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Optimization of Extraction Process for Total Flavonoids from Penthorum chinense Pursh and Comparison of Their Contents from Different Parts
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作者 Hongsheng LIU Jiqiang YAO +2 位作者 Xue BAI Jianying KANG Yefei YUAN 《Medicinal Plant》 2024年第2期10-14,共5页
[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experimen... [Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh. 展开更多
关键词 Penthorum chinense Pursh Total flavonoids Orthogonal experiments Extraction process Content determination
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TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone
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作者 Jiangcun WEI Xiumei MA +5 位作者 Meiyan QIU Bing QING Jingrong LU Hong LEI Xiaodong HUANG Wen ZHONG 《Medicinal Plant》 2024年第2期15-17,20,共4页
[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with ... [Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal. 展开更多
关键词 Pileostegia tomentellal TLC identification Extraction technology UMBELLIFERONE Content determination
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Determination of Chlorogenic Acid,Geniposide,Total Flavonoids and Total Triterpenes in Wulan-13
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作者 Xiaohua WU Burenbatu 《Medicinal Plant》 2024年第2期6-9,共4页
[Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposi... [Objectives]This study was conducted to establish a method for the determination of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13.[Methods]The contents of chlorogenic acid and geniposide were determined by HPLC,and the contents of total flavonoids and total triterpenes were determined by an ultraviolet spectrophotometer.[Results]There was a good linear relation between the mass of chlorogenic acid reference substance and the peak area in the range of 0.05-0.45μg,and the regression equation was Y=2524.1X+3.1943,(r=0.9998).A good linear relationship was found between the mass of gardenoside reference substance and the peak area in the range of 0.776-6.984μg,and the regression equation was Y=1670.5X+64.804,(r=0.9998).There was also a good linear relation between the mass of rutin reference substance and its absorbance in the range of 0.00808-0.04848 mg,and the regression equation was Y=12.916X+0.014,(r=0.999).The mass of oleanolic acid reference substance had a good linear relation with its absorbance in the range of 0.00418-0.0209 mg,and the regression equation was Y=51.89X-0.0839,(r=0.9991).[Conclusions]The content determination method is simple,reliable and reproducible,and suitable for controlling the contents of chlorogenic acid,geniposide,total flavonoids and total triterpenes in Wulan-13. 展开更多
关键词 Wulan-13 HPLC Ultraviolet spectrophotometer Content determination
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Research on Purification Processand PharmacologicalAction of Andrographolide
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作者 Changtao CAI Wenshuang HOU +3 位作者 Quan QUAN Jingchao WANG Mingxinzhi WANG Chenghao JIN 《Medicinal Plant》 2024年第5期13-16,共4页
This paper reviews the purification process,content determination methods and pharmacological action of Andrographolide,aiming to provide new ideas for the subsequent study of Andrographolide and its related drug deve... This paper reviews the purification process,content determination methods and pharmacological action of Andrographolide,aiming to provide new ideas for the subsequent study of Andrographolide and its related drug development and application. 展开更多
关键词 ANDROGRAPHOLIDE Purification process Content determination Pharmacological action
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Determination of Salvianolic Acid B in Yiqi Huayu Prescription by HPLC
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作者 Yingjing WANG Licheng SU +1 位作者 Yuliang HU Zhuqiang WANG 《Medicinal Plant》 2024年第1期32-34,共3页
[Objectives]To determine the content of salvianolic acid B in Yiqi Huayu Prescription by HPLC.[Methods]The chromatographic column was ZORBAX Eclipse Plus C 18(4.6 nm×250 nm,5μm);the mobile phase was acetonitrile... [Objectives]To determine the content of salvianolic acid B in Yiqi Huayu Prescription by HPLC.[Methods]The chromatographic column was ZORBAX Eclipse Plus C 18(4.6 nm×250 nm,5μm);the mobile phase was acetonitrile-0.1%phosphoric acid(21:79),the detection wavelength was 286 nm,the column temperature was 30℃,and the flow rate was 1.0 mL/min.A method for determination of salvianolic acid B in Yiqi Huayu Prescription was established.[Results]The linear relationship of salvianolic acid B was good in the range of 0.0214-0.4064 mg/mL.The regression equation was Y=5995.98984 X-0.07332,r=0.9999.The average recovery rate was 98.88%(RSD=1.6%).[Conclusions]The method is reliable,accurate and specific,and can be used for the determination of salvianolic acid B in Yiqi Huayu Prescription. 展开更多
关键词 Yiqi Huayu Prescription Salvianolic acid B High performance liquid chromatography Content determination
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Study of Quality Standards of Xiao’er Qingre Enema Preparation in Hospitals
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作者 Ruizhong Wang Yurong Zhang +3 位作者 Junjun Mao Shaoli Liang Yan Wu Lin Chen 《Journal of Clinical and Nursing Research》 2024年第1期125-132,共8页
Objective:To establish the quality standards for the preparation of Xiao’er Qingre enema in hospitals.Method:Thin-layer chromatography(TLC)was used to identify Radix Isatidis and Glycyrrhizae in the prescription.The ... Objective:To establish the quality standards for the preparation of Xiao’er Qingre enema in hospitals.Method:Thin-layer chromatography(TLC)was used to identify Radix Isatidis and Glycyrrhizae in the prescription.The content of(R,S)-Goitrin was determined by high-performance liquid chromatography(HPLC).Results:In TLC identification,there was no interference between the negative controls of Radix Isatidis and glycyrrhizae,and the spots were well-separated.The HPLC results of(R,S)-Goitrin showed a good linear relationship between the peak area and concentration within the range of 0.476-95.2μg·mL-1(r=0.9999).Conclusion:The TLC and HPLC methods established in this experiment are simple,reproducible,and specific,making them suitable for the quality control Xiao’er Qingre enema preparation in hospitals. 展开更多
关键词 Xiao’er Qingre enema (R S)-Goitrin TLC HPLC Content determination
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Determination of the Contents of Seven Chemical Components in Vidal Grape by Quantitative Analysis of Multi-components by Single Marker(QAMS) 被引量:1
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作者 Shizhao XU Fei QI +2 位作者 Yalin XI Ling WU Weijia SUN 《Agricultural Biotechnology》 CAS 2020年第6期131-135,共5页
[Objectives]This study was conducted to establish a method of quantitative analysis of multi-components by single marker(QAMS)for the simultaneous determination of such seven chemical components as gallic acid,epicate... [Objectives]This study was conducted to establish a method of quantitative analysis of multi-components by single marker(QAMS)for the simultaneous determination of such seven chemical components as gallic acid,epicatechin,catechin,ferulic acid,chlorogenic acid,rutin and caffeic acid in Vidal grape.[Methods]The high performance liquid chromatography was carried out using a COSMOSIL C18-MS-II column(4.6 mm×250 mm,5μm)with the mobile phase acetonitrile-2%acetic acid aqueous solution(gradient elution)at a flow rate of 1.0 ml/min.The detection wavelength was 280 nm,and the column temperature was 25℃.Using caffeic acid as an internal reference,the relative correction factors between it and other six to-be-detected components,and the contents of the seven components were calculated using the correction factors.The established was compared the results with the external standard method to verify the feasibility and accuracy of the method.[Results]The seven components had a good linear relationship in the ranges of 1.060-10.60,1.419-14.19,1.062-10.62,0.2950-2.950,0.1019-1.019,0.2014-2.014,and 0.1498-1.498μg,respectively,and the relative correction factors of gallic acid,epicatechin,catechin,ferulic acid,chlorogenic acid and rutin were 0.9760,0.7806,0.3277,1.640,1.161,2.778,respectively.There was no significant difference between the results of the QAMS method and the external standard method.[Conclusions]The QAMS method using caffeic acid as an internal reference is accurate and feasible,and provides a reliable method for the quality evaluation of Vidal ice grape. 展开更多
关键词 High performance liquid chromatography Vidal grape Content determination Polyphenol compounds
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Extraction Process and Quality Standard of Danshen Jianxin Capsules 被引量:1
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作者 Chengtong LIU Xinying MO +5 位作者 Silu HE Donglian CHEN Guilin YANG Hongsheng TAN Peng LIANG Haisheng ZENG 《Medicinal Plant》 CAS 2023年第1期25-28,共4页
[Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,ex... [Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,extraction time and extraction times as factors,and the content of tanshinone I as the index.Salviae Miltiorrhizae,Notoginseng Radix Et Rhizoma and Radix Astragali in the capsules were qualitatively identified by TLC,and the content of tanshinone I in the capsules was determined by HPLC.[Results]The best water extraction process included the steps of adding 12 times of water each time,extracting the materials twice,for 1 h each time.In TLC identification,the test samples showed spots of the same color at the positions corresponding to tanshinone II A,ginsenoside Rg1,notoginsenoside R1 and astragaloside IV reference samples.[Conclusions]Danshen Jianxin Capsules was prepared by the water extraction method,which is characterized by high efficiency and being suitable for mass production.The quality control method is reliable,fast and accurate,which can effectively control the quality of the product. 展开更多
关键词 Danshen Jianxin Capsules Extraction process Content determination TLC identification Quality standard
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Study on Quality Standard of Zhuang Medicine Buddlejae Flos
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作者 Tong LI Zhengsan LING +10 位作者 Xin XIE Liyuan LI Haifeng WEI Suoyi HUANG Shiyou ZHOU Lixiang LU Tingting HE Ran CHEN Yue LI Zheng LIU Jinchi LIU 《Medicinal Plant》 CAS 2023年第2期27-33,共7页
[Objectives]This study was conducted to explore the quality control method and establish the quality standard of Zhuang medicine Buddlejae Flos.[Methods]The microscopic identification method was adopted to identify th... [Objectives]This study was conducted to explore the quality control method and establish the quality standard of Zhuang medicine Buddlejae Flos.[Methods]The microscopic identification method was adopted to identify the characters and microscopic characteristics of dried Buddlejae Flos in combination with traditional experience.The moisture,ash,acid-insoluble components and extracts of Buddlejae Flos were determined with reference to general rules of Chinese Pharmacopoeia(2015 edition).TLC identification was performed.Liquid chromatography-mass spectrometry(LC-MS)was used to determine the contents of linarin and verbascoside in Buddlejae Flos,and gas chromatography-mass spectrometry(GC-MS)was adopted to detect pesticide residues in Buddlejae Flos.Heavy metal elements As,Cd and Pb were detected by inductively coupled plasma mass spectrometry(ICP-MS).[Results]The established method is simple and accurate.Clear spots were observed on the thin layer chromatograms,and the resolution was good.The average value of moisture content was 12.24%;the average value of ash content was 5.1%;the average value of acid-insoluble content was 7.5%;and the average value of extract content was 27.3%.The regression equation of rutin in Buddlejae Flos was y=11.896x-0.0049,R^(2)=0.9996,and the contents of linarin and verbascoside were 5680 and 2080 mg/kg,respectively.No pesticide residues and heavy metals were detected in the medicinal materials of Buddlejae Flos.[Conclusions]This study can provide reference for the quality standard control of Guangxi Zhuang medicine Buddlejae Flos. 展开更多
关键词 Zhuang Medicine Buddlejae Flos LC-MS GC-MS ICP-MS Quality standards Content determination
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Quality Evaluation of Streptocaulon griffithii Hook Based on Fingerprint and Quantitative Analysis of Multiple Components by the QAMS Method
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作者 Xinying MO Honghan QIN +3 位作者 Yudan LU Baoxian LIANG Hongsheng TAN Haisheng ZENG 《Medicinal Plant》 CAS 2023年第2期46-51,共6页
[Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStrepto... [Objectives]Chromatographic fingerprint analysis technology and quantitative analysis of multi-components by singlemarker(QAMS)were used to identify the authenticity and multi-component quantitative analysis ofStreptocaulon griffithiiHook,which provided experimental reference for the quality evaluation and control ofS.griffithiiHook.[Methods]Method was carried out on Agilent ZORBAX SB-C18(150 mm×4.6 mm,5μm)column with mobile phase composed of methanol-0.2%phosphoric acid solution at a flow rate of 1.0 mL/min in gradient elution mode.The column temperature was maintained at 30℃,the injection volume was 5μL,and the detection wavelengths were set at 230 nm.The HPLC fingerprint ofS.griffithiiHook was established byFingerprint Similarity Evaluation Software of Traditional Chinese Med-i cine(2012 edition),and the quality of 11 batches ofS.griffithiiHook extracts was analyzed.The content of chlorogenic acid,caffeic acid and4-methoxysalicylaldehyde inS.griffithiiHook was determined by QAMS.[Results]Thirteen common peaks were identified in the extract ofS.g riffithiiHook,and three components were identified as chlorogenic acid,caffeic acid and 4-methoxysalicylaldehyde,there was no significant difference between QAMS method and external standard method with chlorogenic acid as reference substance.[Conclusions]The established HPLC method is specific,accurate,stable and reproducible,and it can be used as an effective method for the quality control ofS.griffithii Hook. 展开更多
关键词 Streptocaulon griffithiiHook FINGERPRINT Content determination QAMS CHEMOMETRICS
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Purification Process and Biological Activity of Daphnoretin
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作者 Jinglong CAO Jian LIU +3 位作者 Yannan LI Hui XUE Yinghua LUO Chenghao JIN 《Plant Diseases and Pests》 CAS 2023年第1期34-36,共3页
Daphnoretin,belonging to coumarin compounds,is the main active ingredient of Wikstroemia indica,and has anti-inflammatory,anti-depression,anti-tumor and other pharmacological activities.This article reviews the extrac... Daphnoretin,belonging to coumarin compounds,is the main active ingredient of Wikstroemia indica,and has anti-inflammatory,anti-depression,anti-tumor and other pharmacological activities.This article reviews the extraction and purification process,content determination methods and pharmacological activity of daphnoretin,in order to provide a theoretical reference for optimization of purification process,improvement of content determination technique and further clinical application of daphnoretin. 展开更多
关键词 DAPHNORETIN Purification process Content determination Pharmacological activity
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Purification Process,Content Determination,Pharmacological Activity and Molecular Mechanism of Neogambogic Acid
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作者 Tong ZHANG Jinglong CAO +3 位作者 Wenshuang HOU Anqi WANG Yinghua LUO Chenghao JIN 《Plant Diseases and Pests》 CAS 2023年第2期32-35,共4页
Neogambogic acid is characterized by broad antitumor spectrum,good antitumor effect and low toxicity and side effects.This paper reviews the purification process,content determination and pharmacologic activity of neo... Neogambogic acid is characterized by broad antitumor spectrum,good antitumor effect and low toxicity and side effects.This paper reviews the purification process,content determination and pharmacologic activity of neogambogic acid,in order to provide a theoretical reference for the research and application of neogambogic acid. 展开更多
关键词 Neogambogic acid Purification process Content determination Pharmacological activity
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Determination of Total Flavonoids in Leaves of Tetracera asiatica
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作者 Fangfang DA Caoju MO +3 位作者 Wenxiu HUANG Yulin LU Qiying YANG Qing ZHANG 《Medicinal Plant》 CAS 2023年第4期31-34,共4页
[Objectives] This study was conducted to determine and compare the contents of flavonoids in the leaves of Tetracera asiatica , an ethnic medicine from different habitats, in order to provide a basis for the quality c... [Objectives] This study was conducted to determine and compare the contents of flavonoids in the leaves of Tetracera asiatica , an ethnic medicine from different habitats, in order to provide a basis for the quality control of its medicinal materials. [Methods] The flavonoid compounds in the leaves of T. asiatica were reflux-extracted in a water bath, and the total flavonoid content in T. asiatica was determined by ultraviolet-visible spectrophotometry. [Results] The total flavonoid content in T. asiatica leaves varied according to different habitats. The highest content was found in Gaofeng (19.41%) of Guangxi, followed by Huizhou (16.88%) of Guangdong and Hong Kong (16.76%). The lowest total flavonoid content was found in Shangcuntang (3.91%) of Bobai County, Guangxi, followed by Beiliu (4.15%), Guangxi. The total flavonoid contents in the samples ranged from 3.91% to 19.41%, with an average content of about 12.36%. [Conclusions] The UV spectrophotometry method is easy to operate, and has good repeatability, accurate detection results, and high reliability, and can be used for the determination of flavonoids in T. asiatica . 展开更多
关键词 Leaves of Tetracera asiatica Total flavonoids Content determination HABITAT
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Quality Standards of Carpesium lipskyi
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作者 Weiyu WANG Ruyin DONG +1 位作者 Xuemei LU Luyang LU 《Medicinal Plant》 CAS 2023年第3期33-37,共5页
[Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identifica... [Objectives]The paper was to establish the quality standard of Carpesium lipskyi,and to provide a scientific reference for its quality control and rational development and utilization.[Methods]Morphological identification,microscopic identification and thin layer chromatography(TLC)identification of the herbal samples were performed.The contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extract of the herbal samples were determined,and their total flavonoid content was determined by UV-spectrophotometry.[Results]The average contents of moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of 10 batches of C.lipskyi were 10.38%-12.27%,8.03%-14.72%,1.01%-3.06%and 20.84%-26.82%,respectively.The total flavonoids content ranged from 4.60%to 5.63%.[Conclusions]The established standard can provide a reference for the quality control of C.lipskyi.The tentative regulations stipulate that the moisture content of C.lipskyi shall not exceed 13.0%;the total ash content shall not exceed 15.0%;the acid-insoluble ash content shall not exceed 4.0%;the alcohol-soluble extract shall not be less than 16.0%;and the total flavonoids shall not be less than 3.0%. 展开更多
关键词 Carpesium lipskyi Winkl Quality standard Total flavonoids Content determination
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Study on Quality Standard of Hibiscus trionum L.
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作者 Quan MA Yang WEN +4 位作者 Yuebu HAILAI Yuan LIU Junyin LU Tao WANG Wenbing LI 《Medicinal Plant》 CAS 2023年第3期25-28,共4页
[Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.Th... [Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.The medicinal materials were qualitatively identified by microscopic identification and TLC,and the content of quercetin was determined by HPLC.[Results]The moisture,total ash,acid insoluble ash and extract contents of the 12 batches of samples were 7.69%-12.94%,10.44%-14.62%,1.89%-3.64%,16.56%-21.81%,microscopic characteristic was obvious,and a method for TLC and content determination of H.trionum L.was established.[Conclusions]A perfect quality standard was established,which can be used for the quality control of H.trionum L. 展开更多
关键词 Hibiscus trionum L. QUERCETIN Thin layer identification Content determination
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Extraction Process of Zhuang Medicine Fumigation Lotion
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作者 Jiangcun WEI Meiyan QIU +4 位作者 Bing QING Xianyi SHI Yinghong HUANG Xian PENG Wen ZHONG 《Medicinal Plant》 CAS 2023年第5期29-31,41,共4页
[Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amo... [Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amount of water added,decocting time and extraction times as factors,and syringin content and dry extract yield as indexes.The content of syringin was determined by high performance liquid chromatography.[Results]The best water extraction process was:soaking in water for 1 h,decocting twice,added 10 times the amount of water each time,decocting for 1 h.The average content of syringin in 3 batches was 0.98 mg/g,and the average dry extract yield was 26.07%.[Conclusions]The project adopts water extraction method to prepare Zhuang medicine fumigation lotion,which has the characteristics of high efficiency and suitable for large-scale production.The quality control method is reliable,rapid and accurate,and can effectively control the quality of the lotion. 展开更多
关键词 Zhuang medicine fumigation lotion Extraction process Content determination
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Research Progress on Purification Process Optimization and Content Determination of Zeaxanthin
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作者 Jian LIU Jinglong CAO +3 位作者 Hui XUE Yannan LI Wenshuang HOU Chenghao JIN 《Asian Agricultural Research》 2023年第3期37-39,46,共4页
At present,the purification process of zeaxanthin mainly includes organic solvent extraction,ultrasonic-assisted extraction and enzyme extraction,and the content determination technology mainly includes ultraviolet-sp... At present,the purification process of zeaxanthin mainly includes organic solvent extraction,ultrasonic-assisted extraction and enzyme extraction,and the content determination technology mainly includes ultraviolet-spectrophotometry and high performance liquid chromatography.In this paper,the purification process and content determination technology of zeaxanthin in recent years are reviewed in order to provide ideas and theoretical basis for further research and application of zeaxanthin. 展开更多
关键词 ZEAXANTHIN CAROTENOID Purification process Content determination
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Determination of the Content of Five Active Components in Toad Skin
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作者 Xue JIANG Wenli LI +1 位作者 Yong CHEN Jiabao MA 《Medicinal Plant》 CAS 2023年第4期38-40,共3页
[Objectives] To establish a method for the determination of active components in toad skin. [Methods] HPLC method was used to determine the content of five active components (bufotalin, cinobufotalin, bufalin, cinobuf... [Objectives] To establish a method for the determination of active components in toad skin. [Methods] HPLC method was used to determine the content of five active components (bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogenin) in toad skin. [Results] Chromatographic conditions are as follows: Agilent ZORBAX SB-C 18 chromatographic column was used;acetonitrile (A)-0.3% glacial acetic acid (B) gradient elution (0-15 min, 28%A-54%A;15-35 min, 54%A-54%A) was conducted;the flow rate was 0.6 mL/min;the detection wavelength was 296 nm;the column temperature was 30 ℃;the sample size was 10 μL. Under the above conditions, the determination method of the five components can be established at one time. [Conclusions] The method was stable and reliable, and can provide experimental basis for the development and utilization of active ingredients in toad skin. 展开更多
关键词 Toad skin High performance liquid chromatography Determination of content
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Detection and Analysis of Squalene in Oil from Olive Fruits of Different Varieties and of Different Degrees of Maturity 被引量:2
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作者 耿树香 宁德鲁 +2 位作者 张艳丽 陈海云 李勇杰 《Agricultural Science & Technology》 CAS 2013年第10期1482-1485,共4页
[Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the o... [Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the objective to provide technical support for the harvest and processing of olive fruits. [Method] Taking 13 introduced olive varieties including three varieties of eight different grades of maturity, fat acid w3s first extracted by Soxhlet extraction and then squalene was quantitatively and qualitatively analyzed by gas chromatography-mass spectrometry (GC-MS) method. [Result] The results showed that squalene content was the highest in CG-32, and the lowest in Leccino among the 13 olive varieties. And squalene content increased with the degree of maturity in the three olive varieties including CG-32, Frantoio and Ascolana Tenera. [Conclusion] A more simple and rapid method for the determina- tion of squatene content was established. 展开更多
关键词 OLIVE Olive oil Determination of squalene content GC-MS method
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Determination of Phytoene Content in Tomato Ketchup by HPLC
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作者 吴增宝 薛林 +3 位作者 田珊珊 朱启军 田洁 彭代萍 《Agricultural Science & Technology》 CAS 2016年第11期2627-2628,2635,共3页
[Objective] The aim was to establish an HPLC method for determination of phytoene content in tomato ketchup. [Method] The chromatographic conditions were as follows: column, Agilent AT C18 (250 mm×4.6 mm, 5 μm... [Objective] The aim was to establish an HPLC method for determination of phytoene content in tomato ketchup. [Method] The chromatographic conditions were as follows: column, Agilent AT C18 (250 mm×4.6 mm, 5 μm); mobile phase, methanol-THF (75:25); detection wavelength, 287 nm; flow rate, 1.0 ml/min; column temperature, 30 ℃; sample size, 50 μl. [Result] There was a good linear relation- ship in the phytoene content range of 0.186-1.116μg. The average recovery rate was 103.8% with RSD of 1.47%. The phytoene content in the tomato ketchup sample was determined as 10.7 rag/100 g simple, accurate, rapid and reliable, and phytoene content in tomato ketchup. [Conclusion] The established method is it can be used for the determination of 展开更多
关键词 TOMATO PHYTOENE HPLC Content determination
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