To obtain excellent regression results under the condition of small sample hyperspectral data,a deep neural network with simulated annealing(SA-DNN)is proposed.According to the characteristics of data,the attention me...To obtain excellent regression results under the condition of small sample hyperspectral data,a deep neural network with simulated annealing(SA-DNN)is proposed.According to the characteristics of data,the attention mechanism was applied to make the network pay more attention to effective features,thereby improving the operating efficiency.By introducing an improved activation function,the data correlation was reduced based on increasing the operation rate,and the problem of over-fitting was alleviated.By introducing simulated annealing,the network chose the optimal learning rate by itself,which avoided falling into the local optimum to the greatest extent.To evaluate the performance of the SA-DNN,the coefficient of determination(R^(2)),root mean square error(RMSE),and other metrics were used to evaluate the model.The results show that the performance of the SA-DNN is significantly better than other traditional methods.展开更多
Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of...Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of epiphyseal, articular and rib chondrocytes. Methods Sulfate GAG content in extracellular matrix of three chondrocytes was measured by the modified dimethylmethylene blue (DMB) method. The changes of the toluidine blue (TB) stain of chondrocytes were observed by light microscope. Results Primary chondrocytes had the highest content of sulfate GAG in the extracellular matrix, ie, epiphyseal chondrocytes reached ( 70. 12 ± 7. 72 )μg/cm2, articular chondrocytes (92.00 ± 10.15) μg/cm2 and rib chondrocytes (80.61 ± 11. 40) μg/cm2, respectively. On the third pasage chondrocytes, epiphyceal chondrocytes decreased to (53.27 ± 9. 50 ) μg/cm2, articular chondrocytes to (63.88 ± 11.92) μg/cm2 and rib chondrocytes to (58.94 ±8.21) μg/cm2, respectively. The change of TB in every passage展开更多
基金supported by the National Natural Science Foundation of China(Nos.62001023,61922013)Beijing Natural Science Foundation(No.4232013).
文摘To obtain excellent regression results under the condition of small sample hyperspectral data,a deep neural network with simulated annealing(SA-DNN)is proposed.According to the characteristics of data,the attention mechanism was applied to make the network pay more attention to effective features,thereby improving the operating efficiency.By introducing an improved activation function,the data correlation was reduced based on increasing the operation rate,and the problem of over-fitting was alleviated.By introducing simulated annealing,the network chose the optimal learning rate by itself,which avoided falling into the local optimum to the greatest extent.To evaluate the performance of the SA-DNN,the coefficient of determination(R^(2)),root mean square error(RMSE),and other metrics were used to evaluate the model.The results show that the performance of the SA-DNN is significantly better than other traditional methods.
文摘Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of epiphyseal, articular and rib chondrocytes. Methods Sulfate GAG content in extracellular matrix of three chondrocytes was measured by the modified dimethylmethylene blue (DMB) method. The changes of the toluidine blue (TB) stain of chondrocytes were observed by light microscope. Results Primary chondrocytes had the highest content of sulfate GAG in the extracellular matrix, ie, epiphyseal chondrocytes reached ( 70. 12 ± 7. 72 )μg/cm2, articular chondrocytes (92.00 ± 10.15) μg/cm2 and rib chondrocytes (80.61 ± 11. 40) μg/cm2, respectively. On the third pasage chondrocytes, epiphyceal chondrocytes decreased to (53.27 ± 9. 50 ) μg/cm2, articular chondrocytes to (63.88 ± 11.92) μg/cm2 and rib chondrocytes to (58.94 ±8.21) μg/cm2, respectively. The change of TB in every passage