Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.

Construction and characterization of a cDNA library from human liver tissue with chronic hepatitis B

Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.

An Examination on the Support of Library Information to the Construction of National Research Organizations

The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has high economic and political achievements,China’s cultural soft power is still in a weak position.Cultural soft power is of great significance in improving the country’s overall national strength and international competitiveness.Research organizations are an important part of the country’s soft power and are of great significance to the strengthening of national soft power.Research organizations are the“peripheral brain”in the decision-making process of the government.National government agencies need democratic science in making decisions,and the adopted policy methods must have positive and effective outcomes on the people.Therefore,the national government agencies cannot achieve this without the help of research organizations.This resulted in the development and rising construction of research organizations.With the comprehensive development and construction of research organizations,whilst making progress,has also exposed several shortcomings.The construction of China’s research organizations requires various supports;especially the intelligence agencies and access to library information,which has a unique advantage in promoting the construction of research organizations[1].This paper first introduces the definition,classification and characteristics of a research organization.It will also provide a preliminary understanding of research organizations.The paper will then highlight and analyze the problems encountered by the research organization.Finally,through the grasp of the advantages of the library and intelligence agencies,a solution is proposed.

Research on the Construction of Chinese Universities from Intelligent Library to Smart Library

The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart library.Taking Anhui University of Finance and Economics as the research object,first,based on the literature and data combined with questionnaire design and expert interview,data statistics was made on the development of smart library.Second,analytical hierarchy process(AHP)and big data analysis were applied to complete the construction of the smart library system.Third,based on the data analysis method,the problems in the construction of the smart library were discussed.The results showed that the Chinese smart library started late and developed rapidly after 2012;from information perception to smart service,the construction of smart library system had realized the transformation and upgrading of library construction;the key to solve the construction of smart library was to standardize library construction,realize resource co-construction and sharing,promote technological innovation,and strengthen the construction of smart librarians.To sum up,the construction of smart library is expected to make the library and librarians become the cooperative objects of teaching and scientific research in colleges and universities.

Based on the Construction of University Library and the Evaluation of Space Service Quality

In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the quality evaluation of university library by establishing the evaluation index of space service quality,and puts forward the ways to improve the service quality of University Library for reference.

The plight and Countermeasures brought to the constructions of library literature by effect of college enrollment

This text introduces the concept of local literature and collection range, explains the significance of literature collection and explores building programs of local university library. Currently, university library generally put a strong emphasis on collection of academic literature, but has less attention on literature collections which reflect the situation of university location, or even worse, simply don＇ t launch business in this field. For the university library, it is a great loss of literature information resources, as well as a great regret of library work. First, what is the local literature？ It is literature data which document a region＇ s past and present issues like politics, economy, culture, education, history, geography and other aspects like important characters and events, customs, folklore etc.

Development and construction of China's higher education libraries

Libraries in China's higher education institutions have been developing in keeping pace with the flourishing development of China's higher education. This article aims to make an introduction to the construction of China's higher education libraries, especially the recent three decades' achievements since China's reform and opening-up in 1978. In this article, the authors draw a general picture of the development of libraries in China's higher education institutions, covering such eight aspects as management, types and positioning,organizational structure and personnel, expenditure and buildings, reader service, building and sharing of resources as well as automation system.

Construction and screening of suppression subtractive hybridization library of renal cell carcinoma

Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.

生成式AI视域下智慧图书馆建设的关键路径

[目的/意义]智慧图书馆已经成为当今图书馆学界和业界最热门的主题之一。智慧图书馆建设的理论研究与实践工作已经取得了初步成效,生成式AI也在蓬勃发展的同时参与到各行各业的建设中。因此有必要立足当下发展,放眼远景目标,确定生成式AI参与智慧图书馆建设的策略。[方法/过程]本文从智慧图书馆空间升级、服务打造、业务重组、人才培养、体系建设5个方面阐明了生成式AI视域下智慧图书馆建设的关键路径。[结果/结论]关键路径研究将为生成式AI引入智慧图书馆下一阶段的建设工作提供借鉴与依据。

公安实践教学中数字化标本库的构建与应用

为推进信息化教学的发展,提出在公安实践教学中建设数字化标本库的构想。以虚拟仿真教学中的毒品数字化标本库构建与教学组织应用为例,探讨公安数字化标本库建设可持续发展的路径和内涵,提出适合公安特色数字化标本库内容和标准化建设的建议,采用资源利用、整合、共建等高效节约办法,注重数字化样本库和数据库同步开发使用的发展趋势。数字化标本库具有线上自学、线上线下教学、终身学习等优势,可启发公安教育智慧教学工作高质量发展。

文化自信赋能高校图书馆创新发展

文化自信是一个国家、一个民族发展中最基本、最深沉、最持久的力量,坚定文化自信是全面建设社会主义现代化强国、实现中华民族伟大复兴的时代需要。大学是引领社会发展的重要引擎,高校图书馆理应成为文化自信的践行者。文章在分析文化自信内涵及其与高校图书馆价值耦合的基础上,从理念创新、空间布局、资源禀赋、服务生态、书香校园、地域特色文化等方面提出基于文化自信提升的高校图书馆创新发展路径。

江苏职业教育教学资源库建设成效、特色与优化策略——基于2022年校级质量年报的分析

职业教育教学资源库是服务智慧化学习和终身学习的重要载体,对于高技能人才培养具有重要意义。以江苏省20所国家级“双高计划”院校为研究对象,发现江苏省高职院校在教学资源库建设方面取得多重成效,具有多元主体协同共建、多平台路径扩大供给、和强化经费投入基础保障的特色。面对职业教育数字化发展,需要推行“五大行动计划”,高质量建设多维立体教学资源;对接智慧教学模式需要,高水平打造数字化教学资源;主动利用信息技术,赋能职业教育“课堂革命”策略,提高职业教育教学资源库的建设质量和使用效果。

数字时代图书馆法治建设高质量发展的五维审思

图书馆是文化资源的储存库和提取库,担负着凝聚民族亲和力与团结力的历史使命。数字时代《公共图书馆法》及其相关法律为数字化法治化图书馆的设立、运行及服务等提供了规范依据,呈现出布局时空、内驱外引、业态迭新、为民服务的发展优势。2023年2月中共中央、国务院相继印发《质量强国建设纲要》,指出要大力推动图书馆、博物馆等公共文化场馆数字化发展,加快线上线下服务融合。图书馆所蕴含的文化、教育、开放、共享等价值理念成为人民实现和平与发展的精神品格,有助于保障人民公共利益权利,有助于满足人民群众对文化资源获取的美好期待,有助于团结全体人民智慧共建人类文明共同体。

药学专业学位研究生课程案例库建设——以现代分析技术与实践课程为例

专业学位研究生教育目标是培养高层次应用型专门人才。药学专业学位教育强调实践性和职业性,案例教学有利于培养研究生技术创新能力和解决实际问题能力。鉴于目前药学专业学位课程案例库建设严重不足的问题,为更好地实施案例教学,常州大学药学院开展研究生课程案例库的建设与实践。该文描述现代分析技术与实践课程案例库建设的三个阶段,依次为广泛收集案例、深度加工案例、科学编排案例,最终案例库以教材形式呈现。案例教材的应用实践取得良好的教学效果,以期为药学专业学位其他课程建设提供借鉴经验。

面向信息资源管理学科的话语体系构建思考

在国家推进构建哲学社会科学话语体系与图书情报与档案管理一级学科更名的背景下,进一步厘清学科更名对其话语体系的影响有助于推动学科发展、适应时代变化。文章首先采用文献调研方法梳理了信息资源管理学科话语体系的发展现状,对学科话语进行解构并阐述了核心概念和学科语境的变化过程,又以2012年-2022年间“北大核心”和“CSSCI”两大数据库的期刊论文为语料,通过构建语义网络等方法探索近十余年间图书情报与档案管理学科学术话语的状态与发展态势,进而探讨信息资源管理学科话语体系建构的基本结构与构建路径。

非学历继续教育数字化教学资源库的建设

随着继续教育的重要性日益凸显,如何有效地提供教学资源成为一个关键问题。首先,分析了非学历继续教育的现状和发展趋势,阐述了数字化教学资源库的意义和作用;接着,提出了非学历继续教育数字化教学资源库的建设理念与目标,以及资源库的内容构建和技术支撑。同时,也探讨了资源库的应用与推广以及评估与持续改进等方面的问题。为构建高效、便捷、个性化的非学历继续教育数字化教学资源库提供理论和实践参考,以满足广大学习者的多样化需求,推动继续教育的快速发展。

真实工作场景下“搜索即学习”任务集的构建与分析

文章采用日记法收集来自16个国民经济行业的真实工作场景下具有学习特征的搜索任务实例,结合任务概念框架对搜索任务进行表征,构建面向“搜索即学习”研究的多维任务集,并进一步剖析了真实工作场景下搜索任务的属性特征,对任务相关特征、参与者认知变化、行为及关系进行探讨,根据参与者的学历层次、完成任务的认知层次,以及在完成任务时所处的时间压力进行分组比较三组变量间的关系。研究发现:参与者的任务执行熟悉度与任务感兴趣程度,所需信息类型的数量显著影响认知变化,任务执行者特征对认知变化与行为的影响在不同情境下均存在显著差异。本研究开发的任务集可作为“搜索即学习”研究的公共研究资源,以及评估生成式人工智能技术复杂任务处理能力的评测集,为开发面向真实工作任务情境与高效的信息系统提供依据。

我国公共图书馆参与开放教育资源运动初探

开放教育资源运动已开展二十余年,高校和研究型图书馆发挥教育职能,积极参与其中。对我国公共图书馆参与开放教育资源运动的可行性进行剖析,包括动机、机会与能力。针对公共图书馆会面临的版权、质量、配套资源等方面的挑战,提出制定政策、联合社会力量、关注基础教育、资源建设与服务、专业人员培训等方面的解决策略。

高校图书馆特色资源库建设方法和策略研究——以南京师范大学图书馆学人文库(校友文库)建设为例

高校图书馆特色资源建设不仅具有本校的学科特色,而且体现了高校图书馆的学术积淀和人文底蕴。高校图书馆加强学人文库(校友文库)的建设具有文化认同、提升学术水平、促进学术研究交流的重要意义。论文在分享南京师范大学图书馆学人文库(校友文库)文献收集和搭建设计校友文库平台虚拟平台的基础上,对高校图书馆文库建设和服务进行了展望,认为文库需要建立共享交流纽带才能充分调动校友捐赠的参与积极性;创新服务管理模式才能更加助力学术带头人获得更多成果;高效整合学术资源才能加深对校友学术资源的挖掘开发。