD 北大核心 2009年第5期80-84,共5页
对丹参EST序列进行Blast分析,发现一条序列与晚期胚胎丰富蛋白(late embryogenesis abundant)基因有较高的相似性,在此基础上设计引物,分别从cDNA和gDNA水平克隆到该基因的全长(Genbank注册号:AY725206),命名为SmLEA。该序列全长739 bp... 对丹参EST序列进行Blast分析,发现一条序列与晚期胚胎丰富蛋白(late embryogenesis abundant)基因有较高的相似性,在此基础上设计引物,分别从cDNA和gDNA水平克隆到该基因的全长(Genbank注册号:AY725206),命名为SmLEA。该序列全长739 bp,无内含子,包含1个长为495 bp的开放阅读框,编码164个氨基酸。序列比对结果显示,该序列与番茄的晚期胚胎丰富蛋白Lemmi9有较高的相似性(69%),推测该编码蛋白属于晚期胚胎蛋白LEA14家族成员。生物信息学显示,SmLEA所编码蛋白SmLEA的相对分子质量为17.34 kD,理论等电点为4.51,富含天冬氨酸及AS、IP、KV、VS、TIP肽段,定位于细胞质中,为稳定类蛋白。实时荧光定量PCR结果显示,该基因在丹参的根、茎、叶中均有表达,为组成型表达基因。 展开更多
关键词 晚期胚胎丰富蛋白 生物信息学 组成型基因 丹参
下载PDF
上一页 1 2 14 下一页 到第
使用帮助 返回顶部